Restriction map for pFN21AE3944
 (Circular) (Six-base) MAPSORT of: pFN21AE3944.seq  Check: 51  from: 1  to: 5482



pFN21AE3944  5482 bp

 With 182 enzymes: SgfI * 

                        March 28, 2012 11:49  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    3292     278 
   Size:         53      83     186     936    1756    2468

  Fragments arranged by size: 

                2468    1756     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2825    1937 
   Size:        888    4594
 
Acc65I G'GTAC_C

Cuts at:   2803    2803 
   Size:       5482
 
AclI AA'CG_TT

Cuts at:   1563    2118    3609    3982    1563 
   Size:        555    1491     373    3063

  Fragments arranged by size: 

                3063    1491     555     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2245    3545    4929    5197    1632 
   Size:        613    1300    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1300     613     268
 
AflII C'TTAA_G

Cuts at:    820    1017    2672     820 
   Size:        197    1655    3630

  Fragments arranged by size: 

                3630    1655     197
 
AflIII A'CryG_T

Cuts at:   1203    4381    5358    1203 
   Size:       3178     977    1327

  Fragments arranged by size: 

                3178    1327     977
 
AgeI A'CCGG_T

Cuts at:   4313    4313 
   Size:       5482
 
AhdI GACnn_n'nnGTC

Cuts at:   4211    4211 
   Size:       5482
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       5482
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       5482
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2081    2113    2081 
   Size:         32    5450
 
AlwNI CAG_nnn'CTG

Cuts at:   3225    4797    3225 
   Size:       1572    3910
 
ApaLI G'TGCA_C

Cuts at:   3539    4695    3539 
   Size:       1156    4326
 
ApoI r'AATT_y

Cuts at:   1493    2348    2791    3017    3169    5278    1493 
   Size:        855     443     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     855     443     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    4036     160 
   Size:        872    3004    1606

  Fragments arranged by size: 

                3004    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5482
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2807    1870 
   Size:         73     864    4545

  Fragments arranged by size: 

                4545     864      73
 
BamHI G'GATC_C

Cuts at:   2812    3179    2812 
   Size:        367    5115
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2803    4258     618 
   Size:        325     217     627    1016    1455    1842

  Fragments arranged by size: 

                1842    1455    1016     627     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2801    1391 
   Size:       1410    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2646     928 
   Size:        971     747    3764

  Fragments arranged by size: 

                3764     971     747
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3673    3707    1903 
   Size:         34    1736      34    3678

  Fragments arranged by size: 

                3678    1736      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   3374    4584    3374 
   Size:       1210    4272
 
BclI T'GATC_A

Cuts at:   1548    2077    1548 
   Size:        529    4953
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4093     136 
   Size:        107     122      71     631     605    2421    1525

  Fragments arranged by size: 

                2421    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   5477    5477 
   Size:       5482
 
BlpI GC'TnA_GC

Cuts at:   2899    2899 
   Size:       5482
 
Bme1580I G_kGCm'C

Cuts at:   1790    3543    4699    1790 
   Size:       1753    1156    2573

  Fragments arranged by size: 

                2573    1753    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       5482
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3213    4171     442 
   Size:        416      53      83     399      24    1796     958    1753

  Fragments arranged by size: 

                1796    1753     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    1056 
   Size:       5482
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    4142    1239 
   Size:       2903    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2345    3607    4472    4770    2345 
   Size:       1262     865     298    3057

  Fragments arranged by size: 

                3057    1262     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    2258    4145     882 
   Size:        617     759    1887    2219

  Fragments arranged by size: 

                2219    1887     759     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    3273     493 
   Size:        711    2069    2702

  Fragments arranged by size: 

                2702    2069     711
 
BsaBI GATnn'nnATC

Cuts at:   2076    5476    2076 
   Size:       3400    2082
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    3289    3671    4306     275 
   Size:         53      83     186     936    1756     382     635    1451

  Fragments arranged by size: 

                1756    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3915    4313    4587    4734    5145    3915 
   Size:        398     274     147     411    4252

  Fragments arranged by size: 

                4252     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    5257

  Fragments arranged by size: 

                5257     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2246    2573    1186 
   Size:       1060     327    4095

  Fragments arranged by size: 

                4095    1060     327
 
BseYI C'CCAG_C

Cuts at:   4685    5181    4685 
   Size:        496    4986
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4909
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2455    2932    3693    4721    1540 
   Size:        275     186     454     477     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     477     454     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3543    3628    4699    3543 
   Size:         85    1071    4326

  Fragments arranged by size: 

                4326    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2993    3086    2993 
   Size:         93    5389
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2801    3543    3628    4699    1391 
   Size:        399    1011     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071    1011     742     399      85
 
BspEI T'CCGG_A

Cuts at:   5145    5145 
   Size:       5482
 
BspHI T'CATG_A

Cuts at:   2688    3371    5101    2688 
   Size:        683    1730    3069

  Fragments arranged by size: 

                3069    1730     683
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2837     844 
   Size:       1993    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    3369    1394 
   Size:        429    1546    3507

  Fragments arranged by size: 

                3507    1546     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3977    4151      65 
   Size:       1183    2729     174    1396

  Fragments arranged by size: 

                2729    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    4126    4313    1700 
   Size:        110    2316     187    2869

  Fragments arranged by size: 

                2869    2316     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       5482
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       5482
 
BssSI C'ACGA_G

Cuts at:   3542    4554    3542 
   Size:       1012    4470
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       5482
 
BstBI TT'CG_AA

Cuts at:   4286    4286 
   Size:       5482
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       5482
 
BstYI r'GATC_y

Cuts at:   1972    2812    3179    3564    3581    5033    5119    5131    5477 
   Size:        840     367     385      17    1452      86      12     346

Cuts at:   5477    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     840     385     367     346      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3991

  Fragments arranged by size: 

                3991     939     552
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2775     487 
   Size:        683      56    1549    3194

  Fragments arranged by size: 

                3194    1549     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2998    3792    3812    2998 
   Size:        794      20    4668

  Fragments arranged by size: 

                4668     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       5482
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2104    2139     529 
   Size:         35    1540      35    3872

  Fragments arranged by size: 

                3872    1540      35      35
 
DraI TTT'AAA

Cuts at:   2593    2700    2786    2593 
   Size:        107      86    5289

  Fragments arranged by size: 

                5289     107      86
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    4489     809 
   Size:        507    3173    1802

  Fragments arranged by size: 

                3173    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2929    3818    5447 
   Size:         54    1425      50     275      18    1099     889    1629

Cuts at:   5447       8 
   Size:         43

  Fragments arranged by size: 

                1629    1425    1099     889     275      54      50      43
                  18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2929    1537 
   Size:        275    1117    4090

  Fragments arranged by size: 

                4090    1117     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2020    2264    3412    1353 
   Size:        667     244    1148    3423

  Fragments arranged by size: 

                3423    1148     667     244
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    4069    4443    4589     387 
   Size:       1064     138    2480     374     146    1280

  Fragments arranged by size: 

                2480    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2245    3545    4142    4929    5197    1239 
   Size:        393     613    1300     597     787     268    1524

  Fragments arranged by size: 

                1524    1300     787     613     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2348    2791    3169    2348 
   Size:        443     378    4661

  Fragments arranged by size: 

                4661     443     378
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2725    2725 
   Size:       5482
 
FspI TGC'GCA

Cuts at:   3988    3988 
   Size:       5482
 
HaeII r_GCGC'y

Cuts at:   1678    4629    1678 
   Size:       2951    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2457    2483    2489    2515    4123 
   Size:         32     135      32     885      26       6      26    1608

Cuts at:   4123    4155    4197    4229    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    1608     885     135      42      32      32      32
                  32      26      26       6
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2826    3072     669 
   Size:        988     281     888     246    3079

  Fragments arranged by size: 

                3079     988     888     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       5482
 
HpaI GTT'AAC

Cuts at:   3072    3072 
   Size:       5482
 
KpnI G_GTAC'C

Cuts at:   2807    2807 
   Size:       5482
 
MfeI C'AATT_G

Cuts at:   2748    3081    2748 
   Size:        333    5149
 
MluI A'CGCG_T

Cuts at:   5358    5358 
   Size:       5482
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4596    4780    1305 
   Size:       3291     184    2007

