################################## # NO CUT ENZYMES ( 118 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BbeI BbvCI BciVI BclI BfrI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspLU11I BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HpaI KasI KpnI MamI MfeI MluI MroI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PstI PvuI RsrII SalI SbfI SfcI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AcsI 327 GAATTC [GA]^AATT[CT] AlwNI 394 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 327 GAATTC [GA]^AATT[CT] AvaI 332 CCCGGG C^[CT]CG[GA]G BalI 192 TGGCCA TGG^CCA BbrPI 1216 CACGTG CAC^GTG BbsI 1110 GAAGAC GAAGAC(2/6) BcgI 435 GCATACTACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 642 GCTCAGC GC^T[ACGT]AGC Bpu1102I 642 GCTCAGC GC^T[ACGT]AGC BpuAI 1110 GAAGAC GAAGAC(2/6) BsaAI 1216 CACGTG [CT]AC^GT[GA] BsaI 478 GAGACC GGTCTC(1/5) BseRI 1303 CTCCTC GAGGAG(10/8) BsgI 1007 GTGCAG GTGCAG(16/14) BsmI 366 GAATGC GAATGC(1/-1) BsoBI 332 CCCGGG C^[CT]CG[GA]G Bsp1407I 1101 TGTACA T^GTACA BspHI 701 TCATGA T^CATGA BsrBI 82 GAGCGG CCGCTC(-3/-3) BsrDI 829 CATTGC GCAATG(2/0) BsrGI 1101 TGTACA T^GTACA BstAPI 824 GCACCCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 985 CCAGGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 642 GCTCAGC GC^T[ACGT]AGC CfoI 18 GCGC GCG^C EaeI 192 TGGCCA [CT]^GGCC[GA] EcoRI 327 GAATTC G^AATTC EcoT14I 568 CCATGG C^C[AT][AT]GG HapII 333 CCGG C^CGG HhaI 18 GCGC GCG^C HinP1I 18 GCGC G^CGC HindIII 1162 AAGCTT A^AGCTT HpaII 333 CCGG C^CGG KspI 263 CCGCGG CCGC^GG MaeIII 247 GTGAC ^GT[ACGT]AC MluNI 192 TGGCCA TGG^CCA MscI 192 TGGCCA TGG^CCA MspI 333 CCGG C^CGG NcoI 568 CCATGG C^CATGG NruI 74 TCGCGA TCG^CGA PmaCI 1216 CACGTG CAC^GTG PmlI 1216 CACGTG CAC^GTG PvuII 12 CAGCTG CAG^CTG RcaI 701 TCATGA T^CATGA SacII 263 CCGCGG CCGC^GG SapI 573 GAAGAGC GCTCTTC(1/4) ScaI 674 AGTACT AGT^ACT SexAI 1134 ACCAGGT A^CC[AT]GGT SfaNI 537 GCATC GCATC(5/9) SmaI 332 CCCGGG CCC^GGG SspBI 1101 TGTACA T^GTACA StuI 803 AGGCCT AGG^CCT StyI 568 CCATGG C^C[AT][AT]GG Tsp45I 247 GTGAC ^GT[GC]AC Tsp509I 328 AATT ^AATT XmaCI 332 CCCGGG C^CCGGG XmaI 332 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### ApaI 63 GGGCCC GGGCC^C 988 GGGCCC AspHI 547 GAGCTC G[AT]GC[AT]^C 745 GAGCTC BcnI 332 CCCGG CC^[GC]GG 333 CCGGG BglII 911 AGATCT A^GATCT 1037 AGATCT BpmI 544 CTGGAG CTGGAG(16/14) 742 CTGGAG BsiHKAI 547 GAGCTC G[AT]GC[AT]^C 745 GAGCTC BsmAI 478 GAGAC GTCTC(1/5) 616 GTCTC BsmFI 161 GTCCC GGGAC(10/14) 259 GTCCC BspMI 1076 ACCTGC ACCTGC(4/8) 57 GCAGGT BsrI 399 ACTGG ACTGG(1/-1) 651 CCAGT DsaI 263 CCGCGG C^C[GA][CT]GG 568 CCATGG EcoNI 930 CCTAAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1077 CCTGCCCCAGG HgaI 137 GCGTC GACGC(5/10) 257 GCGTC HincII 378 GTCAAC GT[CT]^[GA]AC 1279 GTTGAC HindII 378 GTCAAC GT[CT]^[GA]AC 1279 GTTGAC MaeII 944 ACGT A^CGT 1217 ACGT MseI 46 TTAA T^TAA 848 TTAA MspA1I 12 CAGCTG C[AC]G^C[GT]G 263 CCGCGG NciI 332 CCCGG CC^[GC]GG 333 CCGGG PflMI 393 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 450 CCAAGCTCTGG PleI 249 GACTC GAGTC(4/5) 50 GAGTC PpuMI 335 GGGTCCT [GA]G^G[AT]CC[CT] 851 AGGACCC SacI 547 GAGCTC GAGCT^C 745 GAGCTC SmlI 790 CTCAAG C^T[CT][GA]AG 1306 CTCAAG Tru9I 46 TTAA T^TAA 848 TTAA Van91I 393 CCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 450 CCAAGCTCTGG XcmI 389 CCAACCAGCTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 810 CCACTCACACCCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AvaII BanI BanII BbvI BfaI BmyI BsaJI BsiYI BslI Bsp1286I BssKI BstF5I BstNI BstUI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MboI MboII MflI MnlI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TfiI TseI TspRI XhoII