################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AcyI AflII AflIII AgeI AhdI AlwI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrBI BsrFI BsrGI BsrI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AccII 388 CGCG CG^CG AccIII 488 TCCGGA T^CCGGA AfaI 141 GTAC GT^AC Alw44I 462 GTGCAC G^TGCAC AlwNI 328 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 462 GTGCAC G^TGCAC AscI 386 GGCGCGCC GG^CGCGCC AvaI 362 CCCGGG C^[CT]CG[GA]G AviII 269 TGCGCA TGC^GCA BalI 596 TGGCCA TGG^CCA BpmI 586 CTGGAG CTGGAG(16/14) BsaI 37 GAGACC GGTCTC(1/5) BsaWI 488 TCCGGA [AT]^CCGG[AT] BseAI 488 TCCGGA T^CCGGA BseRI 549 CTCCTC GAGGAG(10/8) BsiEI 237 CGGCCG CG[GA][CT]^CG BsiYI 321 CCCTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 321 CCCTCTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmBI 402 GAGACG CGTCTC(1/5) BsoBI 362 CCCGGG C^[CT]CG[GA]G BspEI 488 TCCGGA T^CCGGA BspHI 260 TCATGA T^CATGA BssHII 387 GCGCGC G^CGCGC BssSI 473 CTCGTG CACGAG(-5/-1) BstF5I 417 GGATG GGATG(2/0) BstUI 388 CGCG CG^CG DraII 329 AGGTCCT [GA]G^G[ACGT]CC[CT] EagI 237 CGGCCG C^GGCCG EclXI 237 CGGCCG C^GGCCG Eco52I 237 CGGCCG C^GGCCG EcoO109I 329 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 542 CCAAGG C^C[AT][AT]GG FokI 417 GGATG GGATG(9/13) FspI 269 TGCGCA TGC^GCA HinfI 370 GACTC G^A[ACGT]TC HphI 336 GGTGA GGTGA(8/7) MboII 573 GAAGA GAAGA(8/7) MluNI 596 TGGCCA TGG^CCA MroI 488 TCCGGA T^CCGGA MscI 596 TGGCCA TGG^CCA MspA1I 26 CAGCGG C[AC]G^C[GT]G MvnI 388 CGCG CG^CG NspI 282 GCATGC [GA]CATG^[CT] PleI 370 GACTC GAGTC(4/5) PpuMI 329 AGGTCCT [GA]G^G[AT]CC[CT] RcaI 260 TCATGA T^CATGA RsaI 141 GTAC GT^AC SacI 526 GAGCTC GAGCT^C SbfI 102 CCTGCAGG CCTGCA^GG SmaI 362 CCCGGG CCC^GGG SphI 282 GCATGC GCATG^C Sse8387I 102 CCTGCAGG CCTGCA^GG StyI 542 CCAAGG C^C[AT][AT]GG XmaCI 362 CCCGGG C^CCGGG XmaI 362 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AvaII 330 GGTCC G^G[AT]CC 358 GGTCC BanII 526 GAGCTC G[GA]GC[CT]^C 546 GGGCTC BsgI 82 GTGCAG GTGCAG(16/14) 155 GTGCAG BsrDI 114 CATTGC GCAATG(2/0) 504 CATTGC Cac8I 282 GCATGC GC[ACGT]^[ACGT]GC 387 GCGCGC Cfr13I 330 GGTCC G^G[ACGT]CC 358 GGTCC DdeI 191 CTCAG C^T[ACGT]AG 509 CTGAG DpnII 87 GATC ^GATC 294 GATC EaeI 237 CGGCCG [CT]^GGCC[GA] 596 TGGCCA HaeII 209 AGCGCC [GA]GCGC^[CT] 469 GGCGCT MboI 87 GATC ^GATC 294 GATC MseI 497 TTAA T^TAA 569 TTAA NdeII 87 GATC ^GATC 294 GATC NlaIV 358 GGTCCC GG[ACGT]^[ACGT]CC 547 GGCTCC PstI 103 CTGCAG CTGCA^G 325 CTGCAG Sau3AI 87 GATC ^GATC 294 GATC Sau96I 330 GGTCC G^G[ACGT]CC 358 GGTCC SfaNI 231 GATGC GCATC(5/9) 272 GCATC SfcI 103 CTGCAG C^T[GA][CT]AG 325 CTGCAG TaqI 290 TCGA T^CGA 553 TCGA Tru9I 497 TTAA T^TAA 569 TTAA TthHB8I 290 TCGA T^CGA 553 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 33 enzymes) # ######################################### AciI AluI AspHI BbvI BcnI BmyI BsaJI BsiHKAI BsmAI BsmFI Bsp1286I BspMI BssKI BstNI CfoI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MnlI MslI MspI MvaI MwoI NciI NlaIII ScrFI TseI TspRI