(Circular) (Six-base) MAPSORT of: pFN21AE5505.seq Check: 5195 from: 1 to: 5965 pFN21AE5505 5965 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3775 278 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2715 3308 1937 Size: 778 593 4594 Fragments arranged by size: 4594 778 593 Acc65I G'GTAC_C Cuts at: 3286 3286 Size: 5965 AclI AA'CG_TT Cuts at: 1563 4092 4465 1563 Size: 2529 373 3063 Fragments arranged by size: 3063 2529 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2892 4028 5412 5680 1632 Size: 1260 1136 1384 268 1917 Fragments arranged by size: 1917 1384 1260 1136 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5768 AflIII A'CryG_T Cuts at: 1203 4864 5841 1203 Size: 3661 977 1327 Fragments arranged by size: 3661 1327 977 AgeI A'CCGG_T Cuts at: 4796 4796 Size: 5965 AhdI GACnn_n'nnGTC Cuts at: 4694 4694 Size: 5965 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5965 AlwNI CAG_nnn'CTG Cuts at: 2376 2960 3708 5280 2376 Size: 584 748 1572 3061 Fragments arranged by size: 3061 1572 748 584 ApaI G_GGCC'C Cuts at: 2354 2354 Size: 5965 ApaLI G'TGCA_C Cuts at: 4022 5178 4022 Size: 1156 4809 ApoI r'AATT_y Cuts at: 1493 3274 3500 3652 5761 1493 Size: 1781 226 152 2109 1697 Fragments arranged by size: 2109 1781 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4519 160 Size: 872 3487 1606 Fragments arranged by size: 3487 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 AvaI C'yCGr_G Cuts at: 1870 1943 3290 1870 Size: 73 1347 4545 Fragments arranged by size: 4545 1347 73 BamHI G'GATC_C Cuts at: 3295 3662 3295 Size: 367 5598 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3286 4741 618 Size: 325 217 627 1499 1455 1842 Fragments arranged by size: 1842 1499 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2354 3284 1391 Size: 963 930 4072 Fragments arranged by size: 4072 963 930 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2288 2461 2601 3151 928 Size: 971 389 173 140 550 3742 Fragments arranged by size: 3742 971 550 389 173 140 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4156 4190 1903 Size: 34 2219 34 3678 Fragments arranged by size: 3678 2219 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3857 5067 3857 Size: 1210 4755 BclI T'GATC_A Cuts at: 1548 1548 Size: 5965 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4576 136 Size: 107 122 71 631 605 2904 1525 Fragments arranged by size: 2904 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2645 5960 2645 Size: 3315 2650 BlpI GC'TnA_GC Cuts at: 3382 3382 Size: 5965 Bme1580I G_kGCm'C Cuts at: 1790 2354 4026 5182 1790 Size: 564 1672 1156 2573 Fragments arranged by size: 2573 1672 1156 564 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5965 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2414 3696 4654 Size: 416 53 83 399 24 997 1282 958 Cuts at: 4654 442 Size: 1753 Fragments arranged by size: 1753 1282 997 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5965 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4625 1239 Size: 3386 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4090 4955 5253 4090 Size: 865 298 4802 Fragments arranged by size: 4802 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4628 882 Size: 617 3129 2219 Fragments arranged by size: 3129 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3756 493 Size: 711 2552 2702 Fragments arranged by size: 2702 2552 711 BsaBI GATnn'nnATC Cuts at: 3014 5959 3014 Size: 2945 3020 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3772 4154 4789 275 Size: 53 83 186 936 2239 382 635 1451 Fragments arranged by size: 2239 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4398 4796 5070 5217 5628 4398 Size: 398 274 147 411 4735 Fragments arranged by size: 4735 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5740 Fragments arranged by size: 5740 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5965 BseYI C'CCAG_C Cuts at: 2226 5168 5664 2226 Size: 2942 496 2527 Fragments arranged by size: 2942 2527 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5392 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3415 4176 5204 1540 Size: 275 186 1414 761 1028 2301 Fragments arranged by size: 2301 1414 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4026 4111 5182 4026 Size: 85 1071 4809 Fragments arranged by size: 4809 1071 85 BsmI GAATG_Cn' Cuts at: 3476 3569 3476 Size: 93 5872 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2354 3284 4026 4111 5182 1391 Size: 399 564 930 742 85 1071 2174 Fragments arranged by size: 2174 1071 930 742 564 399 85 BspEI T'CCGG_A Cuts at: 5628 5628 Size: 5965 BspHI T'CATG_A Cuts at: 2178 3854 5584 2178 Size: 1676 1730 2559 Fragments arranged by size: 2559 1730 1676 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3320 844 Size: 2476 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3852 1394 Size: 429 2029 3507 Fragments arranged by size: 3507 2029 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2382 4460 4634 65 Size: 1183 1134 2078 174 1396 Fragments arranged by size: 2078 1396 1183 1134 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4609 4796 1700 Size: 110 2799 187 2869 Fragments arranged by size: 2869 2799 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5965 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5965 BssSI C'ACGA_G Cuts at: 4025 5037 4025 Size: 1012 4953 BstAPI GCAn_nnn'nTGC Cuts at: 794 2376 794 Size: 1582 4383 BstBI TT'CG_AA Cuts at: 4769 4769 Size: 5965 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5965 BstYI r'GATC_y Cuts at: 1972 2645 3295 3662 4047 4064 5516 5602 5614 Size: 673 650 367 385 17 1452 86 12 Cuts at: 5614 5960 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 673 650 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2716 2716 Size: 5965 BtgI C'CryG_G Cuts at: 513 1065 2004 2190 2199 2549 513 Size: 552 939 186 9 350 3929 Fragments arranged by size: 3929 939 552 350 186 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5226 Fragments arranged by size: 5226 683 56 BtsI GCAGTG_nn' Cuts at: 3481 4275 4295 3481 Size: 794 20 5151 Fragments arranged by size: 5151 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5965 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5930 DraI TTT'AAA Cuts at: 3269 3269 Size: 5965 DraIII CAC_nnn'GTG Cuts at: 2997 2997 Size: 5965 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4972 809 Size: 507 3656 1802 Fragments arranged by size: 3656 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 3412 4301 Size: 54 1425 50 275 18 177 1405 889 Cuts at: 4301 5930 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1405 889 275 177 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3412 1537 Size: 275 1600 4090 Fragments arranged by size: 4090 1600 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2628 2911 3203 3895 1353 Size: 1275 283 292 692 3423 Fragments arranged by size: 3423 1275 692 292 283 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4552 4926 5072 387 Size: 1064 138 2963 374 146 1280 Fragments arranged by size: 2963 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2892 4028 4625 5412 5680 1239 Size: 393 1260 1136 597 787 268 1524 Fragments arranged by size: 1524 1260 1136 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2350 2351 3133 2350 Size: 1 782 5182 Fragments arranged by size: 5182 782 1 EcoRI G'AATT_C Cuts at: 3274 3652 3274 Size: 378 5587 FspI TGC'GCA Cuts at: 4471 4471 Size: 5965 HaeII r_GCGC'y Cuts at: 1678 5112 1678 Size: 3434 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4606 4638 4680 4712 1373 Size: 32 135 32 3034 32 42 32 2626 Fragments arranged by size: 3034 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 2260 2752 3181 3309 3555 Size: 988 281 268 54 492 429 128 246 Cuts at: 3555 669 Size: 3079 Fragments arranged by size: 3079 988 492 429 281 268 246 128 54 HindIII A'AGCT_T Cuts at: 748 748 Size: 5965 HpaI GTT'AAC Cuts at: 2752 3181 3555 2752 Size: 429 374 5162 Fragments arranged by size: 5162 429 374 KpnI G_GTAC'C Cuts at: 3290 3290 Size: 5965 MfeI C'AATT_G Cuts at: 3564 3564 Size: 5965 MluI A'CGCG_T Cuts at: 5841 5841 Size: 5965 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5079 5263 1305 Size: 3774 184 2007 Fragments arranged by size: 3774 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2009 10 Size: 54 1425 343 177 3966 Fragments arranged by size: 3966 1425 343 177 54 MslI CAynn'nnrTG Cuts at: 518 1166 2189 2810 2867 3923 4282 4441 518 Size: 648 1023 621 57 1056 359 159 2042 Fragments arranged by size: 2042 1056 1023 648 621 359 159 57 MspA1I CmG'CkG Cuts at: 1606 3114 3381 4058 5206 5451 1606 Size: 1508 267 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 267 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2190 2199 2549 513 Size: 552 939 186 9 350 3929 Fragments arranged by size: 3929 939 552 350 186 9 NdeI CA'TA_TG Cuts at: 387 2666 387 Size: 2279 3686 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5965 NotI GC'GGCC_GC Cuts at: 3412 3412 Size: 5965 NspI r_CATG'y Cuts at: 1132 1229 3323 4868 1132 Size: 97 2094 1545 2229 Fragments arranged by size: 2229 2094 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5965 PciI A'CATG_T Cuts at: 4864 4864 Size: 5965 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5965 PmeI GTTT'AAAC Cuts at: 3269 3269 Size: 5965 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4034 4066 5547 5579 4034 Size: 32 1481 32 4420 Fragments arranged by size: 4420 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3133 3133 Size: 5965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5965 PsiI TTA'TAA Cuts at: 3535 3535 Size: 5965 PspOMI G'GGCC_C Cuts at: 2350 2350 Size: 5965 PstI C_TGCA'G Cuts at: 830 2381 3317 830 Size: 1551 936 3478 Fragments arranged by size: 3478 1551 936 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5965 SalI G'TCGA_C Cuts at: 1936 3307 1936 Size: 1371 4594 SbfI CC_TGCA'GG Cuts at: 3317 3317 Size: 5965 ScaI AGT'ACT Cuts at: 4213 5925 4213 Size: 1712 4253 SfcI C'TryA_G Cuts at: 826 1045 2377 3313 4448 5129 5320 826 Size: 219 1332 936 1135 681 191 1471 Fragments arranged by size: 1471 1332 1135 936 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5965 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5965 SmaI CCC'GGG Cuts at: 1872 3292 1872 Size: 1420 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4069 4970 5232 820 Size: 197 926 2126 901 262 1553 Fragments arranged by size: 2126 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3756 493 Size: 3263 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5965 SphI G_CATG'C Cuts at: 1132 3323 1132 Size: 2191 3774 SspI AAT'ATT Cuts at: 5 52 3889 5 Size: 47 3837 2081 Fragments arranged by size: 3837 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5965 StyI C'CwwG_G Cuts at: 513 1065 2004 2190 2199 2452 2549 3038 3404 Size: 552 939 186 9 253 97 489 366 Cuts at: 3404 513 Size: 3074 Fragments arranged by size: 3074 939 552 489 366 253 186 97 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2445 3135 3992 4009 4162 4347 4792 Size: 1255 865 690 857 17 153 185 445 Cuts at: 4792 325 Size: 1498 Fragments arranged by size: 1498 1255 865 857 690 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4211 5923 96 Size: 274 80 33 51 1443 2234 1712 138 Fragments arranged by size: 2234 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5925 5957 5925 Size: 32 5933 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5965 XbaI T'CTAG_A Cuts at: 3301 3301 Size: 5965 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2197 3055 2197 Size: 858 5107 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5965 XmaI C'CCGG_G Cuts at: 1870 3290 1870 Size: 1420 4545 XmnI GAAnn'nnTTC Cuts at: 3158 4094 3158 Size: 936 5029 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3773 276 Size: 53 83 186 936 2239 2468 Fragments arranged by size: 2468 2239 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI