(Circular) (Six-base) MAPSORT of: pFN21AE5508.seq Check: 6292 from: 1 to: 6016 pFN21AE5508 6016 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 3090 2053 Size: 1037 4979 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3826 278 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3359 1937 Size: 1422 4594 Acc65I G'GTAC_C Cuts at: 3337 3337 Size: 6016 AclI AA'CG_TT Cuts at: 1563 4143 4516 1563 Size: 2580 373 3063 Fragments arranged by size: 3063 2580 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2790 4079 5463 5731 1632 Size: 1158 1289 1384 268 1917 Fragments arranged by size: 1917 1384 1289 1158 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5819 AflIII A'CryG_T Cuts at: 1203 4915 5892 1203 Size: 3712 977 1327 Fragments arranged by size: 3712 1327 977 AgeI A'CCGG_T Cuts at: 4847 4847 Size: 6016 AhdI GACnn_n'nnGTC Cuts at: 4745 4745 Size: 6016 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6016 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6016 AlwNI CAG_nnn'CTG Cuts at: 2404 3759 5331 2404 Size: 1355 1572 3089 Fragments arranged by size: 3089 1572 1355 ApaI G_GGCC'C Cuts at: 2072 2997 2072 Size: 925 5091 ApaLI G'TGCA_C Cuts at: 4073 5229 4073 Size: 1156 4860 ApoI r'AATT_y Cuts at: 1493 2332 3325 3551 3703 5812 1493 Size: 839 993 226 152 2109 1697 Fragments arranged by size: 2109 1697 993 839 226 152 AseI AT'TA_AT Cuts at: 160 1032 4570 160 Size: 872 3538 1606 Fragments arranged by size: 3538 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 AvaI C'yCGr_G Cuts at: 1870 1943 2337 3341 1870 Size: 73 394 1004 4545 Fragments arranged by size: 4545 1004 394 73 BamHI G'GATC_C Cuts at: 3346 3713 3346 Size: 367 5649 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 3094 3175 3337 4792 Size: 325 217 627 1041 266 81 162 1455 Cuts at: 4792 618 Size: 1842 Fragments arranged by size: 1842 1455 1041 627 325 266 217 162 81 BanII G_rGCy'C Cuts at: 1391 2072 2207 2368 2556 2754 2997 3335 1391 Size: 681 135 161 188 198 243 338 4072 Fragments arranged by size: 4072 681 338 243 198 188 161 135 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3122 928 Size: 971 1223 3822 Fragments arranged by size: 3822 1223 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2429 2463 4207 4241 1903 Size: 34 492 34 1744 34 3678 Fragments arranged by size: 3678 1744 492 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3908 5118 3908 Size: 1210 4806 BclI T'GATC_A Cuts at: 1548 1548 Size: 6016 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4627 136 Size: 107 122 71 631 605 2955 1525 Fragments arranged by size: 2955 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2916 3042 6011 2916 Size: 126 2969 2921 Fragments arranged by size: 2969 2921 126 BlpI GC'TnA_GC Cuts at: 2648 3433 2648 Size: 785 5231 Bme1580I G_kGCm'C Cuts at: 1790 2072 2997 3180 4077 5233 1790 Size: 282 925 183 897 1156 2573 Fragments arranged by size: 2573 1156 925 897 282 183 BmgBI CAC'GTC Cuts at: 1318 2950 1318 Size: 1632 4384 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2414 2650 3747 Size: 416 53 83 399 24 997 236 1097 Cuts at: 3747 4705 442 Size: 958 1753 Fragments arranged by size: 1753 1097 997 958 416 399 236 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6016 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2372 3086 2372 Size: 714 5302 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2570 2768 4676 1239 Size: 1331 198 1908 2579 Fragments arranged by size: 2579 1908 1331 198 Bpu10I CC'TnA_GC Cuts at: 2663 2936 2663 Size: 273 5743 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2780 3296 4141 5006 5304 2780 Size: 516 845 865 298 3492 Fragments arranged by size: 3492 865 845 516 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2477 4679 882 Size: 617 978 2202 2219 Fragments arranged by size: 2219 2202 978 617 BsaAI yAC'GTr Cuts at: 493 1204 3223 3807 493 Size: 711 2019 584 2702 Fragments arranged by size: 2702 2019 711 584 BsaBI GATnn'nnATC Cuts at: 6010 6010 Size: 6016 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3823 4205 4840 275 Size: 53 83 186 936 2290 382 635 1451 Fragments arranged by size: 2290 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4449 4847 5121 5268 5679 4449 Size: 398 274 147 411 4786 Fragments arranged by size: 4786 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3212 3242 1178 Size: 30 165 30 1809 30 3952 Fragments arranged by size: 3952 1809 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3299 1186 Size: 2113 3903 BseYI C'CCAG_C Cuts at: 2015 2840 2845 2985 5219 5715 2015 Size: 825 5 140 2234 496 2316 Fragments arranged by size: 2316 2234 825 496 140 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3033 1163 Size: 573 1297 4146 Fragments arranged by size: 4146 1297 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3466 4227 5255 1540 Size: 275 186 1465 761 1028 2301 Fragments arranged by size: 2301 1465 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2556 2754 4077 4162 5233 2556 Size: 198 1323 85 1071 3339 Fragments arranged by size: 3339 1323 1071 198 85 BsmI GAATG_Cn' Cuts at: 2377 3527 3620 2377 Size: 1150 93 4773 Fragments arranged by size: 4773 1150 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2207 2368 2556 2754 2997 3180 Size: 399 282 135 161 188 198 243 183 Cuts at: 3180 3335 4077 4162 5233 1391 Size: 155 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 282 243 198 188 183 161 155 135 85 BspEI T'CCGG_A Cuts at: 5679 5679 Size: 6016 BspHI T'CATG_A Cuts at: 2706 3905 5635 2706 Size: 1199 1730 3087 Fragments arranged by size: 3087 1730 1199 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3090 3371 844 Size: 1209 1037 281 3489 Fragments arranged by size: 3489 1209 1037 281 BsrBI CCG'CTC Cuts at: 1394 1823 2089 3903 1394 Size: 429 266 1814 3507 Fragments arranged by size: 3507 1814 429 266 BsrDI GCAATG_nn' Cuts at: 65 1248 2833 4511 4685 65 Size: 1183 1585 1678 174 1396 Fragments arranged by size: 1678 1585 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4660 4847 1700 Size: 110 2850 187 2869 Fragments arranged by size: 2869 2850 187 110 BsrGI T'GTAC_A Cuts at: 96 3106 96 Size: 3010 3006 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6016 BssSI C'ACGA_G Cuts at: 4076 5088 4076 Size: 1012 5004 BstAPI GCAn_nnn'nTGC Cuts at: 794 2835 794 Size: 2041 3975 BstBI TT'CG_AA Cuts at: 4820 4820 Size: 6016 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6016 BstXI CCAn_nnnn'nTGG Cuts at: 2997 2997 Size: 6016 BstYI r'GATC_y Cuts at: 1972 2569 2688 2916 3042 3346 3713 4098 4115 Size: 597 119 228 126 304 367 385 17 Cuts at: 4115 5567 5653 5665 6011 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 597 385 367 346 304 228 126 119 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2268 2573 513 Size: 552 939 264 305 3956 Fragments arranged by size: 3956 939 552 305 264 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3294 487 Size: 683 56 2068 3209 Fragments arranged by size: 3209 2068 683 56 BtsI GCAGTG_nn' Cuts at: 3129 3532 4326 4346 3129 Size: 403 794 20 4799 Fragments arranged by size: 4799 794 403 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6016 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5981 DraI TTT'AAA Cuts at: 3320 3320 Size: 6016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5023 809 Size: 507 3707 1802 Fragments arranged by size: 3707 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2197 3463 4352 Size: 54 1425 50 275 18 367 1266 889 Cuts at: 4352 5981 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1266 889 367 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3463 1537 Size: 275 1651 4090 Fragments arranged by size: 4090 1651 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2531 2573 3033 3946 1353 Size: 1178 42 460 913 3423 Fragments arranged by size: 3423 1178 913 460 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3281 4603 4977 5123 387 Size: 1064 138 1692 1322 374 146 1280 Fragments arranged by size: 1692 1322 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2554 2752 2554 Size: 198 5818 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2570 2768 2790 4079 4676 5463 5731 Size: 393 938 198 22 1289 597 787 268 Cuts at: 5731 1239 Size: 1524 Fragments arranged by size: 1524 1289 938 787 597 393 268 198 22 EcoNI CCTnn'n_nnAGG Cuts at: 2939 3086 2939 Size: 147 5869 EcoO109I rG'GnC_Cy Cuts at: 2069 2341 2857 2993 2994 2069 Size: 272 516 136 1 5091 Fragments arranged by size: 5091 516 272 136 1 EcoRI G'AATT_C Cuts at: 2332 3325 3703 2332 Size: 993 378 4645 Fragments arranged by size: 4645 993 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2935 2935 Size: 6016 FspI TGC'GCA Cuts at: 4522 4522 Size: 6016 HaeII r_GCGC'y Cuts at: 1678 5163 1678 Size: 3485 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4657 4689 4731 4763 1373 Size: 32 135 32 3085 32 42 32 2626 Fragments arranged by size: 3085 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2385 3286 3360 3606 669 Size: 988 281 447 901 74 246 3079 Fragments arranged by size: 3079 988 901 447 281 246 74 HindIII A'AGCT_T Cuts at: 748 3167 748 Size: 2419 3597 HpaI GTT'AAC Cuts at: 3606 3606 Size: 6016 KpnI G_GTAC'C Cuts at: 3341 3341 Size: 6016 MfeI C'AATT_G Cuts at: 3615 3615 Size: 6016 MluI A'CGCG_T Cuts at: 5892 5892 Size: 6016 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2146 2418 2544 3184 5130 5314 1305 Size: 841 272 126 640 1946 184 2007 Fragments arranged by size: 2007 1946 841 640 272 184 126 MscI TGG'CCA Cuts at: 10 64 1489 1832 2199 10 Size: 54 1425 343 367 3827 Fragments arranged by size: 3827 1425 367 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3974 4333 4492 518 Size: 648 2808 359 159 2042 Fragments arranged by size: 2808 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2019 2270 3432 4109 5257 5502 1606 Size: 413 251 1162 677 1148 245 2120 Fragments arranged by size: 2120 1162 1148 677 413 251 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2573 513 Size: 552 939 569 3956 Fragments arranged by size: 3956 939 569 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6016 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6016 NotI GC'GGCC_GC Cuts at: 3463 3463 Size: 6016 NruI TCG'CGA Cuts at: 2081 2081 Size: 6016 NspI r_CATG'y Cuts at: 1132 1229 3374 4919 1132 Size: 97 2145 1545 2229 Fragments arranged by size: 2229 2145 1545 97 PasI CC'CwG_GG Cuts at: 1348 2997 1348 Size: 1649 4367 PciI A'CATG_T Cuts at: 4915 4915 Size: 6016 PflMI CCAn_nnn'nTGG Cuts at: 1108 2404 2461 1108 Size: 1296 57 4663 Fragments arranged by size: 4663 1296 57 PfoI T'CCnGG_A Cuts at: 2429 2745 2429 Size: 316 5700 PmeI GTTT'AAAC Cuts at: 3320 3320 Size: 6016 PmlI CAC'GTG Cuts at: 3223 3223 Size: 6016 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4085 4117 5598 5630 4085 Size: 32 1481 32 4471 Fragments arranged by size: 4471 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2341 2857 2341 Size: 516 5500 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6016 PsiI TTA'TAA Cuts at: 3586 3586 Size: 6016 PspOMI G'GGCC_C Cuts at: 2068 2993 2068 Size: 925 5091 PstI C_TGCA'G Cuts at: 830 3368 830 Size: 2538 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6016 PvuII CAG'CTG Cuts at: 2019 2019 Size: 6016 SacI G_AGCT'C Cuts at: 2556 2754 2556 Size: 198 5818 SacII CC_GC'GG Cuts at: 2271 2271 Size: 6016 SalI G'TCGA_C Cuts at: 1936 3358 1936 Size: 1422 4594 SapI GCTCTTCn'nnn_ Cuts at: 2573 2573 Size: 6016 SbfI CC_TGCA'GG Cuts at: 3368 3368 Size: 6016 ScaI AGT'ACT Cuts at: 2681 4264 5976 2681 Size: 1583 1712 2721 Fragments arranged by size: 2721 1712 1583 SexAI A'CCwGG_T Cuts at: 3139 3139 Size: 6016 SfcI C'TryA_G Cuts at: 826 1045 3364 4499 5180 5371 826 Size: 219 2319 1135 681 191 1471 Fragments arranged by size: 2319 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6016 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6016 SmaI CCC'GGG Cuts at: 1872 2339 3343 1872 Size: 467 1004 4545 Fragments arranged by size: 4545 1004 467 SmlI C'TyrA_G Cuts at: 820 1017 1943 2795 3311 4120 5021 5283 820 Size: 197 926 852 516 809 901 262 1553 Fragments arranged by size: 1553 926 901 852 809 516 262 197 SnaBI TAC'GTA Cuts at: 493 3807 493 Size: 3314 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6016 SphI G_CATG'C Cuts at: 1132 3374 1132 Size: 2242 3774 SspI AAT'ATT Cuts at: 5 52 3940 5 Size: 47 3888 2081 Fragments arranged by size: 3888 2081 47 StuI AGG'CCT Cuts at: 1517 2810 1517 Size: 1293 4723 StyI C'CwwG_G Cuts at: 513 1065 2004 2573 3455 513 Size: 552 939 569 882 3074 Fragments arranged by size: 3074 939 882 569 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2846 4043 4060 4213 4398 4843 325 Size: 1255 1266 1197 17 153 185 445 1498 Fragments arranged by size: 1498 1266 1255 1197 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2679 3106 4262 Size: 274 80 33 51 1443 702 427 1156 Cuts at: 4262 5974 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1156 702 427 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3212 3244 5976 6008 3212 Size: 32 2732 32 3220 Fragments arranged by size: 3220 2732 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6016 XbaI T'CTAG_A Cuts at: 3352 3352 Size: 6016 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2401 2822 2401 Size: 421 5595 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6016 XmaI C'CCGG_G Cuts at: 1870 2337 3341 1870 Size: 467 1004 4545 Fragments arranged by size: 4545 1004 467 XmnI GAAnn'nnTTC Cuts at: 4145 4145 Size: 6016 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3824 276 Size: 53 83 186 936 2290 2468 Fragments arranged by size: 2468 2290 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI