################################## # NO CUT ENZYMES ( 164 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FauI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI KspI MaeII MamI MluI MroI MspI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsaI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### Alw44I 152 GTGCAC G^TGCAC AlwI 556 GGATC GGATC(4/5) ApaLI 152 GTGCAC G^TGCAC AspHI 152 GTGCAC G[AT]GC[AT]^C AvaI 740 CCCGAG C^[CT]CG[GA]G BclI 1155 TGATCA T^GATCA BglII 507 AGATCT A^GATCT BpmI 326 CTGGAG CTGGAG(16/14) BsaJI 1082 CCTTGG C^C[ACGT][ACGT]GG BsiEI 62 CGGTCG CG[GA][CT]^CG BsiHKAI 152 GTGCAC G[AT]GC[AT]^C BsoBI 740 CCCGAG C^[CT]CG[GA]G BspHI 1164 TCATGA T^CATGA BsrI 157 CCAGT ACTGG(1/-1) BssKI 853 CCAGG ^CC[ACGT]GG BssSI 1049 CACGAG CACGAG(-5/-1) BstNI 853 CCAGG CC^[AT]GG BstYI 507 AGATCT [GA]^GATC[CT] Cac8I 465 GCTTGC GC[ACGT]^[ACGT]GC Cfr13I 125 GGGCC G^G[ACGT]CC DdeI 478 CTTAG C^T[ACGT]AG DraII 124 AGGGCCT [GA]G^G[ACGT]CC[CT] DraIII 851 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 55 GACATGACGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO109I 124 AGGGCCT [GA]G^G[ACGT]CC[CT] EcoRII 853 CCAGG ^CC[AT]GG EcoT14I 1082 CCTTGG C^C[AT][AT]GG EcoT22I 535 ATGCAT ATGCA^T FbaI 1155 TGATCA T^GATCA MflI 507 AGATCT [GA]^GATC[CT] MslI 1159 CATTCTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvaI 853 CCAGG CC^[AT]GG NdeI 348 CATATG CA^TATG NlaIV 717 GGAACC GG[ACGT]^[ACGT]CC NsiI 535 ATGCAT ATGCA^T PstI 364 CTGCAG CTGCA^G RcaI 1164 TCATGA T^CATGA Sau96I 125 GGGCC G^G[ACGT]CC ScrFI 853 CCAGG CC^[ACGT]GG SexAI 852 ACCAGGT A^CC[AT]GGT SfaNI 382 GATGC GCATC(5/9) SfcI 364 CTGCAG C^T[GA][CT]AG StuI 1079 AGGCCT AGG^CCT StyI 1082 CCTTGG C^C[AT][AT]GG XbaI 510 TCTAGA T^CTAGA XhoII 507 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 698 AAATTT [GA]^AATT[CT] 1023 AAATTT ApoI 698 AAATTT [GA]^AATT[CT] 1023 AAATTT BalI 297 TGGCCA TGG^CCA 389 TGGCCA BmyI 152 GTGCAC G[AGT]GC[ACT]^C 420 GGGCAC BsaI 448 GAGACC GGTCTC(1/5) 817 GGTCTC BseRI 269 CTCCTC GAGGAG(10/8) 972 GAGGAG BsmAI 448 GAGAC GTCTC(1/5) 818 GTCTC Bsp1286I 152 GTGCAC G[AGT]GC[ACT]^C 420 GGGCAC EaeI 297 TGGCCA [CT]^GGCC[GA] 389 TGGCCA EarI 565 GAAGAG CTCTTC(1/4) 969 GAAGAG Ksp632I 565 GAAGAG CTCTTC(1/4) 969 GAAGAG MfeI 88 CAATTG C^AATTG 773 CAATTG MluNI 297 TGGCCA TGG^CCA 389 TGGCCA MscI 297 TGGCCA TGG^CCA 389 TGGCCA MunI 88 CAATTG C^AATTG 773 CAATTG NspI 148 GCATGT [GA]CATG^[CT] 1113 ACATGC PleI 43 GACTC GAGTC(4/5) 1099 GAGTC SmlI 491 CTCAAG C^T[CT][GA]AG 677 CTCAAG Tsp45I 1067 GTGAC ^GT[GC]AC 1101 GTCAC ######################################### # 3 OR MORE CUT ENZYMES ( 29 enzymes) # ######################################### AciI AluI BbvI BfaI BsiYI BslI DpnII Fnu4HI HaeIII HinfI ItaI MaeI MaeIII MboI MboII MnlI MseI MspA1I MwoI NdeII NlaIII Sau3AI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I