Restriction map for pFN21AE1516
 (Circular) (Six-base) MAPSORT of: pFN21AE1516.seq  Check: 2650  from: 1  to: 9034



pFN21AE1516  9034 bp

 With 182 enzymes: SgfI * 

                        August 16, 2010 14:48  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    5685    6844     278 
   Size:         53      83     186     936    4149    1159    2468

  Fragments arranged by size: 

                4149    2468    1159     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2466    4920    6377    1937 
   Size:        529    2454    1457    4594

  Fragments arranged by size: 

                4594    2454    1457     529
 
Acc65I G'GTAC_C

Cuts at:   3144    6355    3144 
   Size:       3211    5823
 
AclI AA'CG_TT

Cuts at:   1563    7161    7534    1563 
   Size:       5598     373    3063

  Fragments arranged by size: 

                5598    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2369    3486    3714    4034    4252    5985    7097    8481 
   Size:        737    1117     228     320     218    1733    1112    1384

Cuts at:   8481    8749    1632 
   Size:        268    1917

  Fragments arranged by size: 

                1917    1733    1384    1117    1112     737     320     268
                 228     218
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    8837
 
AflIII A'CryG_T

Cuts at:   1203    2121    2712    3606    5847    5931    7933    8910    1203 
   Size:        918     591     894    2241      84    2002     977    1327

  Fragments arranged by size: 

                2241    2002    1327     977     918     894     591      84
            
 
AgeI A'CCGG_T

Cuts at:   2039    7865    2039 
   Size:       5826    3208
 
AhdI GACnn_n'nnGTC

Cuts at:   7763    7763 
   Size:       9034
 
AleI CACnn'nnGTG

Cuts at:   1166    5916    1166 
   Size:       4750    4284
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3902    5717    5906    6059    1184 
   Size:       2718    1815     189     153    4159

  Fragments arranged by size: 

                4159    2718    1815     189     153
 
AlwNI CAG_nnn'CTG

Cuts at:   2629    2809    6777    8349    2629 
   Size:        180    3968    1572    3314

  Fragments arranged by size: 

                3968    3314    1572     180
 
ApaI G_GGCC'C

Cuts at:   2590    3818    4156    4777    5517    2590 
   Size:       1228     338     621     740    6107

  Fragments arranged by size: 

                6107    1228     740     621     338
 
ApaLI G'TGCA_C

Cuts at:   2756    7091    8247    2756 
   Size:       4335    1156    3543

  Fragments arranged by size: 

                4335    3543    1156
 
ApoI r'AATT_y

Cuts at:   1493    6343    6569    6721    8830    1493 
   Size:       4850     226     152    2109    1697

  Fragments arranged by size: 

                4850    2109    1697     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    7588     160 
   Size:        872    6556    1606

  Fragments arranged by size: 

                6556    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9034
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2201    2332    3140    3485    4166    4776    5450 
   Size:         73     258     131     808     345     681     610     674

Cuts at:   5450    6359    1870 
   Size:        909    4545

  Fragments arranged by size: 

                4545     909     808     681     674     610     345     258
                 131      73
 
BamHI G'GATC_C

Cuts at:   6364    6731    6364 
   Size:        367    8667
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2336    3144    3313    3932    4356 
   Size:        325     217     627     549     808     169     619     424

Cuts at:   4356    4379    5446    5918    6272    6355    7810     618 
   Size:         23    1067     472     354      83    1455    1842

  Fragments arranged by size: 

                1842    1455    1067     808     627     619     549     472
                 424     354     325     217     169      83      23
 
BanII G_rGCy'C

Cuts at:   1391    2532    2590    2673    3141    3213    3818    4156    4167 
   Size:       1141      58      83     468      72     605     338      11

Cuts at:   4167    4205    4262    4777    4885    5517    5973    6353    1391 
   Size:         38      57     515     108     632     456     380    4072

  Fragments arranged by size: 

                4072    1141     632     605     515     468     456     380
                 338     108      83      72      58      57      38      11
            
 
BbeI G_GCGC'C

Cuts at:   3936    3936 
   Size:       9034
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    2357    3750    4825    5321    6071     928 
   Size:        971     458    1393    1075     496     750    3891

  Fragments arranged by size: 

                3891    1393    1075     971     750     496     458
 
BbvCI CC'TCA_GC

Cuts at:   2514    4115    4991    2514 
   Size:       1601     876    6557

  Fragments arranged by size: 

                6557    1601     876
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    2192    2226    7225    7259    1903 
   Size:         34     255      34    4999      34    3678

  Fragments arranged by size: 

                4999    3678     255      34      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   6926    8136    6926 
   Size:       1210    7824
 
BclI T'GATC_A

Cuts at:   1548    5424    5649    1548 
   Size:       3876     225    4933

  Fragments arranged by size: 

                4933    3876     225
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    4179    5358    7645 
   Size:        107     122      71     631     605    2507    1179    2287

Cuts at:   7645     136 
   Size:       1525

  Fragments arranged by size: 

                2507    2287    1525    1179     631     605     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3033    4389    9029    3033 
   Size:       1356    4640    3038

  Fragments arranged by size: 

                4640    3038    1356
 
BlpI GC'TnA_GC

Cuts at:   5732    5972    6451    5732 
   Size:        240     479    8315

  Fragments arranged by size: 

