Restriction map for pFN21AE6146
 (Circular) (Six-base) MAPSORT of: pFN21AE6146.seq  Check: 6937  from: 1  to: 4999



pFN21AE6146  4999 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2809     278 
   Size:         53      83     186     936    1273    2468

  Fragments arranged by size: 

                2468    1273     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2342    1937 
   Size:        405    4594
 
Acc65I G'GTAC_C

Cuts at:   2320    2320 
   Size:       4999
 
AclI AA'CG_TT

Cuts at:   1563    3126    3499    1563 
   Size:       1563     373    3063

  Fragments arranged by size: 

                3063    1563     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3062    4446    4714    1632 
   Size:       1430    1384     268    1917

  Fragments arranged by size: 

                1917    1430    1384     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4802
 
AflIII A'CryG_T

Cuts at:   1203    3898    4875    1203 
   Size:       2695     977    1327

  Fragments arranged by size: 

                2695    1327     977
 
AgeI A'CCGG_T

Cuts at:   3830    3830 
   Size:       4999
 
AhdI GACnn_n'nnGTC

Cuts at:   3728    3728 
   Size:       4999
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4999
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       4999
 
AlwNI CAG_nnn'CTG

Cuts at:   2742    4314    2742 
   Size:       1572    3427
 
ApaLI G'TGCA_C

Cuts at:   3056    4212    3056 
   Size:       1156    3843
 
ApoI r'AATT_y

Cuts at:   1493    2308    2534    2686    4795    1493 
   Size:        815     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     815     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3553     160 
   Size:        872    2521    1606

  Fragments arranged by size: 

                2521    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4999
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2324    1870 
   Size:         73     381    4545

  Fragments arranged by size: 

                4545     381      73
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   2152    2185    2152 
   Size:         33    4966
 
BamHI G'GATC_C

Cuts at:   2131    2329    2696    2131 
   Size:        198     367    4434

  Fragments arranged by size: 

                4434     367     198
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2320    3775     618 
   Size:        325     217     627     533    1455    1842

  Fragments arranged by size: 

                1842    1455     627     533     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2318    1391 
   Size:        927    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    4028
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3190    3224    1903 
   Size:         34    1253      34    3678

  Fragments arranged by size: 

                3678    1253      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2891    4101    2891 
   Size:       1210    3789
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4999
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3610     136 
   Size:        107     122      71     631     605    1938    1525

  Fragments arranged by size: 

                1938    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4994    4994 
   Size:       4999
 
BlpI GC'TnA_GC

Cuts at:   2416    2416 
   Size:       4999
 
Bme1580I G_kGCm'C

Cuts at:   1790    3060    4216    1790 
   Size:       1270    1156    2573

  Fragments arranged by size: 

                2573    1270    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4999
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2730    3688     442 
   Size:        416      53      83     399      24    1313     958    1753

  Fragments arranged by size: 

                1753    1313     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       4999
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2288    3659    1239 
   Size:       1049    1371    2579

  Fragments arranged by size: 

                2579    1371    1049
 
Bpu10I CC'TnA_GC

Cuts at:   2126    2126 
   Size:       4999
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3124    3989    4287    3124 
   Size:        865     298    3836

  Fragments arranged by size: 

                3836     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3662     882 
   Size:        617    2163    2219

  Fragments arranged by size: 

                2219    2163     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2790     493 
   Size:        711    1586    2702

  Fragments arranged by size: 

                2702    1586     711
 
BsaBI GATnn'nnATC

Cuts at:   4993    4993 
   Size:       4999
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2806    3188    3823     275 
   Size:         53      83     186     936    1273     382     635    1451

  Fragments arranged by size: 

                1451    1273     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3432    3830    4104    4251    4662    3432 
   Size:        398     274     147     411    3769

  Fragments arranged by size: 

                3769     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4774

  Fragments arranged by size: 

                4774     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       4999
 
BseYI C'CCAG_C

Cuts at:   4202    4698    4202 
   Size:        496    4503
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4426
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2449    3210    4238    1540 
   Size:        275     186     448     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     448     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3060    3145    4216    3060 
   Size:         85    1071    3843

  Fragments arranged by size: 

                3843    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2510    2603    2510 
   Size:         93    4906
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2318    3060    3145    4216    1391 
   Size:        399     528     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     528     399      85
 
BspEI T'CCGG_A

Cuts at:   4662    4662 
   Size:       4999
 
BspHI T'CATG_A

Cuts at:   2888    4618    2888 
   Size:       1730    3269
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2354     844 
   Size:       1510    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2886    1394 
   Size:        429    1063    3507

  Fragments arranged by size: 

                3507    1063     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2152    3494    3668      65 
   Size:       1183     904    1342     174    1396

  Fragments arranged by size: 

                1396    1342    1183     904     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3643    3830    1700 
   Size:        110    1833     187    2869

  Fragments arranged by size: 

                2869    1833     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4999
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4999
 
BssSI C'ACGA_G

Cuts at:   3059    4071    3059 
   Size:       1012    3987
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       4999
 
BstBI TT'CG_AA

Cuts at:   3803    3803 
   Size:       4999
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4999
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2018    2018 
   Size:       4999
 
BstYI r'GATC_y

Cuts at:   1972    2131    2329    2696    3081    3098    4550    4636    4648 
   Size:        159     198     367     385      17    1452      86      12

Cuts at:   4648    4994    1972 
   Size:        346    1977

  Fragments arranged by size: 

                1977    1452     385     367     346     198     159      86
                  17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2135     513 
   Size:        552     939     131    3377

  Fragments arranged by size: 

                3377     939     552     131
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4260

  Fragments arranged by size: 

