(Circular) (Six-base) MAPSORT of: pFN21AB6521.seq Check: 9561 from: 1 to: 5794 pFN21AB6521 5794 bp With 182 enzymes: SgfI * October 20, 2009 10:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3857 5241 5509 1632 Size: 2225 1384 268 1917 Fragments arranged by size: 2225 1917 1384 268 AfeI AGC'GCT Cuts at: 2746 2746 Size: 5794 AflII C'TTAA_G Cuts at: 820 1017 2716 820 Size: 197 1699 3898 Fragments arranged by size: 3898 1699 197 AflIII A'CryG_T Cuts at: 1203 4693 5670 1203 Size: 3490 977 1327 Fragments arranged by size: 3490 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5794 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5794 AlwNI CAG_nnn'CTG Cuts at: 3537 5109 3537 Size: 1572 4222 ApaLI G'TGCA_C Cuts at: 3851 5007 3851 Size: 1156 4638 ApoI r'AATT_y Cuts at: 1493 2377 2591 2619 3103 3329 3481 5590 1493 Size: 884 214 28 484 226 152 2109 1697 Fragments arranged by size: 2109 1697 884 484 226 214 152 28 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 3119 1870 Size: 73 1176 4545 Fragments arranged by size: 4545 1176 73 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3115 4570 618 Size: 325 217 627 1328 1455 1842 Fragments arranged by size: 1842 1455 1328 627 325 217 BanII G_rGCy'C Cuts at: 1391 3113 1391 Size: 1722 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4823 BbvCI CC'TCA_GC Cuts at: 2473 2473 Size: 5794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3985 4019 1903 Size: 34 50 34 1964 34 3678 Fragments arranged by size: 3678 1964 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BfrBI ATG'CAT Cuts at: 2333 2333 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4405 136 Size: 107 122 71 631 605 2733 1525 Fragments arranged by size: 2733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2212 5789 2212 Size: 3577 2217 BlpI GC'TnA_GC Cuts at: 3211 3211 Size: 5794 Bme1580I G_kGCm'C Cuts at: 1790 3855 5011 1790 Size: 2065 1156 2573 Fragments arranged by size: 2573 2065 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3525 4483 442 Size: 416 53 83 399 24 2108 958 1753 Fragments arranged by size: 2108 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4454 1239 Size: 3215 2579 Bpu10I CC'TnA_GC Cuts at: 2473 2481 2473 Size: 8 5786 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 3919 4784 5082 2342 Size: 1577 865 298 3054 Fragments arranged by size: 3054 1577 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3585 493 Size: 711 2381 2702 Fragments arranged by size: 2702 2381 711 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3601 3983 4618 275 Size: 53 83 186 936 2068 382 635 1451 Fragments arranged by size: 2068 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4227 4625 4899 5046 5457 4227 Size: 398 274 147 411 4564 Fragments arranged by size: 4564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2498 2528 1178 Size: 30 165 30 1095 30 4444 Fragments arranged by size: 4444 1095 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2079 1186 Size: 893 4901 BseYI C'CCAG_C Cuts at: 2801 4997 5493 2801 Size: 2196 496 3102 Fragments arranged by size: 3102 2196 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5221 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3244 4005 5033 1540 Size: 275 186 1243 761 1028 2301 Fragments arranged by size: 2301 1243 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3855 3940 5011 3855 Size: 85 1071 4638 Fragments arranged by size: 4638 1071 85 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3113 3855 3940 5011 1391 Size: 399 1323 742 85 1071 2174 Fragments arranged by size: 2174 1323 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 3683 5413 3683 Size: 1730 4064 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3149 844 Size: 2305 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3681 1394 Size: 429 1858 3507 Fragments arranged by size: 3507 1858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2643 2796 4289 4463 65 Size: 1183 1395 153 1493 174 1396 Fragments arranged by size: 1493 1396 1395 1183 174 153 BsrFI r'CCGG_y Cuts at: 1700 1810 4438 4625 1700 Size: 110 2628 187 2869 Fragments arranged by size: 2869 2628 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 3854 4866 3854 Size: 1012 4782 BstAPI GCAn_nnn'nTGC Cuts at: 794 3026 794 Size: 2232 3562 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2269 Size: 5794 BstYI r'GATC_y Cuts at: 1972 2212 3124 3491 3876 3893 5345 5431 5443 Size: 240 912 367 385 17 1452 86 12 Cuts at: 5443 5789 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 912 385 367 346 240 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2502 513 Size: 552 1437 3805 Fragments arranged by size: 3805 1437 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2190 3310 4104 4124 2190 Size: 1120 794 20 3860 Fragments arranged by size: 3860 1120 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2130 2165 529 Size: 35 1566 35 4158 Fragments arranged by size: 4158 1566 35 35 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4801 809 Size: 507 3485 1802 Fragments arranged by size: 3485 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3241 4130 5759 Size: 54 1425 50 275 18 1411 889 1629 Cuts at: 5759 8 Size: 43 Fragments arranged by size: 1629 1425 1411 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3241 1537 Size: 275 1429 4090 Fragments arranged by size: 4090 1429 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3724 1353 Size: 2371 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2418 4381 4755 4901 387 Size: 1064 138 829 1963 374 146 1280 Fragments arranged by size: 1963 1280 1064 829 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3857 4454 5241 5509 1239 Size: 393 2225 597 787 268 1524 Fragments arranged by size: 2225 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2033 2033 Size: 5794 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2748 4941 1678 Size: 1070 2193 2531 Fragments arranged by size: 2531 2193 1070 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2044 2076 2498 2530 4435 Size: 32 135 32 472 32 422 32 1905 Cuts at: 4435 4467 4509 4541 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1905 472 422 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3007 3138 3384 669 Size: 988 281 1069 131 246 3079 Fragments arranged by size: 3079 1069 988 281 246 131 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4908 5092 1305 Size: 3603 184 2007 Fragments arranged by size: 3603 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3972 Fragments arranged by size: 3972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3752 4111 4270 518 Size: 648 2586 359 159 2042 Fragments arranged by size: 2586 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2050 2477 2816 3210 3887 5035 5280 1606 Size: 444 427 339 394 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 444 427 394 339 245 NcoI C'CATG_G Cuts at: 513 1065 2502 513 Size: 552 1437 3805 Fragments arranged by size: 3805 1437 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NsiI A_TGCA'T Cuts at: 2335 2335 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 3152 4697 1132 Size: 97 1923 1545 2229 Fragments arranged by size: 2229 1923 1545 97 PasI CC'CwG_GG Cuts at: 1348 2121 1348 Size: 773 5021 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2941 1108 Size: 1833 3961 PfoI T'CCnGG_A Cuts at: 2166 2166 Size: 5794 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2334 2366 2334 Size: 32 5762 PstI C_TGCA'G Cuts at: 830 3146 830 Size: 2316 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 PvuII CAG'CTG Cuts at: 2477 2816 2477 Size: 339 5455 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 2038 4042 5754 2038 Size: 2004 1712 2078 Fragments arranged by size: 2078 2004 1712 SfcI C'TryA_G Cuts at: 826 1045 3142 4277 4958 5149 826 Size: 219 2097 1135 681 191 1471 Fragments arranged by size: 2097 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2321 2716 3898 4799 5061 820 Size: 197 926 378 395 1182 901 262 1553 Fragments arranged by size: 1553 1182 926 901 395 378 262 197 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 3152 1132 Size: 2020 3774 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5794 StyI C'CwwG_G Cuts at: 513 1065 2055 2502 3233 513 Size: 552 990 447 731 3074 Fragments arranged by size: 3074 990 731 552 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2113 3821 3838 3991 4176 4621 325 Size: 1255 533 1708 17 153 185 445 1498 Fragments arranged by size: 1708 1498 1255 533 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2036 4040 5752 Size: 274 80 33 51 1443 59 2004 1712 Cuts at: 5752 96 Size: 138 Fragments arranged by size: 2004 1712 1443 274 138 80 59 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5754 5786 5754 Size: 32 5762 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5794 XbaI T'CTAG_A Cuts at: 2579 3130 2579 Size: 551 5243 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5794 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI