################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AvaI AviII AvrII BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaJI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstF5I BstPI BstUI BstXI CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT14I EcoT22I FauI FbaI FokI FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MaeII MamI MluI MroI MslI MspA1I MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI SspBI SspI StuI StyI SwaI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 46 enzymes) # ################################### AccI 64 GTATAC GT^[AC][GT]AC Asp700I 774 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 33 GAGCAC G[AT]GC[AT]^C BalI 149 TGGCCA TGG^CCA BamHI 745 GGATCC G^GATCC BbsI 778 GTCTTC GAAGAC(2/6) BbvI 712 GCTGC GCAGC(8/12) BcnI 590 CCCGG CC^[GC]GG BglII 785 AGATCT A^GATCT BpuAI 778 GTCTTC GAAGAC(2/6) BsaI 593 GGTCTC GGTCTC(1/5) BsaWI 29 ACCGGA [AT]^CCGG[AT] BseRI 121 CTCCTC GAGGAG(10/8) BsiHKAI 33 GAGCAC G[AT]GC[AT]^C BspMI 658 ACCTGC ACCTGC(4/8) BsrBI 503 GAGCGG CCGCTC(-3/-3) Bst1107I 64 GTATAC GTA^TAC BstZ17I 64 GTATAC GTA^TAC CfoI 797 GCGC GCG^C DraII 608 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 149 TGGCCA [CT]^GGCC[GA] EarI 445 CTCTTC CTCTTC(1/4) EcoNI 816 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 608 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 250 GAATTC G^AATTC Fnu4HI 712 GCTGC GC^[ACGT]GC HgaI 791 GCGTC GACGC(5/10) HhaI 797 GCGC GCG^C HinP1I 797 GCGC G^CGC HindIII 398 AAGCTT A^AGCTT ItaI 712 GCTGC GC^[ACGT]GC Ksp632I 445 CTCTTC CTCTTC(1/4) MaeIII 328 GTTAC ^GT[ACGT]AC MfeI 188 CAATTG C^AATTG MluNI 149 TGGCCA TGG^CCA MscI 149 TGGCCA TGG^CCA MunI 188 CAATTG C^AATTG NciI 590 CCCGG CC^[GC]GG SapI 444 GCTCTTC GCTCTTC(1/4) SbfI 659 CCTGCAGG CCTGCA^GG SexAI 291 ACCAGGT A^CC[AT]GGT Sse8387I 659 CCTGCAGG CCTGCA^GG TaqI 782 TCGA T^CGA TseI 712 GCTGC G^C[AT]GC TthHB8I 782 TCGA T^CGA XmnI 774 GAAGGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AciI 90 GCGG CCGC(-3/-1) 505 GCGG AcsI 250 GAATTC [GA]^AATT[CT] 412 AAATTT AfaI 18 GTAC GT^AC 306 GTAC AlwNI 635 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG 827 CAGGAGCTG AocI 7 CCTCAGG CC^T[ACGT]AGG 207 CCTGAGG ApoI 250 GAATTC [GA]^AATT[CT] 412 AAATTT AvaII 382 GGTCC G^G[AT]CC 836 GGTCC BmyI 33 GAGCAC G[AGT]GC[ACT]^C 510 GGGCAC BsmFI 285 GTCCC GGGAC(10/14) 582 GTCCC Bsp1286I 33 GAGCAC G[AGT]GC[ACT]^C 510 GGGCAC BspHI 125 TCATGA T^CATGA 176 TCATGA BstNI 43 CCTGG CC^[AT]GG 292 CCAGG BstYI 745 GGATCC [GA]^GATC[CT] 785 AGATCT Bsu36I 7 CCTCAGG CC^T[ACGT]AGG 207 CCTGAGG Cac8I 86 GCTGGC GC[ACGT]^[ACGT]GC 640 GCTGGC Eco81I 7 CCTCAGG CC^T[ACGT]AGG 207 CCTGAGG EcoRII 43 CCTGG ^CC[AT]GG 292 CCAGG HapII 30 CCGG C^CGG 591 CCGG HpaII 30 CCGG C^CGG 591 CCGG HphI 141 GGTGA GGTGA(8/7) 24 TCACC MboII 446 TCTTC GAAGA(8/7) 779 TCTTC MflI 745 GGATCC [GA]^GATC[CT] 785 AGATCT MseI 133 TTAA T^TAA 701 TTAA MspI 30 CCGG C^CGG 591 CCGG MvaI 43 CCTGG CC^[AT]GG 292 CCAGG PleI 244 GACTC GAGTC(4/5) 580 GAGTC PstI 452 CTGCAG CTGCA^G 660 CTGCAG RcaI 125 TCATGA T^CATGA 176 TCATGA RsaI 18 GTAC GT^AC 306 GTAC TfiI 165 GATTC G^A[AT]TC 682 GAATC Tru9I 133 TTAA T^TAA 701 TTAA TspRI 332 CAGTG CA[GC]TG[ACGT][ACGT]^ 515 CAGTG XhoII 745 GGATCC [GA]^GATC[CT] 785 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AluI AlwI BfaI BsiYI BslI BsmAI BsrI BssKI Cfr13I DdeI DpnII HaeIII HinfI MaeI MboI MnlI MwoI NdeII NlaIII NlaIV Sau3AI Sau96I ScrFI SfaNI SfcI Tsp509I