(Circular) (Six-base) MAPSORT of: pFN21AE2285.seq Check: 1027 from: 1 to: 5203 pFN21AE2285 5203 bp With 182 enzymes: SgfI * August 16, 2010 14:44 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3013 278 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2196 2546 1937 Size: 259 350 4594 Fragments arranged by size: 4594 350 259 Acc65I G'GTAC_C Cuts at: 2524 2524 Size: 5203 AclI AA'CG_TT Cuts at: 1563 3330 3703 1563 Size: 1767 373 3063 Fragments arranged by size: 3063 1767 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3266 4650 4918 1632 Size: 1634 1384 268 1917 Fragments arranged by size: 1917 1634 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2336 820 Size: 197 1319 3687 Fragments arranged by size: 3687 1319 197 AflIII A'CryG_T Cuts at: 1203 4102 5079 1203 Size: 2899 977 1327 Fragments arranged by size: 2899 1327 977 AgeI A'CCGG_T Cuts at: 4034 4034 Size: 5203 AhdI GACnn_n'nnGTC Cuts at: 3932 3932 Size: 5203 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5203 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5203 AlwNI CAG_nnn'CTG Cuts at: 2946 4518 2946 Size: 1572 3631 ApaI G_GGCC'C Cuts at: 2320 2320 Size: 5203 ApaLI G'TGCA_C Cuts at: 3260 4416 3260 Size: 1156 4047 ApoI r'AATT_y Cuts at: 1493 2512 2738 2890 4999 1493 Size: 1019 226 152 2109 1697 Fragments arranged by size: 2109 1697 1019 226 152 AseI AT'TA_AT Cuts at: 160 1032 3757 160 Size: 872 2725 1606 Fragments arranged by size: 2725 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 AvaI C'yCGr_G Cuts at: 1870 1943 2321 2528 1870 Size: 73 378 207 4545 Fragments arranged by size: 4545 378 207 73 BamHI G'GATC_C Cuts at: 2533 2900 2533 Size: 367 4836 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2524 3979 618 Size: 325 217 627 737 1455 1842 Fragments arranged by size: 1842 1455 737 627 325 217 BanII G_rGCy'C Cuts at: 1391 2320 2522 1391 Size: 929 202 4072 Fragments arranged by size: 4072 929 202 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2064 928 Size: 971 165 4067 Fragments arranged by size: 4067 971 165 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3394 3428 1903 Size: 34 1457 34 3678 Fragments arranged by size: 3678 1457 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3095 4305 3095 Size: 1210 3993 BclI T'GATC_A Cuts at: 1548 1548 Size: 5203 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3814 136 Size: 107 122 71 631 605 2142 1525 Fragments arranged by size: 2142 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5198 5198 Size: 5203 BlpI GC'TnA_GC Cuts at: 2620 2620 Size: 5203 Bme1580I G_kGCm'C Cuts at: 1790 2320 3264 4420 1790 Size: 530 944 1156 2573 Fragments arranged by size: 2573 1156 944 530 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5203 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2934 3892 442 Size: 416 53 83 399 24 1517 958 1753 Fragments arranged by size: 1753 1517 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2507 3863 1239 Size: 932 336 1356 2579 Fragments arranged by size: 2579 1356 932 336 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3328 4193 4491 3328 Size: 865 298 4040 Fragments arranged by size: 4040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2267 3866 882 Size: 617 768 1599 2219 Fragments arranged by size: 2219 1599 768 617 BsaAI yAC'GTr Cuts at: 493 1204 2994 493 Size: 711 1790 2702 Fragments arranged by size: 2702 1790 711 BsaBI GATnn'nnATC Cuts at: 5197 5197 Size: 5203 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2370 3010 3392 4027 Size: 53 83 186 936 837 640 382 635 Cuts at: 4027 275 Size: 1451 Fragments arranged by size: 1451 936 837 640 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2012 3636 4034 4308 4455 4866 2012 Size: 1624 398 274 147 411 2349 Fragments arranged by size: 2349 1624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4978 