(Linear) (Six-base) MAPSORT of: KIEE1437.seq Check: 2962 from: 1 to: 3033 KIEE1437.orf With 182 enzymes: SgfI * July 15, 2010 18:38 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1096 3033 Size: 1096 1937 AccI GT'mk_AC Cuts at: 0 1343 1492 3027 3033 Size: 1343 149 1535 6 Fragments arranged by size: 1535 1343 149 6 Acc65I G'GTAC_C Cuts at: 0 1171 3033 Size: 1171 1862 AclI AA'CG_TT Cuts at: 0 2615 3033 Size: 2615 418 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 581 3033 Size: 581 2452 AflII C'TTAA_G Cuts at: 0 1335 3033 Size: 1335 1698 AflIII A'CryG_T Cuts at: 0 1787 3033 Size: 1787 1246 AhdI GACnn_n'nnGTC Cuts at: 0 287 3033 Size: 287 2746 AleI CACnn'nnGTG Cuts at: 0 2071 2640 3033 Size: 2071 569 393 Fragments arranged by size: 2071 569 393 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 418 902 3033 Size: 418 484 2131 Fragments arranged by size: 2131 484 418 AlwNI CAG_nnn'CTG Cuts at: 0 1582 3033 Size: 1582 1451 ApaI G_GGCC'C Cuts at: 0 970 3033 Size: 970 2063 ApoI r'AATT_y Cuts at: 0 136 623 1520 2465 2536 2580 3033 Size: 136 487 897 945 71 44 453 Fragments arranged by size: 945 897 487 453 136 71 44 AseI AT'TA_AT Cuts at: 0 2207 3033 Size: 2207 826 AvaI C'yCGr_G Cuts at: 0 647 3033 Size: 647 2386 BamHI G'GATC_C Cuts at: 0 1364 3033 Size: 1364 1669 BanI G'GyrC_C Cuts at: 0 1171 1914 2055 3033 Size: 1171 743 141 978 Fragments arranged by size: 1171 978 743 141 BanII G_rGCy'C Cuts at: 0 505 935 970 1974 2113 2317 3033 Size: 505 430 35 1004 139 204 716 Fragments arranged by size: 1004 716 505 430 204 139 35 BbvCI CC'TCA_GC Cuts at: 0 1481 3033 Size: 1481 1552 BclI T'GATC_A Cuts at: 0 2126 3033 Size: 2126 907 BglII A'GATC_T Cuts at: 0 2489 3033 Size: 2489 544 BlpI GC'TnA_GC Cuts at: 0 2509 2741 3033 Size: 2509 232 292 Fragments arranged by size: 2509 292 232 Bme1580I G_kGCm'C Cuts at: 0 970 3033 Size: 970 2063 BmgBI CAC'GTC Cuts at: 0 1377 1788 3033 Size: 1377 411 1245 Fragments arranged by size: 1377 1245 411 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 919 1450 1825 2401 3033 Size: 91 828 531 375 576 632 Fragments arranged by size: 828 632 576 531 375 91 Bpu10I CC'TnA_GC Cuts at: 0 1481 3033 Size: 1481 1552 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 752 1573 1840 2906 3033 Size: 752 821 267 1066 127 Fragments arranged by size: 1066 821 752 267 127 BsaI GGTCTCn'nnnn_ Cuts at: 0 274 1760 3033 Size: 274 1486 1273 Fragments arranged by size: 1486 1273 274 BsaAI yAC'GTr Cuts at: 0 819 3033 Size: 819 2214 BsaHI Gr'CG_yC Cuts at: 0 974 2214 3033 Size: 974 1240 819 Fragments arranged by size: 1240 974 819 BsaWI w'CCGG_w Cuts at: 0 1361 2146 2948 3033 Size: 1361 785 802 85 Fragments arranged by size: 1361 802 785 85 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1130 1160 2144 2174 3033 Size: 1130 30 984 30 859 Fragments arranged by size: 1130 984 859 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 467 919 922 943 1060 1822 2165 Size: 21 446 452 3 21 117 762 343 Cuts at: 2165 2466 2744 3033 Size: 301 278 289 Fragments arranged by size: 762 452 446 343 301 289 278 117 21 21 3 BseYI C'CCAG_C Cuts at: 0 1440 1631 2106 3033 Size: 1440 191 475 927 Fragments arranged by size: 1440 927 475 191 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1221 2111 3033 Size: 1221 890 922 Fragments arranged by size: 1221 922 890 BsiHKAI G_wGCw'C Cuts at: 0 654 833 881 935 2168 2317 3033 Size: 654 179 48 54 1233 149 716 Fragments arranged by size: 1233 716 654 179 149 54 48 Bsp1286I G_dGCh'C Cuts at: 0 505 654 833 881 935 970 1974 2113 Size: 505 149 179 48 54 35 1004 139 Cuts at: 2113 2168 2317 3033 Size: 55 149 716 Fragments arranged by size: 1004 716 505 179 149 149 139 55 54 48 35 BspEI T'CCGG_A Cuts at: 0 2146 2948 3033 Size: 2146 802 85 Fragments arranged by size: 2146 802 85 BspHI T'CATG_A Cuts at: 0 2802 3033 Size: 2802 231 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1096 3033 Size: 1096 1937 BssSI C'ACGA_G Cuts at: 0 880 1292 3033 Size: 880 412 1741 Fragments arranged by size: 1741 880 412 BstXI CCAn_nnnn'nTGG Cuts at: 0 2601 3033 Size: 2601 432 BstYI r'GATC_y Cuts at: 0 605 1364 2489 3033 Size: 605 759 1125 544 Fragments arranged by size: 1125 759 605 544 BstZ17I GTA'TAC Cuts at: 0 1493 3033 Size: 1493 1540 Bsu36I CC'TnA_GG Cuts at: 0 1369 2449 3033 Size: 1369 1080 584 Fragments arranged by size: 1369 1080 584 BtgI C'CryG_G Cuts at: 0 1992 3033 Size: 1992 1041 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1976 3033 Size: 1976 1057 BtsI GCAGTG_nn' Cuts at: 0 402 3033 Size: 402 2631 ClaI AT'CG_AT Cuts at: 0 861 3033 Size: 861 2172 DraI TTT'AAA Cuts at: 0 1565 1589 2418 3033 Size: 1565 24 829 615 Fragments arranged by size: 1565 829 615 24 DraIII CAC_nnn'GTG Cuts at: 0 751 3033 Size: 751 2282 DrdI GACnn_nn'nnGTC Cuts at: 0 1417 3033 Size: 1417 1616 EaeI y'GGCC_r Cuts at: 0 1008 2063 2870 3033 Size: 1008 1055 807 163 Fragments arranged by size: 1055 1008 807 163 EarI CTCTTCn'nnn_ Cuts at: 0 569 2365 3033 Size: 569 1796 668 Fragments arranged by size: 1796 668 569 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1723 3033 Size: 1723 1310 EcoICRI GAG'CTC Cuts at: 0 933 2315 3033 Size: 933 1382 718 Fragments arranged by size: 1382 933 718 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 91 581 919 1450 1825 2401 3033 Size: 91 490 338 531 375 576 632 Fragments arranged by size: 632 576 531 490 375 338 91 EcoNI CCTnn'n_nnAGG Cuts at: 0 1676 1777 3033 Size: 1676 101 1256 Fragments arranged by size: 1676 1256 101 EcoO109I rG'GnC_Cy Cuts at: 0 591 967 1437 2121 2720 3033 Size: 591 376 470 684 599 313 Fragments arranged by size: 684 599 591 470 376 313 EcoRI G'AATT_C Cuts at: 0 2580 3033 Size: 2580 453 FspI TGC'GCA Cuts at: 0 11 2854 3033 Size: 11 2843 179 Fragments arranged by size: 2843 179 11 FspAI rTGC'GCAy Cuts at: 0 2854 3033 Size: 2854 179 HaeII r_GCGC'y Cuts at: 0 1983 3033 Size: 1983 1050 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 273 305 1774 1806 2784 2816 3033 Size: 273 32 1469 32 978 32 217 Fragments arranged by size: 1469 978 273 217 32 32 32 HincII