################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AfaI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HindIII HpaI KasI KpnI KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccI 190 GTCGAC GT^[AC][GT]AC AcyI 364 GACGCC G[GA]^CG[CT]C AflII 331 CTTAAG C^TTAAG ApaI 311 GGGCCC GGGCC^C AspHI 106 GTGCTC G[AT]GC[AT]^C AvaI 316 CCCGAG C^[CT]CG[GA]G AvaII 432 GGACC G^G[AT]CC BanII 311 GGGCCC G[GA]GC[CT]^C BbsI 66 GTCTTC GAAGAC(2/6) BfrI 331 CTTAAG C^TTAAG BpuAI 66 GTCTTC GAAGAC(2/6) BsaHI 364 GACGCC G[GA]^CG[CT]C BsaI 268 GAGACC GGTCTC(1/5) BsaWI 7 TCCGGT [AT]^CCGG[AT] BsgI 438 CTGCAC GTGCAG(16/14) BsiHKAI 106 GTGCTC G[AT]GC[AT]^C BsoBI 316 CCCGAG C^[CT]CG[GA]G BstAPI 396 GCATGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 141 CATCC GGATG(2/0) DdeI 118 CTGAG C^T[ACGT]AG DraII 311 GGGCCCC [GA]G^G[ACGT]CC[CT] EarI 109 CTCTTC CTCTTC(1/4) EcoO109I 311 GGGCCCC [GA]G^G[ACGT]CC[CT] FokI 141 CATCC GGATG(9/13) HapII 8 CCGG C^CGG HgaI 364 GACGC GACGC(5/10) Hin1I 364 GACGCC G[GA]^CG[CT]C HincII 190 GTCGAC GT[CT]^[GA]AC HindII 190 GTCGAC GT[CT]^[GA]AC HinfI 319 GAGTC G^A[ACGT]TC HpaII 8 CCGG C^CGG HphI 84 GGTGA GGTGA(8/7) Ksp632I 109 CTCTTC CTCTTC(1/4) MseI 332 TTAA T^TAA MspA1I 435 CCGCTG C[AC]G^C[GT]G MspI 8 CCGG C^CGG PleI 319 GAGTC GAGTC(4/5) SalI 190 GTCGAC G^TCGAC SapI 108 GCTCTTC GCTCTTC(1/4) SmlI 331 CTTAAG C^T[CT][GA]AG TaqI 191 TCGA T^CGA Tru9I 332 TTAA T^TAA Tsp509I 401 AATT ^AATT TspRI 465 CAGTG CA[GC]TG[ACGT][ACGT]^ TthHB8I 191 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### BbvI 222 GCTGC GCAGC(8/12) 437 GCTGC BmyI 106 GTGCTC G[AGT]GC[ACT]^C 311 GGGCCC BpmI 145 CTGGAG CTGGAG(16/14) 481 CTGGAG BsiYI 117 CCTGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCTGGAGGAGG BslI 117 CCTGAGTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCTGGAGGAGG BsmAI 268 GAGAC GTCTC(1/5) 19 GTCTC Bsp1286I 106 GTGCTC G[AGT]GC[ACT]^C 311 GGGCCC BstYI 161 AGATCC [GA]^GATC[CT] 291 GGATCT CfoI 93 GCGC GCG^C 325 GCGC EcoNI 117 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 144 CCTGGAGGAGG EcoT14I 272 CCAAGG C^C[AT][AT]GG 359 CCAAGG HhaI 93 GCGC GCG^C 325 GCGC HinP1I 93 GCGC G^CGC 325 GCGC MflI 161 AGATCC [GA]^GATC[CT] 291 GGATCT MslI 33 CATCAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 51 CATGAAGGTG MwoI 173 GCGATGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 396 GCATGAATTGC NlaIII 51 CATG CATG^ 397 CATG NlaIV 311 GGGCCC GG[ACGT]^[ACGT]CC 312 GGCCCC SfaNI 219 GATGC GCATC(5/9) 256 GATGC StyI 272 CCAAGG C^C[AT][AT]GG 359 CCAAGG TseI 222 GCTGC G^C[AT]GC 437 GCTGC XhoII 161 AGATCC [GA]^GATC[CT] 291 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AciI AluI AlwI BsaJI BseRI BssKI BstNI Cac8I Cfr13I DpnII EcoRII Fnu4HI HaeIII ItaI MboI MboII MnlI MvaI NdeII Sau3AI Sau96I ScrFI