Restriction map for pFN21AE9656
 (Linear) (Six-base) MAPSORT of: KIEE9656.seq  Check: 3722  from: 1  to: 492



KIEE9656.orf

 With 182 enzymes: SgfI * 

                        March 10, 2017 10:38  ..

AccI GT'mk_AC

Cuts at:      0     201     492 
   Size:        201     291
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     360     492 
   Size:        360     132
 
ApaI G_GGCC'C

Cuts at:      0     122     166     492 
   Size:        122      44     326

  Fragments arranged by size: 

                 326     122      44
 
AvaI C'yCGr_G

Cuts at:      0     166     484     492 
   Size:        166     318       8

  Fragments arranged by size: 

                 318     166       8
 
BanI G'GyrC_C

Cuts at:      0      32     222     480     492 
   Size:         32     190     258      12

  Fragments arranged by size: 

                 258     190      32      12
 
BanII G_rGCy'C

Cuts at:      0     122     166     492 
   Size:        122      44     326

  Fragments arranged by size: 

                 326     122      44
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     235     492 
   Size:        235     257
 
Bme1580I G_kGCm'C

Cuts at:      0     122     166     485     492 
   Size:        122      44     319       7

  Fragments arranged by size: 

                 319     122      44       7
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     421     492 
   Size:        355      66      71

  Fragments arranged by size: 

                 355      71      66
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     397     492 
   Size:        397      95
 
BsaAI yAC'GTr

Cuts at:      0     246     492 
   Size:        246     246
 
BsaHI Gr'CG_yC

Cuts at:      0      68     492 
   Size:         68     424
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      34      64     200     230     492 
   Size:         34      30     136      30     262

  Fragments arranged by size: 

                 262     136      34      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     226     337     340     492 
   Size:        226     111       3     152

  Fragments arranged by size: 

                 226     152     111       3
 
BseYI C'CCAG_C

Cuts at:      0     451     492 
   Size:        451      41
 
BsiEI CG_ry'CG

Cuts at:      0     351     492 
   Size:        351     141
 
Bsp1286I G_dGCh'C

Cuts at:      0     122     166     485     492 
   Size:        122      44     319       7

  Fragments arranged by size: 

                 319     122      44       7
 
BsrBI CCG'CTC

Cuts at:      0       8     339     492 
   Size:          8     331     153

  Fragments arranged by size: 

                 331     153       8
 
BsrDI GCAATG_nn'

Cuts at:      0      58     492 
   Size:         58     434
 
BssSI C'ACGA_G

Cuts at:      0     235     492 
   Size:        235     257
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      93     492 
   Size:         93     399
 
BtsI GCAGTG_nn'

Cuts at:      0      82     365     492 
   Size:         82     283     127

  Fragments arranged by size: 

                 283     127      82
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     414     449     492 
   Size:        414      35      43

  Fragments arranged by size: 

                 414      43      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     392     492 
   Size:        392     100
 
EaeI y'GGCC_r

Cuts at:      0      20     492 
   Size:         20     472
 
EarI CTCTTCn'nnn_

Cuts at:      0     254     492 
   Size:        254     238
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      42     145     492 
   Size:         42     103     347

  Fragments arranged by size: 

                 347     103      42
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     355     360     421     492 
   Size:        355       5      61      71

  Fragments arranged by size: 

                 355      71      61       5
 
EcoO109I rG'GnC_Cy

Cuts at:      0     162     163     437     492 
   Size:        162       1     274      55

  Fragments arranged by size: 

                 274     162      55       1
 
HaeII r_GCGC'y

Cuts at:      0     138     492 
   Size:        138     354
 
MspA1I CmG'CkG

Cuts at:      0     197     333     492 
   Size:        197     136     159

  Fragments arranged by size: 

                 197     159     136
 
PfoI T'CCnGG_A

Cuts at:      0     304     492 
   Size:        304     188
 
PspOMI G'GGCC_C

Cuts at:      0     118     162     492 
   Size:        118      44     330

  Fragments arranged by size: 

                 330     118      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      34      66     164     196     200     232     492 
   Size:         34      32      98      32       4      32     260

  Fragments arranged by size: 

                 260      98      34      32      32      32       4
 

 Enzymes that do cut:

     AccI     AcuI     ApaI     AvaI     BanI    BanII    BciVI Bme1580I
     BpmI     BsaI    BsaAI    BsaHI    BsaXI    BseRI    BseYI    BsiEI
 Bsp1286I    BsrBI    BsrDI    BssSI   BstAPI     BtsI    CspCI   DraIII
     EaeI     EarI     EciI  Eco57MI EcoO109I    HaeII   MspA1I     PfoI
   PspOMI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI
     BmtI     BplI   Bpu10I    BpuEI    BsaBI    BsaWI     BsgI  BsiHKAI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrFI    BsrGI
   BssHII    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI
    BtgZI     ClaI     DraI     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI
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