Restriction map for pFN21AE2285
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# NO CUT ENZYMES (  169 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AclI        
AcsI        AfaI        AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BanI        BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BglI        BglII       BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BseAI       BsiEI       
BsiWI       BsmBI       BsmFI       BsmI        Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrBI       BsrDI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstBI       BstEII      
BstPI       BstUI       BstXI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO65I     EcoRI       EcoRV       EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MaeII       MaeIII      MamI        MfeI        MluI        
MluNI       MroI        MscI        MunI        MvnI        
NaeI        NarI        NciI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsaI        RsrII       
SacI        SacII       SbfI        ScaI        SexAI       
SfcI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        StuI        SwaI        TfiI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XmaCI       XmaI        XmnI        


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# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          190  GTCGAC               GT^[AC][GT]AC
AcyI          364  GACGCC               G[GA]^CG[CT]C
AflII         331  CTTAAG               C^TTAAG
ApaI          311  GGGCCC               GGGCC^C
AspHI         106  GTGCTC               G[AT]GC[AT]^C
AvaI          316  CCCGAG               C^[CT]CG[GA]G
AvaII         432  GGACC                G^G[AT]CC
BanII         311  GGGCCC               G[GA]GC[CT]^C
BbsI           66  GTCTTC               GAAGAC(2/6)
BfrI          331  CTTAAG               C^TTAAG
BpuAI          66  GTCTTC               GAAGAC(2/6)
BsaHI         364  GACGCC               G[GA]^CG[CT]C
BsaI          268  GAGACC               GGTCTC(1/5)
BsaWI           7  TCCGGT               [AT]^CCGG[AT]
BsgI          438  CTGCAC               GTGCAG(16/14)
BsiHKAI       106  GTGCTC               G[AT]GC[AT]^C
BsoBI         316  CCCGAG               C^[CT]CG[GA]G
BstAPI        396  GCATGAATTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I        141  CATCC                GGATG(2/0)
DdeI          118  CTGAG                C^T[ACGT]AG
DraII         311  GGGCCCC              [GA]G^G[ACGT]CC[CT]
EarI          109  CTCTTC               CTCTTC(1/4)
EcoO109I      311  GGGCCCC              [GA]G^G[ACGT]CC[CT]
FokI          141  CATCC                GGATG(9/13)
HapII           8  CCGG                 C^CGG
HgaI          364  GACGC                GACGC(5/10)
Hin1I         364  GACGCC               G[GA]^CG[CT]C
HincII        190  GTCGAC               GT[CT]^[GA]AC
HindII        190  GTCGAC               GT[CT]^[GA]AC
HinfI         319  GAGTC                G^A[ACGT]TC
HpaII           8  CCGG                 C^CGG
HphI           84  GGTGA                GGTGA(8/7)
Ksp632I       109  CTCTTC               CTCTTC(1/4)
MseI          332  TTAA                 T^TAA
MspA1I        435  CCGCTG               C[AC]G^C[GT]G
MspI            8  CCGG                 C^CGG
PleI          319  GAGTC                GAGTC(4/5)
SalI          190  GTCGAC               G^TCGAC
SapI          108  GCTCTTC              GCTCTTC(1/4)
SmlI          331  CTTAAG               C^T[CT][GA]AG
TaqI          191  TCGA                 T^CGA
Tru9I         332  TTAA                 T^TAA
Tsp509I       401  AATT                 ^AATT
TspRI         465  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       191  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (   22 enzymes) #
###################################

BbvI          222  GCTGC                GCAGC(8/12)
              437  GCTGC               
BmyI          106  GTGCTC               G[AGT]GC[ACT]^C
              311  GGGCCC              
BpmI          145  CTGGAG               CTGGAG(16/14)
              481  CTGGAG              
BsiYI         117  CCTGAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              144  CCTGGAGGAGG         
BslI          117  CCTGAGTGAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              144  CCTGGAGGAGG         
BsmAI         268  GAGAC                GTCTC(1/5)
               19  GTCTC               
Bsp1286I      106  GTGCTC               G[AGT]GC[ACT]^C
              311  GGGCCC              
BstYI         161  AGATCC               [GA]^GATC[CT]
              291  GGATCT              
CfoI           93  GCGC                 GCG^C
              325  GCGC                
EcoNI         117  CCTGAGTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
              144  CCTGGAGGAGG         
EcoT14I       272  CCAAGG               C^C[AT][AT]GG
              359  CCAAGG              
HhaI           93  GCGC                 GCG^C
              325  GCGC                
HinP1I         93  GCGC                 G^CGC
              325  GCGC                
MflI          161  AGATCC               [GA]^GATC[CT]
              291  GGATCT              
MslI           33  CATCAAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
               51  CATGAAGGTG          
MwoI          173  GCGATGTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              396  GCATGAATTGC         
NlaIII         51  CATG                 CATG^
              397  CATG                
NlaIV         311  GGGCCC               GG[ACGT]^[ACGT]CC
              312  GGCCCC              
SfaNI         219  GATGC                GCATC(5/9)
              256  GATGC               
StyI          272  CCAAGG               C^C[AT][AT]GG
              359  CCAAGG              
TseI          222  GCTGC                G^C[AT]GC
              437  GCTGC               
XhoII         161  AGATCC               [GA]^GATC[CT]
              291  GGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   22 enzymes) #
#########################################

AciI        AluI        AlwI        BsaJI       BseRI       
BssKI       BstNI       Cac8I       Cfr13I      DpnII       
EcoRII      Fnu4HI      HaeIII      ItaI        MboI        
MboII       MnlI        MvaI        NdeII       Sau3AI      
Sau96I      ScrFI       
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