GTy'rAC Cuts at: 0 3028 3033 Size: 3028 5 HindIII A'AGCT_T Cuts at: 0 125 3033 Size: 125 2908 KpnI G_GTAC'C Cuts at: 0 1175 3033 Size: 1175 1858 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1856 2265 3033 Size: 1856 409 768 Fragments arranged by size: 1856 768 409 MscI TGG'CCA Cuts at: 0 1010 2065 3033 Size: 1010 1055 968 Fragments arranged by size: 1055 1010 968 MslI CAynn'nnrTG Cuts at: 0 296 884 985 2053 2071 2599 2640 2942 Size: 296 588 101 1068 18 528 41 302 Cuts at: 2942 3033 Size: 91 Fragments arranged by size: 1068 588 528 302 296 101 91 41 18 MspA1I CmG'CkG Cuts at: 0 1994 3033 Size: 1994 1039 NspI r_CATG'y Cuts at: 0 783 3033 Size: 783 2250 PasI CC'CwG_GG Cuts at: 0 318 3033 Size: 318 2715 PflMI CCAn_nnn'nTGG Cuts at: 0 2284 3033 Size: 2284 749 PfoI T'CCnGG_A Cuts at: 0 760 935 1802 3033 Size: 760 175 867 1231 Fragments arranged by size: 1231 867 760 175 PmlI CAC'GTG Cuts at: 0 819 3033 Size: 819 2214 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2741 2773 3033 Size: 2741 32 260 Fragments arranged by size: 2741 260 32 PpuMI rG'GwC_Cy Cuts at: 0 591 1437 3033 Size: 591 846 1596 Fragments arranged by size: 1596 846 591 PspOMI G'GGCC_C Cuts at: 0 966 3033 Size: 966 2067 SacI G_AGCT'C Cuts at: 0 935 2317 3033 Size: 935 1382 716 Fragments arranged by size: 1382 935 716 SacII CC_GC'GG Cuts at: 0 1995 3033 Size: 1995 1038 SalI G'TCGA_C Cuts at: 0 3026 3033 Size: 3026 7 SfcI C'TryA_G Cuts at: 0 1744 2724 2889 3033 Size: 1744 980 165 144 Fragments arranged by size: 1744 980 165 144 SmlI C'TyrA_G Cuts at: 0 647 767 1335 1552 1855 2921 3033 Size: 647 120 568 217 303 1066 112 Fragments arranged by size: 1066 647 568 303 217 120 112 SpeI A'CTAG_T Cuts at: 0 1275 3033 Size: 1275 1758 SspI AAT'ATT Cuts at: 0 850 3033 Size: 850 2183 StyI C'CwwG_G Cuts at: 0 587 3033 Size: 587 2446 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 158 3033 Size: 158 2875 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1130 1162 2144 2176 3033 Size: 1130 32 982 32 857 Fragments arranged by size: 1130 982 857 32 32 XbaI T'CTAG_A Cuts at: 0 1536 3033 Size: 1536 1497 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 87 223 315 2433 3033 Size: 87 136 92 2118 600 Fragments arranged by size: 2118 600 136 92 87 XhoI C'TCGA_G Cuts at: 0 647 3033 Size: 647 2386 XmnI GAAnn'nnTTC Cuts at: 0 424 3033 Size: 424 2609 Enzymes that do cut: AarI AccI Acc65I AclI AcuI AflII AflIII AhdI AleI AlfI AlwNI ApaI ApoI AseI AvaI BamHI BanI BanII BbvCI BclI BglII BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspHI BspMI BssSI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI SacI SacII SalI SfcI SmlI SpeI SspI StyI TaqII TstI XbaI XcmI XhoI XmnI Enzymes that do not cut: AatII AfeI AgeI AloI ApaLI AscI AsiSI AvrII BaeI BbeI BbsI BcgI BciVI BfrBI BglI BmrI BmtI BplI BsaBI BsiEI BsiWI BsmI BsmBI BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstEII CspCI EagI EcoRV FalI FseI HpaI KasI MfeI MluI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI SwaI TatI Tth111I XmaI ZraI