################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## AclI AcsI AflII AhdI AocI Aor51HI ApoI AscI AseI AsnI AspEI AvrII BamHI BciVI BfrI BlnI BsiWI BspDI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI DraI Eam1105I Eco47III Eco81I EcoRI EcoRV EcoT22I FseI HpaI MfeI MluI MunI NdeI NruI NsiI NspV PacI PmeI PshAI PshBI Psp1406I PvuI SbfI ScaI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI XbaI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AatII 3676 GACGTC GACGT^C Acc65I 1139 GGTACC G^GTACC AccIII 4142 TCCGGA T^CCGGA AgeI 34 ACCGGT A^CCGGT Alw44I 751 GTGCAC G^TGCAC ApaLI 751 GTGCAC G^TGCAC Asp718I 1139 GGTACC G^GTACC BbeI 1927 GGCGCC GGCGC^C BcgI 198 CGAGCTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BseAI 4142 TCCGGA T^CCGGA BsmBI 4303 GAGACG CGTCTC(1/5) BsmI 4176 GAATGC GAATGC(1/-1) BspEI 4142 TCCGGA T^CCGGA BsrBI 3190 GAGCGG CCGCTC(-3/-3) BsrDI 3533 GCAATG GCAATG(2/0) CpoI 4307 CGGACCG CG^G[AT]CCG HindIII 288 AAGCTT A^AGCTT KasI 1927 GGCGCC G^GCGCC KpnI 1139 GGTACC GGTAC^C KspI 2952 CCGCGG CCGC^GG MroI 4142 TCCGGA T^CCGGA MseI 2675 TTAA T^TAA NarI 1927 GGCGCC GG^CGCC NheI 4251 GCTAGC G^CTAGC NotI 1093 GCGGCCGC GC^GGCCGC PaeR7I 196 CTCGAG C^TCGAG PinAI 34 ACCGGT A^CCGGT RsrII 4307 CGGACCG CG^G[AT]CCG SacI 2158 GAGCTC GAGCT^C SacII 2952 CCGCGG CCGC^GG SalI 2914 GTCGAC G^TCGAC SfiI 2167 GGCCGCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1927 GGCGCC GGC^GCC Tru9I 2675 TTAA T^TAA Tsp509I 1168 AATT ^AATT XhoI 196 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 460 GTCTAC GT^[AC][GT]AC 2914 GTCGAC AlwNI 619 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 799 CAGGCTCTG BclI 3419 TGATCA T^GATCA 3644 TGATCA BglI 2168 GCCGCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 3347 GCCTCAAAGGC BglII 1028 AGATCT A^GATCT 2384 AGATCT BlpI 3726 GCTGAGC GC^T[ACGT]AGC 3966 GCTCAGC Bpu1102I 3726 GCTGAGC GC^T[ACGT]AGC 3966 GCTCAGC BsaBI 1002 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3414 GATCTTGATC BsaWI 34 ACCGGT [AT]^CCGG[AT] 4142 TCCGGA BspHI 1973 TCATGA T^CATGA 3680 TCATGA BspLU11I 1601 ACATGT A^CATGT 3842 ACATGT BssSI 517 CACGAG CACGAG(-5/-1) 526 CTCGTG BstAPI 618 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 744 GCAGAAGGTGC BstEII 1122 GGTCACC G^GT[ACGT]ACC 2862 GGTGACC BstPI 1122 GGTCACC G^GT[ACGT]ACC 2862 GGTGACC CelII 3726 GCTGAGC GC^T[ACGT]AGC 3966 GCTCAGC DrdI 997 GACAAGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2905 GACGGCCCGGTC EcoO65I 1122 GGTCACC G^GT[ACGT]ACC 2862 GGTGACC FbaI 3419 TGATCA T^GATCA 3644 TGATCA MamI 1002 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3414 GATCTTGATC NaeI 1282 GCCGGC GCC^GGC 4120 GCCGGC NgoMIV 1282 GCCGGC G^CCGGC 4120 GCCGGC RcaI 1973 TCATGA T^CATGA 3680 TCATGA SexAI 86 ACCTGGT A^CC[AT]GGT 3542 ACCTGGT SphI 1058 GCATGC GCATG^C 3878 GCATGC StuI 2624 AGGCCT AGG^CCT 3215 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 140 enzymes) # ######################################### AccII AciI AcyI AfaI AflIII AluI AlwI ApaI Asp700I AspHI AspI AvaI AvaII AviII BalI BanI BanII BbrPI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflFI PflMI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SspBI StyI TaqI TfiI TseI Tsp45I TspRI Tth111I TthHB8I Van91I XcmI XhoII XmaCI XmaI XmnI