################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII KasI KpnI KspI MaeI MaeII MfeI MluI MroI MunI MvnI NaeI NarI NciI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccI 709 GTATAC GT^[AC][GT]AC AlwI 78 GATCC GGATC(4/5) ApaI 345 GGGCCC GGGCC^C Asp700I 1149 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 2 TGGCCA TGG^CCA BanII 345 GGGCCC G[GA]GC[CT]^C BglII 640 AGATCT A^GATCT BmyI 345 GGGCCC G[AGT]GC[ACT]^C BsaBI 1005 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmFI 23 GTCCC GGGAC(10/14) Bsp1286I 345 GGGCCC G[AGT]GC[ACT]^C BspHI 173 TCATGA T^CATGA BsrDI 378 CATTGC GCAATG(2/0) Bst1107I 709 GTATAC GTA^TAC BstAPI 365 GCAGACTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 640 AGATCT [GA]^GATC[CT] BstZ17I 709 GTATAC GTA^TAC Cac8I 109 GCTCGC GC[ACGT]^[ACGT]GC DraIII 987 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 2 TGGCCA [CT]^GGCC[GA] HapII 938 CCGG C^CGG HgaI 966 GACGC GACGC(5/10) HpaII 938 CCGG C^CGG HphI 182 TCACC GGTGA(8/7) MaeIII 396 GTTAC ^GT[ACGT]AC MamI 1005 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 640 AGATCT [GA]^GATC[CT] MluNI 2 TGGCCA TGG^CCA MscI 2 TGGCCA TGG^CCA MspA1I 1107 CAGCGG C[AC]G^C[GT]G MspI 938 CCGG C^CGG NdeI 660 CATATG CA^TATG PpuMI 1127 AGGACCC [GA]G^G[AT]CC[CT] PstI 372 CTGCAG CTGCA^G RcaI 173 TCATGA T^CATGA SfcI 372 CTGCAG C^T[GA][CT]AG TaqI 1075 TCGA T^CGA TfiI 232 GATTC G^A[AT]TC TthHB8I 1075 TCGA T^CGA XhoII 640 AGATCT [GA]^GATC[CT] XmnI 1149 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 977 GCGG CCGC(-3/-1) 1109 GCGG AfaI 738 GTAC GT^AC 1096 GTAC AlwNI 366 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG 950 CAGCAACTG AvaII 656 GGTCC G^G[AT]CC 1128 GGACC BsrI 399 ACTGG ACTGG(1/-1) 1043 CCAGT BssKI 83 CCAGG ^CC[ACGT]GG 794 CCTGG BstNI 83 CCAGG CC^[AT]GG 794 CCTGG DpnII 78 GATC ^GATC 641 GATC EcoRII 83 CCAGG ^CC[AT]GG 794 CCTGG HpaI 745 GTTAAC GTT^AAC 1174 GTTAAC MboI 78 GATC ^GATC 641 GATC MvaI 83 CCAGG CC^[AT]GG 794 CCTGG NdeII 78 GATC ^GATC 641 GATC RsaI 738 GTAC GT^AC 1096 GTAC Sau3AI 78 GATC ^GATC 641 GATC ScrFI 83 CCAGG CC^[ACGT]GG 794 CCTGG SfaNI 404 GATGC GCATC(5/9) 1060 GCATC XcmI 185 CCATGGTAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1043 CCAGTTTTCTCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AluI BbsI BbvI BpuAI BsaJI BsiYI BslI BsmAI BstF5I Cfr13I DdeI DraII DsaI EarI EcoO109I EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI ItaI Ksp632I MboII MnlI MseI MslI MwoI NcoI NlaIII NlaIV PleI Sau96I StyI Tru9I TseI Tsp509I TspRI