################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BfaI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BseRI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstF5I BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I Eco81I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FokI FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KpnI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MseI MunI NcoI NdeI NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI TfiI Tru9I Tsp509I TspRI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 13 GGCGCC G[GA]^CG[CT]C AfaI 42 GTAC GT^AC AflIII 38 ACATGT A^C[GA][CT]GT Aor51HI 359 AGCGCT AGC^GCT AvaII 234 GGACC G^G[AT]CC BanI 13 GGCGCC G^G[CT][GA]CC BbeI 13 GGCGCC GGCGC^C BclI 32 TGATCA T^GATCA BpmI 160 CTGGAG CTGGAG(16/14) BsaHI 13 GGCGCC G[GA]^CG[CT]C BsaI 67 GGTCTC GGTCTC(1/5) BsmBI 62 GAGACG CGTCTC(1/5) BsmFI 641 GTCCC GGGAC(10/14) BspHI 338 TCATGA T^CATGA BspLU11I 38 ACATGT A^CATGT BspMI 44 ACCTGC ACCTGC(4/8) BsrBI 9 GAGCGG CCGCTC(-3/-3) BssHII 473 GCGCGC G^CGCGC EarI 70 CTCTTC CTCTTC(1/4) Eco47III 359 AGCGCT AGC^GCT EcoNI 390 CCTGGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 32 TGATCA T^GATCA Hin1I 13 GGCGCC G[GA]^CG[CT]C KasI 13 GGCGCC G^GCGCC Ksp632I 70 CTCTTC CTCTTC(1/4) MaeIII 212 GTCAC ^GT[ACGT]AC MslI 421 CACGCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 13 GGCGCC GG^CGCC NotI 457 GCGGCCGC GC^GGCCGC NspI 38 ACATGT [GA]CATG^[CT] PflMI 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 338 TCATGA T^CATGA RsaI 42 GTAC GT^AC SfaNI 194 GCATC GCATC(5/9) SfiI 543 GGCCGCCTCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 13 GGCGCC GGC^GCC StuI 398 AGGCCT AGG^CCT TaqI 377 TCGA T^CGA Tsp45I 212 GTCAC ^GT[GC]AC TthHB8I 377 TCGA T^CGA Van91I 384 CCAGGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 52 GATCC GGATC(4/5) 138 GATCC BglI 451 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 544 GCCGCCTCGGC BlpI 105 GCTGAGC GC^T[ACGT]AGC 300 GCTGAGC Bpu1102I 105 GCTGAGC GC^T[ACGT]AGC 300 GCTGAGC BsiEI 458 CGGCCG CG[GA][CT]^CG 551 CGGCCG BsrI 581 ACTGG ACTGG(1/-1) 638 CCAGT BstAPI 150 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 439 GCAGCTGCTGC BstYI 51 AGATCC [GA]^GATC[CT] 137 AGATCC CelII 105 GCTGAGC GC^T[ACGT]AGC 300 GCTGAGC DdeI 106 CTGAG C^T[ACGT]AG 301 CTGAG EagI 458 CGGCCG C^GGCCG 551 CGGCCG EclXI 458 CGGCCG C^GGCCG 551 CGGCCG Eco52I 458 CGGCCG C^GGCCG 551 CGGCCG HaeII 13 GGCGCC [GA]GCGC^[CT] 359 AGCGCT KspI 113 CCGCGG CCGC^GG 455 CCGCGG MboII 84 GAAGA GAAGA(8/7) 71 TCTTC MflI 51 AGATCC [GA]^GATC[CT] 137 AGATCC PvuII 142 CAGCTG CAG^CTG 440 CAGCTG SacII 113 CCGCGG CCGC^GG 455 CCGCGG SmaI 591 CCCGGG CCC^GGG 661 CCCGGG XhoII 51 AGATCC [GA]^GATC[CT] 137 AGATCC XmaCI 591 CCCGGG C^CCGGG 661 CCCGGG XmaI 591 CCCGGG C^CCGGG 661 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AluI AlwNI ApaI AvaI BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsoBI Bsp1286I BsrFI BssKI BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboI MnlI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV Sau3AI Sau96I ScrFI TseI