Restriction map for
pFN21AE1476
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# NO CUT ENZYMES ( 99 enzymes) #
##################################
AatII AccI AclI AcyI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI AspEI
AviII AvrII BbeI BbrPI BfrI
BglII BlnI BsaAI BsaHI BsaI
BsiEI BsiWI BsmBI Bsp1407I BspHI
BspLU11I BsrGI BssHII Bst1107I BstEII
BstPI BstZ17I CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoT22I FseI FspI
Hin1I HpaI KasI KspI MfeI
MluI MseI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Van91I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 520 GGTACC G^GTACC
AccIII 1109 TCCGGA T^CCGGA
AcsI 1593 GAATTC [GA]^AATT[CT]
AocI 1825 CCTGAGG CC^T[ACGT]AGG
ApoI 1593 GAATTC [GA]^AATT[CT]
Asp700I 1846 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 520 GGTACC G^GTACC
AspI 622 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 1149 GGATCC G^GATCC
BanI 520 GGTACC G^G[CT][GA]CC
BbvCI 1861 GCTGAGG CCTCAGC(-5/-2)
BciVI 1106 GTATCC GTATCC(6/5)
BclI 596 TGATCA T^GATCA
BglI 398 GCCCACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 651 CTCCAG CTGGAG(16/14)
BsaBI 1294 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1109 TCCGGA T^CCGGA
BsgI 1101 GTGCAG GTGCAG(16/14)
BsmI 388 GAATGC GAATGC(1/-1)
BspDI 1246 ATCGAT AT^CGAT
BspEI 1109 TCCGGA T^CCGGA
BssSI 1366 CACGAG CACGAG(-5/-1)
BstAPI 263 GCAGCAGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1885 TTCGAA TT^CGAA
Bsu36I 1825 CCTGAGG CC^T[ACGT]AGG
ClaI 1246 ATCGAT AT^CGAT
Eco81I 1825 CCTGAGG CC^T[ACGT]AGG
EcoNI 284 CCTTTACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1593 GAATTC G^AATTC
FbaI 596 TGATCA T^GATCA
HaeII 1438 AGCGCC [GA]GCGC^[CT]
HgaI 257 GCGTC GACGC(5/10)
HindIII 1258 AAGCTT A^AGCTT
KpnI 520 GGTACC GGTAC^C
MamI 1294 GATATCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1109 TCCGGA T^CCGGA
NspV 1885 TTCGAA TT^CGAA
PaeR7I 1119 CTCGAG C^TCGAG
PflFI 622 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuII 1507 CAGCTG CAG^CTG
SexAI 587 ACCTGGT A^CC[AT]GGT
SfuI 1885 TTCGAA TT^CGAA
StuI 137 AGGCCT AGG^CCT
Tth111I 622 GACAACGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1154 CCAGTGCCATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1119 CTCGAG C^TCGAG
XmnI 1846 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AspHI 512 GTGCTC G[AT]GC[AT]^C
700 GAGCAC
BalI 343 TGGCCA TGG^CCA
1236 TGGCCA
BbsI 856 GAAGAC GAAGAC(2/6)
1495 GAAGAC
BpuAI 856 GAAGAC GAAGAC(2/6)
1495 GAAGAC
BsaWI 1109 TCCGGA [AT]^CCGG[AT]
1454 ACCGGA
BseRI 1698 CTCCTC GAGGAG(10/8)
1828 GAGGAG
BsiHKAI 512 GTGCTC G[AT]GC[AT]^C
700 GAGCAC
BspMI 1958 ACCTGC ACCTGC(4/8)
1761 GCAGGT
BsrBI 1598 CCGCTC CCGCTC(-3/-3)
1369 GAGCGG
BsrDI 1232 GCAATG GCAATG(2/0)
1313 GCAATG
BsrFI 181 ACCGGC [GA]^CCGG[CT]
1877 ACCGGC
BstXI 334 CCATGCCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1502 CCACCCAGCTGG
BstYI 1149 GGATCC [GA]^GATC[CT]
1220 GGATCT
Cfr10I 181 ACCGGC [GA]^CCGG[CT]
1877 ACCGGC
EarI 771 CTCTTC CTCTTC(1/4)
1846 GAAGAG
EcoRV 1249 GATATC GAT^ATC
1294 GATATC
HincII 169 GTTGAC GT[CT]^[GA]AC
892 GTCAAC
HindII 169 GTTGAC GT[CT]^[GA]AC
892 GTCAAC
Ksp632I 771 CTCTTC CTCTTC(1/4)
1846 GAAGAG
MaeII 626 ACGT A^CGT
860 ACGT
MflI 1149 GGATCC [GA]^GATC[CT]
1220 GGATCT
MluNI 343 TGGCCA TGG^CCA
1236 TGGCCA
MscI 343 TGGCCA TGG^CCA
1236 TGGCCA
MslI 295 CATGAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
335 CATGCCCATG
NspI 407 GCATGT [GA]CATG^[CT]
1805 ACATGC
PpuMI 4 GGGACCT [GA]G^G[AT]CC[CT]
280 GGGACCT
PshAI 202 GACAAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
322 GACTGTAGTC
SfaNI 47 GCATC GCATC(5/9)
806 GCATC
SmlI 1119 CTCGAG C^T[CT][GA]AG
1588 CTTGAG
XhoII 1149 GGATCC [GA]^GATC[CT]
1220 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AvaI AvaII BanII
BbvI BcgI BcnI BfaI BlpI
BmyI Bpu1102I BsaJI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV PleI PstI RsaI
Sau3AI Sau96I ScrFI SfcI StyI
TaqI TfiI TseI Tsp45I Tsp509I
TspRI TthHB8I