################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AgeI AhdI AlwI AlwNI AocI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbrPI BbvCI BciVI BglI BglII BlnI BsaAI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoT22I FseI FspI HgaI HpaI KpnI Ksp632I KspI MfeI MflI MluI MroI MspA1I MunI MvnI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacII SalI SapI SbfI SexAI SfiI SgrAI SnaBI Sse8387I SspBI SspI SwaI XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AcyI 30 GGCGCC G[GA]^CG[CT]C AflII 1216 CTTAAG C^TTAAG AflIII 1121 ACACGT A^C[GA][CT]GT Alw44I 1577 GTGCAC G^TGCAC ApaI 1253 GGGCCC GGGCC^C ApaLI 1577 GTGCAC G^TGCAC AspI 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 3 GGTCC G^G[AT]CC BalI 248 TGGCCA TGG^CCA BbeI 30 GGCGCC GGCGC^C BbsI 1758 GTCTTC GAAGAC(2/6) BcgI 1677 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 617 TGATCA T^GATCA BfaI 1327 CTAG C^TAG BfrI 1216 CTTAAG C^TTAAG BlpI 453 GCTCAGC GC^T[ACGT]AGC BpmI 356 CTGGAG CTGGAG(16/14) Bpu1102I 453 GCTCAGC GC^T[ACGT]AGC BpuAI 1758 GTCTTC GAAGAC(2/6) BsaBI 961 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 30 GGCGCC G[GA]^CG[CT]C BseRI 715 GAGGAG GAGGAG(10/8) BsmAI 1663 GAGAC GTCTC(1/5) BspHI 1410 TCATGA T^CATGA BspMI 1701 ACCTGC ACCTGC(4/8) BsrBI 115 GAGCGG CCGCTC(-3/-3) BsrDI 173 CATTGC GCAATG(2/0) BstAPI 84 GCAACTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 518 TTCGAA TT^CGAA BstXI 655 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 453 GCTCAGC GC^T[ACGT]AGC DsaI 1364 CCGTGG C^C[GA][CT]GG EcoNI 246 CCTGGCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 617 TGATCA T^GATCA Hin1I 30 GGCGCC G[GA]^CG[CT]C HphI 1392 GGTGA GGTGA(8/7) KasI 30 GGCGCC G^GCGCC MaeI 1327 CTAG C^TAG MamI 961 GATAAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 248 TGGCCA TGG^CCA MscI 248 TGGCCA TGG^CCA NarI 30 GGCGCC GG^CGCC NspV 518 TTCGAA TT^CGAA PaeR7I 473 CTCGAG C^TCGAG PflFI 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 225 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 1410 TCATGA T^CATGA ScaI 1535 AGTACT AGT^ACT SfoI 30 GGCGCC GGC^GCC SfuI 518 TTCGAA TT^CGAA SpeI 1326 ACTAGT A^CTAGT SphI 1283 GCATGC GCATG^C Tth111I 1422 GACTGAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 225 CCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 473 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AfaI 1104 GTAC GT^AC 1536 GTAC CfoI 31 GCGC GCG^C 430 GCGC DpnII 280 GATC ^GATC 618 GATC EaeI 182 TGGCCG [CT]^GGCC[GA] 248 TGGCCA EcoRV 347 GATATC GAT^ATC 1468 GATATC EcoT14I 251 CCAAGG C^C[AT][AT]GG 1656 CCAAGG HaeII 30 GGCGCC [GA]GCGC^[CT] 429 GGCGCT HhaI 31 GCGC GCG^C 430 GCGC HinP1I 31 GCGC G^CGC 430 GCGC HincII 1162 GTCAAC GT[CT]^[GA]AC 1419 GTTGAC HindII 1162 GTCAAC GT[CT]^[GA]AC 1419 GTTGAC HindIII 1405 AAGCTT A^AGCTT 1565 AAGCTT MboI 280 GATC ^GATC 618 GATC NdeII 280 GATC ^GATC 618 GATC NspI 1149 ACATGC [GA]CATG^[CT] 1283 GCATGC PleI 1665 GACTC GAGTC(4/5) 1426 GAGTC RsaI 1104 GTAC GT^AC 1536 GTAC SacI 451 GAGCTC GAGCT^C 765 GAGCTC Sau3AI 280 GATC ^GATC 618 GATC SfcI 1176 CTACAG C^T[GA][CT]AG 1642 CTACAG SmaI 110 CCCGGG CCC^GGG 1607 CCCGGG SmlI 473 CTCGAG C^T[CT][GA]AG 1216 CTTAAG StuI 254 AGGCCT AGG^CCT 1622 AGGCCT StyI 251 CCAAGG C^C[AT][AT]GG 1656 CCAAGG TfiI 738 GAATC G^A[AT]TC 1276 GATTC Tsp45I 16 GTGAC ^GT[GC]AC 1765 GTCAC XmaCI 110 CCCGGG C^CCGGG 1607 CCCGGG XmaI 110 CCCGGG C^CCGGG 1607 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AciI AluI AspHI AvaI BanI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII EcoO109I EcoRII FauI Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI MaeII MaeIII MboII MnlI MseI MslI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfaNI TaqI Tru9I TseI Tsp509I TspRI TthHB8I