(Circular) (Six-base) MAPSORT of: pFN21AE4321.seq Check: 9828 from: 1 to: 5680 pFN21AE4321 5680 bp With 182 enzymes: SgfI * March 28, 2012 14:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2350 2350 Size: 5680 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2913 3023 1937 Size: 976 110 4594 Fragments arranged by size: 4594 976 110 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2966 3743 5127 5395 1632 Size: 1334 777 1384 268 1917 Fragments arranged by size: 1917 1384 1334 777 268 AfeI AGC'GCT Cuts at: 2193 2193 Size: 5680 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 4579 5556 1203 Size: 3376 977 1327 Fragments arranged by size: 3376 1327 977 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 2675 1166 Size: 1509 4171 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2840 1184 Size: 1656 4024 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaI G_GGCC'C Cuts at: 2048 2048 Size: 5680 ApaLI G'TGCA_C Cuts at: 2815 3737 4893 2815 Size: 922 1156 3602 Fragments arranged by size: 3602 1156 922 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 2065 2079 2105 2166 2559 3005 1870 Size: 73 122 14 26 61 393 446 4545 Fragments arranged by size: 4545 446 393 122 73 61 26 14 BamHI G'GATC_C Cuts at: 3010 3377 3010 Size: 367 5313 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2777 2902 3001 4456 618 Size: 325 217 627 990 125 99 1455 1842 Fragments arranged by size: 1842 1455 990 627 325 217 125 99 BanII G_rGCy'C Cuts at: 1391 2048 2064 2874 2999 1391 Size: 657 16 810 125 4072 Fragments arranged by size: 4072 810 657 125 16 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2629 928 Size: 971 730 3979 Fragments arranged by size: 3979 971 730 BbvCI CC'TCA_GC Cuts at: 2594 2594 Size: 5680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2371 2405 2837 2871 3871 3905 1903 Size: 34 434 34 432 34 1000 34 3678 Fragments arranged by size: 3678 1000 434 432 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 1548 Size: 5680 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2052 4291 Size: 107 122 71 631 605 337 43 2239 Cuts at: 4291 136 Size: 1525 Fragments arranged by size: 2239 1525 631 605 337 122 107 71 43 BglII A'GATC_T Cuts at: 5675 5675 Size: 5680 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2048 2080 2819 2907 3741 4897 1790 Size: 258 32 739 88 834 1156 2573 Fragments arranged by size: 2573 1156 834 739 258 88 32 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3411 4369 442 Size: 416 53 83 399 24 1994 958 1753 Fragments arranged by size: 1994 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2285 4340 1239 Size: 1046 2055 2579 Fragments arranged by size: 2579 2055 1046 Bpu10I CC'TnA_GC Cuts at: 2439 2594 2439 Size: 155 5525 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2858 3805 4670 4968 2858 Size: 947 865 298 3570 Fragments arranged by size: 3570 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2298 2699 4343 882 Size: 617 799 401 1644 2219 Fragments arranged by size: 2219 1644 799 617 401 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2334 4113 4511 4785 4932 5343 2334 Size: 1779 398 274 147 411 2671 Fragments arranged by size: 2671 1779 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2624 2654 1178 Size: 30 165 30 1221 30 4204 Fragments arranged by size: 4204 1221 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2297 1186 Size: 1111 4569 BseYI C'CCAG_C Cuts at: 2194 2279 2782 2829 4883 5379 2194 Size: 85 503 47 2054 496 2495 Fragments arranged by size: 2495 2054 503 496 85 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2113 2255 1163 Size: 573 377 142 4588 Fragments arranged by size: 4588 573 377 142 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2972 3130 3891 4919 1540 Size: 275 186 971 158 761 1028 2301 Fragments arranged by size: 2301 1028 971 761 275 186 158 BsiHKAI G_wGCw'C Cuts at: 2819 2850 3741 3826 4897 2819 Size: 31 891 85 1071 3602 Fragments arranged by size: 3602 1071 891 85 31 BsmI GAATG_Cn' Cuts at: 2239 3191 3284 2239 Size: 952 93 4635 Fragments arranged by size: 4635 952 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 2064 2080 2819 2850 2874 2907 Size: 399 258 16 16 739 31 24 33 Cuts at: 2907 2999 3741 3826 4897 1391 Size: 92 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 739 399 258 92 85 33 31 24 16 16 BspEI T'CCGG_A Cuts at: 2334 5343 2334 Size: 3009 2671 BspHI T'CATG_A Cuts at: 3569 5299 3569 Size: 1730 3950 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2350 3035 844 Size: 1506 685 3489 Fragments arranged by size: 3489 1506 685 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2579 2727 4175 4349 65 Size: 1183 1331 148 1448 174 1396 Fragments arranged by size: 1448 1396 1331 1183 174 148 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5680 BssHII G'CGCG_C Cuts at: 1925 2070 2097 1925 Size: 145 27 5508 Fragments arranged by size: 5508 145 27 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 2216 794 Size: 1422 4258 