(Circular) (Six-base) MAPSORT of: pFN21AE9656.seq Check: 4168 from: 1 to: 5197 pFN21AE9656 5197 bp With 182 enzymes: SgfI * March 10, 2017 10:57 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3007 278 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2206 2540 1937 Size: 269 334 4594 Fragments arranged by size: 4594 334 269 Acc65I G'GTAC_C Cuts at: 2518 2518 Size: 5197 AclI AA'CG_TT Cuts at: 1563 3324 3697 1563 Size: 1761 373 3063 Fragments arranged by size: 3063 1761 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2365 3260 4644 4912 1632 Size: 733 895 1384 268 1917 Fragments arranged by size: 1917 1384 895 733 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5000 AflIII A'CryG_T Cuts at: 1203 4096 5073 1203 Size: 2893 977 1327 Fragments arranged by size: 2893 1327 977 AgeI A'CCGG_T Cuts at: 4028 4028 Size: 5197 AhdI GACnn_n'nnGTC Cuts at: 3926 3926 Size: 5197 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5197 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5197 AlwNI CAG_nnn'CTG Cuts at: 2940 4512 2940 Size: 1572 3625 ApaI G_GGCC'C Cuts at: 2127 2171 2127 Size: 44 5153 ApaLI G'TGCA_C Cuts at: 3254 4410 3254 Size: 1156 4041 ApoI r'AATT_y Cuts at: 1493 2506 2732 2884 4993 1493 Size: 1013 226 152 2109 1697 Fragments arranged by size: 2109 1697 1013 226 152 AseI AT'TA_AT Cuts at: 160 1032 3751 160 Size: 872 2719 1606 Fragments arranged by size: 2719 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 AvaI C'yCGr_G Cuts at: 1870 1943 2171 2489 2522 1870 Size: 73 228 318 33 4545 Fragments arranged by size: 4545 318 228 73 33 BamHI G'GATC_C Cuts at: 2527 2894 2527 Size: 367 4830 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2037 2227 2485 2518 3973 Size: 325 217 627 250 190 258 33 1455 Cuts at: 3973 618 Size: 1842 Fragments arranged by size: 1842 1455 627 325 258 250 217 190 33 BanII G_rGCy'C Cuts at: 1391 2127 2171 2516 1391 Size: 736 44 345 4072 Fragments arranged by size: 4072 736 345 44 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4226 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3388 3422 1903 Size: 34 1451 34 3678 Fragments arranged by size: 3678 1451 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2240 3089 4299 2240 Size: 849 1210 3138 Fragments arranged by size: 3138 1210 849 BclI T'GATC_A Cuts at: 1548 1548 Size: 5197 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3808 136 Size: 107 122 71 631 605 2136 1525 Fragments arranged by size: 2136 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5192 5192 Size: 5197 BlpI GC'TnA_GC Cuts at: 2614 2614 Size: 5197 Bme1580I G_kGCm'C Cuts at: 1790 2127 2171 2490 3258 4414 1790 Size: 337 44 319 768 1156 2573 Fragments arranged by size: 2573 1156 768 337 319 44 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5197 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2928 3886 442 Size: 416 53 83 399 24 1511 958 1753 Fragments arranged by size: 1753 1511 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5197 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2426 3857 1239 Size: 1121 66 1431 2579 Fragments arranged by size: 2579 1431 1121 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3322 4187 4485 3322 Size: 865 298 4034 Fragments arranged by size: 4034 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2402 3860 882 Size: 617 903 1458 2219 Fragments arranged by size: 2219 1458 903 617 BsaAI yAC'GTr Cuts at: 493 1204 2251 2988 493 Size: 711 1047 737 2702 Fragments arranged by size: 2702 1047 737 711 BsaBI GATnn'nnATC Cuts at: 5191 5191 Size: 5197 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 3004 3386 4021 Size: 53 83 186 936 540 931 382 635 Cuts at: 4021 275 Size: 1451 Fragments arranged by size: 1451 936 931 635 540 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3630 4028 4302 4449 4860 3630 Size: 398 274 147 411 3967 Fragments arranged by size: 3967 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 2205 2235 1178 Size: 30 165 30 636 30 136 30 4140 Fragments arranged by size: 4140 636 165 136 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2342 2345 1186 Size: 1045 111 3 4038 Fragments arranged by size: 4038 1045 111 3 BseYI C'CCAG_C Cuts at: 2456 4400 4896 2456 Size: 1944 496 2757 Fragments arranged by size: 2757 1944 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4624 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2356 2647 3408 4436 1540 Size: 275 186 355 291 761 1028 2301 Fragments arranged by size: 2301 1028 761 355 291 275 186 BsiHKAI G_wGCw'C Cuts at: 3258 3343 4414 3258 Size: 85 1071 4041 Fragments arranged by size: 4041 1071 85 BsmI GAATG_Cn' Cuts at: 2708 2801 2708 Size: 93 5104 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2127 2171 2490 2516 3258 3343 4414 Size: 399 337 44 319 26 742 85 1071 Cuts at: 4414 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 337 319 85 44 26 BspEI T'CCGG_A Cuts at: 4860 4860 Size: 5197 BspHI T'CATG_A Cuts at: 3086 4816 3086 Size: 1730 3467 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2552 844 Size: 1708 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2013 2344 3084 1394 Size: 429 190 331 740 3507 Fragments arranged by size: 3507 740 429 331 190 BsrDI GCAATG_nn' Cuts at: 65 1248 2063 3692 3866 65 Size: 1183 815 1629 174 1396 Fragments arranged by size: 1629 1396 1183 815 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3841 4028 1700 Size: 110 2031 187 2869 Fragments arranged by size: 2869 2031 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5197 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5197 BssSI C'ACGA_G Cuts at: 2240 3257 4269 2240 Size: 1017 1012 3168 Fragments arranged by size: 3168 1017 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2098 794 Size: 1304 3893 BstBI TT'CG_AA Cuts at: 4001 4001 Size: 5197 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5197 BstYI r'GATC_y Cuts at: 1972 2527 2894 3279 3296 4748 4834 4846 5192 Size: 555 367 385 17 1452 86 12 346 Cuts at: 5192 1972 Size: 1977 Fragments arranged by size: 1977 1452 555 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3706 Fragments arranged by size: 3706 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4458 Fragments arranged by size: 4458 683 56 BtsI GCAGTG_nn' Cuts at: 2087 2370 2713 3507 3527 2087 Size: 283 343 794 20 3757 Fragments arranged by size: 3757 794 343 283 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5197 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2419 2454 529 Size: 35 1855 35 3272 Fragments arranged by size: 3272 1855 35 35 DraI TTT'AAA Cuts at: 2501 2501 Size: 5197 DraIII CAC_nnn'GTG Cuts at: 2397 2397 Size: 5197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4204 809 Size: 507 2888 1802 Fragments arranged by size: 2888 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2644 3533 Size: 54 1425 50 275 18 195 619 889 Cuts at: 3533 5162 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 619 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2644 1537 Size: 275 832 4090 Fragments arranged by size: 4090 832 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 3127 1353 Size: 906 868 3423 Fragments arranged by size: 3423 906 868 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2047 2150 3784 4158 4304 387 Size: 1064 138 458 103 1634 374 146 1280 Fragments arranged by size: 1634 1280 1064 458 374 146 138 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2365 2426 3260 3857 4644 4912 Size: 393 728 5 61 834 597 787 268 Cuts at: 4912 1239 Size: 1524 Fragments arranged by size: 1524 834 787 728 597 393 268 61 5 EcoO109I rG'GnC_Cy Cuts at: 2167 2168 2442 2167 Size: 1 274 4922 Fragments arranged by size: 4922 274 1 EcoRI G'AATT_C Cuts at: 2506 2884 2506 Size: 378 4819 FspI TGC'GCA Cuts at: 3703 3703 Size: 5197 HaeII r_GCGC'y Cuts at: 1678 2143 4344 1678 Size: 465 2201 2531 Fragments arranged by size: 2531 2201 465 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3838 3870 3912 3944 1373 Size: 32 135 32 2266 32 42 32 2626 Fragments arranged by size: 2626 2266 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2541 2787 669 Size: 988 281 603 246 3079 Fragments arranged by size: 3079 988 603 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5197 HpaI GTT'AAC Cuts at: 2787 2787 Size: 5197 KpnI G_GTAC'C Cuts at: 2522 2522 Size: 5197 MfeI C'AATT_G Cuts at: 2796 2796 Size: 5197 MluI A'CGCG_T Cuts at: 5073 5073 Size: 5197 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4311 4495 1305 Size: 3006 184 2007 Fragments arranged by size: 3006 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3375 Fragments arranged by size: 3375 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3155 3514 3673 518 Size: 648 1989 359 159 2042 Fragments arranged by size: 2042 1989 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2202 2338 2613 3290 4438 4683 1606 Size: 596 136 275 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 596 275 245 136 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3706 Fragments arranged by size: 3706 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5197 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5197 NotI GC'GGCC_GC Cuts at: 2644 2644 Size: 5197 NspI r_CATG'y Cuts at: 1132 1229 2555 4100 1132 Size: 97 1326 1545 2229 Fragments arranged by size: 2229 1545 1326 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5197 PciI A'CATG_T Cuts at: 4096 4096 Size: 5197 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5197 PfoI T'CCnGG_A Cuts at: 2309 2309 Size: 5197 PmeI GTTT'AAAC Cuts at: 2501 2501 Size: 5197 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3266 3298 4779 4811 3266 Size: 32 1481 32 3652 Fragments arranged by size: 3652 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5197 PsiI TTA'TAA Cuts at: 2767 2767 Size: 5197 PspOMI G'GGCC_C Cuts at: 2123 2167 2123 Size: 44 5153 PstI C_TGCA'G Cuts at: 830 2549 830 Size: 1719 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5197 SalI G'TCGA_C Cuts at: 1936 2539 1936 Size: 603 4594 SbfI CC_TGCA'GG Cuts at: 2549 2549 Size: 5197 ScaI AGT'ACT Cuts at: 3445 5157 3445 Size: 1712 3485 SfcI C'TryA_G Cuts at: 826 1045 2545 3680 4361 4552 826 Size: 219 1500 1135 681 191 1471 Fragments arranged by size: 1500 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5197 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5197 SmaI CCC'GGG Cuts at: 1872 2524 1872 Size: 652 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3301 4202 4464 820 Size: 197 926 1358 901 262 1553 Fragments arranged by size: 1553 1358 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2988 493 Size: 2495 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5197 SphI G_CATG'C Cuts at: 1132 2555 1132 Size: 1423 3774 SspI AAT'ATT Cuts at: 5 52 3121 5 Size: 47 3069 2081 Fragments arranged by size: 3069 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5197 StyI C'CwwG_G Cuts at: 513 1065 2004 2636 513 Size: 552 939 632 3074 Fragments arranged by size: 3074 939 632 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3224 3241 3394 3579 4024 325 Size: 1255 1644 17 153 185 445 1498 Fragments arranged by size: 1644 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3443 5155 96 Size: 274 80 33 51 1443 1466 1712 138 Fragments arranged by size: 1712 1466 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2039 2071 2169 2201 2205 2237 5157 5189 2039 Size: 32 98 32 4 32 2920 32 2047 Fragments arranged by size: 2920 2047 98 32 32 32 32 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5197 XbaI T'CTAG_A Cuts at: 2533 2533 Size: 5197 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5197 XmaI C'CCGG_G Cuts at: 1870 2522 1870 Size: 652 4545 XmnI GAAnn'nnTTC Cuts at: 3326 3326 Size: 5197 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3005 276 Size: 53 83 186 936 1471 2468 Fragments arranged by size: 2468 1471 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI