(Circular) (Six-base) MAPSORT of: pFN21AE6109.seq Check: 6236 from: 1 to: 5155 pFN21AE6109 5155 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2965 278 Size: 53 83 186 936 1429 2468 Fragments arranged by size: 2468 1429 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2498 1937 Size: 561 4594 Acc65I G'GTAC_C Cuts at: 2476 2476 Size: 5155 AclI AA'CG_TT Cuts at: 1563 3282 3655 1563 Size: 1719 373 3063 Fragments arranged by size: 3063 1719 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3218 4602 4870 1632 Size: 1586 1384 268 1917 Fragments arranged by size: 1917 1586 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4958 AflIII A'CryG_T Cuts at: 1203 4054 5031 1203 Size: 2851 977 1327 Fragments arranged by size: 2851 1327 977 AgeI A'CCGG_T Cuts at: 3986 3986 Size: 5155 AhdI GACnn_n'nnGTC Cuts at: 3884 3884 Size: 5155 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5155 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5155 AlwNI CAG_nnn'CTG Cuts at: 2898 4470 2898 Size: 1572 3583 ApaLI G'TGCA_C Cuts at: 3212 4368 3212 Size: 1156 3999 ApoI r'AATT_y Cuts at: 1493 2464 2690 2842 4951 1493 Size: 971 226 152 2109 1697 Fragments arranged by size: 2109 1697 971 226 152 AseI AT'TA_AT Cuts at: 160 1032 3709 160 Size: 872 2677 1606 Fragments arranged by size: 2677 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5155 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2061 2480 1870 Size: 73 106 12 419 4545 Fragments arranged by size: 4545 419 106 73 12 BamHI G'GATC_C Cuts at: 2485 2852 2485 Size: 367 4788 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2012 2476 3931 618 Size: 325 217 627 225 464 1455 1842 Fragments arranged by size: 1842 1455 627 464 325 225 217 BanII G_rGCy'C Cuts at: 1391 2262 2474 1391 Size: 871 212 4072 Fragments arranged by size: 4072 871 212 BbeI G_GCGC'C Cuts at: 2016 2016 Size: 5155 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3346 3380 1903 Size: 34 50 34 1325 34 3678 Fragments arranged by size: 3678 1325 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2336 3047 4257 2336 Size: 711 1210 3234 Fragments arranged by size: 3234 1210 711 BclI T'GATC_A Cuts at: 1548 1548 Size: 5155 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3766 136 Size: 107 122 71 631 605 2094 1525 Fragments arranged by size: 2094 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5150 5150 Size: 5155 BlpI GC'TnA_GC Cuts at: 2572 2572 Size: 5155 Bme1580I G_kGCm'C Cuts at: 1790 3216 4372 1790 Size: 1426 1156 2573 Fragments arranged by size: 2573 1426 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5155 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2423 2447 2886 Size: 416 53 83 399 24 1006 24 439 Cuts at: 2886 3844 442 Size: 958 1753 Fragments arranged by size: 1753 1006 958 439 416 399 83 53 24 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5155 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3815 1239 Size: 2576 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3280 4145 4443 3280 Size: 865 298 3992 Fragments arranged by size: 3992 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3818 882 Size: 617 2319 2219 Fragments arranged by size: 2319 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2946 493 Size: 711 1742 2702 Fragments arranged by size: 2702 1742 711 BsaBI GATnn'nnATC Cuts at: 5149 5149 Size: 5155 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2013 2962 3344 3979 Size: 53 83 186 936 480 949 382 635 Cuts at: 3979 275 Size: 1451 Fragments arranged by size: 1451 949 936 635 480 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2428 3588 3986 4260 4407 4818 2428 Size: 1160 398 274 147 411 2765 Fragments arranged by size: 2765 1160 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4930 Fragments arranged by size: 4930 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2168 1186 Size: 982 4173 BseYI C'CCAG_C Cuts at: 2008 2169 4358 4854 2008 Size: 161 2189 496 2309 Fragments arranged by size: 2309 2189 496 161 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2057 1163 Size: 573 321 4261 Fragments arranged by size: 4261 573 321 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2605 3366 4394 1540 Size: 275 186 604 761 1028 2301 Fragments arranged by size: 2301 1028 761 604 275 186 BsiHKAI G_wGCw'C Cuts at: 3216 3301 4372 3216 Size: 85 1071 3999 Fragments arranged by size: 3999 1071 85 BsmI GAATG_Cn' Cuts at: 2666 2759 2666 Size: 93 5062 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2262 2474 3216 3301 4372 1391 Size: 399 472 212 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 472 399 212 85 BspEI T'CCGG_A Cuts at: 2428 4818 2428 Size: 2390 2765 BspHI T'CATG_A Cuts at: 3044 4774 3044 Size: 1730 3425 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2510 844 Size: 1666 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2018 3042 1394 Size: 429 195 1024 3507 Fragments arranged by size: 3507 1024 429 195 BsrDI GCAATG_nn' Cuts at: 65 1248 3650 3824 65 Size: 1183 2402 174 1396 Fragments arranged by size: 2402 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3799 3986 1700 Size: 110 1989 187 2869 Fragments arranged by size: 2869 1989 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5155 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5155 BssSI C'ACGA_G Cuts at: 3215 4227 3215 Size: 1012 4143 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5155 BstBI TT'CG_AA Cuts at: 3959 3959 Size: 5155 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5155 BstYI r'GATC_y Cuts at: 1972 2485 2852 3237 3254 4706 4792 4804 5150 Size: 513 367 385 17 1452 86 12 346 Cuts at: 5150 1972 Size: 1977 Fragments arranged by size: 1977 1452 513 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 2131 513 Size: 552 1035 31 3537 Fragments arranged by size: 3537 1035 552 31 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4416 Fragments arranged by size: 4416 683 56 BtsI GCAGTG_nn' Cuts at: 2671 3465 3485 2671 Size: 794 20 4341 Fragments arranged by size: 4341 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5155 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5120 DraI TTT'AAA Cuts at: 2406 2459 2406 Size: 53 5102 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4162 809 Size: 507 2846 1802 Fragments arranged by size: 2846 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2602 3491 Size: 54 1425 50 275 18 207 565 889 Cuts at: 3491 5120 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 565 275 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2602 1537 Size: 275 790 4090 Fragments arranged by size: 4090 790 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3085 1353 Size: 1732 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2012 2068 3742 4116 4262 387 Size: 1064 138 423 56 1674 374 146 1280 Fragments arranged by size: 1674 1280 1064 423 374 146 138 56 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3218 3815 4602 4870 1239 Size: 393 1586 597 787 268 1524 Fragments arranged by size: 1586 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2354 2354 Size: 5155 EcoRI G'AATT_C Cuts at: 2464 2842 2464 Size: 378 4777 FspI TGC'GCA Cuts at: 3661 3661 Size: 5155 HaeII r_GCGC'y Cuts at: 1678 2016 4302 1678 Size: 338 2286 2531 Fragments arranged by size: 2531 2286 338 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3796 3828 3870 3902 1373 Size: 32 135 32 2224 32 42 32 2626 Fragments arranged by size: 2626 2224 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2499 2745 669 Size: 988 281 561 246 3079 Fragments arranged by size: 3079 988 561 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5155 HpaI GTT'AAC Cuts at: 2745 2745 Size: 5155 KasI G'GCGC_C Cuts at: 2012 2012 Size: 5155 KpnI G_GTAC'C Cuts at: 2480 2480 Size: 5155 MfeI C'AATT_G Cuts at: 2754 2754 Size: 5155 MluI A'CGCG_T Cuts at: 5031 5031 Size: 5155 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4269 4453 1305 Size: 2964 184 2007 Fragments arranged by size: 2964 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3333 Fragments arranged by size: 3333 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3113 3472 3631 518 Size: 648 1947 359 159 2042 Fragments arranged by size: 2042 1947 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2028 2571 3248 4396 4641 1606 Size: 422 543 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 543 422 245 NarI GG'CG_CC Cuts at: 2013 2013 Size: 5155 NcoI C'CATG_G Cuts at: 513 1065 2131 513 Size: 552 1066 3537 Fragments arranged by size: 3537 1066 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5155 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5155 NotI GC'GGCC_GC Cuts at: 2602 2602 Size: 5155 NspI r_CATG'y Cuts at: 1132 1229 2292 2513 4058 1132 Size: 97 1063 221 1545 2229 Fragments arranged by size: 2229 1545 1063 221 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5155 PciI A'CATG_T Cuts at: 4054 4054 Size: 5155 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5155 PfoI T'CCnGG_A Cuts at: 2385 2385 Size: 5155 PmeI GTTT'AAAC Cuts at: 2459 2459 Size: 5155 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3224 3256 4737 4769 3224 Size: 32 1481 32 3610 Fragments arranged by size: 3610 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2354 2354 Size: 5155 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5155 PsiI TTA'TAA Cuts at: 2725 2725 Size: 5155 PspXI vC'TCGA_Gb Cuts at: 2061 2061 Size: 5155 PstI C_TGCA'G Cuts at: 830 2507 830 Size: 1677 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5155 SalI G'TCGA_C Cuts at: 1936 2497 1936 Size: 561 4594 SbfI CC_TGCA'GG Cuts at: 2507 2507 Size: 5155 ScaI AGT'ACT Cuts at: 3403 5115 3403 Size: 1712 3443 SfcI C'TryA_G Cuts at: 826 1045 2503 3638 4319 4510 826 Size: 219 1458 1135 681 191 1471 Fragments arranged by size: 1471 1458 1135 681 219 191 SfoI GGC'GCC Cuts at: 2014 2014 Size: 5155 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5155 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5155 SmaI CCC'GGG Cuts at: 1872 2051 2482 1872 Size: 179 431 4545 Fragments arranged by size: 4545 431 179 SmlI C'TyrA_G Cuts at: 820 1017 1943 2061 3259 4160 4422 820 Size: 197 926 118 1198 901 262 1553 Fragments arranged by size: 1553 1198 926 901 262 197 118 SnaBI TAC'GTA Cuts at: 493 2946 493 Size: 2453 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5155 SphI G_CATG'C Cuts at: 1132 2292 2513 1132 Size: 1160 221 3774 Fragments arranged by size: 3774 1160 221 SspI AAT'ATT Cuts at: 5 52 3079 5 Size: 47 3027 2081 Fragments arranged by size: 3027 2081 47 StuI AGG'CCT Cuts at: 1517 2067 1517 Size: 550 4605 StyI C'CwwG_G Cuts at: 513 1065 2131 2594 513 Size: 552 1066 463 3074 Fragments arranged by size: 3074 1066 552 463 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3182 3199 3352 3537 3982 325 Size: 1255 1602 17 153 185 445 1498 Fragments arranged by size: 1602 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2315 3401 5113 Size: 274 80 33 51 1443 338 1086 1712 Cuts at: 5113 96 Size: 138 Fragments arranged by size: 1712 1443 1086 338 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5115 5147 5115 Size: 32 5123 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5155 XbaI T'CTAG_A Cuts at: 2491 2491 Size: 5155 XhoI C'TCGA_G Cuts at: 1943 2061 1943 Size: 118 5037 XmaI C'CCGG_G Cuts at: 1870 2049 2480 1870 Size: 179 431 4545 Fragments arranged by size: 4545 431 179 XmnI GAAnn'nnTTC Cuts at: 3284 3284 Size: 5155 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2963 276 Size: 53 83 186 936 1429 2468 Fragments arranged by size: 2468 1429 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI