Restriction map for pFN21AE9533
 (Linear) (Six-base) MAPSORT of: KIEE9533.seq  Check: 1802  from: 1  to: 1230



KIEE9533.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:41  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     704    1230 
   Size:        704     526
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193    1077    1181    1230 
   Size:        193     884     104      49

  Fragments arranged by size: 

                 884     193     104      49
 
AfeI AGC'GCT

Cuts at:      0     397    1230 
   Size:        397     833
 
AflIII A'CryG_T

Cuts at:      0     977    1230 
   Size:        977     253
 
AlwNI CAG_nnn'CTG

Cuts at:      0     420    1230 
   Size:        420     810
 
ApaLI G'TGCA_C

Cuts at:      0     160     340     579    1230 
   Size:        160     180     239     651

  Fragments arranged by size: 

                 651     239     180     160
 
AvaI C'yCGr_G

Cuts at:      0      42     538     735    1230 
   Size:         42     496     197     495

  Fragments arranged by size: 

                 496     495     197      42
 
BamHI G'GATC_C

Cuts at:      0     863    1209    1230 
   Size:        863     346      21

  Fragments arranged by size: 

                 863     346      21
 
BanI G'GyrC_C

Cuts at:      0     197    1230 
   Size:        197    1033
 
BanII G_rGCy'C

Cuts at:      0     272     566    1230 
   Size:        272     294     664

  Fragments arranged by size: 

                 664     294     272
 
BbeI G_GCGC'C

Cuts at:      0     201    1230 
   Size:        201    1029
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     297     814    1104    1230 
   Size:        297     517     290     126

  Fragments arranged by size: 

                 517     297     290     126
 
BbvCI CC'TCA_GC

Cuts at:      0     787    1230 
   Size:        787     443
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1182    1230 
   Size:       1182      48
 
BglI GCCn_nnn'nGGC

Cuts at:      0      27    1230 
   Size:         27    1203
 
BlpI GC'TnA_GC

Cuts at:      0      49     217     370    1050    1230 
   Size:         49     168     153     680     180

  Fragments arranged by size: 

                 680     180     168     153      49
 
Bme1580I G_kGCm'C

Cuts at:      0     164     344     429     583    1230 
   Size:        164     180      85     154     647

  Fragments arranged by size: 

                 647     180     164     154      85
 
BmgBI CAC'GTC

Cuts at:      0     855    1230 
   Size:        855     375
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     585    1230 
   Size:        585     645
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     781     859    1230 
   Size:        781      78     371

  Fragments arranged by size: 

                 781     371      78
 
Bpu10I CC'TnA_GC

Cuts at:      0     385     787    1230 
   Size:        385     402     443

  Fragments arranged by size: 

                 443     402     385
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     787    1230 
   Size:        787     443
 
BsaAI yAC'GTr

Cuts at:      0     165     213     966    1230 
   Size:        165      48     753     264

  Fragments arranged by size: 

                 753     264     165      48
 
BsaHI Gr'CG_yC

Cuts at:      0      59     198     442    1230 
   Size:         59     139     244     788

  Fragments arranged by size: 

                 788     244     139      59
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     815     845    1230 
   Size:        815      30     385

  Fragments arranged by size: 

                 815     385      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     775     790    1052    1077    1230 
   Size:        775      15     262      25     153

  Fragments arranged by size: 

                 775     262     153      25      15
 
BseYI C'CCAG_C

Cuts at:      0      31      66     132     751    1230 
   Size:         31      35      66     619     479

  Fragments arranged by size: 

                 619     479      66      35      31
 
BsiHKAI G_wGCw'C

Cuts at:      0     164     218     272     344     583     650    1139    1230 
   Size:        164      54      54      72     239      67     489      91

  Fragments arranged by size: 

                 489     239     164      91      72      67      54      54
            
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     535    1230 
   Size:        535     695
 
Bsp1286I G_dGCh'C

Cuts at:      0     164     218     272     344     429     566     583     650 
   Size:        164      54      54      72      85     137      17      67

Cuts at:    650    1139    1230 
   Size:        489      91

  Fragments arranged by size: 

                 489     164     137      91      85      72      67      54
                  54      17
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     704    1230 
   Size:        704     526
 
BsrBI CCG'CTC

Cuts at:      0     193    1230 
   Size:        193    1037
 
BsrDI GCAATG_nn'

Cuts at:      0     153    1230 
   Size:        153    1077
 
BsrFI r'CCGG_y

Cuts at:      0     261    1230 
   Size:        261     969
 
BsrGI T'GTAC_A

Cuts at:      0     995    1230 
   Size:        995     235
 
BssSI C'ACGA_G

Cuts at:      0     343     474    1230 
   Size:        343     131     756

  Fragments arranged by size: 

                 756     343     131
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     168     664    1230 
   Size:        168     496     566

  Fragments arranged by size: 

                 566     496     168
 
BstEII G'GTnAC_C

Cuts at:      0    1023    1154    1230 
   Size:       1023     131      76

  Fragments arranged by size: 

                1023     131      76
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     507     759    1185    1230 
   Size:        507     252     426      45

  Fragments arranged by size: 

                 507     426     252      45
 
BstYI r'GATC_y

Cuts at:      0     863    1209    1230 
   Size:        863     346      21

  Fragments arranged by size: 

                 863     346      21
 
BtgI C'CryG_G

Cuts at:      0      16      25     324     362     506    1230 
   Size:         16       9     299      38     144     724

  Fragments arranged by size: 

                 724     299     144      38      16       9
 
DraIII CAC_nnn'GTG

Cuts at:      0     168     213    1230 
   Size:        168      45    1017

  Fragments arranged by size: 

                1017     168      45
 
EaeI y'GGCC_r

Cuts at:      0     509    1230 
   Size:        509     721
 
EarI CTCTTCn'nnn_

Cuts at:      0    1070    1108    1230 
   Size:       1070      38     122

  Fragments arranged by size: 

                1070     122      38
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     562    1230 
   Size:        562     668
 
EcoICRI GAG'CTC

Cuts at:      0     270    1230 
   Size:        270     960
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     193     781     859    1077    1181    1230 
   Size:        193     588      78     218     104      49

  Fragments arranged by size: 

                 588     218     193     104      78      49
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     828    1230 
   Size:        828     402
 
HaeII r_GCGC'y

Cuts at:      0     133     201     229     392     399     472    1230 
   Size:        133      68      28     163       7      73     758

  Fragments arranged by size: 

                 758     163     133      73      68      28       7
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     815     847    1230 
   Size:        815      32     383

  Fragments arranged by size: 

                 815     383      32
 
KasI G'GCGC_C

Cuts at:      0     197    1230 
   Size:        197    1033
 
MluI A'CGCG_T

Cuts at:      0     977    1230 
   Size:        977     253
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62    1230 
   Size:         62    1168
 
MslI CAynn'nnrTG

Cuts at:      0     505    1183    1230 
   Size:        505     678      47

  Fragments arranged by size: 

                 678     505      47
 
MspA1I CmG'CkG

Cuts at:      0      18      27     326    1054    1230 
   Size:         18       9     299     728     176

  Fragments arranged by size: 

                 728     299     176      18       9
 
NarI GG'CG_CC

Cuts at:      0     198    1230 
   Size:        198    1032
 
NcoI C'CATG_G

Cuts at:      0     506    1230 
   Size:        506     724
 
NruI TCG'CGA

Cuts at:      0     573    1230 
   Size:        573     657
 
PasI CC'CwG_GG

Cuts at:      0     299    1230 
   Size:        299     931
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     699    1230 
   Size:        699     531
 
PfoI T'CCnGG_A

Cuts at:      0    1106    1230 
   Size:       1106     124
 
PmlI CAC'GTG

Cuts at:      0     165     213    1230 
   Size:        165      48    1017

  Fragments arranged by size: 

                1017     165      48
 
PvuII CAG'CTG

Cuts at:      0    1054    1230 
   Size:       1054     176
 
SacI G_AGCT'C

Cuts at:      0     272    1230 
   Size:        272     958
 
SacII CC_GC'GG

Cuts at:      0      19      28     327    1230 
   Size:         19       9     299     903

  Fragments arranged by size: 

                 903     299      19       9
 
SfcI C'TryA_G

Cuts at:      0     273    1230 
   Size:        273     957
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0      27    1230 
   Size:         27    1203
 
SfoI GGC'GCC

Cuts at:      0     199    1230 
   Size:        199    1031
 
SmaI CCC'GGG

Cuts at:      0      44     737    1230 
   Size:         44     693     493

  Fragments arranged by size: 

                 693     493      44
 
SmlI C'TyrA_G

Cuts at:      0     538    1230 
   Size:        538     692
 
SnaBI TAC'GTA

Cuts at:      0     966    1230 
   Size:        966     264
 
StuI AGG'CCT

Cuts at:      0     383    1230 
   Size:        383     847
 
StyI C'CwwG_G

Cuts at:      0      92     506     653    1230 
   Size:         92     414     147     577

  Fragments arranged by size: 

                 577     414     147      92
 
TatI w'GTAC_w

Cuts at:      0     956     995    1230 
   Size:        956      39     235

  Fragments arranged by size: 

                 956     235      39
 
XhoI C'TCGA_G

Cuts at:      0     538    1230 
   Size:        538     692
 
XmaI C'CCGG_G

Cuts at:      0      42     735    1230 
   Size:         42     693     495

  Fragments arranged by size: 

                 693     495      42
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII    AlwNI    ApaLI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BglI     BlpI
 Bme1580I    BmgBI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaHI
    BsaXI    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII    BstXI    BstYI
     BtgI   DraIII     EaeI     EarI     EciI  EcoICRI  Eco57MI     FalI
    HaeII    Hin4I     KasI     MluI     MmeI     MslI   MspA1I     NarI
     NcoI     NruI     PasI    PflMI     PfoI     PmlI    PvuII     SacI
    SacII     SfcI     SfiI     SfoI     SmaI     SmlI    SnaBI     StuI
     StyI     TatI     XhoI     XmaI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI     ApoI     AscI     AseI    AsiSI    AvrII
     BaeI     BcgI     BclI    BfrBI    BglII     BmrI     BmtI    BpuEI
    BsaBI    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI
   BssHII    BstBI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
     DraI     DrdI     EagI    EcoNI EcoO109I    EcoRI    EcoRV     FseI
     FspI    FspAI   HincII  HindIII     HpaI     KpnI     MfeI     MscI
     NaeI     NdeI   NgoMIV     NheI     NotI     NsiI     NspI     PacI
     PciI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SgfI    SgrAI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XmnI     ZraI
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