Restriction map for
pFN21AE2271
(Linear) (Six-base) MAPSORT of: KIEE2271.seq Check: 3223 from: 1 to: 1374
KIEE2271.orf
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 404 1374
Size: 404 970
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 787 1374
Size: 385 402 587
Fragments arranged by size:
587 402 385
AflII C'TTAA_G
Cuts at: 0 295 1374
Size: 295 1079
AflIII A'CryG_T
Cuts at: 0 1360 1374
Size: 1360 14
AgeI A'CCGG_T
Cuts at: 0 1346 1374
Size: 1346 28
AleI CACnn'nnGTG
Cuts at: 0 1177 1374
Size: 1177 197
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1374
Size: 574 800
AlwNI CAG_nnn'CTG
Cuts at: 0 414 1110 1134 1374
Size: 414 696 24 240
Fragments arranged by size:
696 414 240 24
ApoI r'AATT_y
Cuts at: 0 972 1374
Size: 972 402
AvaI C'yCGr_G
Cuts at: 0 1122 1374
Size: 1122 252
BanI G'GyrC_C
Cuts at: 0 538 1262 1268 1277 1374
Size: 538 724 6 9 97
Fragments arranged by size:
724 538 97 9 6
BanII G_rGCy'C
Cuts at: 0 199 436 527 1374
Size: 199 237 91 847
Fragments arranged by size:
847 237 199 91
BbeI G_GCGC'C
Cuts at: 0 1281 1374
Size: 1281 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 505 1333 1363 1374
Size: 311 194 828 30 11
Fragments arranged by size:
828 311 194 30 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1034 1068 1374
Size: 1034 34 306
Fragments arranged by size:
1034 306 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 96 1374
Size: 96 1278
BclI T'GATC_A
Cuts at: 0 281 1374
Size: 281 1093
BlpI GC'TnA_GC
Cuts at: 0 1018 1374
Size: 1018 356
Bme1580I G_kGCm'C
Cuts at: 0 1342 1374
Size: 1342 32
BmtI G_CTAG'C
Cuts at: 0 607 1374
Size: 607 767
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 974 1374
Size: 974 400
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 541 606 799 949 1051 1374
Size: 511 30 65 193 150 102 323
Fragments arranged by size:
511 323 193 150 102 65 30
Bpu10I CC'TnA_GC
Cuts at: 0 809 1007 1374
Size: 809 198 367
Fragments arranged by size:
809 367 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1374
Size: 228 1146
BsaI GGTCTCn'nnnn_
Cuts at: 0 1056 1374
Size: 1056 318
BsaHI Gr'CG_yC
Cuts at: 0 1211 1278 1374
Size: 1211 67 96
Fragments arranged by size:
1211 96 67
BsaWI w'CCGG_w
Cuts at: 0 1346 1374
Size: 1346 28
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 35 65 1374
Size: 35 30 1309
Fragments arranged by size:
1309 35 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 361 729 1303 1374
Size: 43 318 368 574 71
Fragments arranged by size:
574 368 318 71 43
BseYI C'CCAG_C
Cuts at: 0 57 87 1203 1374
Size: 57 30 1116 171
Fragments arranged by size:
1116 171 57 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1201 1374
Size: 1201 173
BsiHKAI G_wGCw'C
Cuts at: 0 527 1374
Size: 527 847
Bsp1286I G_dGCh'C
Cuts at: 0 199 436 527 1342 1374
Size: 199 237 91 815 32
Fragments arranged by size:
815 237 199 91 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 404 1339 1374
Size: 404 935 35
Fragments arranged by size:
935 404 35
BsrBI CCG'CTC
Cuts at: 0 837 1290 1374
Size: 837 453 84
Fragments arranged by size:
837 453 84
BsrDI GCAATG_nn'
Cuts at: 0 466 1374
Size: 466 908
BsrFI r'CCGG_y
Cuts at: 0 1216 1265 1346 1374
Size: 1216 49 81 28
Fragments arranged by size:
1216 81 49 28
BsrGI T'GTAC_A
Cuts at: 0 121 1374
Size: 121 1253
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1110 1374
Size: 1110 264
BstEII G'GTnAC_C
Cuts at: 0 795 1374
Size: 795 579
BstXI CCAn_nnnn'nTGG
Cuts at: 0 535 1374
Size: 535 839
Bsu36I CC'TnA_GG
Cuts at: 0 1088 1374
Size: 1088 286
BtsI GCAGTG_nn'
Cuts at: 0 708 1111 1374
Size: 708 403 263
Fragments arranged by size:
708 403 263
EarI CTCTTCn'nnn_
Cuts at: 0 133 1374
Size: 133 1241
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 739 1374
Size: 739 635
EcoICRI GAG'CTC
Cuts at: 0 525 1374
Size: 525 849
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 385 511 541 606 787 799 949 1051
Size: 385 126 30 65 181 12 150 102
Cuts at: 1051 1374
Size: 323
Fragments arranged by size:
385 323 181 150 126 102 65 30
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 400 853 1374
Size: 400 453 521
Fragments arranged by size:
521 453 400
EcoO109I rG'GnC_Cy
Cuts at: 0 804 994 1131 1374
Size: 804 190 137 243
Fragments arranged by size:
804 243 190 137
HaeII r_GCGC'y
Cuts at: 0 52 1281 1374
Size: 52 1229 93
Fragments arranged by size:
1229 93 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1279 1311 1374
Size: 1279 32 63
Fragments arranged by size:
1279 63 32
KasI G'GCGC_C
Cuts at: 0 1277 1374
Size: 1277 97
MfeI C'AATT_G
Cuts at: 0 355 1374
Size: 355 1019
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 191 1169 1374
Size: 191 978 205
Fragments arranged by size:
978 205 191
MslI CAynn'nnrTG
Cuts at: 0 598 1177 1374
Size: 598 579 197
Fragments arranged by size:
598 579 197
MspA1I CmG'CkG
Cuts at: 0 519 1374
Size: 519 855
NaeI GCC'GGC
Cuts at: 0 1218 1267 1374
Size: 1218 49 107
Fragments arranged by size:
1218 107 49
NarI GG'CG_CC
Cuts at: 0 1278 1374
Size: 1278 96
NgoMIV G'CCGG_C
Cuts at: 0 1216 1265 1374
Size: 1216 49 109
Fragments arranged by size:
1216 109 49
NheI G'CTAG_C
Cuts at: 0 603 1374
Size: 603 771
NspI r_CATG'y
Cuts at: 0 551 1364 1374
Size: 551 813 10
Fragments arranged by size:
813 551 10
PciI A'CATG_T
Cuts at: 0 1360 1374
Size: 1360 14
PflMI CCAn_nnn'nTGG
Cuts at: 0 749 1374
Size: 749 625
PfoI T'CCnGG_A
Cuts at: 0 527 1374
Size: 527 847
PpuMI rG'GwC_Cy
Cuts at: 0 804 994 1131 1374
Size: 804 190 137 243
Fragments arranged by size:
804 243 190 137
PvuII CAG'CTG
Cuts at: 0 519 1374
Size: 519 855
SacI G_AGCT'C
Cuts at: 0 527 1374
Size: 527 847
SanDI GG'GwC_CC
Cuts at: 0 994 1374
Size: 994 380
SapI GCTCTTCn'nnn_
Cuts at: 0 133 1374
Size: 133 1241
SexAI A'CCwGG_T
Cuts at: 0 938 1374
Size: 938 436
SfoI GGC'GCC
Cuts at: 0 1279 1374
Size: 1279 95
SmlI C'TyrA_G
Cuts at: 0 243 295 1374
Size: 243 52 1079
Fragments arranged by size:
1079 243 52
StuI AGG'CCT
Cuts at: 0 619 859 1374
Size: 619 240 515
Fragments arranged by size:
619 515 240
StyI C'CwwG_G
Cuts at: 0 264 425 472 1374
Size: 264 161 47 902
Fragments arranged by size:
902 264 161 47
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 406 1065 1374
Size: 406 659 309
Fragments arranged by size:
659 406 309
TatI w'GTAC_w
Cuts at: 0 121 1374
Size: 121 1253
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 1374
Size: 65 32 1277
Fragments arranged by size:
1277 65 32
Enzymes that do cut:
AarI AcuI AflII AflIII AgeI AleI AlfI AlwNI
ApoI AvaI BanI BanII BbeI BbsI BcgI BciVI
BclI BlpI Bme1580I BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI Bsu36I BtsI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI MfeI MmeI MslI MspA1I
NaeI NarI NgoMIV NheI NspI PciI PflMI PfoI
PpuMI PvuII SacI SanDI SapI SexAI SfoI SmlI
StuI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AhdI AloI ApaI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BfrBI BglI BglII BmgBI BmrI BsaAI BsaBI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI
BstYI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
DrdI EaeI EagI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KpnI MluI MscI NcoI
NdeI NotI NruI NsiI PacI PasI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SbfI ScaI SfcI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI