(Circular) (Six-base) MAPSORT of: pFN21AB5995.seq Check: 4847 from: 1 to: 6436 pFN21AB5995 6436 bp With 182 enzymes: SgfI * April 15, 2010 09:30 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4246 278 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2148 3544 3779 1937 Size: 211 1396 235 4594 Fragments arranged by size: 4594 1396 235 211 Acc65I G'GTAC_C Cuts at: 3757 3757 Size: 6436 AclI AA'CG_TT Cuts at: 1563 4563 4936 1563 Size: 3000 373 3063 Fragments arranged by size: 3063 3000 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2327 2477 4499 5883 6151 1632 Size: 477 218 150 2022 1384 268 1917 Fragments arranged by size: 2022 1917 1384 477 268 218 150 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6239 AflIII A'CryG_T Cuts at: 1203 2484 5335 6312 1203 Size: 1281 2851 977 1327 Fragments arranged by size: 2851 1327 1281 977 AgeI A'CCGG_T Cuts at: 5267 5267 Size: 6436 AhdI GACnn_n'nnGTC Cuts at: 2361 5165 2361 Size: 2804 3632 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6436 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6436 AlwNI CAG_nnn'CTG Cuts at: 2792 2804 3419 4179 5751 2792 Size: 12 615 760 1572 3477 Fragments arranged by size: 3477 1572 760 615 12 ApaLI G'TGCA_C Cuts at: 4493 5649 4493 Size: 1156 5280 ApoI r'AATT_y Cuts at: 1493 2246 2571 3745 3971 4123 6232 1493 Size: 753 325 1174 226 152 2109 1697 Fragments arranged by size: 2109 1697 1174 753 325 226 152 AseI AT'TA_AT Cuts at: 160 1032 4990 160 Size: 872 3958 1606 Fragments arranged by size: 3958 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 AvaI C'yCGr_G Cuts at: 1870 1943 2958 3137 3225 3255 3345 3578 3761 Size: 73 1015 179 88 30 90 233 183 Cuts at: 3761 1870 Size: 4545 Fragments arranged by size: 4545 1015 233 183 179 90 88 73 30 BamHI G'GATC_C Cuts at: 2551 2651 2930 3220 3494 3766 4133 2551 Size: 100 279 290 274 272 367 4854 Fragments arranged by size: 4854 367 290 279 274 272 100 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 3757 5212 618 Size: 325 217 627 537 1433 1455 1842 Fragments arranged by size: 1842 1455 1433 627 537 325 217 BanII G_rGCy'C Cuts at: 1391 3144 3755 1391 Size: 1753 611 4072 Fragments arranged by size: 4072 1753 611 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5465 BbvCI CC'TCA_GC Cuts at: 2619 2619 Size: 6436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4627 4661 1903 Size: 34 2690 34 3678 Fragments arranged by size: 3678 2690 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3394 3701 4328 5538 3394 Size: 307 627 1210 4292 Fragments arranged by size: 4292 1210 627 307 BclI T'GATC_A Cuts at: 1548 1548 Size: 6436 BfrBI ATG'CAT Cuts at: 2716 2716 Size: 6436 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2693 3266 3564 Size: 107 122 71 631 605 1021 573 298 Cuts at: 3564 5047 136 Size: 1483 1525 Fragments arranged by size: 1525 1483 1021 631 605 573 298 122 107 71 BglII A'GATC_T Cuts at: 6431 6431 Size: 6436 BlpI GC'TnA_GC Cuts at: 3853 3853 Size: 6436 Bme1580I G_kGCm'C Cuts at: 1790 2329 4497 5653 1790 Size: 539 2168 1156 2573 Fragments arranged by size: 2573 2168 1156 539 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6436 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2692 3407 3453 Size: 416 53 83 399 24 1275 715 46 Cuts at: 3453 3503 4167 5125 442 Size: 50 664 958 1753 Fragments arranged by size: 1753 1275 958 715 664 416 399 83 53 50 46 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6436 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2847 3039 3747 5096 1239 Size: 1608 192 708 1349 2579 Fragments arranged by size: 2579 1608 1349 708 192 Bpu10I