Fragments arranged by size: 4978 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2099 2168 2441 1186 Size: 913 69 273 3948 Fragments arranged by size: 3948 913 273 69 BseYI C'CCAG_C Cuts at: 2461 4406 4902 2461 Size: 1945 496 2762 Fragments arranged by size: 2762 1945 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2428 1163 Size: 573 692 3938 Fragments arranged by size: 3938 692 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2653 3414 4442 1540 Size: 275 186 652 761 1028 2301 Fragments arranged by size: 2301 1028 761 652 275 186 BsiHKAI G_wGCw'C Cuts at: 2115 3264 3349 4420 2115 Size: 1149 85 1071 2898 Fragments arranged by size: 2898 1149 1071 85 BsmI GAATG_Cn' Cuts at: 2714 2807 2714 Size: 93 5110 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2320 2522 3264 3349 4420 1391 Size: 399 325 205 202 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 205 202 85 BspEI T'CCGG_A Cuts at: 4866 4866 Size: 5203 BspHI T'CATG_A Cuts at: 3092 4822 3092 Size: 1730 3473 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2558 844 Size: 1714 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3090 1394 Size: 429 1267 3507 Fragments arranged by size: 3507 1267 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3698 3872 65 Size: 1183 2450 174 1396 Fragments arranged by size: 2450 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3847 4034 1700 Size: 110 2037 187 2869 Fragments arranged by size: 2869 2037 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5203 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5203 BssSI C'ACGA_G Cuts at: 3263 4275 3263 Size: 1012 4191 BstAPI GCAn_nnn'nTGC Cuts at: 794 2407 794 Size: 1613 3590 BstBI TT'CG_AA Cuts at: 4007 4007 Size: 5203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5203 BstYI r'GATC_y Cuts at: 1972 2166 2296 2533 2900 3285 3302 4754 4840 Size: 194 130 237 367 385 17 1452 86 Cuts at: 4840 4852 5198 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 237 194 130 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3712 Fragments arranged by size: 3712 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2193 487 Size: 683 56 967 3497 Fragments arranged by size: 3497 967 683 56 BtsI GCAGTG_nn' Cuts at: 2476 2719 3513 3533 2476 Size: 243 794 20 4146 Fragments arranged by size: 4146 794 243 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5203 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5168 DraI TTT'AAA Cuts at: 2507 2507 Size: 5203 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4210 809 Size: 507 2894 1802 Fragments arranged by size: 2894 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2650 3539 5168 Size: 54 1425 50 275 18 820 889 1629 Cuts at: 5168 8 Size: 43 Fragments arranged by size: 1629 1425 889 820 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2650 1537 Size: 275 838 4090 Fragments arranged by size: 4090 838 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 3133 1353 Size: 767 1013 3423 Fragments arranged by size: 3423 1013 767 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3790 4164 4310 387 Size: 1064 138 2201 374 146 1280 Fragments arranged by size: 2201 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2507 3266 3863 4650 4918 1239 Size: 393 539 336 759 597 787 268 1524 Fragments arranged by size: 1524 787 759 597 539 393 336 268 EcoNI CCTnn'n_nnAGG Cuts at: 2126 2153 2126 Size: 27 5176 EcoO109I rG'GnC_Cy Cuts at: 2317 2317 Size: 5203 EcoRI G'AATT_C Cuts at: 2512 2890 2512 Size: 378 4825 FspI TGC'GCA Cuts at: 3709 3709 Size: 5203 HaeII r_GCGC'y Cuts at: 1678 4350 1678 Size: 2672 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3844 3876 3918 3950 1373 Size: 32 135 32 2272 32 42 32 2626 Fragments arranged by size: 2626 2272 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2197 2547 2793 669 