(Linear) (Six-base) MAPSORT of: KIEE5508.seq Check: 4154 from: 1 to: 1311 KIEE5508.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 48 1085 1311 Size: 48 1037 226 Fragments arranged by size: 1037 226 48 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 785 1311 Size: 785 526 AlwNI CAG_nnn'CTG Cuts at: 0 399 1311 Size: 399 912 ApaI G_GGCC'C Cuts at: 0 67 992 1311 Size: 67 925 319 Fragments arranged by size: 925 319 67 ApoI r'AATT_y Cuts at: 0 327 1311 Size: 327 984 AvaI C'yCGr_G Cuts at: 0 332 1311 Size: 332 979 BanI G'GyrC_C Cuts at: 0 823 1089 1170 1311 Size: 823 266 81 141 Fragments arranged by size: 823 266 141 81 BanII G_rGCy'C Cuts at: 0 67 202 363 551 749 992 1311 Size: 67 135 161 188 198 243 319 Fragments arranged by size: 319 243 198 188 161 135 67 BbsI GAAGACnn'nnnn_ Cuts at: 0 1117 1311 Size: 1117 194 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 424 458 1311 Size: 424 34 853 Fragments arranged by size: 853 424 34 BglII A'GATC_T Cuts at: 0 911 1037 1311 Size: 911 126 274 Fragments arranged by size: 911 274 126 BlpI GC'TnA_GC Cuts at: 0 643 1311 Size: 643 668 Bme1580I G_kGCm'C Cuts at: 0 67 992 1175 1311 Size: 67 925 183 136 Fragments arranged by size: 925 183 136 67 BmgBI CAC'GTC Cuts at: 0 945 1311 Size: 945 366 BmrI ACTGGGnnnn_n' Cuts at: 0 409 645 1311 Size: 409 236 666 Fragments arranged by size: 666 409 236 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 367 1081 1311 Size: 367 714 230 Fragments arranged by size: 714 367 230 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 763 1311 Size: 565 198 548 Fragments arranged by size: 565 548 198 Bpu10I CC'TnA_GC Cuts at: 0 658 931 1311 Size: 658 273 380 Fragments arranged by size: 658 380 273 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 775 1291 1311 Size: 775 516 20 Fragments arranged by size: 775 516 20 BsaI GGTCTCn'nnnn_ Cuts at: 0 472 1311 Size: 472 839 BsaAI yAC'GTr Cuts at: 0 1218 1311 Size: 1218 93 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1207 1237 1311 Size: 1207 30 74 Fragments arranged by size: 1207 74 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1294 1311 Size: 1294 17 BseYI C'CCAG_C Cuts at: 0 10 835 840 980 1311 Size: 10 825 5 140 331 Fragments arranged by size: 825 331 140 10 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1028 1311 Size: 1028 283 BsiHKAI G_wGCw'C Cuts at: 0 551 749 1311 Size: 551 198 562 Fragments arranged by size: 562 551 198 BsmI GAATG_Cn' Cuts at: 0 372 1311 Size: 372 939 Bsp1286I G_dGCh'C Cuts at: 0 67 202 363 551 749 992 1175 1311 Size: 67 135 161 188 198 243 183 136 Fragments arranged by size: 243 198 188 183 161 136 135 67 BspHI T'CATG_A Cuts at: 0 701 1311 Size: 701 610 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 48 1085 1311 Size: 48 1037 226 Fragments arranged by size: 1037 226 48 BsrBI CCG'CTC Cuts at: 0 84 1311 Size: 84 1227 BsrDI GCAATG_nn' Cuts at: 0 828 1311 Size: 828 483 BsrGI T'GTAC_A Cuts at: 0 1101 1311 Size: 1101 210 BstAPI GCAn_nnn'nTGC Cuts at: 0 830 1311 Size: 830 481 BstXI CCAn_nnnn'nTGG Cuts at: 0 992 1311 Size: 992 319 BstYI r'GATC_y Cuts at: 0 564 683 911 1037 1311 Size: 564 119 228 126 274 Fragments arranged by size: 564 274 228 126 119 BtgI C'CryG_G Cuts at: 0 263 568 1311 Size: 263 305 743 Fragments arranged by size: 743 305 263 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1289 1311 Size: 1289 22 BtsI GCAGTG_nn' Cuts at: 0 1124 1311 Size: 1124 187 EaeI y'GGCC_r Cuts at: 0 192 1311 Size: 192 1119 EarI CTCTTCn'nnn_ Cuts at: 0 526 568 1028 1311 Size: 526 42 460 283 Fragments arranged by size: 526 460 283 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1276 1311 Size: 1276 35 EcoICRI GAG'CTC Cuts at: 0 549 747 1311 Size: 549 198 564 Fragments arranged by size: 564 549 198 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 565 763 785 1311 Size: 565 198 22 526 Fragments arranged by size: 565 526 198 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 934 1081 1311 Size: 934 147 230 Fragments arranged by size: 934 230 147 EcoO109I rG'GnC_Cy Cuts at: 0 64 336 852 988 989 1311 Size: 64 272 516 136 1 322 Fragments arranged by size: 516 322 272 136 64 1 EcoRI G'AATT_C Cuts at: 0 327 1311 Size: 327 984 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 930 1311 Size: 930 381 HincII GTy'rAC Cuts at: 0 380 1281 1311 Size: 380 901 30 Fragments arranged by size: 901 380 30 HindIII A'AGCT_T Cuts at: 0 1162 1311 Size: 1162 149 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 141 413 539 1179 1311 Size: 141 272 126 640 132 Fragments arranged by size: 640 272 141 132 126 MscI TGG'CCA Cuts at: 0 194 1311 Size: 194 1117 MspA1I CmG'CkG Cuts at: 0 14 265 1311 Size: 14 251 1046 Fragments arranged by size: 1046 251 14 NcoI C'CATG_G Cuts at: 0 568 1311 Size: 568 743 NruI TCG'CGA Cuts at: 0 76 1311 Size: 76 1235 PasI CC'CwG_GG Cuts at: 0 992 1311 Size: 992 319 PflMI CCAn_nnn'nTGG Cuts at: 0 399 456 1311 Size: 399 57 855 Fragments arranged by size: 855 399 57 PfoI T'CCnGG_A Cuts at: 0 424 740 1311 Size: 424 316 571 Fragments arranged by size: 571 424 316 PmlI CAC'GTG Cuts at: 0 1218 1311 Size: 1218 93 PpuMI rG'GwC_Cy Cuts at: 0 336 852 1311 Size: 336 516 459 Fragments arranged by size: 516 459 336 PspOMI G'GGCC_C Cuts at: 0 63 988 1311 Size: 63 925 323 Fragments arranged by size: 925 323 63 PvuII CAG'CTG Cuts at: 0 14 1311 Size: 14 1297 SacI G_AGCT'C Cuts at: 0 551 749 1311 Size: 551 198 562 Fragments arranged by size: 562 551 198 SacII CC_GC'GG Cuts at: 0 266 1311 Size: 266 1045 SapI GCTCTTCn'nnn_ Cuts at: 0 568 1311 Size: 568 743 ScaI AGT'ACT Cuts at: 0 676 1311 Size: 676 635 SexAI A'CCwGG_T Cuts at: 0 1134 1311 Size: 1134 177 SmaI CCC'GGG Cuts at: 0 334 1311 Size: 334 977 SmlI C'TyrA_G Cuts at: 0 790 1306 1311 Size: 790 516 5 Fragments arranged by size: 790 516 5 StuI AGG'CCT Cuts at: 0 805 1311 Size: 805 506 StyI C'CwwG_G Cuts at: 0 568 1311 Size: 568 743 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 841 1311 Size: 841 470 TatI w'GTAC_w Cuts at: 0 674 1101 1311 Size: 674 427 210 Fragments arranged by size: 674 427 210 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1207 1239 1311 Size: 1207 32 72 Fragments arranged by size: 1207 72 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 817 1311 Size: 396 421 494 Fragments arranged by size: 494 421 396 XmaI C'CCGG_G Cuts at: 0 332 1311 Size: 332 979 Enzymes that do cut: AarI AcuI AlwNI ApaI ApoI AvaI BanI BanII BbsI BcgI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HincII HindIII MmeI MscI MspA1I NcoI NruI PasI PflMI PfoI PmlI PpuMI PspOMI PvuII SacI SacII SapI ScaI SexAI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BciVI BclI BfrBI BglI BmtI BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BsrFI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoRV FseI FspI FspAI HaeII Hin4I HpaI KasI KpnI MfeI MluI MslI NaeI NarI NdeI NgoMIV NheI NotI NsiI NspI PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI SanDI SbfI SfcI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI