################################## # NO CUT ENZYMES ( 174 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraII DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV FbaI FseI FspI HapII HgaI Hin1I HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MslI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tth111I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AflII 711 CTTAAG C^TTAAG AlwI 54 GGATC GGATC(4/5) Aor51HI 739 AGCGCT AGC^GCT AvaII 232 GGACC G^G[AT]CC BbvCI 467 CCTCAGC CCTCAGC(-5/-2) BfrI 711 CTTAAG C^TTAAG BglII 207 AGATCT A^GATCT BseRI 59 GAGGAG GAGGAG(10/8) BsmAI 707 GAGAC GTCTC(1/5) BstAPI 1015 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 257 CCACTATTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 207 AGATCT [GA]^GATC[CT] Cac8I 3 GCAAGC GC[ACGT]^[ACGT]GC CfoI 740 GCGC GCG^C DsaI 497 CCATGG C^C[GA][CT]GG Eco47III 739 AGCGCT AGC^GCT EcoT22I 326 ATGCAT ATGCA^T HaeII 739 AGCGCT [GA]GCGC^[CT] HaeIII 290 GGCC GG^CC HhaI 740 GCGC GCG^C HinP1I 740 GCGC G^CGC HincII 1000 GTTGAC GT[CT]^[GA]AC HindII 1000 GTTGAC GT[CT]^[GA]AC MaeIII 539 GTAAC ^GT[ACGT]AC MflI 207 AGATCT [GA]^GATC[CT] NcoI 497 CCATGG C^CATGG NsiI 326 ATGCAT ATGCA^T PflMI 930 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 508 GAGTC GAGTC(4/5) ScaI 31 AGTACT AGT^ACT Van91I 930 CCATGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 574 TCTAGA T^CTAGA XhoII 207 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BsrDI 639 CATTGC GCAATG(2/0) 792 CATTGC BsrI 244 ACTGG ACTGG(1/-1) 71 CCAGT Cfr13I 232 GGACC G^G[ACGT]CC 289 GGGCC EcoT14I 50 CCAAGG C^C[AT][AT]GG 497 CCATGG FauI 42 CCCGC CCCGC(4/6) 76 GCGGG MaeII 339 ACGT A^CGT 492 ACGT PvuII 470 CAGCTG CAG^CTG 809 CAGCTG Sau96I 232 GGACC G^G[ACGT]CC 289 GGGCC SfaNI 1065 GATGC GCATC(5/9) 328 GCATC SmlI 316 CTTGAG C^T[CT][GA]AG 711 CTTAAG StyI 50 CCAAGG C^C[AT][AT]GG 497 CCATGG TspRI 72 CAGTG CA[GC]TG[ACGT][ACGT]^ 179 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BbvI BfaI BsaJI BsiYI BslI BssKI BstF5I BstNI DdeI DpnII EcoRII Fnu4HI FokI HinfI ItaI MaeI MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII RsaI Sau3AI ScrFI TaqI TfiI Tru9I TseI Tsp509I TthHB8I