(Linear) (Six-base) MAPSORT of: KIEE4321.seq Check: 822 from: 1 to: 975 KIEE4321.orf With 182 enzymes: SgfI * March 22, 2012 17:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 345 975 Size: 345 630 AccI GT'mk_AC Cuts at: 0 908 975 Size: 908 67 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 961 975 Size: 961 14 AfeI AGC'GCT Cuts at: 0 188 975 Size: 188 787 AleI CACnn'nnGTG Cuts at: 0 670 975 Size: 670 305 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 835 975 Size: 835 140 ApaI G_GGCC'C Cuts at: 0 43 975 Size: 43 932 ApaLI G'TGCA_C Cuts at: 0 810 975 Size: 810 165 AvaI C'yCGr_G Cuts at: 0 60 74 100 161 554 975 Size: 60 14 26 61 393 421 Fragments arranged by size: 421 393 61 60 26 14 BanI G'GyrC_C Cuts at: 0 772 897 975 Size: 772 125 78 Fragments arranged by size: 772 125 78 BanII G_rGCy'C Cuts at: 0 43 59 869 975 Size: 43 16 810 106 Fragments arranged by size: 810 106 43 16 BbsI GAAGACnn'nnnn_ Cuts at: 0 624 975 Size: 624 351 BbvCI CC'TCA_GC Cuts at: 0 589 975 Size: 589 386 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 366 400 832 866 975 Size: 366 34 432 34 109 Fragments arranged by size: 432 366 109 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 47 975 Size: 47 928 Bme1580I G_kGCm'C Cuts at: 0 43 75 814 902 975 Size: 43 32 739 88 73 Fragments arranged by size: 739 88 73 43 32 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 975 Size: 280 695 Bpu10I CC'TnA_GC Cuts at: 0 434 589 975 Size: 434 155 386 Fragments arranged by size: 434 386 155 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 853 975 Size: 853 122 BsaI GGTCTCn'nnnn_ Cuts at: 0 293 694 975 Size: 293 401 281 Fragments arranged by size: 401 293 281 BsaWI w'CCGG_w Cuts at: 0 329 975 Size: 329 646 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 619 649 975 Size: 619 30 326 Fragments arranged by size: 619 326 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 292 975 Size: 292 683 BseYI C'CCAG_C Cuts at: 0 189 274 777 824 975 Size: 189 85 503 47 151 Fragments arranged by size: 503 189 151 85 47 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 108 250 975 Size: 108 142 725 Fragments arranged by size: 725 142 108 BsiEI CG_ry'CG Cuts at: 0 967 975 Size: 967 8 BsiHKAI G_wGCw'C Cuts at: 0 814 845 975 Size: 814 31 130 Fragments arranged by size: 814 130 31 BsmI GAATG_Cn' Cuts at: 0 234 975 Size: 234 741 Bsp1286I G_dGCh'C Cuts at: 0 43 59 75 814 845 869 902 975 Size: 43 16 16 739 31 24 33 73 Fragments arranged by size: 739 73 43 33 31 24 16 16 BspEI T'CCGG_A Cuts at: 0 329 975 Size: 329 646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 345 975 Size: 345 630 BsrDI GCAATG_nn' Cuts at: 0 574 722 975 Size: 574 148 253 Fragments arranged by size: 574 253 148 BssHII G'CGCG_C Cuts at: 0 65 92 975 Size: 65 27 883 Fragments arranged by size: 883 65 27 BstAPI GCAn_nnn'nTGC Cuts at: 0 211 975 Size: 211 764 BstXI CCAn_nnnn'nTGG Cuts at: 0 258 729 975 Size: 258 471 246 Fragments arranged by size: 471 258 246 Bsu36I CC'TnA_GG Cuts at: 0 125 975 Size: 125 850 BtgI C'CryG_G Cuts at: 0 43 224 283 294 728 975 Size: 43 181 59 11 434 247 Fragments arranged by size: 434 247 181 59 43 11 BtsI GCAGTG_nn' Cuts at: 0 137 676 975 Size: 137 539 299 Fragments arranged by size: 539 299 137 DraIII CAC_nnn'GTG Cuts at: 0 405 975 Size: 405 570 EaeI y'GGCC_r Cuts at: 0 221 499 714 964 975 Size: 221 278 215 250 11 Fragments arranged by size: 278 250 221 215 11 EagI C'GGCC_G Cuts at: 0 964 975 Size: 964 11 EarI CTCTTCn'nnn_ Cuts at: 0 312 937 975 Size: 312 625 38 Fragments arranged by size: 625 312 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 280 961 975 Size: 280 681 14 Fragments arranged by size: 681 280 14 HaeII r_GCGC'y Cuts at: 0 190 975 Size: 190 785 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 873 905 975 Size: 873 32 70 Fragments arranged by size: 873 70 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 928 975 Size: 928 47 MscI TGG'CCA Cuts at: 0 716 975 Size: 716 259 MslI CAynn'nnrTG Cuts at: 0 617 670 727 975 Size: 617 53 57 248 Fragments arranged by size: 617 248 57 53 MspA1I CmG'CkG Cuts at: 0 10 45 975 Size: 10 35 930 Fragments arranged by size: 930 35 10 NcoI C'CATG_G Cuts at: 0 728 975 Size: 728 247 NspI r_CATG'y Cuts at: 0 198 975 Size: 198 777 PflMI CCAn_nnn'nTGG Cuts at: 0 378 405 975 Size: 378 27 570 Fragments arranged by size: 570 378 27 PspOMI G'GGCC_C Cuts at: 0 39 975 Size: 39 936 PspXI vC'TCGA_Gb Cuts at: 0 554 975 Size: 554 421 PvuII CAG'CTG Cuts at: 0 10 975 Size: 10 965 SacII CC_GC'GG Cuts at: 0 46 975 Size: 46 929 SexAI A'CCwGG_T Cuts at: 0 401 975 Size: 401 574 SmaI CCC'GGG Cuts at: 0 62 76 102 975 Size: 62 14 26 873 Fragments arranged by size: 873 62 26 14 SmlI C'TyrA_G Cuts at: 0 554 868 975 Size: 554 314 107 Fragments arranged by size: 554 314 107 SphI G_CATG'C Cuts at: 0 198 975 Size: 198 777 SrfI GCCC'GGGC Cuts at: 0 102 975 Size: 102 873 StyI C'CwwG_G Cuts at: 0 311 728 800 975 Size: 311 417 72 175 Fragments arranged by size: 417 311 175 72 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 678 975 Size: 678 297 Tth111I GACn'n_nGTC Cuts at: 0 628 975 Size: 628 347 XhoI C'TCGA_G Cuts at: 0 554 975 Size: 554 421 XmaI C'CCGG_G Cuts at: 0 60 74 100 975 Size: 60 14 26 875 Fragments arranged by size: 875 60 26 14 XmnI GAAnn'nnTTC Cuts at: 0 511 975 Size: 511 464 Enzymes that do cut: AarI AccI AcuI AfeI AleI AlfI ApaI ApaLI AvaI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BssHII BstAPI BstXI Bsu36I BtgI BtsI DraIII EaeI EagI EarI Eco57MI HaeII Hin4I MmeI MscI MslI MspA1I NcoI NspI PflMI PspOMI PspXI PvuII SacII SexAI SmaI SmlI SphI SrfI StyI TaqII Tth111I XhoI XmaI XmnI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI BsiWI BsmBI BspHI BsrBI BsrFI BsrGI BssSI BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI RsrII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SspI StuI SwaI TatI TstI XbaI XcmI ZraI