(Circular) (Six-base) MAPSORT of: pFN21AE1516.seq Check: 2650 from: 1 to: 9034 pFN21AE1516 9034 bp With 182 enzymes: SgfI * August 16, 2010 14:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5685 6844 278 Size: 53 83 186 936 4149 1159 2468 Fragments arranged by size: 4149 2468 1159 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2466 4920 6377 1937 Size: 529 2454 1457 4594 Fragments arranged by size: 4594 2454 1457 529 Acc65I G'GTAC_C Cuts at: 3144 6355 3144 Size: 3211 5823 AclI AA'CG_TT Cuts at: 1563 7161 7534 1563 Size: 5598 373 3063 Fragments arranged by size: 5598 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2369 3486 3714 4034 4252 5985 7097 8481 Size: 737 1117 228 320 218 1733 1112 1384 Cuts at: 8481 8749 1632 Size: 268 1917 Fragments arranged by size: 1917 1733 1384 1117 1112 737 320 268 228 218 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8837 AflIII A'CryG_T Cuts at: 1203 2121 2712 3606 5847 5931 7933 8910 1203 Size: 918 591 894 2241 84 2002 977 1327 Fragments arranged by size: 2241 2002 1327 977 918 894 591 84 AgeI A'CCGG_T Cuts at: 2039 7865 2039 Size: 5826 3208 AhdI GACnn_n'nnGTC Cuts at: 7763 7763 Size: 9034 AleI CACnn'nnGTG Cuts at: 1166 5916 1166 Size: 4750 4284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3902 5717 5906 6059 1184 Size: 2718 1815 189 153 4159 Fragments arranged by size: 4159 2718 1815 189 153 AlwNI CAG_nnn'CTG Cuts at: 2629 2809 6777 8349 2629 Size: 180 3968 1572 3314 Fragments arranged by size: 3968 3314 1572 180 ApaI G_GGCC'C Cuts at: 2590 3818 4156 4777 5517 2590 Size: 1228 338 621 740 6107 Fragments arranged by size: 6107 1228 740 621 338 ApaLI G'TGCA_C Cuts at: 2756 7091 8247 2756 Size: 4335 1156 3543 Fragments arranged by size: 4335 3543 1156 ApoI r'AATT_y Cuts at: 1493 6343 6569 6721 8830 1493 Size: 4850 226 152 2109 1697 Fragments arranged by size: 4850 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 7588 160 Size: 872 6556 1606 Fragments arranged by size: 6556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9034 AvaI C'yCGr_G Cuts at: 1870 1943 2201 2332 3140 3485 4166 4776 5450 Size: 73 258 131 808 345 681 610 674 Cuts at: 5450 6359 1870 Size: 909 4545 Fragments arranged by size: 4545 909 808 681 674 610 345 258 131 73 BamHI G'GATC_C Cuts at: 6364 6731 6364 Size: 367 8667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2336 3144 3313 3932 4356 Size: 325 217 627 549 808 169 619 424 Cuts at: 4356 4379 5446 5918 6272 6355 7810 618 Size: 23 1067 472 354 83 1455 1842 Fragments arranged by size: 1842 1455 1067 808 627 619 549 472 424 354 325 217 169 83 23 BanII G_rGCy'C Cuts at: 1391 2532 2590 2673 3141 3213 3818 4156 4167 Size: 1141 58 83 468 72 605 338 11 Cuts at: 4167 4205 4262 4777 4885 5517 5973 6353 1391 Size: 38 57 515 108 632 456 380 4072 Fragments arranged by size: 4072 1141 632 605 515 468 456 380 338 108 83 72 58 57 38 11 BbeI G_GCGC'C Cuts at: 3936 3936 Size: 9034 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2357 3750 4825 5321 6071 928 Size: 971 458 1393 1075 496 750 3891 Fragments arranged by size: 3891 1393 1075 971 750 496 458 BbvCI CC'TCA_GC Cuts at: 2514 4115 4991 2514 Size: 1601 876 6557 Fragments arranged by size: 6557 1601 876 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2192 2226 7225 7259 1903 Size: 34 255 34 4999 34 3678 Fragments arranged by size: 4999 3678 255 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6926 8136 6926 Size: 1210 7824 BclI T'GATC_A Cuts at: 1548 5424 5649 1548 Size: 3876 225 4933 Fragments arranged by size: 4933 3876 225 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4179 5358 7645 Size: 107 122 71 631 605 2507 1179 2287 Cuts at: 7645 136 Size: 1525 Fragments arranged by size: 2507 2287 1525 1179 631 605 122 107 71 BglII A'GATC_T Cuts at: 3033 4389 9029 3033 Size: 1356 4640 3038 Fragments arranged by size: 4640 3038 1356 BlpI GC'TnA_GC Cuts at: 5732 5972 6451 5732 Size: 240 479 8315 Fragments arranged by size: 8315 479 240 Bme1580I G_kGCm'C Cuts at: 1790 2339 2590 2760 2820 3668 3818 4156 4777 Size: 549 251 170 60 848 150 338 621 Cuts at: 4777 5140 5451 5517 5923 7095 8251 1790 Size: 363 311 66 406 1172 1156 2573 Fragments arranged by size: 2573 1172 1156 848 621 549 406 363 338 311 251 170 150 66 60 BmgBI CAC'GTC Cuts at: 1318 2581 3997 1318 Size: 1263 1416 6355 Fragments arranged by size: 6355 1416 1263 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3355 3884 6765 Size: 416 53 83 399 24 1938 529 2881 Cuts at: 6765 7723 442 Size: 958 1753 Fragments arranged by size: 2881 1938 1753 958 529 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 6260 1056 Size: 5204 3830 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3274 5242 3274 Size: 1968 7066 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 3518 5783 5959 6161 6326 7694 1239 Size: 827 1452 2265 176 202 165 1368 2579 Fragments arranged by size: 2579 2265 1452 1368 827 202 176 165 Bpu10I CC'TnA_GC Cuts at: 2291 2382 2514 4115 4991 6233 2291 Size: 91 132 1601 876 1242 5092 Fragments arranged by size: 5092 1601 1242 876 132 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3005 3170 7159 8024 8322 3005 Size: 165 3989 865 298 3717 Fragments arranged by size: 3989 3717 865 298 165 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4104 5171 5333 7697 882 Size: 617 2605 1067 162 2364 2219 Fragments arranged by size: 2605 2364 2219 1067 617 162 BsaAI yAC'GTr Cuts at: 493 1204 2122 2715 3040 5089 5365 5406 5914 Size: 711 918 593 325 2049 276 41 508 Cuts at: 5914 5926 6012 6160 6825 493 Size: 12 86 148 665 2702 Fragments arranged by size: 2702 2049 918 711 665 593 508 325 276 148 86 41 12 BsaBI GATnn'nnATC Cuts at: 3011 5423 9028 3011 Size: 2412 3605 3017 Fragments arranged by size: 3605 3017 2412 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2142 3933 5682 6841 Size: 53 83 186 936 609 1791 1749 1159 Cuts at: 6841 7223 7858 275 Size: 382 635 1451 Fragments arranged by size: 1791 1749 1451 1159 936 635 609 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 6147 7467 7865 8139 8286 8697 2039 Size: 4108 1320 398 274 147 411 2376 Fragments arranged by size: 4108 2376 1320 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2702 2732 3128 3158 4229 Size: 30 165 30 1299 30 396 30 1071 Cuts at: 4229 4259 5156 5186 5330 5360 5390 5420 1178 Size: 30 897 30 144 30 30 30 4792 Fragments arranged by size: 4792 1299 1071 897 396 165 144 30 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2390 2531 2645 3170 3173 3653 3656 4247 Size: 1204 141 114 525 3 480 3 591 Cuts at: 4247 4250 4298 4523 4775 5177 5351 5411 1186 Size: 3 48 225 252 402 174 60 4809 Fragments arranged by size: 4809 1204 591 525 480 402 252 225 174 141 114 60 48 3 3 3 BseYI C'CCAG_C Cuts at: 2564 3680 3830 4274 4564 4769 5211 5253 5799 Size: 1116 150 444 290 205 442 42 546 Cuts at: 5799 5803 8237 8733 2564 Size: 4 2434 496 2865 Fragments arranged by size: 2865 2434 1116 546 496 444 442 290 205 150 42 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 