(Circular) (Six-base) MAPSORT of: pFN21AE5502.seq Check: 3902 from: 1 to: 5887 pFN21AE5502 5887 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2823 3950 5334 5602 1632 Size: 1191 1127 1384 268 1917 Fragments arranged by size: 1917 1384 1191 1127 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 3630 5202 3630 Size: 1572 4315 ApaLI G'TGCA_C Cuts at: 2157 3944 5100 2157 Size: 1787 1156 2944 Fragments arranged by size: 2944 1787 1156 ApoI r'AATT_y Cuts at: 1493 2703 3028 3196 3422 3574 5683 1493 Size: 1210 325 168 226 152 2109 1697 Fragments arranged by size: 2109 1697 1210 325 226 168 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 2745 3212 1870 Size: 73 802 467 4545 Fragments arranged by size: 4545 802 467 73 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3208 4663 618 Size: 325 217 627 1421 1455 1842 Fragments arranged by size: 1842 1455 1421 627 325 217 BanII G_rGCy'C Cuts at: 1391 3206 1391 Size: 1815 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 3160 1548 Size: 1612 4275 BfrBI ATG'CAT Cuts at: 2542 2542 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2512 5882 2512 Size: 3370 2517 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 2161 2429 3948 5104 1790 Size: 371 268 1519 1156 2573 Fragments arranged by size: 2573 1519 1156 371 268 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3618 4576 442 Size: 416 53 83 399 24 2201 958 1753 Fragments arranged by size: 2201 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2352 4547 1239 Size: 1113 2195 2579 Fragments arranged by size: 2579 2195 1113 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2481 2667 4012 4877 5175 2481 Size: 186 1345 865 298 3193 Fragments arranged by size: 3193 1345 865 298 186 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2447 2828 4550 882 Size: 617 948 381 1722 2219 Fragments arranged by size: 2219 1722 948 617 381 BsaAI yAC'GTr Cuts at: 493 1204 3678 493 Size: 711 2474 2702 Fragments arranged by size: 2702 2474 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2265 2992 1186 Size: 1079 727 4081 Fragments arranged by size: 4081 1079 727 BseYI C'CCAG_C Cuts at: 2075 5090 5586 2075 Size: 3015 496 2376 Fragments arranged by size: 3015 2376 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2070 3337 4098 5126 1540 Size: 275 186 69 1267 761 1028 2301 Fragments arranged by size: 2301 1267 1028 761 275 186 69 BsiHKAI G_wGCw'C Cuts at: 2161 3948 4033 5104 2161 Size: 1787 85 1071 2944 Fragments arranged by size: 2944 1787 1071 85 BsmI GAATG_Cn' Cuts at: 3398 3491 3398 Size: 93 5794 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2161 2429 3206 3948 4033 5104 1391 Size: 399 371 268 777 742 85 1071 2174 Fragments arranged by size: 2174 1071 777 742 399 371 268 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3169 3776 5506 3169 Size: 607 1730 3550 Fragments arranged by size: 3550 1730 607 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 844 Size: 2398 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3054 3947 4959 3054 Size: 893 1012 3982 Fragments arranged by size: 3982 1012 893 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5887 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 5887 BstYI r'GATC_y Cuts at: 1972 2512 3217 3584 3969 3986 5438 5524 5536 Size: 540 705 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 705 540 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2397 3403 4197 4217 2397 Size: 1006 794 20 4067 Fragments arranged by size: 4067 1006 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2386 2421 529 Size: 35 1822 35 3995 Fragments arranged by size: 3995 1822 35 35 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DraIII CAC_nnn'GTG Cuts at: 2861 2861 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2066 4894 809 Size: 507 750 2828 1802 Fragments arranged by size: 2828 1802 750 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2302 2394 3334 Size: 54 1425 50 275 18 472 92 940 Cuts at: 3334 4223 5852 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 940 889 472 275 92 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2565 2969 3817 1353 Size: 1212 404 848 3423 Fragments arranged by size: 3423 1212 848 404 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2352 2823 3950 4547 5334 5602 1239 Size: 393 720 471 1127 597 787 268 1524 Fragments arranged by size: 1524 1127 787 720 597 471 393 268 EcoO109I rG'GnC_Cy Cuts at: 2130 2130 Size: 5887 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 5034 1678 Size: 3356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2649 2681 3035 3067 4528 Size: 32 135 32 1077 32 354 32 1461 Cuts at: 4528 4560 4602 4634 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1461 1077 354 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 2093 2778 3486 2093 Size: 685 708 4494 Fragments arranged by size: 4494 708 685 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 1992 2070 2082 3114 5001 5185 1305 Size: 687 78 12 1032 1887 184 2007 Fragments arranged by size: 2007 1887 1032 687 184 78 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2304 2396 10 Size: 54 1425 343 472 92 3501 Fragments arranged by size: 3501 1425 472 343 92 54 MslI CAynn'nnrTG Cuts at: 518 1166 3168 3845 4204 4363 518 Size: 648 2002 677 359 159 2042 Fragments arranged by size: 2042 2002 677 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2672 2801 3303 3980 5128 5373 1606 Size: 483 583 129 502 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 583 502 483 245 129 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4396 Fragments arranged by size: 4396 939 552 NdeI CA'TA_TG Cuts at: 387 2354 387 Size: 1967 3920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NsiI A_TGCA'T Cuts at: 2544 2544 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2157 3122 3245 4790 1132 Size: 97 928 965 123 1545 2229 Fragments arranged by size: 2229 1545 965 928 123 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 2831 3457 2831 Size: 626 5261 PstI C_TGCA'G Cuts at: 830 2373 3239 830 Size: 1543 866 3478 Fragments arranged by size: 3478 1543 866 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SexAI A'CCwGG_T Cuts at: 2857 2857 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 2369 3235 4370 5051 5242 826 Size: 219 1324 866 1135 681 191 1471 Fragments arranged by size: 1471 1324 1135 866 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2496 2682 3991 4892 5154 820 Size: 197 926 553 186 1309 901 262 1553 Fragments arranged by size: 1553 1309 926 901 553 262 197 186 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 3086 1517 Size: 1569 4318 StyI C'CwwG_G Cuts at: 513 1065 2004 3087 3326 513 Size: 552 939 1083 239 3074 Fragments arranged by size: 3074 1083 939 552 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2089 3914 3931 4084 4269 4714 325 Size: 1255 509 1825 17 153 185 445 1498 Fragments arranged by size: 1825 1498 1255 509 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 5845 96 Size: 274 80 33 51 1443 2156 1712 138 Fragments arranged by size: 2156 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5847 5879 5847 Size: 32 5855 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 2515 3223 2515 Size: 708 5179 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 4016 4016 Size: 5887 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI