Restriction map for
pFN21AE5217
(Circular) (Six-base) MAPSORT of: pFN21AE5217.seq Check: 2459 from: 1 to: 5317
pFN21AE5217 5317 bp
With 182 enzymes: SgfI *
January 30, 2014 17:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2316 2316
Size: 5317
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3127 278
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2660 1937
Size: 723 4594
Acc65I G'GTAC_C
Cuts at: 2638 2638
Size: 5317
AclI AA'CG_TT
Cuts at: 1563 3444 3817 1563
Size: 1881 373 3063
Fragments arranged by size:
3063 1881 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3380 4764 5032 1632
Size: 1748 1384 268 1917
Fragments arranged by size:
1917 1748 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5120
AflIII A'CryG_T
Cuts at: 1203 4216 5193 1203
Size: 3013 977 1327
Fragments arranged by size:
3013 1327 977
AgeI A'CCGG_T
Cuts at: 4148 4148
Size: 5317
AhdI GACnn_n'nnGTC
Cuts at: 4046 4046
Size: 5317
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5317
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5317
AlwNI CAG_nnn'CTG
Cuts at: 2338 3060 4632 2338
Size: 722 1572 3023
Fragments arranged by size:
3023 1572 722
ApaLI G'TGCA_C
Cuts at: 2467 3374 4530 2467
Size: 907 1156 3254
Fragments arranged by size:
3254 1156 907
ApoI r'AATT_y
Cuts at: 1493 2626 2852 3004 5113 1493
Size: 1133 226 152 2109 1697
Fragments arranged by size:
2109 1697 1133 226 152
AscI GG'CGCG_CC
Cuts at: 2392 2392
Size: 5317
AseI AT'TA_AT
Cuts at: 160 1032 3871 160
Size: 872 2839 1606
Fragments arranged by size:
2839 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
AvaI C'yCGr_G
Cuts at: 1870 1943 2367 2642 1870
Size: 73 424 275 4545
Fragments arranged by size:
4545 424 275 73
BamHI G'GATC_C
Cuts at: 2647 3014 2647
Size: 367 4950
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2638 4093 618
Size: 325 217 627 851 1455 1842
Fragments arranged by size:
1842 1455 851 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2535 2555 2636 1391
Size: 1144 20 81 4072
Fragments arranged by size:
4072 1144 81 20
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4346
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3508 3542 1903
Size: 34 1571 34 3678
Fragments arranged by size:
3678 1571 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3209 4419 3209
Size: 1210 4107
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5317
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3928 136
Size: 107 122 71 631 605 2256 1525
Fragments arranged by size:
2256 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5312 5312
Size: 5317
BlpI GC'TnA_GC
Cuts at: 2734 2734
Size: 5317
Bme1580I G_kGCm'C
Cuts at: 1790 2471 3378 4534 1790
Size: 681 907 1156 2573
Fragments arranged by size:
2573 1156 907 681
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5317
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3048 4006 442
Size: 416 53 83 399 24 1631 958 1753
Fragments arranged by size:
1753 1631 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2612 3977 1239
Size: 1373 1365 2579
Fragments arranged by size:
2579 1373 1365
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3442 4307 4605 3442
Size: 865 298 4154
Fragments arranged by size:
4154 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2036 3980 882
Size: 617 537 1944 2219
Fragments arranged by size:
2219 1944 617 537
BsaAI yAC'GTr
Cuts at: 493 1204 3108 493
Size: 711 1904 2702
Fragments arranged by size:
2702 1904 711
BsaBI GATnn'nnATC
Cuts at: 5311 5311
Size: 5317
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3124 3506 4141 275
Size: 53 83 186 936 1591 382 635 1451
Fragments arranged by size:
1591 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2493 3750 4148 4422 4569 4980 2493
Size: 1257 398 274 147 411 2830
Fragments arranged by size:
2830 1257 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5092
Fragments arranged by size:
5092 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2545 1186
Size: 1359 3958
BseYI C'CCAG_C
Cuts at: 4520 5016 4520
Size: 496 4821
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2108 2181 1163
Size: 573 372 73 4299
Fragments arranged by size:
4299 573 372 73
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2245 2767 3528 4556 1540
