################################## # NO CUT ENZYMES ( 190 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AcyI AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BbvI BcgI BciVI BcnI BfaI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I Cfr13I ClaI CpoI DdeI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI Fnu4HI FseI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeI MaeIII MflI MluI MluNI MroI MscI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI TseI Tsp45I Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AclI 112 AACGTT AA^CGTT AcsI 343 GAATTC [GA]^AATT[CT] AflII 667 CTTAAG C^TTAAG ApoI 343 GAATTC [GA]^AATT[CT] BbsI 634 GAAGAC GAAGAC(2/6) BclI 72 TGATCA T^GATCA BfrI 667 CTTAAG C^TTAAG BpuAI 634 GAAGAC GAAGAC(2/6) BsaBI 67 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 247 GGTCTC GGTCTC(1/5) BsaJI 656 CCTTGG C^C[ACGT][ACGT]GG BsiEI 447 CGATCG CG[GA][CT]^CG BsiYI 527 CCAAAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 527 CCAAAAGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 360 GGGAC GGGAC(10/14) BspHI 683 TCATGA T^CATGA BsrI 363 ACTGG ACTGG(1/-1) Cac8I 533 GCAGGC GC[ACGT]^[ACGT]GC EcoRI 343 GAATTC G^AATTC EcoT14I 656 CCTTGG C^C[AT][AT]GG FbaI 72 TGATCA T^GATCA HinfI 467 GAATC G^A[ACGT]TC MaeII 113 ACGT A^CGT MamI 67 GATACTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 743 CAATTG C^AATTG MunI 743 CAATTG C^AATTG MwoI 331 GCACAAGAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspI 261 GCATGT [GA]CATG^[CT] Psp1406I 112 AACGTT AA^CGTT PvuI 447 CGATCG CGAT^CG RcaI 683 TCATGA T^CATGA StyI 656 CCTTGG C^C[AT][AT]GG SwaI 585 ATTTAAAT ATTT^AAAT TfiI 467 GAATC G^A[AT]TC XcmI 527 CCAAAAGCAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AluI 353 AGCT AG^CT 401 AGCT Asp700I 304 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 343 GAATTCTTTC BseRI 250 CTCCTC GAGGAG(10/8) 553 GAGGAG DpnII 73 GATC ^GATC 448 GATC DraI 586 TTTAAA TTT^AAA 693 TTTAAA EarI 253 CTCTTC CTCTTC(1/4) 20 GAAGAG Ksp632I 253 CTCTTC CTCTTC(1/4) 20 GAAGAG MboI 73 GATC ^GATC 448 GATC MslI 96 CACACTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 751 CACAGCGATG NdeII 73 GATC ^GATC 448 GATC NlaIII 262 CATG CATG^ 684 CATG Sau3AI 73 GATC ^GATC 448 GATC SfaNI 214 GATGC GCATC(5/9) 757 GATGC SmlI 355 CTCAAG C^T[CT][GA]AG 667 CTTAAG TaqI 312 TCGA T^CGA 618 TCGA Tsp509I 344 AATT ^AATT 744 AATT TspRI 98 CACTG CA[GC]TG[ACGT][ACGT]^ 149 CACTG TthHB8I 312 TCGA T^CGA 618 TCGA XmnI 304 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 343 GAATTCTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 14 enzymes) # ######################################### AfaI BsmAI BssKI BstF5I BstNI EcoRII FokI MboII MnlI MseI MvaI RsaI ScrFI Tru9I