(Circular) (Six-base) MAPSORT of: pFN21AB7504.seq Check: 4975 from: 1 to: 5419 pFN21AB7504 5419 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3229 278 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2762 1937 Size: 825 4594 Acc65I G'GTAC_C Cuts at: 2740 2740 Size: 5419 AclI AA'CG_TT Cuts at: 1563 3546 3919 1563 Size: 1983 373 3063 Fragments arranged by size: 3063 1983 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3482 4866 5134 1632 Size: 1850 1384 268 1917 Fragments arranged by size: 1917 1850 1384 268 AfeI AGC'GCT Cuts at: 2366 2366 Size: 5419 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5222 AflIII A'CryG_T Cuts at: 1203 2043 4318 5295 1203 Size: 840 2275 977 1327 Fragments arranged by size: 2275 1327 977 840 AgeI A'CCGG_T Cuts at: 4250 4250 Size: 5419 AhdI GACnn_n'nnGTC Cuts at: 4148 4148 Size: 5419 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5419 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2448 1184 Size: 1264 4155 AlwNI CAG_nnn'CTG Cuts at: 2032 2161 2395 2450 3162 4734 2032 Size: 129 234 55 712 1572 2717 Fragments arranged by size: 2717 1572 712 234 129 55 ApaI G_GGCC'C Cuts at: 2597 2603 2673 2597 Size: 6 70 5343 Fragments arranged by size: 5343 70 6 ApaLI G'TGCA_C Cuts at: 3476 4632 3476 Size: 1156 4263 ApoI r'AATT_y Cuts at: 1493 2728 2954 3106 5215 1493 Size: 1235 226 152 2109 1697 Fragments arranged by size: 2109 1697 1235 226 152 AseI AT'TA_AT Cuts at: 160 1032 3973 160 Size: 872 2941 1606 Fragments arranged by size: 2941 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2596 2666 2744 1870 Size: 73 152 501 70 78 4545 Fragments arranged by size: 4545 501 152 78 73 70 BamHI G'GATC_C Cuts at: 2749 3116 2749 Size: 367 5052 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2018 2740 4195 618 Size: 325 217 627 231 722 1455 1842 Fragments arranged by size: 1842 1455 722 627 325 231 217 BanII G_rGCy'C Cuts at: 1391 2117 2597 2603 2673 2738 1391 Size: 726 480 6 70 65 4072 Fragments arranged by size: 4072 726 480 70 65 6 BbeI G_GCGC'C Cuts at: 2022 2022 Size: 5419 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4448 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3610 3644 1903 Size: 34 1673 34 3678 Fragments arranged by size: 3678 1673 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3311 4521 3311 Size: 1210 4209 BclI T'GATC_A Cuts at: 1548 2037 1548 Size: 489 4930 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2462 2555 4030 Size: 107 122 71 631 605 790 93 1475 Cuts at: 4030 136 Size: 1525 Fragments arranged by size: 1525 1475 790 631 605 122 107 93 71 BglII A'GATC_T Cuts at: 5414 5414 Size: 5419 BlpI GC'TnA_GC Cuts at: 2111 2306 2836 2111 Size: 195 530 4694 Fragments arranged by size: 4694 530 195 Bme1580I G_kGCm'C Cuts at: 1790 2597 2603 2673 3480 4636 1790 Size: 807 6 70 807 1156 2573 Fragments arranged by size: 2573 1156 807 807 70 6 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5419 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2637 3150 4108 Size: 416 53 83 399 24 1220 513 958 Cuts at: 4108 442 Size: 1753 Fragments arranged by size: 1753 1220 958 513 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5419 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2090 2090 Size: 5419 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2186 4079 1239 Size: 947 1893 2579 Fragments arranged by size: 2579 1893 947 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3544 4409 4707 3544 Size: 865 298 4256 Fragments arranged by size: 4256 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2078 4082 882 Size: 617 579 2004 2219 Fragments arranged by size: 2219 2004 617 579 BsaAI yAC'GTr Cuts at: 493 1204 3210 493 Size: 711 2006 2702 Fragments arranged by size: 2702 2006 711 BsaBI GATnn'nnATC Cuts at: 5413 5413 Size: 5419 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2019 3226 3608 4243 Size: 53 83 186 936 486 1207 382 635 Cuts at: 4243 275 Size: 1451 Fragments arranged by size: 1451 1207 936 635 486 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3852 4250 4524 4671 5082 3852 Size: 398 274 147 411 4189 Fragments arranged by size: 4189 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5194 Fragments arranged by size: 5194 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5419 BseYI C'CCAG_C Cuts at: 2025 