Size: 275 186 244 522 761 1028 2301
Fragments arranged by size:
2301 1028 761 522 275 244 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2471 2535 3378 3463 4534 2078
Size: 393 64 843 85 1071 2861
Fragments arranged by size:
2861 1071 843 393 85 64
BsmI GAATG_Cn'
Cuts at: 2828 2921 2828
Size: 93 5224
BsmBI CGTCTCn'nnnn_
Cuts at: 2401 2401
Size: 5317
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2471 2535 2555 2636 3378 3463
Size: 399 288 393 64 20 81 742 85
Cuts at: 3463 4534 1391
Size: 1071 2174
Fragments arranged by size:
2174 1071 742 399 393 288 85 81
64 20
BspEI T'CCGG_A
Cuts at: 2493 4980 2493
Size: 2487 2830
BspHI T'CATG_A
Cuts at: 2265 3206 4936 2265
Size: 941 1730 2646
Fragments arranged by size:
2646 1730 941
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2157 2316 2323 2672 844
Size: 1313 159 7 349 3489
Fragments arranged by size:
3489 1313 349 159 7
BsrBI CCG'CTC
Cuts at: 1394 1823 3204 1394
Size: 429 1381 3507
Fragments arranged by size:
3507 1381 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2118 2508 3812 3986 65
Size: 1183 870 390 1304 174 1396
Fragments arranged by size:
1396 1304 1183 870 390 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3961 4148 1700
Size: 110 2151 187 2869
Fragments arranged by size:
2869 2151 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5317
BssHII G'CGCG_C
Cuts at: 1925 2392 1925
Size: 467 4850
BssSI C'ACGA_G
Cuts at: 2478 3377 4389 2478
Size: 899 1012 3406
Fragments arranged by size:
3406 1012 899
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5317
BstBI TT'CG_AA
Cuts at: 4121 4121
Size: 5317
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5317
BstYI r'GATC_y
Cuts at: 1972 2647 3014 3399 3416 4868 4954 4966 5312
Size: 675 367 385 17 1452 86 12 346
Cuts at: 5312 1972
Size: 1977
Fragments arranged by size:
1977 1452 675 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 4765
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4578
Fragments arranged by size:
4578 683 56
BtsI GCAGTG_nn'
Cuts at: 2130 2833 3627 3647 2130
Size: 703 794 20 3800
Fragments arranged by size:
3800 794 703 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5317
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2368 2403 529
Size: 35 1804 35 3443
Fragments arranged by size:
3443 1804 35 35
DraI TTT'AAA
Cuts at: 2621 2621
Size: 5317
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4324 809
Size: 507 3008 1802
Fragments arranged by size:
3008 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2242 2601 2764
Size: 54 1425 50 275 18 412 359 163
Cuts at: 2764 3653 5282 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 412 359 275 163 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2242 2764 1537
Size: 275 430 522 4090
Fragments arranged by size:
4090 522 430 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3247 1353
Size: 1894 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3904 4278 4424 387
Size: 1064 138 2315 374 146 1280
Fragments arranged by size:
2315 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2533 2533
Size: 5317
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2612 3380 3977 4764 5032 1239
Size: 393 980 768 597 787 268 1524
Fragments arranged by size:
1524 980 787 768 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2335 2335
Size: 5317
EcoRI G'AATT_C
Cuts at: 2626 3004 2626
Size: 378 4939
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2308 2308
Size: 5317
FspI TGC'GCA
Cuts at: 2276 3823 2276
Size: 1547 3770
FspAI rTGC'GCAy
Cuts at: 2276 2276
Size: 5317
HaeII r_GCGC'y
Cuts at: 1678 2218 2478 4464 1678
Size: 540 260 1986 2531
Fragments arranged by size:
2531 1986 540 260
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3958 3990 4032 4064 1373
Size: 32 135 32 2386 32 42 32 2626
Fragments arranged by size:
2626 2386 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2661 2907 669
Size: 988 281 723 246 3079
Fragments arranged by size:
3079 988 723 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5317
HpaI GTT'AAC
Cuts at: 2907 2907
Size: 5317
KpnI G_GTAC'C
Cuts at: 2642 2642
Size: 5317
MfeI C'AATT_G
Cuts at: 2916 2916
Size: 5317
MluI A'CGCG_T
Cuts at: 5193 5193
Size: 5317
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2634 4431 4615 1305
Size: 1329 1797 184 2007
Fragments arranged by size:
2007 1797 1329 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2603 10
Size: 54 1425 343 771 2724
Fragments arranged by size:
2724 1425 771 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2123 2234 2270 3275 3634 3793 518
Size: 648 957 111 36 1005 359 159 2042
Fragments arranged by size:
2042 1005 957 648 359 159 111 36
MspA1I CmG'CkG
Cuts at: 1606 2033 2733 3410 4558 4803 1606
Size: 427 700 677 1148 245 2120
Fragments arranged by size:
2120 1148 700 677 427 245
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 4765
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5317
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5317
NotI GC'GGCC_GC
Cuts at: 2764 2764
Size: 5317
NspI r_CATG'y
Cuts at: 1132 1229 2291 2675 4220 1132
Size: 97 1062 384 1545 2229
Fragments arranged by size:
2229 1545 1062 384 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5317
PciI A'CATG_T
Cuts at: 4216 4216
Size: 5317
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5317
PmeI GTTT'AAAC
Cuts at: 2621 2621
Size: 5317
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3386 3418 4899 4931 3386
Size: 32 1481 32 3772
Fragments arranged by size:
3772 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2335 2335
Size: 5317
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5317
PsiI TTA'TAA
Cuts at: 2887 2887
Size: 5317
PstI C_TGCA'G
Cuts at: 830 2112 2334 2669 830
Size: 1282 222 335 3478
Fragments arranged by size:
3478 1282 335 222
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5317
SacI G_AGCT'C
Cuts at: 2535 2535
Size: 5317
SalI G'TCGA_C
Cuts at: 1936 2659 1936
Size: 723 4594
SbfI CC_TGCA'GG
Cuts at: 2112 2669 2112
Size: 557 4760
ScaI AGT'ACT
Cuts at: 3565 5277 3565
Size: 1712 3605
SfcI C'TryA_G
Cuts at: 826 1045 2108 2330 2665 3800 4481 4672 826
Size: 219 1063 222 335 1135 681 191 1471
Fragments arranged by size:
1471 1135 1063 681 335 222 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5317
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5317
SmaI CCC'GGG
Cuts at: 1872 2369 2644 1872
Size: 497 275 4545
Fragments arranged by size:
4545 497 275
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3421 4322 4584 820
Size: 197 926 1478 901 262 1553
Fragments arranged by size:
1553 1478 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3108 493
Size: 2615 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5317
SphI G_CATG'C
Cuts at: 1132 2291 2675 1132
Size: 1159 384 3774
Fragments arranged by size:
3774 1159 384
SspI AAT'ATT
Cuts at: 5 52 3241 5
Size: 47 3189 2081
Fragments arranged by size:
3189 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5317
StyI C'CwwG_G
Cuts at: 513 1065 2547 2756 513
Size: 552 1482 209 3074
Fragments arranged by size:
3074 1482 552 209
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2024 3344 3361 3514 3699 4144 325
Size: 1255 444 1320 17 153 185 445 1498
Fragments arranged by size:
1498 1320 1255 445 444 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3563 5275 96
Size: 274 80 33 51 1443 1586 1712 138
Fragments arranged by size:
1712 1586 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5277 5309 5277
Size: 32 5285
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5317
XbaI T'CTAG_A
Cuts at: 2653 2653
Size: 5317
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5317
XmaI C'CCGG_G
Cuts at: 1870 2367 2642 1870
Size: 497 275 4545
Fragments arranged by size:
4545 497 275
XmnI GAAnn'nnTTC
Cuts at: 3446 3446
Size: 5317
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3125 276
Size: 53 83 186 936 1591 2468
Fragments arranged by size:
2468 1591 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI
BtsI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NcoI NdeI NheI NotI
NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI
PsiI PstI PvuI SacI SalI SbfI ScaI SfcI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI
StuI StyI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI
EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI
PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII
SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI
XcmI