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2263 2734 2263 Size: 471 5209 BstYI r'GATC_y Cuts at: 1972 3010 3377 3762 3779 5231 5317 5329 5675 Size: 1038 367 385 17 1452 86 12 346 Cuts at: 5675 1972 Size: 1977 Fragments arranged by size: 1977 1452 1038 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2130 2130 Size: 5680 BtgI C'CryG_G Cuts at: 513 1065 2004 2048 2229 2288 2299 2733 513 Size: 552 939 44 181 59 11 434 3460 Fragments arranged by size: 3460 939 552 434 181 59 44 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4941 Fragments arranged by size: 4941 683 56 BtsI GCAGTG_nn' Cuts at: 2142 2681 3196 3990 4010 2142 Size: 539 515 794 20 3812 Fragments arranged by size: 3812 794 539 515 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DraIII CAC_nnn'GTG Cuts at: 2410 2410 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4687 809 Size: 507 3371 1802 Fragments arranged by size: 3371 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2226 2504 2719 Size: 54 1425 50 275 18 396 278 215 Cuts at: 2719 2969 3127 4016 5645 8 Size: 250 158 889 1629 43 Fragments arranged by size: 1629 1425 889 396 278 275 250 215 158 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2969 3127 1537 Size: 275 1157 158 4090 Fragments arranged by size: 4090 1157 275 158 EarI CTCTTCn'nnn_ Cuts at: 1353 2317 2942 3610 1353 Size: 964 625 668 3423 Fragments arranged by size: 3423 964 668 625 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2285 2966 3743 4340 5127 5395 1239 Size: 393 653 681 777 597 787 268 1524 Fragments arranged by size: 1524 787 777 681 653 597 393 268 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2195 4827 1678 Size: 517 2632 2531 Fragments arranged by size: 2632 2531 517 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2878 2910 4321 4353 4395 Size: 32 135 32 1306 32 1411 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 1411 1306 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2933 4794 4978 1305 Size: 1628 1861 184 2007 Fragments arranged by size: 2007 1861 1628 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2721 10 Size: 54 1425 343 889 2969 Fragments arranged by size: 2969 1425 889 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2622 2675 2732 3638 3997 4156 518 Size: 648 1456 53 57 906 359 159 2042 Fragments arranged by size: 2042 1456 906 648 359 159 57 53 MspA1I CmG'CkG Cuts at: 1606 2015 2050 3096 3773 4921 5166 1606 Size: 409 35 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 409 245 35 NcoI C'CATG_G Cuts at: 513 1065 2004 2733 513 Size: 552 939 729 3460 Fragments arranged by size: 3460 939 729 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2203 3038 4583 1132 Size: 97 974 835 1545 2229 Fragments arranged by size: 2229 1545 974 835 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 4579 4579 Size: 5680 PflMI CCAn_nnn'nTGG Cuts at: 1108 2383 2410 1108 Size: 1275 27 4378 Fragments arranged by size: 4378 1275 27 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5680 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PspOMI G'GGCC_C Cuts at: 2044 2044 Size: 5680 PspXI vC'TCGA_Gb Cuts at: 2559 2559 Size: 5680 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 PvuII CAG'CTG Cuts at: 2015 2015 Size: 5680 SacII CC_GC'GG Cuts at: 2051 2051 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SexAI A'CCwGG_T Cuts at: 2406 2406 Size: 5680 SfcI C'TryA_G Cuts at: 826 1045 3028 4163 4844 5035 826 Size: 219 1983 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 2067 2081 2107 3007 1872 Size: 195 14 26 900 4545 Fragments arranged by size: 4545 900 195 26 14 SmlI C'TyrA_G Cuts at: 820 1017 1943 2559 2873 3784 4685 4947 820 Size: 197 926 616 314 911 901 262 1553 Fragments arranged by size: 1553 926 911 901 616 314 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 2203 3038 1132 Size: 1071 835 3774 Fragments arranged by size: 3774 1071 835 SrfI GCCC'GGGC Cuts at: 2107 2107 Size: 5680 SspI AAT'ATT Cuts at: 5 52 3604 5 Size: 47 3552 2081 Fragments arranged by size: 3552 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 2004 2316 2733 2805 2976 3119 513 Size: 552 939 312 417 72 171 143 3074 Fragments arranged by size: 3074 939 552 417 312 171 143 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2683 3707 3724 3877 4062 4507 325 Size: 1255 1103 1024 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1103 1024 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3926 5638 96 Size: 274 80 33 51 1443 1949 1712 138 Fragments arranged by size: 1949 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5640 5672 5640 Size: 32 5648 Tth111I GACn'n_nGTC Cuts at: 1316 2633 1316 Size: 1317 4363 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 2559 1943 Size: 616 5064 XmaI C'CCGG_G Cuts at: 1870 2065 2079 2105 3005 1870 Size: 195 14 26 900 4545 Fragments arranged by size: 4545 900 195 26 14 XmnI GAAnn'nnTTC Cuts at: 2516 3809 2516 Size: 1293 4387 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PsrI RsrII SacI SanDI SapI SfiI SfoI SwaI XcmI