################################## # NO CUT ENZYMES ( 185 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaJI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CfoI Cfr10I ClaI CpoI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI MwoI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AciI 439 CCGC CCGC(-3/-1) AfaI 436 GTAC GT^AC BclI 579 TGATCA T^GATCA BlpI 682 GCTCAGC GC^T[ACGT]AGC Bpu1102I 682 GCTCAGC GC^T[ACGT]AGC BsaI 71 GAGACC GGTCTC(1/5) BsmBI 739 GAGACG CGTCTC(1/5) BsmFI 646 GGGAC GGGAC(10/14) CelII 682 GCTCAGC GC^T[ACGT]AGC EarI 52 GAAGAG CTCTTC(1/4) FbaI 579 TGATCA T^GATCA HaeIII 760 GGCC GG^CC HincII 240 GTTAAC GT[CT]^[GA]AC HindII 240 GTTAAC GT[CT]^[GA]AC HpaI 240 GTTAAC GTT^AAC Ksp632I 52 GAAGAG CTCTTC(1/4) MaeIII 563 GTGAC ^GT[ACGT]AC MspA1I 482 CAGCTG C[AC]G^C[GT]G NlaIV 646 GGGACC GG[ACGT]^[ACGT]CC PflMI 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 445 GAGTC GAGTC(4/5) PstI 25 CTGCAG CTGCA^G PvuII 482 CAGCTG CAG^CTG RsaI 436 GTAC GT^AC SfcI 25 CTGCAG C^T[GA][CT]AG StuI 759 AGGCCT AGG^CCT TaqI 735 TCGA T^CGA TfiI 36 GATTC G^A[AT]TC Tsp45I 563 GTGAC ^GT[GC]AC TthHB8I 735 TCGA T^CGA Van91I 75 CCAAACTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AlwNI 177 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG 443 CAGAGTCTG AvaII 185 GGACC G^G[AT]CC 647 GGACC BbvI 608 GCAGC GCAGC(8/12) 24 GCTGC BseRI 55 GAGGAG GAGGAG(10/8) 202 GAGGAG BsiYI 75 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 587 CCATGCTTAGG BslI 75 CCAAACTTTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 587 CCATGCTTAGG BsmAI 71 GAGAC GTCTC(1/5) 739 GAGAC BsrI 182 ACTGG ACTGG(1/-1) 399 CCAGT BssKI 541 CCAGG ^CC[ACGT]GG 678 CCTGG BstF5I 490 CATCC GGATG(2/0) 453 GGATG BstNI 541 CCAGG CC^[AT]GG 678 CCTGG BstYI 135 GGATCT [GA]^GATC[CT] 300 AGATCC Cfr13I 185 GGACC G^G[ACGT]CC 647 GGACC DraI 94 TTTAAA TTT^AAA 598 TTTAAA EcoRII 541 CCAGG ^CC[AT]GG 678 CCTGG Fnu4HI 24 GCTGC GC^[ACGT]GC 608 GCAGC FokI 490 CATCC GGATG(9/13) 453 GGATG HinfI 36 GATTC G^A[ACGT]TC 445 GAGTC HphI 744 GGTGA GGTGA(8/7) 41 TCACC ItaI 24 GCTGC GC^[ACGT]GC 608 GCAGC MflI 135 GGATCT [GA]^GATC[CT] 300 AGATCC MvaI 541 CCAGG CC^[AT]GG 678 CCTGG Sau96I 185 GGACC G^G[ACGT]CC 647 GGACC ScrFI 541 CCAGG CC^[ACGT]GG 678 CCTGG SfaNI 386 GATGC GCATC(5/9) 501 GATGC TseI 24 GCTGC G^C[AT]GC 608 GCAGC TspRI 181 CACTG CA[GC]TG[ACGT][ACGT]^ 400 CAGTG XhoII 135 GGATCT [GA]^GATC[CT] 300 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AluI AlwI Cac8I DdeI DpnII MboI MboII MnlI MseI NdeII NlaIII Sau3AI Tru9I Tsp509I