Size: 988 281 259 350 246 3079 Fragments arranged by size: 3079 988 350 281 259 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5203 HpaI GTT'AAC Cuts at: 2793 2793 Size: 5203 KpnI G_GTAC'C Cuts at: 2528 2528 Size: 5203 MfeI C'AATT_G Cuts at: 2802 2802 Size: 5203 MluI A'CGCG_T Cuts at: 5079 5079 Size: 5203 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4317 4501 1305 Size: 3012 184 2007 Fragments arranged by size: 3012 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3381 Fragments arranged by size: 3381 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2042 2060 3161 3520 3679 518 Size: 648 876 18 1101 359 159 2042 Fragments arranged by size: 2042 1101 876 648 359 159 18 MspA1I CmG'CkG Cuts at: 1606 2442 2619 3296 4444 4689 1606 Size: 836 177 677 1148 245 2120 Fragments arranged by size: 2120 1148 836 677 245 177 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3712 Fragments arranged by size: 3712 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5203 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5203 NotI GC'GGCC_GC Cuts at: 2650 2650 Size: 5203 NspI r_CATG'y Cuts at: 1132 1229 2561 4106 1132 Size: 97 1332 1545 2229 Fragments arranged by size: 2229 1545 1332 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5203 PciI A'CATG_T Cuts at: 4102 4102 Size: 5203 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5203 PfoI T'CCnGG_A Cuts at: 2148 2148 Size: 5203 PmeI GTTT'AAAC Cuts at: 2507 2507 Size: 5203 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3272 3304 4785 4817 3272 Size: 32 1481 32 3658 Fragments arranged by size: 3658 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5203 PsiI TTA'TAA Cuts at: 2773 2773 Size: 5203 PspOMI G'GGCC_C Cuts at: 2316 2316 Size: 5203 PstI C_TGCA'G Cuts at: 830 2555 830 Size: 1725 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5203 SalI G'TCGA_C Cuts at: 1936 2195 2545 1936 Size: 259 350 4594 Fragments arranged by size: 4594 350 259 SapI GCTCTTCn'nnn_ Cuts at: 2120 2120 Size: 5203 SbfI CC_TGCA'GG Cuts at: 2555 2555 Size: 5203 ScaI AGT'ACT Cuts at: 3451 5163 3451 Size: 1712 3491 SfcI C'TryA_G Cuts at: 826 1045 2551 3686 4367 4558 826 Size: 219 1506 1135 681 191 1471 Fragments arranged by size: 1506 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5203 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5203 SmaI CCC'GGG Cuts at: 1872 2530 1872 Size: 658 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2336 3307 4208 4470 820 Size: 197 926 393 971 901 262 1553 Fragments arranged by size: 1553 971 926 901 393 262 197 SnaBI TAC'GTA Cuts at: 493 2994 493 Size: 2501 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5203 SphI G_CATG'C Cuts at: 1132 2561 1132 Size: 1429 3774 SspI AAT'ATT Cuts at: 5 52 3127 5 Size: 47 3075 2081 Fragments arranged by size: 3075 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5203 StyI C'CwwG_G Cuts at: 513 1065 2004 2277 2364 2642 513 Size: 552 939 273 87 278 3074 Fragments arranged by size: 3074 939 552 278 273 87 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3230 3247 3400 3585 4030 325 Size: 1255 1650 17 153 185 445 1498 Fragments arranged by size: 1650 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3449 5161 96 Size: 274 80 33 51 1443 1472 1712 138 Fragments arranged by size: 1712 1472 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5163 5195 5163 Size: 32 5171 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5203 XbaI T'CTAG_A Cuts at: 2539 2539 Size: 5203 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5203 XmaI C'CCGG_G Cuts at: 1870 2528 1870 Size: 658 4545 XmnI GAAnn'nnTTC Cuts at: 3332 3332 Size: 5203 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3011 276 Size: 53 83 186 936 1477 2468 Fragments arranged by size: 2468 1477 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI