Restriction map for
pFN21AB5995
##################################
# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AclI AcyI AfaI
AflII AgeI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BbeI
BbrPI BbsI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsgI BsiEI
BsiWI BsmBI Bsp1407I BspDI BsrBI
BsrFI BsrGI BssHII BstBI BstEII
BstPI CelII Cfr10I ClaI CpoI
DrdI EagI EclXI Eco47III Eco52I
EcoO65I EcoRV FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 238 CGCG CG^CG
AccIII 549 TCCGGA T^CCGGA
AflIII 479 ACATGT A^C[GA][CT]GT
AhdI 351 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 351 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1080 TGGCCA TGG^CCA
BanI 319 GGTGCC G^G[CT][GA]CC
BanII 1135 GAGCCC G[GA]GC[CT]^C
BbvCI 613 CCTCAGC CCTCAGC(-5/-2)
BsaWI 549 TCCGGA [AT]^CCGG[AT]
BseAI 549 TCCGGA T^CCGGA
BspEI 549 TCCGGA T^CCGGA
BspHI 1602 TCATGA T^CATGA
BspLU11I 479 ACATGT A^CATGT
BspMI 1626 GCAGGT ACCTGC(4/8)
BstAPI 208 GCACTTAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 238 CGCG CG^CG
DraI 1695 TTTAAA TTT^AAA
DraIII 209 CACTTAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1080 TGGCCA [CT]^GGCC[GA]
Eam1105I 351 GACCATCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 241 GAATTC G^AATTC
EcoT22I 709 ATGCAT ATGCA^T
HindIII 740 AAGCTT A^AGCTT
HphI 337 TCACC GGTGA(8/7)
MaeII 45 ACGT A^CGT
MluNI 1080 TGGCCA TGG^CCA
MroI 549 TCCGGA T^CCGGA
MscI 1080 TGGCCA TGG^CCA
MslI 712 CATGTCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 238 CGCG CG^CG
NsiI 709 ATGCAT ATGCA^T
PaeR7I 953 CTCGAG C^TCGAG
PshAI 863 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1643 CTGCAG CTGCA^G
PvuII 782 CAGCTG CAG^CTG
RcaI 1602 TCATGA T^CATGA
SfcI 1643 CTGCAG C^T[GA][CT]AG
SfiI 681 GGCCCCTCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1573 CCCGGG CCC^GGG
SmlI 953 CTCGAG C^T[CT][GA]AG
TfiI 672 GAATC G^A[AT]TC
XhoI 953 CTCGAG C^TCGAG
XmaCI 1573 CCCGGG C^CCGGG
XmaI 1573 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 142 GTATAC GT^[AC][GT]AC
1538 GTATAC
AcsI 241 GAATTC [GA]^AATT[CT]
566 AAATTC
ApoI 241 GAATTC [GA]^AATT[CT]
566 AAATTC
AspHI 254 GAGCAC G[AT]GC[AT]^C
1724 GAGCAC
BciVI 1395 GGATAC GTATCC(6/5)
1685 GTATCC
BcnI 1573 CCCGG CC^[GC]GG
1574 CCGGG
BsaI 20 GAGACC GGTCTC(1/5)
349 GAGACC
BseRI 728 CTCCTC GAGGAG(10/8)
824 GAGGAG
BsiHKAI 254 GAGCAC G[AT]GC[AT]^C
1724 GAGCAC
BssSI 1235 CACGAG CACGAG(-5/-1)
1325 CTCGTG
Bst1107I 142 GTATAC GTA^TAC
1538 GTATAC
BstZ17I 142 GTATAC GTA^TAC
1538 GTATAC
CfoI 237 GCGC GCG^C
722 GCGC
EcoNI 637 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
820 CCTGGAGGAGG
FauI 4 GCGGG CCCGC(4/6)
921 GCGGG
HapII 550 CCGG C^CGG
1574 CCGG
HhaI 237 GCGC GCG^C
722 GCGC
HinP1I 237 GCGC G^CGC
722 GCGC
HpaII 550 CCGG C^CGG
1574 CCGG
MseI 1463 TTAA T^TAA
1696 TTAA
MspI 550 CCGG C^CGG
1574 CCGG
NciI 1573 CCCGG CC^[GC]GG
1574 CCGGG
NspI 479 ACATGT [GA]CATG^[CT]
711 GCATGT
PleI 334 GAGTC GAGTC(4/5)
376 GAGTC
TaqI 261 TCGA T^CGA
954 TCGA
Tru9I 1463 TTAA T^TAA
1696 TTAA
TthHB8I 261 TCGA T^CGA
954 TCGA
XcmI 693 CCAGTCTTTGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1139 CCAGACCTCTAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AciI AluI AlwI AlwNI AocI
AvaI AvaII BamHI BbvI BfaI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
Cfr13I DdeI DpnII DraII DsaI
EarI Eco81I EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeIII HinfI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MspA1I MvaI MwoI
NcoI NdeII NlaIII NlaIV PflMI
PpuMI SapI Sau3AI Sau96I ScrFI
SfaNI StyI TseI Tsp45I Tsp509I
TspRI Van91I XhoII