(Linear) (Six-base) MAPSORT of: KIEE5217.seq Check: 6134 from: 1 to: 612 KIEE5217.orf With 182 enzymes: SgfI * January 29, 2014 11:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 311 612 Size: 311 301 AlwNI CAG_nnn'CTG Cuts at: 0 333 612 Size: 333 279 ApaLI G'TGCA_C Cuts at: 0 462 612 Size: 462 150 AscI GG'CGCG_CC Cuts at: 0 387 612 Size: 387 225 AvaI C'yCGr_G Cuts at: 0 362 612 Size: 362 250 BanII G_rGCy'C Cuts at: 0 530 550 612 Size: 530 20 62 Fragments arranged by size: 530 62 20 Bme1580I G_kGCm'C Cuts at: 0 466 612 Size: 466 146 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 612 Size: 607 5 BsaI GGTCTCn'nnnn_ Cuts at: 0 31 612 Size: 31 581 BsaWI w'CCGG_w Cuts at: 0 488 612 Size: 488 124 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 540 612 Size: 540 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 103 176 612 Size: 103 73 436 Fragments arranged by size: 436 103 73 BsiEI CG_ry'CG Cuts at: 0 240 612 Size: 240 372 BsiHKAI G_wGCw'C Cuts at: 0 73 466 530 612 Size: 73 393 64 82 Fragments arranged by size: 393 82 73 64 BsmBI CGTCTCn'nnnn_ Cuts at: 0 396 612 Size: 396 216 Bsp1286I G_dGCh'C Cuts at: 0 73 466 530 550 612 Size: 73 393 64 20 62 Fragments arranged by size: 393 73 64 62 20 BspEI T'CCGG_A Cuts at: 0 488 612 Size: 488 124 BspHI T'CATG_A Cuts at: 0 260 612 Size: 260 352 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 152 311 318 612 Size: 152 159 7 294 Fragments arranged by size: 294 159 152 7 BsrDI GCAATG_nn' Cuts at: 0 113 503 612 Size: 113 390 109 Fragments arranged by size: 390 113 109 BssHII G'CGCG_C Cuts at: 0 387 612 Size: 387 225 BssSI C'ACGA_G Cuts at: 0 473 612 Size: 473 139 BtsI GCAGTG_nn' Cuts at: 0 125 612 Size: 125 487 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 363 398 612 Size: 363 35 214 Fragments arranged by size: 363 214 35 EaeI y'GGCC_r Cuts at: 0 237 596 612 Size: 237 359 16 Fragments arranged by size: 359 237 16 EagI C'GGCC_G Cuts at: 0 237 612 Size: 237 375 EcoICRI GAG'CTC Cuts at: 0 528 612 Size: 528 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 607 612 Size: 607 5 EcoO109I rG'GnC_Cy Cuts at: 0 330 612 Size: 330 282 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 303 612 Size: 303 309 FspI TGC'GCA Cuts at: 0 271 612 Size: 271 341 FspAI rTGC'GCAy Cuts at: 0 271 612 Size: 271 341 HaeII r_GCGC'y Cuts at: 0 213 473 612 Size: 213 260 139 Fragments arranged by size: 260 213 139 MscI TGG'CCA Cuts at: 0 598 612 Size: 598 14 MslI CAynn'nnrTG Cuts at: 0 118 229 265 612 Size: 118 111 36 347 Fragments arranged by size: 347 118 111 36 MspA1I CmG'CkG Cuts at: 0 28 612 Size: 28 584 NspI r_CATG'y Cuts at: 0 286 612 Size: 286 326 PpuMI rG'GwC_Cy Cuts at: 0 330 612 Size: 330 282 PstI C_TGCA'G Cuts at: 0 107 329 612 Size: 107 222 283 Fragments arranged by size: 283 222 107 SacI G_AGCT'C Cuts at: 0 530 612 Size: 530 82 SbfI CC_TGCA'GG Cuts at: 0 107 612 Size: 107 505 SfcI C'TryA_G Cuts at: 0 103 325 612 Size: 103 222 287 Fragments arranged by size: 287 222 103 SmaI CCC'GGG Cuts at: 0 364 612 Size: 364 248 SphI G_CATG'C Cuts at: 0 286 612 Size: 286 326 StyI C'CwwG_G Cuts at: 0 542 612 Size: 542 70 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 19 612 Size: 19 593 XmaI C'CCGG_G Cuts at: 0 362 612 Size: 362 250 Enzymes that do cut: AarI AlwNI ApaLI AscI AvaI BanII Bme1580I BpmI BsaI BsaWI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BssHII BssSI BtsI CspCI EaeI EagI EcoICRI Eco57MI EcoO109I FalI FspI FspAI HaeII MscI MslI MspA1I NspI PpuMI PstI SacI SbfI SfcI SmaI SphI StyI TaqII XmaI Enzymes that do not cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApoI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaXI BseYI BsiWI BsmI BsrBI BsrFI BsrGI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI DraI DraIII DrdI EarI EciI EcoNI EcoRI EcoRV FseI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI StuI SwaI TatI TstI Tth111I XbaI XcmI XhoI XmnI ZraI