2100 2648 4622 5118 2025 Size: 75 548 1974 496 2326 Fragments arranged by size: 2326 1974 548 496 75 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4846 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2466 2559 2869 3630 4658 1540 Size: 275 186 465 93 310 761 1028 2301 Fragments arranged by size: 2301 1028 761 465 310 275 186 93 BsiHKAI G_wGCw'C Cuts at: 3480 3565 4636 3480 Size: 85 1071 4263 Fragments arranged by size: 4263 1071 85 BsmI GAATG_Cn' Cuts at: 2930 3023 2930 Size: 93 5326 BsmBI CGTCTCn'nnnn_ Cuts at: 2061 2061 Size: 5419 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2597 2603 2673 2738 3480 3565 Size: 399 327 480 6 70 65 742 85 Cuts at: 3565 4636 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 480 399 327 85 70 65 6 BspEI T'CCGG_A Cuts at: 5082 5082 Size: 5419 BspHI T'CATG_A Cuts at: 2343 3308 5038 2343 Size: 965 1730 2724 Fragments arranged by size: 2724 1730 965 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2058 2774 844 Size: 1214 716 3489 Fragments arranged by size: 3489 1214 716 BsrBI CCG'CTC Cuts at: 1394 1823 2016 3306 1394 Size: 429 193 1290 3507 Fragments arranged by size: 3507 1290 429 193 BsrDI GCAATG_nn' Cuts at: 65 1248 3914 4088 65 Size: 1183 2666 174 1396 Fragments arranged by size: 2666 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2192 2498 2573 4063 4250 1700 Size: 110 382 306 75 1490 187 2869 Fragments arranged by size: 2869 1490 382 306 187 110 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5419 BssHII G'CGCG_C Cuts at: 1925 2478 1925 Size: 553 4866 BssSI C'ACGA_G Cuts at: 3479 4491 3479 Size: 1012 4407 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 2450 794 Size: 1367 289 3763 Fragments arranged by size: 3763 1367 289 BstBI TT'CG_AA Cuts at: 4223 4223 Size: 5419 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5419 BstYI r'GATC_y Cuts at: 1972 2056 2142 2749 3116 3501 3518 4970 5056 Size: 84 86 607 367 385 17 1452 86 Cuts at: 5056 5068 5414 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 607 385 367 346 86 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 2460 2682 513 Size: 552 1053 342 222 3250 Fragments arranged by size: 3250 1053 552 342 222 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2185 487 Size: 683 56 959 3721 Fragments arranged by size: 3721 959 683 56 BtsI GCAGTG_nn' Cuts at: 2935 3729 3749 2935 Size: 794 20 4605 Fragments arranged by size: 4605 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5419 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5384 DraI TTT'AAA Cuts at: 2723 2723 Size: 5419 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4426 809 Size: 507 3110 1802 Fragments arranged by size: 3110 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2463 2547 2556 Size: 54 1425 50 275 18 633 84 9 Cuts at: 2556 2588 2685 2866 3755 5384 8 Size: 32 97 181 889 1629 43 Fragments arranged by size: 1629 1425 889 633 275 181 97 84 54 50 43 32 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2463 2556 2866 1537 Size: 275 651 93 310 4090 Fragments arranged by size: 4090 651 310 275 93 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 3349 1353 Size: 728 1268 3423 Fragments arranged by size: 3423 1268 728 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4006 4380 4526 387 Size: 1064 138 2417 374 146 1280 Fragments arranged by size: 2417 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2186 3482 4079 4866 5134 1239 Size: 393 554 1296 597 787 268 1524 Fragments arranged by size: 1524 1296 787 597 554 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2399 2399 Size: 5419 EcoO109I rG'GnC_Cy Cuts at: 2392 2600 2670 2392 Size: 208 70 5141 Fragments arranged by size: 5141 208 70 EcoRI G'AATT_C Cuts at: 2728 3106 2728 Size: 378 5041 FspI TGC'GCA Cuts at: 3925 3925 Size: 5419 HaeII r_GCGC'y Cuts at: 1678 2022 2368 4566 1678 Size: 344 346 2198 2531 Fragments arranged by size: 2531 2198 346 344 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4060 4092 4134 4166 1373 Size: 32 135 32 2488 32 42 32 2626 Fragments arranged by size: 2626 2488 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2763 3009 669 Size: 988 281 825 246 3079 Fragments arranged by size: 3079 988 825 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5419 HpaI GTT'AAC Cuts at: 3009 3009 Size: 5419 KasI G'GCGC_C Cuts at: 2018 2018 Size: 5419 KpnI G_GTAC'C Cuts at: 2744 2744 Size: 5419 MfeI C'AATT_G Cuts at: 3018 3018 Size: 5419 MluI A'CGCG_T Cuts at: 5295 5295 Size: 5419 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2044 4533 4717 1305 Size: 739 2489 184 2007 Fragments arranged by size: 2489 2007 739 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3597 Fragments arranged by size: 3597 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2430 3377 3736 3895 518 Size: 648 1264 947 359 159 2042 Fragments arranged by size: 2042 1264 947 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2110 2120 2136 2149 2447 2462 2512 2615 Size: 504 10 16 13 298 15 50 103 Cuts at: 2615 2835 3512 4660 4905 1606 Size: 220 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 504 298 245 220 103 50 16 15 13 10 NaeI GCC'GGC Cuts at: 2194 2500 2575 2194 Size: 306 75 5038 Fragments arranged by size: 5038 306 75 NarI GG'CG_CC Cuts at: 2019 2019 Size: 5419 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4867 NdeI CA'TA_TG Cuts at: 387 387 Size: 5419 NgoMIV G'CCGG_C Cuts at: 2192 2498 2573 2192 Size: 306 75 5038 Fragments arranged by size: 5038 306 75 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5419 NotI GC'GGCC_GC Cuts at: 2463 2866 2463 Size: 403 5016 NspI r_CATG'y Cuts at: 1132 1229 2047 2777 4322 1132 Size: 97 818 730 1545 2229 Fragments arranged by size: 2229 1545 818 730 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5419 PciI A'CATG_T Cuts at: 2043 4318 2043 Size: 2275 3144 PflMI CCAn_nnn'nTGG Cuts at: 1108 2395 1108 Size: 1287 4132 PmeI GTTT'AAAC Cuts at: 2723 2723 Size: 5419 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3488 3520 5001 5033 3488 Size: 32 1481 32 3874 Fragments arranged by size: 3874 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5419 PsiI TTA'TAA Cuts at: 2989 2989 Size: 5419 PspOMI G'GGCC_C Cuts at: 2593 2599 2669 2593 Size: 6 70 5343 Fragments arranged by size: 5343 70 6 PstI C_TGCA'G Cuts at: 830 2771 830 Size: 1941 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5419 PvuII CAG'CTG Cuts at: 2149 2447 2149 Size: 298 5121 SacII CC_GC'GG Cuts at: 2121 2463 2121 Size: 342 5077 SalI G'TCGA_C Cuts at: 1936 2761 1936 Size: 825 4594 SbfI CC_TGCA'GG Cuts at: 2771 2771 Size: 5419 ScaI AGT'ACT Cuts at: 3667 5379 3667 Size: 1712 3707 SfcI C'TryA_G Cuts at: 826 1045 2767 3902 4583 4774 826 Size: 219 1722 1135 681 191 1471 Fragments arranged by size: 1722 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2555 2555 Size: 5419 SfoI GGC'GCC Cuts at: 2020 2020 Size: 5419 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5419 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5419 SmaI CCC'GGG Cuts at: 1872 2598 2668 2746 1872 Size: 726 70 78 4545 Fragments arranged by size: 4545 726 78 70 SmlI C'TyrA_G Cuts at: 820 1017 1943 3523 4424 4686 820 Size: 197 926 1580 901 262 1553 Fragments arranged by size: 1580 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3210 493 Size: 2717 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5419 SphI G_CATG'C Cuts at: 1132 2777 1132 Size: 1645 3774 SrfI GCCC'GGGC Cuts at: 2598 2668 2598 Size: 70 5349 SspI AAT'ATT Cuts at: 5 52 3343 5 Size: 47 3291 2081 Fragments arranged by size: 3291 2081 47 StuI AGG'CCT Cuts at: 1517 2405 1517 Size: 888 4531 StyI C'CwwG_G Cuts at: 513 1065 2858 513 Size: 552 1793 3074 Fragments arranged by size: 3074 1793 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3446 3463 3616 3801 4246 325 Size: 1255 1866 17 153 185 445 1498 Fragments arranged by size: 1866 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3665 5377 96 Size: 274 80 33 51 1443 1688 1712 138 Fragments arranged by size: 1712 1688 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5379 5411 5379 Size: 32 5387 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5419 XbaI T'CTAG_A Cuts at: 2755 2755 Size: 5419 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5419 XmaI C'CCGG_G Cuts at: 1870 2596 2666 2744 1870 Size: 726 70 78 4545 Fragments arranged by size: 4545 726 78 70 XmnI GAAnn'nnTTC Cuts at: 3548 3548 Size: 5419 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3227 276 Size: 53 83 186 936 1693 2468 Fragments arranged by size: 2468 1693 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacI SanDI SapI SexAI SwaI XcmI