################################## # NO CUT ENZYMES ( 195 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BseAI BseRI BsgI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cfr10I Cfr13I ClaI CpoI DpnII DraI DraII DraIII DrdI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NaeI NarI NciI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsaI RsrII SacI SacII SalI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I Tsp509I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AlwNI 55 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 45 GAGCAC G[AT]GC[AT]^C BanII 379 GGGCTC G[GA]GC[CT]^C BbvCI 120 CCTCAGC CCTCAGC(-5/-2) BfaI 164 CTAG C^TAG BlpI 60 GCTGAGC GC^T[ACGT]AGC Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC BsaWI 96 ACCGGA [AT]^CCGG[AT] BsiEI 94 CGACCG CG[GA][CT]^CG BsiHKAI 45 GAGCAC G[AT]GC[AT]^C BssKI 308 CCAGG ^CC[ACGT]GG BstF5I 408 CATCC GGATG(2/0) BstNI 308 CCAGG CC^[AT]GG Cac8I 7 GCCTGC GC[ACGT]^[ACGT]GC CelII 60 GCTGAGC GC^T[ACGT]AGC CfoI 185 GCGC GCG^C DsaI 374 CCATGG C^C[GA][CT]GG EarI 334 CTCTTC CTCTTC(1/4) EcoNI 363 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRII 308 CCAGG ^CC[AT]GG FokI 408 CATCC GGATG(9/13) HaeII 184 AGCGCC [GA]GCGC^[CT] HaeIII 372 GGCC GG^CC HapII 97 CCGG C^CGG HhaI 185 GCGC GCG^C HinP1I 185 GCGC G^CGC HpaII 97 CCGG C^CGG Ksp632I 334 CTCTTC CTCTTC(1/4) MaeI 164 CTAG C^TAG MboII 335 TCTTC GAAGA(8/7) MspA1I 55 CAGCTG C[AC]G^C[GT]G MspI 97 CCGG C^CGG MvaI 308 CCAGG CC^[AT]GG NcoI 374 CCATGG C^CATGG PstI 77 CTGCAG CTGCA^G PvuII 55 CAGCTG CAG^CTG SapI 333 GCTCTTC GCTCTTC(1/4) ScrFI 308 CCAGG CC^[ACGT]GG SfcI 77 CTGCAG C^T[GA][CT]AG TaqI 203 TCGA T^CGA TspRI 80 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 203 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AciI 70 CCGC CCGC(-3/-1) 168 CCGC BmyI 45 GAGCAC G[AGT]GC[ACT]^C 379 GGGCTC BsaJI 35 CCTTGG C^C[ACGT][ACGT]GG 374 CCATGG BsmFI 197 GGGAC GGGAC(10/14) 143 GTCCC Bsp1286I 45 GAGCAC G[AGT]GC[ACT]^C 379 GGGCTC DdeI 61 CTGAG C^T[ACGT]AG 121 CTCAG EcoT14I 35 CCTTGG C^C[AT][AT]GG 374 CCATGG MwoI 119 GCCTCAGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 229 GCTGGGGCTGC NlaIV 287 GGAGCC GG[ACGT]^[ACGT]CC 311 GGAACC StyI 35 CCTTGG C^C[AT][AT]GG 374 CCATGG XcmI 26 CCACTCCCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 239 CCACAGTTGGGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 10 enzymes) # ######################################### AluI BbvI BsiYI BslI BsmAI Fnu4HI ItaI MnlI NlaIII TseI