  Fragments arranged by size: 

                3291    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832      10 
   Size:         54    1425     343    3660

  Fragments arranged by size: 

                3660    1425     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2105    2760    3440    3799    3958     518 
   Size:        648     939     655     680     359     159    2042

  Fragments arranged by size: 

                2042     939     680     655     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2898    3575    4723    4968    1606 
   Size:       1292     677    1148     245    2120

  Fragments arranged by size: 

                2120    1292    1148     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004     513 
   Size:        552     939    3991

  Fragments arranged by size: 

                3991     939     552
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       5482
 
NheI G'CTAG_C

Cuts at:   1052    1052 
   Size:       5482
 
NotI GC'GGCC_GC

Cuts at:   2929    2929 
   Size:       5482
 
NspI r_CATG'y

Cuts at:   1132    1229    2270    2840    4385    1132 
   Size:         97    1041     570    1545    2229

  Fragments arranged by size: 

                2229    1545    1041     570      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       5482
 
PciI A'CATG_T

Cuts at:   4381    4381 
   Size:       5482
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       5482
 
PmeI GTTT'AAAC

Cuts at:   2786    2786 
   Size:       5482
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3551    3583    5064    5096    3551 
   Size:         32    1481      32    3937

  Fragments arranged by size: 

                3937    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       5482
 
PsiI TTA'TAA

Cuts at:   3052    3052 
   Size:       5482
 
PstI C_TGCA'G

Cuts at:    830    2834     830 
   Size:       2004    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2455    2001 
   Size:        454    5028
 
SalI G'TCGA_C

Cuts at:   1936    2824    1936 
   Size:        888    4594
 
SbfI CC_TGCA'GG

Cuts at:   2834    2834 
   Size:       5482
 
ScaI AGT'ACT

Cuts at:   3730    5442    3730 
   Size:       1712    3770
 
SfcI C'TryA_G

Cuts at:    826    1045    2830    3965    4646    4837     826 
   Size:        219    1785    1135     681     191    1471

  Fragments arranged by size: 

                1785    1471    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       5482
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       5482
 
SmaI CCC'GGG

Cuts at:   1872    2809    1872 
   Size:        937    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2360    2672    3586    4487    4749     820 
   Size:        197     926     417     312     914     901     262    1553

  Fragments arranged by size: 

                1553     926     914     901     417     312     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    3273     493 
   Size:       2780    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       5482
 
SphI G_CATG'C

Cuts at:   1132    2840    1132 
   Size:       1708    3774
 
SspI AAT'ATT

Cuts at:      5      52    3406       5 
   Size:         47    3354    2081

  Fragments arranged by size: 

                3354    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       5482
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    2661    2921     513 
   Size:        552     939     657     260    3074

  Fragments arranged by size: 

                3074     939     657     552     260
 
SwaI ATTT'AAAT

Cuts at:   2593    2593 
   Size:       5482
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2428    3509    3526    3679    3864    4309     325 
   Size:       1255     848    1081      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255    1081     848     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2629    3728    5440 
   Size:        274      80      33      51    1443     652    1099    1712

Cuts at:   5440      96 
   Size:        138

  Fragments arranged by size: 

                1712    1443    1099     652     274     138      80      51
                  33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5442    5474    5442 
   Size:         32    5450
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       5482
 
XbaI T'CTAG_A

Cuts at:   2818    2818 
   Size:       5482
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2539    2539 
   Size:       5482
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       5482
 
XmaI C'CCGG_G

Cuts at:   1870    2807    1870 
   Size:        937    4545
 
XmnI GAAnn'nnTTC

Cuts at:   2313    2352    3611    2313 
   Size:         39    1259    4184

  Fragments arranged by size: 

                4184    1259      39
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    3290     276 
   Size:         53      83     186     936    1756    2468

  Fragments arranged by size: 

                2468    1756     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI
     BclI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI
     NdeI     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI
     PpiI    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI
     SfcI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     ApaI     AscI    AvrII     BaeI     BbeI    BbvCI
    BfrBI     BplI   Bpu10I    BsiWI    BsmBI    BstXI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FseI    FspAI     KasI
     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI    SacII
    SanDI     SapI    SexAI     SfiI     SfoI     SrfI
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