################################## # NO CUT ENZYMES ( 166 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DpnII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HpaI HphI KpnI Ksp632I KspI MaeII MamI MboI MboII MfeI MflI MluI MluNI MscI MslI MunI MvnI NaeI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI TspRI Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 423 TCCGGA T^CCGGA AcyI 7 GGCGCC G[GA]^CG[CT]C AfaI 311 GTAC GT^AC BanI 7 GGCGCC G^G[CT][GA]CC BanII 253 GAGCCC G[GA]GC[CT]^C BbeI 7 GGCGCC GGCGC^C BciVI 320 GTATCC GTATCC(6/5) BmyI 253 GAGCCC G[AGT]GC[ACT]^C BsaHI 7 GGCGCC G[GA]^CG[CT]C BsaWI 423 TCCGGA [AT]^CCGG[AT] BseAI 423 TCCGGA T^CCGGA BseRI 148 GAGGAG GAGGAG(10/8) BsgI 67 CTGCAC GTGCAG(16/14) BsiYI 90 CCCCGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 90 CCCCGCCGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 139 GAGAC GTCTC(1/5) Bsp1286I 253 GAGCCC G[AGT]GC[ACT]^C BspEI 423 TCCGGA T^CCGGA BsrBI 11 CCGCTC CCGCTC(-3/-3) BstF5I 378 CATCC GGATG(2/0) DdeI 388 CTTAG C^T[ACGT]AG DraI 399 TTTAAA TTT^AAA DraII 348 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 32 TGGCCG [CT]^GGCC[GA] EcoO109I 348 AGGACCT [GA]G^G[ACGT]CC[CT] EcoT14I 126 CCATGG C^C[AT][AT]GG FokI 378 CATCC GGATG(9/13) HaeII 7 GGCGCC [GA]GCGC^[CT] Hin1I 7 GGCGCC G[GA]^CG[CT]C HinfI 413 GAATC G^A[ACGT]TC KasI 7 GGCGCC G^GCGCC MroI 423 TCCGGA T^CCGGA MspA1I 21 CCGCTG C[AC]G^C[GT]G NarI 7 GGCGCC GG^CGCC NcoI 126 CCATGG C^CATGG NspI 283 GCATGC [GA]CATG^[CT] PaeR7I 56 CTCGAG C^TCGAG PpuMI 348 AGGACCT [GA]G^G[AT]CC[CT] RsaI 311 GTAC GT^AC SfoI 7 GGCGCC GGC^GCC SmaI 44 CCCGGG CCC^GGG SmlI 56 CTCGAG C^T[CT][GA]AG SphI 283 GCATGC GCATG^C StuI 60 AGGCCT AGG^CCT StyI 126 CCATGG C^C[AT][AT]GG TaqI 57 TCGA T^CGA TfiI 413 GAATC G^A[AT]TC Tsp45I 132 GTCAC ^GT[GC]AC Tsp509I 206 AATT ^AATT TthHB8I 57 TCGA T^CGA XhoI 56 CTCGAG C^TCGAG XmaCI 44 CCCGGG C^CCGGG XmaI 44 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AvaI 44 CCCGGG C^[CT]CG[GA]G 56 CTCGAG AvaII 80 GGACC G^G[AT]CC 349 GGACC BfaI 308 CTAG C^TAG 325 CTAG BsoBI 44 CCCGGG C^[CT]CG[GA]G 56 CTCGAG BstNI 200 CCTGG CC^[AT]GG 381 CCAGG CfoI 8 GCGC GCG^C 26 GCGC DsaI 95 CCGTGG C^C[GA][CT]GG 126 CCATGG EcoRII 200 CCTGG ^CC[AT]GG 381 CCAGG FauI 91 CCCGC CCCGC(4/6) 279 GCGGG HhaI 8 GCGC GCG^C 26 GCGC HinP1I 8 GCGC G^CGC 26 GCGC MaeI 308 CTAG C^TAG 325 CTAG MvaI 200 CCTGG CC^[AT]GG 381 CCAGG MwoI 28 GCTGTGGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 274 GCTTTGCGGGC NlaIII 127 CATG CATG^ 284 CATG ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AciI BbvI BcnI BsaJI BsrI BssKI Cac8I Cfr13I Fnu4HI HaeIII HapII HpaII ItaI MaeIII MnlI MseI MspI NciI NlaIV Sau96I ScrFI SfaNI Tru9I TseI