################################## # NO CUT ENZYMES ( 153 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspLU11I BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HgaI Hin1I HincII HindII HinfI HpaI KasI KpnI KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AlwNI 124 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 607 CCTCAGG CC^T[ACGT]AGG Asp700I 483 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 612 GGTGCC G^G[CT][GA]CC BbsI 586 GAAGAC GAAGAC(2/6) BglI 275 GCCGCAACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 586 GAAGAC GAAGAC(2/6) BsaAI 256 TACGTG [CT]AC^GT[GA] BsiEI 334 CGGCCG CG[GA][CT]^CG Bsp1407I 263 TGTACA T^GTACA BspHI 695 TCATGA T^CATGA BsrGI 263 TGTACA T^GTACA BstAPI 252 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstF5I 479 GGATG GGATG(2/0) Bsu36I 607 CCTCAGG CC^T[ACGT]AGG DpnII 573 GATC ^GATC EagI 334 CGGCCG C^GGCCG EarI 627 GAAGAG CTCTTC(1/4) EclXI 334 CGGCCG C^GGCCG Eco52I 334 CGGCCG C^GGCCG Eco81I 607 CCTCAGG CC^T[ACGT]AGG FokI 479 GGATG GGATG(9/13) HindIII 619 AAGCTT A^AGCTT Ksp632I 627 GAAGAG CTCTTC(1/4) MboI 573 GATC ^GATC NaeI 418 GCCGGC GCC^GGC NcoI 554 CCATGG C^CATGG NdeII 573 GATC ^GATC NgoMIV 418 GCCGGC G^CCGGC NspI 96 GCATGC [GA]CATG^[CT] RcaI 695 TCATGA T^CATGA SapI 627 GAAGAGC GCTCTTC(1/4) Sau3AI 573 GATC ^GATC ScaI 428 AGTACT AGT^ACT SfcI 384 CTACAG C^T[GA][CT]AG SmaI 217 CCCGGG CCC^GGG SmlI 622 CTTGAG C^T[CT][GA]AG SphI 96 GCATGC GCATG^C SspBI 263 TGTACA T^GTACA TspRI 463 CACTG CA[GC]TG[ACGT][ACGT]^ XmaCI 217 CCCGGG C^CCGGG XmaI 217 CCCGGG C^CCGGG XmnI 483 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### Aor51HI 317 AGCGCT AGC^GCT 380 AGCGCT AvaI 204 CTCGGG C^[CT]CG[GA]G 217 CCCGGG BsgI 460 CTGCAC GTGCAG(16/14) 151 GTGCAG BsoBI 204 CTCGGG C^[CT]CG[GA]G 217 CCCGGG BssSI 424 CACGAG CACGAG(-5/-1) 34 CTCGTG BstNI 306 CCAGG CC^[AT]GG 669 CCTGG DdeI 475 CTGAG C^T[ACGT]AG 608 CTCAG DsaI 554 CCATGG C^C[GA][CT]GG 674 CCGTGG Eco47III 317 AGCGCT AGC^GCT 380 AGCGCT EcoRII 306 CCAGG ^CC[AT]GG 669 CCTGG EcoT14I 66 CCAAGG C^C[AT][AT]GG 554 CCATGG MvaI 306 CCAGG CC^[AT]GG 669 CCTGG SfaNI 632 GCATC GCATC(5/9) 680 GCATC StyI 66 CCAAGG C^C[AT][AT]GG 554 CCATGG ######################################### # 3 OR MORE CUT ENZYMES ( 48 enzymes) # ######################################### AciI AfaI AluI ApaI AvaII BanII BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI Cac8I CfoI Cfr10I Cfr13I DraII EaeI EcoO109I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MboII MnlI MspA1I MspI MwoI NciI NlaIII NlaIV RsaI Sau96I ScrFI TseI