                4260     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2515    3309    3329    2515 
   Size:        794      20    4185

  Fragments arranged by size: 

                4185     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4999
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564     529 
   Size:         35    4964
 
DraI TTT'AAA

Cuts at:   2209    2303    2209 
   Size:         94    4905
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4006     809 
   Size:        507    2690    1802

  Fragments arranged by size: 

                2690    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2446    3335    4964 
   Size:         54    1425      50     275      18     616     889    1629

Cuts at:   4964       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425     889     616     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2446    1537 
   Size:        275     634    4090

  Fragments arranged by size: 

                4090     634     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2929    1353 
   Size:       1576    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3586    3960    4106     387 
   Size:       1064     138    1997     374     146    1280

  Fragments arranged by size: 

                1997    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2288    3062    3659    4446    4714    1239 
   Size:        393     656     774     597     787     268    1524

  Fragments arranged by size: 

                1524     787     774     656     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2308    2686    2308 
   Size:        378    4621
 
FspI TGC'GCA

Cuts at:   3505    3505 
   Size:       4999
 
HaeII r_GCGC'y

Cuts at:   1678    4146    1678 
   Size:       2468    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3640    3672    3714    3746    1373 
   Size:         32     135      32    2068      32      42      32    2626

  Fragments arranged by size: 

                2626    2068     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2343    2589     669 
   Size:        988     281     405     246    3079

  Fragments arranged by size: 

                3079     988     405     281     246
 
HindIII A'AGCT_T

Cuts at:    748    2151     748 
   Size:       1403    3596
 
HpaI GTT'AAC

Cuts at:   2589    2589 
   Size:       4999
 
KpnI G_GTAC'C

Cuts at:   2324    2324 
   Size:       4999
 
MfeI C'AATT_G

Cuts at:   2598    2598 
   Size:       4999
 
MluI A'CGCG_T

Cuts at:   4875    4875 
   Size:       4999
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4113    4297    1305 
   Size:       2808     184    2007

  Fragments arranged by size: 

                2808    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3177

  Fragments arranged by size: 

                3177    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2016    2022    2028    2957    3316    3475     518 
   Size:        648     850       6       6     929     359     159    2042

  Fragments arranged by size: 

                2042     929     850     648     359     159       6       6
            
 
MspA1I CmG'CkG

Cuts at:   1606    2415    3092    4240    4485    1606 
   Size:        809     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     809     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    2135     513 
   Size:        552     939     131    3377

  Fragments arranged by size: 

                3377     939     552     131
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4999
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       4999
 
NotI GC'GGCC_GC

Cuts at:   2446    2446 
   Size:       4999
 
NspI r_CATG'y

Cuts at:   1132    1229    2357    3902    1132 
   Size:         97    1128    1545    2229

  Fragments arranged by size: 

                2229    1545    1128      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4999
 
PciI A'CATG_T

Cuts at:   3898    3898 
   Size:       4999
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4999
 
PmeI GTTT'AAAC

Cuts at:   2303    2303 
   Size:       4999
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3068    3100    4581    4613    3068 
   Size:         32    1481      32    3454

  Fragments arranged by size: 

                3454    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       4999
 
PsiI TTA'TAA

Cuts at:   2569    2569 
   Size:       4999
 
PstI C_TGCA'G

Cuts at:    830    2351     830 
   Size:       1521    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4999
 
SalI G'TCGA_C

Cuts at:   1936    2341    1936 
   Size:        405    4594
 
SbfI CC_TGCA'GG

Cuts at:   2351    2351 
   Size:       4999
 
ScaI AGT'ACT

Cuts at:   3247    4959    3247 
   Size:       1712    3287
 
SfcI C'TryA_G

Cuts at:    826    1045    2247    2347    3482    4163    4354     826 
   Size:        219    1202     100    1135     681     191    1471

  Fragments arranged by size: 

                1471    1202    1135     681     219     191     100
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4999
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4999
 
SmaI CCC'GGG

Cuts at:   1872    2326    1872 
   Size:        454    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3103    4004    4266     820 
   Size:        197     926    1160     901     262    1553

  Fragments arranged by size: 

                1553    1160     926     901     262     197
 
SnaBI TAC'GTA

Cuts at:    493    2790     493 
   Size:       2297    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4999
 
SphI G_CATG'C

Cuts at:   1132    2357    1132 
   Size:       1225    3774
 
SspI AAT'ATT

Cuts at:      5      52    2261    2923       5 
   Size:         47    2209     662    2081

  Fragments arranged by size: 

                2209    2081     662      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4999
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2135    2438     513 
   Size:        552     939     131     303    3074

  Fragments arranged by size: 

                3074     939     552     303     131
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2156    3026    3043    3196    3381    3826     325 
   Size:       1255     576     870      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     870     576     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3245    4957      96 
   Size:        274      80      33      51    1443    1268    1712     138

  Fragments arranged by size: 

                1712    1443    1268     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4959    4991    4959 
   Size:         32    4967
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4999
 
XbaI T'CTAG_A

Cuts at:   2335    2335 
   Size:       4999
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4999
 
XmaI C'CCGG_G

Cuts at:   1870    2324    1870 
   Size:        454    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3128    3128 
   Size:       4999
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2807     276 
   Size:         53      83     186     936    1273    2468

  Fragments arranged by size: 

                2468    1273     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII
     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I
     NcoI     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI
     PmeI     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI
     ScaI     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XbaI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BbeI    BbvCI
    BfrBI     BplI    BsiWI    BsmBI  BstZ17I   Bsu36I   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI     KasI     NaeI
     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI    PpuMI
   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII    SanDI
     SapI    SexAI     SfiI     SfoI     SrfI     SwaI     XcmI
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