(Linear) (Six-base) MAPSORT of: KIEE1476.seq Check: 5278 from: 1 to: 1965 KIEE1476.orf With 182 enzymes: SgfI * July 15, 2010 18:39 .. Acc65I G'GTAC_C Cuts at: 0 520 1965 Size: 520 1445 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 868 949 1201 1276 1965 Size: 868 81 252 75 689 Fragments arranged by size: 868 689 252 81 75 AlwNI CAG_nnn'CTG Cuts at: 0 487 588 826 1965 Size: 487 101 238 1139 Fragments arranged by size: 1139 487 238 101 ApaI G_GGCC'C Cuts at: 0 991 1142 1411 1965 Size: 991 151 269 554 Fragments arranged by size: 991 554 269 151 ApoI r'AATT_y Cuts at: 0 1593 1965 Size: 1593 372 AvaI C'yCGr_G Cuts at: 0 515 1119 1411 1557 1965 Size: 515 604 292 146 408 Fragments arranged by size: 604 515 408 292 146 BamHI G'GATC_C Cuts at: 0 1149 1965 Size: 1149 816 BanI G'GyrC_C Cuts at: 0 520 1965 Size: 520 1445 BanII G_rGCy'C Cuts at: 0 85 991 1142 1411 1543 1564 1667 1965 Size: 85 906 151 269 132 21 103 298 Fragments arranged by size: 906 298 269 151 132 103 85 21 BbsI GAAGACnn'nnnn_ Cuts at: 0 863 1502 1965 Size: 863 639 463 Fragments arranged by size: 863 639 463 BbvCI CC'TCA_GC Cuts at: 0 1862 1965 Size: 1862 103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 12 30 46 64 496 530 1965 Size: 12 18 16 18 432 34 1435 Fragments arranged by size: 1435 432 34 18 18 16 12 BciVI GTATCCnnnnn_n' Cuts at: 0 1117 1965 Size: 1117 848 BclI T'GATC_A Cuts at: 0 596 1965 Size: 596 1369 BglI GCCn_nnn'nGGC Cuts at: 0 404 1965 Size: 404 1561 BlpI GC'TnA_GC Cuts at: 0 122 840 1552 1708 1965 Size: 122 718 712 156 257 Fragments arranged by size: 718 712 257 156 122 Bme1580I G_kGCm'C Cuts at: 0 435 991 1142 1411 1965 Size: 435 556 151 269 554 Fragments arranged by size: 556 554 435 269 151 BmgBI CAC'GTC Cuts at: 0 861 1965 Size: 861 1104 BmrI ACTGGGnnnn_n' Cuts at: 0 965 1148 1965 Size: 965 183 817 Fragments arranged by size: 965 817 183 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 1965 Size: 636 1329 Bpu10I CC'TnA_GC Cuts at: 0 106 698 1862 1965 Size: 106 592 1164 103 Fragments arranged by size: 1164 592 106 103 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1609 1965 Size: 1609 356 BsaBI GATnn'nnATC Cuts at: 0 1298 1965 Size: 1298 667 BsaWI w'CCGG_w Cuts at: 0 1109 1454 1965 Size: 1109 345 511 Fragments arranged by size: 1109 511 345 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1514 1544 1965 Size: 1514 30 421 Fragments arranged by size: 1514 421 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1689 1843 1965 Size: 1689 154 122 Fragments arranged by size: 1689 154 122 BseYI C'CCAG_C Cuts at: 0 1427 1505 1543 1570 1965 Size: 1427 78 38 27 395 Fragments arranged by size: 1427 395 78 38 27 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1122 1965 Size: 1122 843 BsiHKAI G_wGCw'C Cuts at: 0 516 704 1965 Size: 516 188 1261 Fragments arranged by size: 1261 516 188 BsmI GAATG_Cn' Cuts at: 0 394 1965 Size: 394 1571 Bsp1286I G_dGCh'C Cuts at: 0 85 435 516 704 991 1142 1411 1543 Size: 85 350 81 188 287 151 269 132 Cuts at: 1543 1564 1667 1965 Size: 21 103 298 Fragments arranged by size: 350 298 287 269 188 151 132 103 85 81 21 BspEI T'CCGG_A Cuts at: 0 1109 1965 Size: 1109 856 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1752 1965 Size: 1752 213 BsrBI CCG'CTC Cuts at: 0 1371 1600 1965 Size: 1371 229 365 Fragments arranged by size: 1371 365 229 BsrDI GCAATG_nn' Cuts at: 0 1239 1320 1965 Size: 1239 81 645 Fragments arranged by size: 1239 645 81 BsrFI r'CCGG_y Cuts at: 0 181 1877 1965 Size: 181 1696 88 Fragments arranged by size: 1696 181 88 BssSI C'ACGA_G Cuts at: 0 1366 1965 Size: 1366 599 BstAPI GCAn_nnn'nTGC Cuts at: 0 269 1965 Size: 269 1696 BstBI TT'CG_AA Cuts at: 0 1886 1965 Size: 1886 79 BstXI CCAn_nnnn'nTGG Cuts at: 0 341 1509 1965 Size: 341 1168 456 Fragments arranged by size: 1168 456 341 BstYI r'GATC_y Cuts at: 0 1149 1220 1965 Size: 1149 71 745 Fragments arranged by size: 1149 745 71 Bsu36I CC'TnA_GG Cuts at: 0 1826 1965 Size: 1826 139 BtgI C'CryG_G Cuts at: 0 340 944 1239 1965 Size: 340 604 295 726 Fragments arranged by size: 726 604 340 295 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 33 1965 Size: 33 1932 BtsI GCAGTG_nn' Cuts at: 0 514 1965 Size: 514 1451 ClaI AT'CG_AT Cuts at: 0 1247 1965 Size: 1247 718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 612 647 1965 Size: 612 35 1318 Fragments arranged by size: 1318 612 35 EaeI y'GGCC_r Cuts at: 0 183 343 1236 1965 Size: 183 160 893 729 Fragments arranged by size: 893 729 183 160 EarI CTCTTCn'nnn_ Cuts at: 0 777 1841 1965 Size: 777 1064 124 Fragments arranged by size: 1064 777 124 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 636 868 949 1201 1276 1965 Size: 636 232 81 252 75 689 Fragments arranged by size: 689 636 252 232 81 75 EcoNI CCTnn'n_nnAGG Cuts at: 0 288 1965 Size: 288 1677 EcoO109I rG'GnC_Cy Cuts at: 0 5 281 907 987 988 1138 1139 1407 Size: 5 276 626 80 1 150 1 268 Cuts at: 1407 1408 1965 Size: 1 557 Fragments arranged by size: 626 557 276 268 150 80 5 1 1 1 EcoRI G'AATT_C Cuts at: 0 1593 1965 Size: 1593 372 EcoRV GAT'ATC Cuts at: 0 1251 1296 1965 Size: 1251 45 669 Fragments arranged by size: 1251 669 45 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1662 1965 Size: 1662 303 HaeII r_GCGC'y Cuts at: 0 1442 1965 Size: 1442 523 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 987 1019 1672 1704 1965 Size: 987 32 653 32 261 Fragments arranged by size: 987 653 261 32 32 HincII GTy'rAC Cuts at: 0 171 894 1965 Size: 171 723 1071 Fragments arranged by size: 1071 723 171 HindIII A'AGCT_T Cuts at: 0 1258 1965 Size: 1258 707 KpnI G_GTAC'C Cuts at: 0 524 1965 Size: 524 1441 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 791 1222 1640 1965 Size: 791 431 418 325 Fragments arranged by size: 791 431 418 325 MscI TGG'CCA Cuts at: 0 345 1238 1965 Size: 345 893 727 Fragments arranged by size: 893 727 345 MslI CAynn'nnrTG Cuts at: 0 299 339 1965 Size: 299 40 1626 Fragments arranged by size: 1626 299 40 MspA1I CmG'CkG Cuts at: 0 1509 1676 1694 1965 Size: 1509 167 18 271 Fragments arranged by size: 1509 271 167 18 NcoI C'CATG_G Cuts at: 0 340 944 1239 1965 Size: 340 604 295 726 Fragments arranged by size: 726 604 340 295 NspI r_CATG'y Cuts at: 0 411 1809 1965 Size: 411 1398 156 Fragments arranged by size: 1398 411 156 PfoI T'CCnGG_A Cuts at: 0 809 1928 1965 Size: 809 1119 37 Fragments arranged by size: 1119 809 37 PpuMI rG'GwC_Cy Cuts at: 0 5 281 1965 Size: 5 276 1684 Fragments arranged by size: 1684 276 5 PshAI GACnn'nnGTC Cuts at: 0 206 326 1965 Size: 206 120 1639 Fragments arranged by size: 1639 206 120 PspOMI G'GGCC_C Cuts at: 0 987 1138 1407 1965 Size: 987 151 269 558 Fragments arranged by size: 987 558 269 151 PspXI vC'TCGA_Gb Cuts at: 0 1119 1965 Size: 1119 846 PstI C_TGCA'G Cuts at: 0 43 265 1391 1965 Size: 43 222 1126 574 Fragments arranged by size: 1126 574 222 43 PvuII CAG'CTG Cuts at: 0 1509 1965 Size: 1509 456 SexAI A'CCwGG_T Cuts at: 0 587 1965 Size: 587 1378 SfcI C'TryA_G Cuts at: 0 39 261 324 1387 1965 Size: 39 222 63 1063 578 Fragments arranged by size: 1063 578 222 63 39 SmlI C'TyrA_G Cuts at: 0 1119 1588 1965 Size: 1119 469 377 Fragments arranged by size: 1119 469 377 StuI AGG'CCT Cuts at: 0 139 1965 Size: 139 1826 StyI C'CwwG_G Cuts at: 0 340 611 944 1239 1965 Size: 340 271 333 295 726 Fragments arranged by size: 726 340 333 295 271 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 398 1519 1965 Size: 398 1121 446 Fragments arranged by size: 1121 446 398 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 484 516 723 755 1965 Size: 484 32 207 32 1210 Fragments arranged by size: 1210 484 207 32 32 Tth111I GACn'n_nGTC Cuts at: 0 625 1965 Size: 625 1340 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1161 1965 Size: 1161 804 XhoI C'TCGA_G Cuts at: 0 1119 1965 Size: 1119 846 XmnI GAAnn'nnTTC Cuts at: 0 1850 1965 Size: 1850 115 Enzymes that do cut: Acc65I AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI EarI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI MspA1I NcoI NspI PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SexAI SfcI SmlI StuI StyI TaqII TstI Tth111I XcmI XhoI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbeI BfrBI BglII BmtI BplI BsaI BsaAI BsaHI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BstEII BstZ17I DraI DraIII DrdI EagI EciI EcoICRI FseI FspI FspAI HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PflMI PmeI PmlI PpiI PsiI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TatI XbaI XmaI ZraI