(Circular) (Six-base) MAPSORT of: pFN21AE0957.seq Check: 5294 from: 1 to: 6184 pFN21AE0957 6184 bp With 182 enzymes: SgfI * August 16, 2010 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2167 2167 Size: 6184 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3994 278 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2419 2551 3527 1937 Size: 482 132 976 4594 Fragments arranged by size: 4594 976 482 132 Acc65I G'GTAC_C Cuts at: 3505 3505 Size: 6184 AclI AA'CG_TT Cuts at: 1563 4311 4684 1563 Size: 2748 373 3063 Fragments arranged by size: 3063 2748 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2102 2579 3258 4247 5631 5899 1632 Size: 470 477 679 989 1384 268 1917 Fragments arranged by size: 1917 1384 989 679 477 470 268 AfeI AGC'GCT Cuts at: 2028 2028 Size: 6184 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5987 AflIII A'CryG_T Cuts at: 1203 5083 6060 1203 Size: 3880 977 1327 Fragments arranged by size: 3880 1327 977 AgeI A'CCGG_T Cuts at: 5015 5015 Size: 6184 AhdI GACnn_n'nnGTC Cuts at: 4913 4913 Size: 6184 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6184 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6184 AlwNI CAG_nnn'CTG Cuts at: 3927 5499 3927 Size: 1572 4612 ApaLI G'TGCA_C Cuts at: 4241 5397 4241 Size: 1156 5028 ApoI r'AATT_y Cuts at: 1493 2715 3493 3719 3871 5980 1493 Size: 1222 778 226 152 2109 1697 Fragments arranged by size: 2109 1697 1222 778 226 152 AseI AT'TA_AT Cuts at: 160 1032 4738 160 Size: 872 3706 1606 Fragments arranged by size: 3706 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 AvaI C'yCGr_G Cuts at: 1870 1943 2010 3509 1870 Size: 73 67 1499 4545 Fragments arranged by size: 4545 1499 73 67 BamHI G'GATC_C Cuts at: 3514 3881 3514 Size: 367 5817 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3268 3505 4960 618 Size: 325 217 627 1481 237 1455 1842 Fragments arranged by size: 1842 1481 1455 627 325 237 217 BanII G_rGCy'C Cuts at: 1391 2070 2834 3503 1391 Size: 679 764 669 4072 Fragments arranged by size: 4072 764 679 669 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2215 3449 3477 928 Size: 971 316 1234 28 3635 Fragments arranged by size: 3635 1234 971 316 28 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4375 4409 1903 Size: 34 2438 34 3678 Fragments arranged by size: 3678 2438 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3344 4076 5286 3344 Size: 732 1210 4242 Fragments arranged by size: 4242 1210 732 BclI T'GATC_A Cuts at: 1548 2181 2880 1548 Size: 633 699 4852 Fragments arranged by size: 4852 699 633 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4795 136 Size: 107 122 71 631 605 3123 1525 Fragments arranged by size: 3123 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2509 6179 2509 Size: 3670 2514 BlpI GC'TnA_GC Cuts at: 2023 3601 2023 Size: 1578 4606 Bme1580I G_kGCm'C Cuts at: 1790 4245 5401 1790 Size: 2455 1156 2573 Fragments arranged by size: 2573 2455 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6184 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3208 3915 4873 Size: 416 53 83 399 24 1791 707 958 Cuts at: 4873 442 Size: 1753 Fragments arranged by size: 1791 1753 958 707 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6184 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2665 4844 1239 Size: 1426 2179 2579 Fragments arranged by size: 2579 2179 1426 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2202 4309 5174 5472 2202 Size: 2107 865 298 2914 Fragments arranged by size: 2914 2107 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2440 4847 882 Size: 617 941 2407 2219 Fragments arranged by size: 2407 2219 941 617 BsaAI yAC'GTr Cuts at: 493 1204 3975 493 Size: 711 2771 2702 Fragments arranged by size: 2771 2702 711 BsaBI GATnn'nnATC Cuts at: 6178 6178 Size: 6184 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3991 4373 5008 275 Size: 53 83 186 936 2458 382 635 1451 Fragments arranged by size: 2458 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4617 5015 5289 5436 5847 4617 Size: 398 274 147 411 4954 Fragments arranged by size: 4954 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3219 3249 1178 Size: 30 165 30 1816 30 4113 Fragments arranged by size: 4113 1816 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2668 3254 1186 Size: 1482 586 4116 Fragments arranged by size: 4116 1482 586 BseYI C'CCAG_C Cuts at: 5387 5883 5387 Size: 496 5688 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2279 1163 Size: 573 543 5068 Fragments arranged by size: 5068 573 543 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2061 3634 4395 5423 1540 Size: 275 186 60 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 60 BsiHKAI G_wGCw'C Cuts at: 2070 2429 4245 4330 5401 2070 Size: 359 1816 85 1071 2853 Fragments arranged by size: 2853 1816 1071 359 85 BsmI GAATG_Cn' Cuts at: 2218 2264 3695 3788 2218 Size: 46 1431 93 4614 Fragments arranged by size: 4614 1431 93 46 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2429 2834 3503 4245 4330 5401 Size: 399 280 359 405 669 742 85 1071 Cuts at: 5401 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 669 405 399 359 280 85 BspEI T'CCGG_A Cuts at: 5847 5847 Size: 6184 BspHI T'CATG_A Cuts at: 2821 4073 5803 2821 Size: 1252 1730 3202 Fragments arranged by size: 3202 1730 1252 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2129 2167 2962 3539 844 Size: 1285 38 795 577 3489 Fragments arranged by size: 3489 1285 795 577 38 BsrBI CCG'CTC Cuts at: 1394 1823 3413 4071 1394 Size: 429 1590 658 3507 Fragments arranged by size: 3507 1590 658 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2760 4679 4853 65 Size: 1183 1512 1919 174 1396 Fragments arranged by size: 1919 1512 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4828 5015 1700 Size: 110 3018 187 2869 Fragments arranged by size: 3018 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6184 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6184 BssSI C'ACGA_G Cuts at: 4244 5256 4244 Size: 1012 5172 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6184 BstBI TT'CG_AA Cuts at: 4988 4988 Size: 6184 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6184 BstXI CCAn_nnnn'nTGG Cuts at: 2344 3345 2344 Size: 1001 5183 BstYI r'GATC_y Cuts at: 1972 2509 2537 3277 3514 3881 4266 4283 5735 Size: 537 28 740 237 367 385 17 1452 Cuts at: 5735 5821 5833 6179 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 740 537 385 367 346 237 86 28 17 12 BtgI C'CryG_G Cuts at: 513 1065 2337 2868 513 Size: 552 1272 531 3829 Fragments arranged by size: 3829 1272 552 531 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2405 487 Size: 683 56 1179 4266 Fragments arranged by size: 4266 1179 683 56 BtsI GCAGTG_nn' Cuts at: 2291 3700 4494 4514 2291 Size: 1409 794 20 3961 Fragments arranged by size: 3961 1409 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6184 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6149 DraI TTT'AAA Cuts at: 3488 3488 Size: 6184 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5191 809 Size: 507 3875 1802 Fragments arranged by size: 3875 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2058 2346 3631 Size: 54 1425 50 275 18 228 288 1285 Cuts at: 3631 4520 6149 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1285 889 288 275 228 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2058 3631 1537 Size: 275 246 1573 4090 Fragments arranged by size: 4090 1573 275 246 EarI CTCTTCn'nnn_ Cuts at: 1353 3435 4114 1353 Size: 2082 679 3423 Fragments arranged by size: 3423 2082 679 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2021 4771 5145 5291 387 Size: 1064 138 432 2750 374 146 1280 Fragments arranged by size: 2750 1280 1064 432 374 146 138 EcoICRI GAG'CTC Cuts at: 2068 2068 Size: 6184 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2579 2665 3258 4247 4844 5631 Size: 393 470 477 86 593 989 597 787 Cuts at: 5631 5899 1239 Size: 268 1524 Fragments arranged by size: 1524 989 787 597 593 477 470 393 268 86 EcoRI G'AATT_C Cuts at: 3493 3871 3493 Size: 378 5806 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3007 3007 Size: 6184 FspI TGC'GCA Cuts at: 4690 4690 Size: 6184 HaeII r_GCGC'y Cuts at: 1678 2018 2030 2894 5331 1678 Size: 340 12 864 2437 2531 Fragments arranged by size: 2531 2437 864 340 12 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2963 2995 3159 3191 3318 Size: 32 135 32 1391 32 164 32 127 Cuts at: 3318 3350 3436 3468 4825 4857 4899 4931 1373 Size: 32 86 32 1357 32 42 32 2626 Fragments arranged by size: 2626 1391 1357 164 135 127 86 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2420 3528 3774 669 Size: 988 281 482 1108 246 3079 Fragments arranged by size: 3079 1108 988 482 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6184 HpaI GTT'AAC Cuts at: 3774 3774 Size: 6184 KpnI G_GTAC'C Cuts at: 3509 3509 Size: 6184 MfeI C'AATT_G Cuts at: 2574 2898 3248 3783 2574 Size: 324 350 535 4975 Fragments arranged by size: 4975 535 350 324 MluI A'CGCG_T Cuts at: 6060 6060 Size: 6184 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2262 2327 3205 5298 5482 1305 Size: 957 65 878 2093 184 2007 Fragments arranged by size: 2093 2007 957 878 184 65 MscI TGG'CCA Cuts at: 10 64 1489 1832 2348 10 Size: 54 1425 343 516 3846 Fragments arranged by size: 3846 1425 516 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4142 4501 4660 518 Size: 648 2976 359 159 2042 Fragments arranged by size: 2976 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2057 3600 4277 5425 5670 1606 Size: 451 1543 677 1148 245 2120 Fragments arranged by size: 2120 1543 1148 677 451 245 NcoI C'CATG_G Cuts at: 513 1065 2337 2868 513 Size: 552 1272 531 3829 Fragments arranged by size: 3829 1272 552 531 NdeI CA'TA_TG Cuts at: 387 2925 387 Size: 2538 3646 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6184 NotI GC'GGCC_GC Cuts at: 2058 3631 2058 Size: 1573 4611 NspI r_CATG'y Cuts at: 1132 1229 2142 2412 3542 5087 1132 Size: 97 913 270 1130 1545 2229 Fragments arranged by size: 2229 1545 1130 913 270 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6184 PciI A'CATG_T Cuts at: 5083 5083 Size: 6184 PflMI CCAn_nnn'nTGG Cuts at: 1108 2330 1108 Size: 1222 4962 PmeI GTTT'AAAC Cuts at: 3488 3488 Size: 6184 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2779 2811 4253 4285 5766 5798 2779 Size: 32 1442 32 1481 32 3165 Fragments arranged by size: 3165 1481 1442 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6184 PsiI TTA'TAA Cuts at: 3754 3754 Size: 6184 PstI C_TGCA'G Cuts at: 830 3536 830 Size: 2706 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6184 SacI G_AGCT'C Cuts at: 2070 2070 Size: 6184 SalI G'TCGA_C Cuts at: 1936 2418 3526 1936 Size: 482 1108 4594 Fragments arranged by size: 4594 1108 482 SapI GCTCTTCn'nnn_ Cuts at: 3435 3435 Size: 6184 SbfI CC_TGCA'GG Cuts at: 3536 3536 Size: 6184 ScaI AGT'ACT Cuts at: 4432 6144 4432 Size: 1712 4472 SfcI C'TryA_G Cuts at: 826 1045 2431 3532 4667 5348 5539 826 Size: 219 1386 1101 1135 681 191 1471 Fragments arranged by size: 1471 1386 1135 1101 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6184 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6184 SmaI CCC'GGG Cuts at: 1872 2012 3511 1872 Size: 140 1499 4545 Fragments arranged by size: 4545 1499 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 2217 4288 5189 5451 820 Size: 197 926 274 2071 901 262 1553 Fragments arranged by size: 2071 1553 926 901 274 262 197 SnaBI TAC'GTA Cuts at: 493 3975 493 Size: 3482 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6184 SphI G_CATG'C Cuts at: 1132 2412 3542 1132 Size: 1280 1130 3774 Fragments arranged by size: 3774 1280 1130 SspI AAT'ATT Cuts at: 5 52 2673 4108 5 Size: 47 2621 1435 2081 Fragments arranged by size: 2621 2081 1435 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6184 StyI C'CwwG_G Cuts at: 513 1065 2337 2752 2868 3623 513 Size: 552 1272 415 116 755 3074 Fragments arranged by size: 3074 1272 755 552 415 116 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4211 4228 4381 4566 5011 325 Size: 1255 2631 17 153 185 445 1498 Fragments arranged by size: 2631 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4430 6142 96 Size: 274 80 33 51 1443 2453 1712 138 Fragments arranged by size: 2453 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6144 6176 6144 Size: 32 6152 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6184 XbaI T'CTAG_A Cuts at: 3520 3520 Size: 6184 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3106 3106 Size: 6184 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6184 XmaI C'CCGG_G Cuts at: 1870 2010 3509 1870 Size: 140 1499 4545 Fragments arranged by size: 4545 1499 140 XmnI GAAnn'nnTTC Cuts at: 2672 4313 2672 Size: 1641 4543 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3992 276 Size: 53 83 186 936 2458 2468 Fragments arranged by size: 2468 2458 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI