################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglI BglII BlpI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeII MboII MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvnI MwoI NaeI NarI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI TfiI Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AciI 432 GCGG CCGC(-3/-1) AfaI 255 GTAC GT^AC AgeI 316 ACCGGT A^CCGGT AspI 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 405 GGACC G^G[AT]CC AvrII 360 CCTAGG C^CTAGG BanI 364 GGTGCC G^G[CT][GA]CC BcnI 5 CCGGG CC^[GC]GG BfaI 361 CTAG C^TAG BlnI 360 CCTAGG C^CTAGG BsaBI 394 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 316 ACCGGT [AT]^CCGG[AT] BsmAI 31 GTCTC GTCTC(1/5) Bsp1407I 254 TGTACA T^GTACA BsrFI 316 ACCGGT [GA]^CCGG[CT] BsrGI 254 TGTACA T^GTACA BsrI 121 ACTGG ACTGG(1/-1) BstEII 125 GGTCACC G^GT[ACGT]ACC BstNI 369 CCTGG CC^[AT]GG BstPI 125 GGTCACC G^GT[ACGT]ACC Cac8I 21 GCTGGC GC[ACGT]^[ACGT]GC Cfr10I 316 ACCGGT [GA]^CCGG[CT] Cfr13I 405 GGACC G^G[ACGT]CC EcoO65I 125 GGTCACC G^GT[ACGT]ACC EcoRII 369 CCTGG ^CC[AT]GG EcoT14I 360 CCTAGG C^C[AT][AT]GG HincII 351 GTTGAC GT[CT]^[GA]AC HindII 351 GTTGAC GT[CT]^[GA]AC HinfI 330 GAGTC G^A[ACGT]TC MaeI 361 CTAG C^TAG MamI 394 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 91 TTAA T^TAA MvaI 369 CCTGG CC^[AT]GG NciI 5 CCGGG CC^[GC]GG NlaIV 364 GGTGCC GG[ACGT]^[ACGT]CC PflFI 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 316 ACCGGT A^CCGGT PleI 330 GAGTC GAGTC(4/5) RsaI 255 GTAC GT^AC Sau96I 405 GGACC G^G[ACGT]CC SspBI 254 TGTACA T^GTACA SspI 412 AATATT AAT^ATT StyI 360 CCTAGG C^C[AT][AT]GG TaqI 13 TCGA T^CGA Tru9I 91 TTAA T^TAA TspRI 120 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 326 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 13 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 297 AGCT AG^CT 385 AGCT AlwI 15 GATCC GGATC(4/5) 311 GGATC AlwNI 116 CAGTCACTG CAG[ACGT][ACGT][ACGT]^CTG 293 CAGAAGCTG BbvI 25 GCTGC GCAGC(8/12) 298 GCTGC BmyI 152 GGGCAC G[AGT]GC[ACT]^C 365 GTGCCC Bsp1286I 152 GGGCAC G[AGT]GC[ACT]^C 365 GTGCCC BssKI 5 CCGGG ^CC[ACGT]GG 369 CCTGG BstF5I 203 GGATG GGATG(2/0) 393 GGATG DdeI 328 CTGAG C^T[ACGT]AG 427 CTGAG DpnII 15 GATC ^GATC 312 GATC EaeI 2 TGGCCG [CT]^GGCC[GA] 264 TGGCCG FokI 203 GGATG GGATG(9/13) 393 GGATG HapII 5 CCGG C^CGG 317 CCGG HpaII 5 CCGG C^CGG 317 CCGG MboI 15 GATC ^GATC 312 GATC MspI 5 CCGG C^CGG 317 CCGG NdeII 15 GATC ^GATC 312 GATC Sau3AI 15 GATC ^GATC 312 GATC ScrFI 5 CCGGG CC^[ACGT]GG 369 CCTGG TseI 25 GCTGC G^C[AT]GC 298 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 9 enzymes) # ######################################### BsaJI Fnu4HI HaeIII HphI ItaI MaeIII MnlI Tsp45I Tsp509I