################################## # NO CUT ENZYMES ( 209 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmAI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MluI MluNI MnlI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TaqI Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AciI 138 CCGC CCGC(-3/-1) BamHI 126 GGATCC G^GATCC BpmI 262 CTGGAG CTGGAG(16/14) BsaJI 130 CCATGG C^C[ACGT][ACGT]GG BsrDI 140 GCAATG GCAATG(2/0) BssKI 261 CCTGG ^CC[ACGT]GG BstNI 261 CCTGG CC^[AT]GG BstXI 6 CCATGAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 126 GGATCC [GA]^GATC[CT] Cac8I 105 GCTGGC GC[ACGT]^[ACGT]GC DdeI 121 CTAAG C^T[ACGT]AG DpnII 127 GATC ^GATC DraI 202 TTTAAA TTT^AAA DsaI 130 CCATGG C^C[GA][CT]GG EcoRII 261 CCTGG ^CC[AT]GG EcoT14I 130 CCATGG C^C[AT][AT]GG HindIII 146 AAGCTT A^AGCTT MboI 127 GATC ^GATC MflI 126 GGATCC [GA]^GATC[CT] MseI 203 TTAA T^TAA MvaI 261 CCTGG CC^[AT]GG MwoI 241 GCTGTAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 130 CCATGG C^CATGG NdeII 127 GATC ^GATC PleI 265 GAGTC GAGTC(4/5) Sau3AI 127 GATC ^GATC ScrFI 261 CCTGG CC^[ACGT]GG SfaNI 288 GCATC GCATC(5/9) SfcI 242 CTGTAG C^T[GA][CT]AG SspI 254 AATATT AAT^ATT StyI 130 CCATGG C^C[AT][AT]GG TfiI 209 GATTC G^A[AT]TC Tru9I 203 TTAA T^TAA XhoII 126 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AlwI 127 GATCC GGATC(4/5) 126 GGATC BbvI 109 GCTGC GCAGC(8/12) 226 GCTGC BsmFI 76 GGGAC GGGAC(10/14) 267 GTCCC Cfr13I 3 GGCCC G^G[ACGT]CC 135 GGGCC HaeIII 3 GGCC GG^CC 136 GGCC HinfI 209 GATTC G^A[ACGT]TC 265 GAGTC MboII 66 GAAGA GAAGA(8/7) 99 GAAGA NlaIV 126 GGATCC GG[ACGT]^[ACGT]CC 134 GGGGCC Sau96I 3 GGCCC G^G[ACGT]CC 135 GGGCC TseI 109 GCTGC G^C[AT]GC 226 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 5 enzymes) # ######################################### AluI Fnu4HI ItaI MslI NlaIII