CC'TnA_GC Cuts at: 2619 2647 2818 2844 3675 2619 Size: 28 171 26 831 5380 Fragments arranged by size: 5380 831 171 28 26 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4561 5426 5724 4561 Size: 865 298 5273 Fragments arranged by size: 5273 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2019 2348 5099 882 Size: 617 520 329 2751 2219 Fragments arranged by size: 2751 2219 617 520 329 BsaAI yAC'GTr Cuts at: 493 1204 4227 493 Size: 711 3023 2702 Fragments arranged by size: 3023 2702 711 BsaBI GATnn'nnATC Cuts at: 6430 6430 Size: 6436 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4243 4625 5260 275 Size: 53 83 186 936 2710 382 635 1451 Fragments arranged by size: 2710 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2554 4869 5267 5541 5688 6099 2554 Size: 2315 398 274 147 411 2891 Fragments arranged by size: 2891 2315 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3720 3750 1178 Size: 30 165 30 2317 30 3864 Fragments arranged by size: 3864 2317 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2724 2844 1186 Size: 1538 120 4778 Fragments arranged by size: 4778 1538 120 BseYI C'CCAG_C Cuts at: 2029 2636 5639 6135 2029 Size: 607 3003 496 2330 Fragments arranged by size: 3003 2330 607 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5863 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3886 4647 5675 1540 Size: 275 186 1885 761 1028 2301 Fragments arranged by size: 2301 1885 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2263 3733 4497 4582 5653 2263 Size: 1470 764 85 1071 3046 Fragments arranged by size: 3046 1470 1071 764 85 BsmI GAATG_Cn' Cuts at: 2718 2841 2859 3453 3534 3947 4040 2718 Size: 123 18 594 81 413 93 5114 Fragments arranged by size: 5114 594 413 123 93 81 18 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2263 2329 3144 3733 3755 4497 4582 Size: 399 473 66 815 589 22 742 85 Cuts at: 4582 5653 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 815 742 589 473 399 85 66 22 BspEI T'CCGG_A Cuts at: 2554 6099 2554 Size: 3545 2891 BspHI T'CATG_A Cuts at: 3607 4325 6055 3607 Size: 718 1730 3988 Fragments arranged by size: 3988 1730 718 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3622 3791 844 Size: 2778 169 3489 Fragments arranged by size: 3489 2778 169 BsrBI CCG'CTC Cuts at: 1394 1823 4323 1394 Size: 429 2500 3507 Fragments arranged by size: 3507 2500 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3312 3342 3372 4931 5105 65 Size: 1183 2064 30 30 1559 174 1396 Fragments arranged by size: 2064 1559 1396 1183 174 30 30 BsrFI r'CCGG_y Cuts at: 1700 1810 5080 5267 1700 Size: 110 3270 187 2869 Fragments arranged by size: 3270 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6436 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6436 BssSI C'ACGA_G Cuts at: 3240 3330 4496 5508 3240 Size: 90 1166 1012 4168 Fragments arranged by size: 4168 1166 1012 90 BstAPI GCAn_nnn'nTGC Cuts at: 794 2219 794 Size: 1425 5011 BstBI TT'CG_AA Cuts at: 5240 5240 Size: 6436 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6436 BstXI CCAn_nnnn'nTGG Cuts at: 2636 3083 3397 2636 Size: 447 314 5675 Fragments arranged by size: 5675 447 314 BstYI r'GATC_y Cuts at: 1972 2551 2651 2930 3220 3494 3766 4133 4518 Size: 579 100 279 290 274 272 367 385 Cuts at: 4518 4535 5987 6073 6085 6431 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 579 385 367 346 290 279 274 272 100 86 17 12 BstZ17I GTA'TAC Cuts at: 2149 3545 2149 Size: 1396 5040 Bsu36I CC'TnA_GG Cuts at: 2367 2682 2691 2367 Size: 315 9 6112 Fragments arranged by size: 6112 315 9 BtgI C'CryG_G Cuts at: 513 1065 2004 2629 2877 2990 3261 513 Size: 552 939 625 248 113 271 3688 Fragments arranged by size: 3688 939 625 552 271 248 113 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2564 3335 3365 487 Size: 683 56 1338 771 30 3558 Fragments arranged by size: 3558 1338 771 683 56 30 BtsI GCAGTG_nn' Cuts at: 3952 4746 4766 3952 Size: 794 20 5622 Fragments arranged by size: 5622 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6436 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6401 DraI TTT'AAA Cuts at: 3702 3740 3702 Size: 38 6398 DraIII CAC_nnn'GTG Cuts at: 2219 2219 Size: 6436 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5443 809 Size: 507 4127 1802 Fragments arranged by size: 4127 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3085 3883 4772 Size: 54 1425 50 275 18 1255 798 889 Cuts at: 4772 6401 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1255 889 798 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3883 1537 Size: 275 2071 4090 Fragments arranged by size: 4090 2071 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2295 2303 4366 1353 Size: 732 210 8 2063 3423 Fragments arranged by size: 3423 2063 732 210 8 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5023 5397 5543 387 Size: 1064 138 3434 374 146 1280 Fragments arranged by size: 3434 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2327 2477 2847 3039 3747 4499 Size: 393 477 218 150 370 192 708 752 Cuts at: 4499 5096 5883 6151 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 787 752 708 597 477 393 370 268 218 192 150 EcoNI CCTnn'n_nnAGG Cuts at: 2646 2829 2646 Size: 183 6253 EcoO109I rG'GnC_Cy Cuts at: 2686 2801 3170 3431 3455 3505 2686 Size: 115 369 261 24 50 5617 Fragments arranged by size: 5617 369 261 115 50 24 EcoRI G'AATT_C Cuts at: 2246 3745 4123 2246 Size: 1499 378 4559 Fragments arranged by size: 4559 1499 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2125 2125 Size: 6436 FspI TGC'GCA Cuts at: 4942 4942 Size: 6436 HaeII r_GCGC'y Cuts at: 1678 5583 1678 Size: 3905 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2343 2347 2375 2379 3313 Size: 32 135 32 771 4 28 4 934 Cuts at: 3313 3345 5077 5109 5151 5183 1373 Size: 32 1732 32 42 32 2626 Fragments arranged by size: 2626 1732 934 771 135 42 32 32 32 32 32 28 4 4 HincII GTy'rAC Cuts at: 669 1657 1938 3780 4026 669 Size: 988 281 1842 246 3079 Fragments arranged by size: 3079 1842 988 281 246 HindIII A'AGCT_T Cuts at: 748 2745 748 Size: 1997 4439 HpaI GTT'AAC Cuts at: 4026 4026 Size: 6436 KpnI G_GTAC'C Cuts at: 3761 3761 Size: 6436 MfeI C'AATT_G Cuts at: 4035 4035 Size: 6436 MluI A'CGCG_T Cuts at: 6312 6312 Size: 6436 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2101 2830 5550 5734 1305 Size: 796 729 2720 184 2007 Fragments arranged by size: 2720 2007 796 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3087 10 Size: 54 1425 343 1255 3359 Fragments arranged by size: 3359 1425 1255 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2721 4394 4753 4912 518 Size: 648 1555 1673 359 159 2042 Fragments arranged by size: 2042 1673 1555 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2033 2789 2926 3852 4529 5677 5922 1606 Size: 427 756 137 926 677 1148 245 2120 Fragments arranged by size: 2120 1148 926 756 677 427 245 137 NcoI C'CATG_G Cuts at: 513 1065 2004 2629 2877 3261 513 Size: 552 939 625 248 384 3688 Fragments arranged by size: 3688 939 625 552 384 248 NdeI CA'TA_TG Cuts at: 387 387 Size: 6436 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6436 NotI GC'GGCC_GC Cuts at: 3883 3883 Size: 6436 NsiI A_TGCA'T Cuts at: 2718 2718 Size: 6436 NspI r_CATG'y Cuts at: 1132 1229 2488 2720 3794 5339 1132 Size: 97 1259 232 1074 1545 2229 Fragments arranged by size: 2229 1545 1259 1074 232 97 PasI CC'CwG_GG Cuts at: 1348 3490 1348 Size: 2142 4294 PciI A'CATG_T Cuts at: 2484 5335 2484 Size: 2851 3585 PflMI CCAn_nnn'nTGG Cuts at: 1108 2630 2704 2864 3419 1108 Size: 1522 74 160 555 4125 Fragments arranged by size: 4125 1522 555 160 74 PfoI T'CCnGG_A Cuts at: 2824 2824 Size: 6436 PmeI GTTT'AAAC Cuts at: 3740 3740 Size: 6436 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4505 4537 6018 6050 4505 Size: 32 1481 32 4891 Fragments arranged by size: 4891 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2801 3170 3431 3455 3505 2801 Size: 369 261 24 50 5732 Fragments arranged by size: 5732 369 261 50 24 PshAI GACnn'nnGTC Cuts at: 1613 2872 1613 Size: 1259 5177 PsiI TTA'TAA Cuts at: 4006 4006 Size: 6436 PspXI vC'TCGA_Gb Cuts at: 2958 2958 Size: 6436 PstI C_TGCA'G Cuts at: 830 3652 3788 830 Size: 2822 136 3478 Fragments arranged by size: 3478 2822 136 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6436 PvuII CAG'CTG Cuts at: 2789 2789 Size: 6436 SalI G'TCGA_C Cuts at: 1936 3778 1936 Size: 1842 4594 SanDI GG'GwC_CC Cuts at: 3455 3455 Size: 6436 SapI GCTCTTCn'nnn_ Cuts at: 2085 2295 2303 2085 Size: 210 8 6218 Fragments arranged by size: 6218 210 8 SbfI CC_TGCA'GG Cuts at: 3788 3788 Size: 6436 ScaI AGT'ACT Cuts at: 4684 6396 4684 Size: 1712 4724 SfcI C'TryA_G Cuts at: 826 1045 3648 3784 4919 5600 5791 826 Size: 219 2603 136 1135 681 191 1471 Fragments arranged by size: 2603 1471 1135 681 219 191 136 SfiI GGCCn_nnn'nGGCC Cuts at: 2693 2693 Size: 6436 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6436 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6436 SmaI CCC'GGG Cuts at: 1872 3580 3763 1872 Size: 1708 183 4545 Fragments arranged by size: 4545 1708 183 SmlI C'TyrA_G Cuts at: 820 1017 1943 2958 4540 5441 5703 820 Size: 197 926 1015 1582 901 262 1553 Fragments arranged by size: 1582 1553 1015 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4227 493 Size: 3734 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6436 SphI G_CATG'C Cuts at: 1132 3794 1132 Size: 2662 3774 SspI AAT'ATT Cuts at: 5 52 4360 5 Size: 47 4308 2081 Fragments arranged by size: 4308 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6436 StyI C'CwwG_G Cuts at: 513 1065 2004 2314 2629 2877 3198 3261 3875 Size: 552 939 310 315 248 321 63 614 Cuts at: 3875 513 Size: 3074 Fragments arranged by size: 3074 939 614 552 321 315 310 248 63 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 3082 4463 4480 4633 4818 5263 Size: 1255 1116 386 1381 17 153 185 445 Cuts at: 5263 325 Size: 1498 Fragments arranged by size: 1498 1381 1255 1116 445 386 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4682 6394 96 Size: 274 80 33 51 1443 2705 1712 138 Fragments arranged by size: 2705 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2643 2675 6396 6428 2643 Size: 32 3721 32 2651 Fragments arranged by size: 3721 2651 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6436 XbaI T'CTAG_A Cuts at: 3772 3772 Size: 6436 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2705 3151 2705 Size: 446 5990 XhoI C'TCGA_G Cuts at: 1943 2958 1943 Size: 1015 5421 XmaI C'CCGG_G Cuts at: 1870 3578 3761 1870 Size: 1708 183 4545 Fragments arranged by size: 4545 1708 183 XmnI GAAnn'nnTTC Cuts at: 4565 4565 Size: 6436 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4244 276 Size: 53 83 186 936 2710 2468 Fragments arranged by size: 2710 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI RsrII SacI SacII SexAI SfoI SrfI SwaI