2348 2423 2472 2782 3893 3965 Size: 573 540 72 75 49 310 1111 72 Cuts at: 3965 4040 4055 4311 5047 6140 1163 Size: 75 15 256 736 1093 4057 Fragments arranged by size: 4057 1111 1093 736 573 540 310 256 75 75 72 72 49 15 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3102 3288 3712 4876 4920 5830 Size: 275 186 1101 186 424 1164 44 910 Cuts at: 5830 5988 6484 7245 8273 1540 Size: 158 496 761 1028 2301 Fragments arranged by size: 2301 1164 1101 1028 910 761 496 424 275 186 186 158 44 BsiHKAI G_wGCw'C Cuts at: 2174 2523 2720 2760 4167 6239 7095 7180 8251 Size: 349 197 40 1407 2072 856 85 1071 Cuts at: 8251 2174 Size: 2957 Fragments arranged by size: 2957 2072 1407 1071 856 349 197 85 40 BsmI GAATG_Cn' Cuts at: 6187 6545 6638 6187 Size: 358 93 8583 Fragments arranged by size: 8583 358 93 BsmBI CGTCTCn'nnnn_ Cuts at: 6302 6302 Size: 9034 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2339 2523 2532 2590 2673 2720 Size: 399 384 165 184 9 58 83 47 Cuts at: 2720 2760 2820 3141 3213 3668 3818 4156 4167 Size: 40 60 321 72 455 150 338 11 Cuts at: 4167 4205 4262 4777 4885 5140 5451 5517 5923 Size: 38 57 515 108 255 311 66 406 Cuts at: 5923 5973 6239 6353 7095 7180 8251 1391 Size: 50 266 114 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 515 455 406 399 384 338 321 311 266 255 184 165 150 114 108 85 83 72 66 60 58 57 50 47 40 38 11 9 BspEI T'CCGG_A Cuts at: 6147 8697 6147 Size: 2550 6484 BspHI T'CATG_A Cuts at: 3978 5685 6923 8653 3978 Size: 1707 1238 1730 4359 Fragments arranged by size: 4359 1730 1707 1238 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2628 4599 5315 5387 5862 6389 844 Size: 1784 1971 716 72 475 527 3489 Fragments arranged by size: 3489 1971 1784 716 527 475 72 BsrBI CCG'CTC Cuts at: 1394 1823 5197 6921 1394 Size: 429 3374 1724 3507 Fragments arranged by size: 3507 3374 1724 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5545 7529 7703 65 Size: 1183 4297 1984 174 1396 Fragments arranged by size: 4297 1984 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 3287 4871 6072 6125 7678 7865 Size: 110 229 1248 1584 1201 53 1553 187 Cuts at: 7865 1700 Size: 2869 Fragments arranged by size: 2869 1584 1553 1248 1201 229 187 110 53 BsrGI T'GTAC_A Cuts at: 96 2016 2410 5769 5844 96 Size: 1920 394 3359 75 3286 Fragments arranged by size: 3359 3286 1920 394 75 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9034 BssSI C'ACGA_G Cuts at: 2522 2531 7094 8106 2522 Size: 9 4563 1012 3450 Fragments arranged by size: 4563 3450 1012 9 BstAPI GCAn_nnn'nTGC Cuts at: 794 2629 2755 794 Size: 1835 126 7073 Fragments arranged by size: 7073 1835 126 BstBI TT'CG_AA Cuts at: 7838 7838 Size: 9034 BstEII G'GTnAC_C Cuts at: 1184 3127 4867 1184 Size: 1943 1740 5351 Fragments arranged by size: 5351 1943 1740 BstXI CCAn_nnnn'nTGG Cuts at: 2044 3124 5540 5761 2044 Size: 1080 2416 221 5317 Fragments arranged by size: 5317 2416 1080 221 BstYI r'GATC_y Cuts at: 1972 3033 4389 5049 5418 6364 6731 7116 7133 Size: 1061 1356 660 369 946 367 385 17 Cuts at: 7133 8585 8671 8683 9029 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1356 1061 946 660 385 369 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2244 2925 3132 3441 3520 3819 3921 Size: 552 1179 681 207 309 79 299 102 Cuts at: 3921 3954 4383 4899 4957 5033 6240 513 Size: 33 429 516 58 76 1207 3307 Fragments arranged by size: 3307 1207 1179 681 552 516 429 309 299 207 102 79 76 58 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3604 487 Size: 683 56 2378 5917 Fragments arranged by size: 5917 2378 683 56 BtsI GCAGTG_nn' Cuts at: 2360 3076 3953 4183 5338 5533 6550 7344 7364 Size: 716 877 230 1155 195 1017 794 20 Cuts at: 7364 2360 Size: 4030 Fragments arranged by size: 4030 1155 1017 877 794 716 230 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3808 3843 529 Size: 35 3244 35 5720 Fragments arranged by size: 5720 3244 35 35 DraI TTT'AAA Cuts at: 6338 6338 Size: 9034 DraIII CAC_nnn'GTG Cuts at: 5077 5133 5551 5077 Size: 56 418 8560 Fragments arranged by size: 8560 418 56 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3008 4916 8041 809 Size: 507 1692 1908 3125 1802 Fragments arranged by size: 3125 1908 1802 1692 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2100 2247 2697 Size: 54 1425 50 275 18 270 147 450 Cuts at: 2697 2727 3099 3216 3285 3378 3517 3709 3957 Size: 30 372 117 69 93 139 192 248 Cuts at: 3957 4033 4654 4873 4902 5112 5367 5458 5542 Size: 76 621 219 29 210 255 91 84 Cuts at: 5542 5556 5563 6481 7370 8999 8 Size: 14 7 918 889 1629 43 Fragments arranged by size: 1629 1425 918 889 621 450 372 275 270 255 248 219 210 192 147 139 117 93 91 84 76 69 54 50 43 30 29 18 14 7 EagI C'GGCC_G Cuts at: 1537 1812 3099 3285 3709 4873 6481 1537 Size: 275 1287 186 424 1164 1608 4090 Fragments arranged by size: 4090 1608 1287 1164 424 275 186 EarI CTCTTCn'nnn_ Cuts at: 1353 3243 3462 3548 4010 4271 5684 6964 1353 Size: 1890 219 86 462 261 1413 1280 3423 Fragments arranged by size: 3423 1890 1413 1280 462 261 219 86 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2022 3872 5431 7621 7995 8141 Size: 1064 138 433 1850 1559 2190 374 146 Cuts at: 8141 387 Size: 1280 Fragments arranged by size: 2190 1850 1559 1280 1064 433 374 146 138 EcoICRI GAG'CTC Cuts at: 4165 4165 Size: 9034 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2369 3486 3518 3714 4034 4252 Size: 393 434 303 1117 32 196 320 218 Cuts at: 4252 5783 5959 5985 6161 6326 7097 7694 8481 Size: 1531 176 26 176 165 771 597 787 Cuts at: 8481 8749 1239 Size: 268 1524 Fragments arranged by size: 1531 1524 1117 787 771 597 434 393 320 303 268 218 196 176 176 165 32 26 EcoNI CCTnn'n_nnAGG Cuts at: 2497 2513 4541 5387 2497 Size: 16 2028 846 6144 Fragments arranged by size: 6144 2028 846 16 EcoO109I rG'GnC_Cy Cuts at: 2106 2587 3814 3815 4152 4195 4321 4453 4584 Size: 481 1227 1 337 43 126 132 131 Cuts at: 4584 4687 4773 5376 5513 5943 2106 Size: 103 86 603 137 430 5197 Fragments arranged by size: 5197 1227 603 481 430 337 137 132 131 126 103 86 43 1 EcoRI G'AATT_C Cuts at: 6343 6721 6343 Size: 378 8656 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4634 4634 Size: 9034 FspI TGC'GCA Cuts at: 3068 5330 6191 7540 3068 Size: 2262 861 1349 4562 Fragments arranged by size: 4562 2262 1349 861 HaeII r_GCGC'y Cuts at: 1678 2449 3936 4055 4565 8181 1678 Size: 771 1487 119 510 3616 2531 Fragments arranged by size: 3616 2531 1487 771 510 119 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2702 2734 3347 3379 4244 Size: 32 135 32 1130 32 613 32 865 Cuts at: 4244 4276 5308 5330 5340 5362 5403 5435 7675 Size: 32 1032 22 10 22 41 32 2240 Cuts at: 7675 7707 7749 7781 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2240 1130 1032 865 613 135 42 41 32 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 2854 4921 4948 6378 6624 669 Size: 988 281 916 2067 27 1430 246 3079 Fragments arranged by size: 3079 2067 1430 988 916 281 246 27 HindIII A'AGCT_T Cuts at: 748 2293 748 Size: 1545 7489 HpaI GTT'AAC Cuts at: 6624 6624 Size: 9034 KasI G'GCGC_C Cuts at: 3932 3932 Size: 9034 KpnI G_GTAC'C Cuts at: 3148 6359 3148 Size: 3211 5823 MfeI C'AATT_G Cuts at: 6633 6633 Size: 9034 MluI A'CGCG_T Cuts at: 8910 8910 Size: 9034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 8148 8332 1305 Size: 6843 184 2007 Fragments arranged by size: 6843 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2249 2729 3380 4904 5114 Size: 54 1425 343 417 480 651 1524 210 Cuts at: 5114 5460 5544 10 Size: 346 84 3500 Fragments arranged by size: 3500 1524 1425 651 480 417 346 343 210 84 54 MslI CAynn'nnrTG Cuts at: 518 1166 2243 2579 3122 3920 5432 5538 5916 Size: 648 1077 336 543 798 1512 106 378 Cuts at: 5916 6992 7351 7510 518 Size: 1076 359 159 2042 Fragments arranged by size: 2042 1512 1077 1076 798 648 543 378 359 336 159 106 MspA1I CmG'CkG Cuts at: 1606 2260 3299 3376 3907 4069 4119 4450 4959 Size: 654 1039 77 531 162 50 331 509 Cuts at: 4959 5056 5253 5803 6450 7127 8275 8520 1606 Size: 97 197 550 647 677 1148 245 2120 Fragments arranged by size: 2120 1148 1039 677 654 647 550 531 509 331 245 197 162 97 77 50 NaeI GCC'GGC Cuts at: 3289 6127 3289 Size: 2838 6196 NarI GG'CG_CC Cuts at: 3933 3933 Size: 9034 NcoI C'CATG_G Cuts at: 513 1065 2244 3132 4383 4899 5033 6240 513 Size: 552 1179 888 1251 516 134 1207 3307 Fragments arranged by size: 3307 1251 1207 1179 888 552 516 134 NdeI CA'TA_TG Cuts at: 387 387 Size: 9034 NgoMIV G'CCGG_C Cuts at: 3287 6125 3287 Size: 2838 6196 NheI G'CTAG_C Cuts at: 1052 6256 1052 Size: 5204 3830 NotI GC'GGCC_GC Cuts at: 3099 6481 3099 Size: 3382 5652 NspI r_CATG'y Cuts at: 1132 1229 3067 3610 4126 4604 5303 5851 5887 Size: 97 1838 543 516 478 699 548 36 Cuts at: 5887 6392 7937 1132 Size: 505 1545 2229 Fragments arranged by size: 2229 1838 1545 699 548 543 516 505 478 97 36 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 9034 PciI A'CATG_T Cuts at: 3606 5847 7933 3606 Size: 2241 2086 4707 Fragments arranged by size: 4707 2241 2086 PflMI CCAn_nnn'nTGG Cuts at: 1108 2809 4048 5077 1108 Size: 1701 1239 1029 5065 Fragments arranged by size: 5065 1701 1239 1029 PfoI T'CCnGG_A Cuts at: 6291 6291 Size: 9034 PmeI GTTT'AAAC Cuts at: 6338 6338 Size: 9034 PmlI CAC'GTG Cuts at: 2715 5089 5406 5914 5926 6012 6160 2715 Size: 2374 317 508 12 86 148 5589 Fragments arranged by size: 5589 2374 508 317 148 86 12 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7103 7135 8616 8648 7103 Size: 32 1481 32 7489 Fragments arranged by size: 7489 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2106 4321 4453 4687 5376 5943 2106 Size: 2215 132 234 689 567 5197 Fragments arranged by size: 5197 2215 689 567 234 132 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9034 PsiI TTA'TAA Cuts at: 6604 6604 Size: 9034 PspOMI G'GGCC_C Cuts at: 2586 3814 4152 4773 5513 2586 Size: 1228 338 621 740 6107 Fragments arranged by size: 6107 1228 740 621 338 PspXI vC'TCGA_Gb Cuts at: 2201 2201 Size: 9034 PstI C_TGCA'G Cuts at: 830 2625 3207 3552 3765 5289 6093 6386 830 Size: 1795 582 345 213 1524 804 293 3478 Fragments arranged by size: 3478 1795 1524 804 582 345 293 213 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9034 PvuII CAG'CTG Cuts at: 2260 3299 3376 3907 5253 5803 2260 Size: 1039 77 531 1346 550 5491 Fragments arranged by size: 5491 1346 1039 550 531 77 RsrII CG'GwC_CG Cuts at: 6313 6313 Size: 9034 SacI G_AGCT'C Cuts at: 4167 4167 Size: 9034 SacII CC_GC'GG Cuts at: 4960 4960 Size: 9034 SalI G'TCGA_C Cuts at: 1936 4919 6376 1936 Size: 2983 1457 4594 Fragments arranged by size: 4594 2983 1457 SanDI GG'GwC_CC Cuts at: 4321 4321 Size: 9034 SapI GCTCTTCn'nnn_ Cuts at: 3243 3548 5684 3243 Size: 305 2136 6593 Fragments arranged by size: 6593 2136 305 SbfI CC_TGCA'GG Cuts at: 6386 6386 Size: 9034 ScaI AGT'ACT Cuts at: 7282 8994 7282 Size: 1712 7322 SexAI A'CCwGG_T Cuts at: 2091 5547 2091 Size: 3456 5578 SfcI C'TryA_G Cuts at: 826 1045 2621 3203 3548 3761 5285 6089 6382 Size: 219 1576 582 345 213 1524 804 293 Cuts at: 6382 7517 8198 8389 826 Size: 1135 681 191 1471 Fragments arranged by size: 1576 1524 1471 1135 804 681 582 345 293 219 213 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4179 4179 Size: 9034 SfoI GGC'GCC Cuts at: 3934 3934 Size: 9034 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9034 SmaI CCC'GGG Cuts at: 1872 2334 3142 4778 6361 1872 Size: 462 808 1636 1583 4545 Fragments arranged by size: 4545 1636 1583 808 462 SmlI C'TyrA_G Cuts at: 820 1017 1943 2201 3020 3185 7138 8039 8301 Size: 197 926 258 819 165 3953 901 262 Cuts at: 8301 820 Size: 1553 Fragments arranged by size: 3953 1553 926 901 819 262 258 197 165 SnaBI TAC'GTA Cuts at: 493 6825 493 Size: 6332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 9034 SphI G_CATG'C Cuts at: 1132 3067 5887 6392 1132 Size: 1935 2820 505 3774 Fragments arranged by size: 3774 2820 1935 505 SspI AAT'ATT Cuts at: 5 52 6958 5 Size: 47 6906 2081 Fragments arranged by size: 6906 2081 47 StuI AGG'CCT Cuts at: 1517 4631 5222 1517 Size: 3114 591 5329 Fragments arranged by size: 5329 3114 591 StyI C'CwwG_G Cuts at: 513 1065 2244 3132 3654 4083 4383 4899 5033 Size: 552 1179 888 522 429 300 516 134 Cuts at: 5033 5865 6240 6473 513 Size: 832 375 233 3074 Fragments arranged by size: 3074 1179 888 832 552 522 516 429 375 300 233 134 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2354 2525 2816 2960 4136 5906 5974 Size: 1255 774 171 291 144 1176 1770 68 Cuts at: 5974 7061 7078 7231 7416 7861 325 Size: 1087 17 153 185 445 1498 Fragments arranged by size: 1770 1498 1255 1176 1087 774 445 291 185 171 153 144 68 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2016 2058 2410 Size: 274 80 33 51 1443 39 42 352 Cuts at: 2410 3189 4968 5769 5844 7280 8992 96 Size: 779 1779 801 75 1436 1712 138 Fragments arranged by size: 1779 1712 1443 1436 801 779 352 274 138 80 75 51 42 39 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3864 3896 4064 4096 5390 5422 8994 9026 3864 Size: 32 168 32 1294 32 3572 32 3872 Fragments arranged by size: 3872 3572 1294 168 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2356 5320 5344 6317 1316 Size: 1040 2964 24 973 4033 Fragments arranged by size: 4033 2964 1040 973 24 XbaI T'CTAG_A Cuts at: 6370 6370 Size: 9034 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2257 2314 2684 2833 3439 2257 Size: 57 370 149 606 7852 Fragments arranged by size: 7852 606 370 149 57 XhoI C'TCGA_G Cuts at: 1943 2201 1943 Size: 258 8776 XmaI C'CCGG_G Cuts at: 1870 2332 3140 4776 6359 1870 Size: 462 808 1636 1583 4545 Fragments arranged by size: 4545 1636 1583 808 462 XmnI GAAnn'nnTTC Cuts at: 3471 4433 5480 5573 5933 7163 3471 Size: 962 1047 93 360 1230 5342 Fragments arranged by size: 5342 1230 1047 962 360 93 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5683 6842 276 Size: 53 83 186 936 4149 1159 2468 Fragments arranged by size: 4149 2468 1159 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PsrI SrfI SwaI