(Linear) (Six-base) MAPSORT of: KIEE9533.seq Check: 1802 from: 1 to: 1230 KIEE9533.orf With 182 enzymes: SgfI * December 27, 2011 16:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 704 1230 Size: 704 526 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 1077 1181 1230 Size: 193 884 104 49 Fragments arranged by size: 884 193 104 49 AfeI AGC'GCT Cuts at: 0 397 1230 Size: 397 833 AflIII A'CryG_T Cuts at: 0 977 1230 Size: 977 253 AlwNI CAG_nnn'CTG Cuts at: 0 420 1230 Size: 420 810 ApaLI G'TGCA_C Cuts at: 0 160 340 579 1230 Size: 160 180 239 651 Fragments arranged by size: 651 239 180 160 AvaI C'yCGr_G Cuts at: 0 42 538 735 1230 Size: 42 496 197 495 Fragments arranged by size: 496 495 197 42 BamHI G'GATC_C Cuts at: 0 863 1209 1230 Size: 863 346 21 Fragments arranged by size: 863 346 21 BanI G'GyrC_C Cuts at: 0 197 1230 Size: 197 1033 BanII G_rGCy'C Cuts at: 0 272 566 1230 Size: 272 294 664 Fragments arranged by size: 664 294 272 BbeI G_GCGC'C Cuts at: 0 201 1230 Size: 201 1029 BbsI GAAGACnn'nnnn_ Cuts at: 0 297 814 1104 1230 Size: 297 517 290 126 Fragments arranged by size: 517 297 290 126 BbvCI CC'TCA_GC Cuts at: 0 787 1230 Size: 787 443 BciVI GTATCCnnnnn_n' Cuts at: 0 1182 1230 Size: 1182 48 BglI GCCn_nnn'nGGC Cuts at: 0 27 1230 Size: 27 1203 BlpI GC'TnA_GC Cuts at: 0 49 217 370 1050 1230 Size: 49 168 153 680 180 Fragments arranged by size: 680 180 168 153 49 Bme1580I G_kGCm'C Cuts at: 0 164 344 429 583 1230 Size: 164 180 85 154 647 Fragments arranged by size: 647 180 164 154 85 BmgBI CAC'GTC Cuts at: 0 855 1230 Size: 855 375 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 585 1230 Size: 585 645 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 781 859 1230 Size: 781 78 371 Fragments arranged by size: 781 371 78 Bpu10I CC'TnA_GC Cuts at: 0 385 787 1230 Size: 385 402 443 Fragments arranged by size: 443 402 385 BsaI GGTCTCn'nnnn_ Cuts at: 0 787 1230 Size: 787 443 BsaAI yAC'GTr Cuts at: 0 165 213 966 1230 Size: 165 48 753 264 Fragments arranged by size: 753 264 165 48 BsaHI Gr'CG_yC Cuts at: 0 59 198 442 1230 Size: 59 139 244 788 Fragments arranged by size: 788 244 139 59 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 815 845 1230 Size: 815 30 385 Fragments arranged by size: 815 385 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 775 790 1052 1077 1230 Size: 775 15 262 25 153 Fragments arranged by size: 775 262 153 25 15 BseYI C'CCAG_C Cuts at: 0 31 66 132 751 1230 Size: 31 35 66 619 479 Fragments arranged by size: 619 479 66 35 31 BsiHKAI G_wGCw'C Cuts at: 0 164 218 272 344 583 650 1139 1230 Size: 164 54 54 72 239 67 489 91 Fragments arranged by size: 489 239 164 91 72 67 54 54 BsmBI CGTCTCn'nnnn_ Cuts at: 0 535 1230 Size: 535 695 Bsp1286I G_dGCh'C Cuts at: 0 164 218 272 344 429 566 583 650 Size: 164 54 54 72 85 137 17 67 Cuts at: 650 1139 1230 Size: 489 91 Fragments arranged by size: 489 164 137 91 85 72 67 54 54 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 704 1230 Size: 704 526 BsrBI CCG'CTC Cuts at: 0 193 1230 Size: 193 1037 BsrDI GCAATG_nn' Cuts at: 0 153 1230 Size: 153 1077 BsrFI r'CCGG_y Cuts at: 0 261 1230 Size: 261 969 BsrGI T'GTAC_A Cuts at: 0 995 1230 Size: 995 235 BssSI C'ACGA_G Cuts at: 0 343 474 1230 Size: 343 131 756 Fragments arranged by size: 756 343 131 BstAPI GCAn_nnn'nTGC Cuts at: 0 168 664 1230 Size: 168 496 566 Fragments arranged by size: 566 496 168 BstEII G'GTnAC_C Cuts at: 0 1023 1154 1230 Size: 1023 131 76 Fragments arranged by size: 1023 131 76 BstXI CCAn_nnnn'nTGG Cuts at: 0 507 759 1185 1230 Size: 507 252 426 45 Fragments arranged by size: 507 426 252 45 BstYI r'GATC_y Cuts at: 0 863 1209 1230 Size: 863 346 21 Fragments arranged by size: 863 346 21 BtgI C'CryG_G Cuts at: 0 16 25 324 362 506 1230 Size: 16 9 299 38 144 724 Fragments arranged by size: 724 299 144 38 16 9 DraIII CAC_nnn'GTG Cuts at: 0 168 213 1230 Size: 168 45 1017 Fragments arranged by size: 1017 168 45 EaeI y'GGCC_r Cuts at: 0 509 1230 Size: 509 721 EarI CTCTTCn'nnn_ Cuts at: 0 1070 1108 1230 Size: 1070 38 122 Fragments arranged by size: 1070 122 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 562 1230 Size: 562 668 EcoICRI GAG'CTC Cuts at: 0 270 1230 Size: 270 960 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 193 781 859 1077 1181 1230 Size: 193 588 78 218 104 49 Fragments arranged by size: 588 218 193 104 78 49 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 828 1230 Size: 828 402 HaeII r_GCGC'y Cuts at: 0 133 201 229 392 399 472 1230 Size: 133 68 28 163 7 73 758 Fragments arranged by size: 758 163 133 73 68 28 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 815 847 1230 Size: 815 32 383 Fragments arranged by size: 815 383 32 KasI G'GCGC_C Cuts at: 0 197 1230 Size: 197 1033 MluI A'CGCG_T Cuts at: 0 977 1230 Size: 977 253 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 62 1230 Size: 62 1168 MslI CAynn'nnrTG Cuts at: 0 505 1183 1230 Size: 505 678 47 Fragments arranged by size: 678 505 47 MspA1I CmG'CkG Cuts at: 0 18 27 326 1054 1230 Size: 18 9 299 728 176 Fragments arranged by size: 728 299 176 18 9 NarI GG'CG_CC Cuts at: 0 198 1230 Size: 198 1032 NcoI C'CATG_G Cuts at: 0 506 1230 Size: 506 724 NruI TCG'CGA Cuts at: 0 573 1230 Size: 573 657 PasI CC'CwG_GG Cuts at: 0 299 1230 Size: 299 931 PflMI CCAn_nnn'nTGG Cuts at: 0 699 1230 Size: 699 531 PfoI T'CCnGG_A Cuts at: 0 1106 1230 Size: 1106 124 PmlI CAC'GTG Cuts at: 0 165 213 1230 Size: 165 48 1017 Fragments arranged by size: 1017 165 48 PvuII CAG'CTG Cuts at: 0 1054 1230 Size: 1054 176 SacI G_AGCT'C Cuts at: 0 272 1230 Size: 272 958 SacII CC_GC'GG Cuts at: 0 19 28 327 1230 Size: 19 9 299 903 Fragments arranged by size: 903 299 19 9 SfcI C'TryA_G Cuts at: 0 273 1230 Size: 273 957 SfiI GGCCn_nnn'nGGCC Cuts at: 0 27 1230 Size: 27 1203 SfoI GGC'GCC Cuts at: 0 199 1230 Size: 199 1031 SmaI CCC'GGG Cuts at: 0 44 737 1230 Size: 44 693 493 Fragments arranged by size: 693 493 44 SmlI C'TyrA_G Cuts at: 0 538 1230 Size: 538 692 SnaBI TAC'GTA Cuts at: 0 966 1230 Size: 966 264 StuI AGG'CCT Cuts at: 0 383 1230 Size: 383 847 StyI C'CwwG_G Cuts at: 0 92 506 653 1230 Size: 92 414 147 577 Fragments arranged by size: 577 414 147 92 TatI w'GTAC_w Cuts at: 0 956 995 1230 Size: 956 39 235 Fragments arranged by size: 956 235 39 XhoI C'TCGA_G Cuts at: 0 538 1230 Size: 538 692 XmaI C'CCGG_G Cuts at: 0 42 735 1230 Size: 42 693 495 Fragments arranged by size: 693 495 42 Enzymes that do cut: AarI AcuI AfeI AflIII AlwNI ApaLI AvaI BamHI BanI BanII BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI DraIII EaeI EarI EciI EcoICRI Eco57MI FalI HaeII Hin4I KasI MluI MmeI MslI MspA1I NarI NcoI NruI PasI PflMI PfoI PmlI PvuII SacI SacII SfcI SfiI SfoI SmaI SmlI SnaBI StuI StyI TatI XhoI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AhdI AleI AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII BaeI BcgI BclI BfrBI BglII BmrI BmtI BpuEI BsaBI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BssHII BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MscI NaeI NdeI NgoMIV NheI NotI NsiI NspI PacI PciI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI SgfI SgrAI SpeI SphI SrfI SspI SwaI TaqII TstI Tth111I XbaI XcmI XmnI ZraI