                8315     479     240
 
Bme1580I G_kGCm'C

Cuts at:   1790    2339    2590    2760    2820    3668    3818    4156    4777 
   Size:        549     251     170      60     848     150     338     621

Cuts at:   4777    5140    5451    5517    5923    7095    8251    1790 
   Size:        363     311      66     406    1172    1156    2573

  Fragments arranged by size: 

                2573    1172    1156     848     621     549     406     363
                 338     311     251     170     150      66      60
 
BmgBI CAC'GTC

Cuts at:   1318    2581    3997    1318 
   Size:       1263    1416    6355

  Fragments arranged by size: 

                6355    1416    1263
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    3355    3884    6765 
   Size:        416      53      83     399      24    1938     529    2881

Cuts at:   6765    7723     442 
   Size:        958    1753

  Fragments arranged by size: 

                2881    1938    1753     958     529     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    6260    1056 
   Size:       5204    3830
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3274    5242    3274 
   Size:       1968    7066
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2066    3518    5783    5959    6161    6326    7694    1239 
   Size:        827    1452    2265     176     202     165    1368    2579

  Fragments arranged by size: 

                2579    2265    1452    1368     827     202     176     165
            
 
Bpu10I CC'TnA_GC

Cuts at:   2291    2382    2514    4115    4991    6233    2291 
   Size:         91     132    1601     876    1242    5092

  Fragments arranged by size: 

                5092    1601    1242     876     132      91
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3005    3170    7159    8024    8322    3005 
   Size:        165    3989     865     298    3717

  Fragments arranged by size: 

                3989    3717     865     298     165
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    4104    5171    5333    7697     882 
   Size:        617    2605    1067     162    2364    2219

  Fragments arranged by size: 

                2605    2364    2219    1067     617     162
 
BsaAI yAC'GTr

Cuts at:    493    1204    2122    2715    3040    5089    5365    5406    5914 
   Size:        711     918     593     325    2049     276      41     508

Cuts at:   5914    5926    6012    6160    6825     493 
   Size:         12      86     148     665    2702

  Fragments arranged by size: 

                2702    2049     918     711     665     593     508     325
                 276     148      86      41      12
 
BsaBI GATnn'nnATC

Cuts at:   3011    5423    9028    3011 
   Size:       2412    3605    3017

  Fragments arranged by size: 

                3605    3017    2412
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2142    3933    5682    6841 
   Size:         53      83     186     936     609    1791    1749    1159

Cuts at:   6841    7223    7858     275 
   Size:        382     635    1451

  Fragments arranged by size: 

                1791    1749    1451    1159     936     635     609     382
                 186      83      53
 
BsaWI w'CCGG_w

Cuts at:   2039    6147    7467    7865    8139    8286    8697    2039 
   Size:       4108    1320     398     274     147     411    2376

  Fragments arranged by size: 

                4108    2376    1320     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    2702    2732    3128    3158    4229 
   Size:         30     165      30    1299      30     396      30    1071

Cuts at:   4229    4259    5156    5186    5330    5360    5390    5420    1178 
   Size:         30     897      30     144      30      30      30    4792

  Fragments arranged by size: 

                4792    1299    1071     897     396     165     144      30
                  30      30      30      30      30      30      30      30
            
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    2390    2531    2645    3170    3173    3653    3656    4247 
   Size:       1204     141     114     525       3     480       3     591

Cuts at:   4247    4250    4298    4523    4775    5177    5351    5411    1186 
   Size:          3      48     225     252     402     174      60    4809

  Fragments arranged by size: 

                4809    1204     591     525     480     402     252     225
                 174     141     114      60      48       3       3       3
            
 
BseYI C'CCAG_C

Cuts at:   2564    3680    3830    4274    4564    4769    5211    5253    5799 
   Size:       1116     150     444     290     205     442      42     546

Cuts at:   5799    5803    8237    8733    2564 
   Size:          4    2434     496    2865

  Fragments arranged by size: 

                2865    2434    1116     546     496     444     442     290
                 205     150      42       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2276    2348    2423    2472    2782    3893    3965 
   Size:        573     540      72      75      49     310    1111      72

Cuts at:   3965    4040    4055    4311    5047    6140    1163 
   Size:         75      15     256     736    1093    4057

  Fragments arranged by size: 

                4057    1111    1093     736     573     540     310     256
                  75      75      72      72      49      15
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    3102    3288    3712    4876    4920    5830 
   Size:        275     186    1101     186     424    1164      44     910

Cuts at:   5830    5988    6484    7245    8273    1540 
   Size:        158     496     761    1028    2301

  Fragments arranged by size: 

                2301    1164    1101    1028     910     761     496     424
                 275     186     186     158      44
 
BsiHKAI G_wGCw'C

Cuts at:   2174    2523    2720    2760    4167    6239    7095    7180    8251 
   Size:        349     197      40    1407    2072     856      85    1071

Cuts at:   8251    2174 
   Size:       2957

  Fragments arranged by size: 

                2957    2072    1407    1071     856     349     197      85
                  40
 
BsmI GAATG_Cn'

Cuts at:   6187    6545    6638    6187 
   Size:        358      93    8583

  Fragments arranged by size: 

                8583     358      93
 
BsmBI CGTCTCn'nnnn_

Cuts at:   6302    6302 
   Size:       9034
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2174    2339    2523    2532    2590    2673    2720 
   Size:        399     384     165     184       9      58      83      47

Cuts at:   2720    2760    2820    3141    3213    3668    3818    4156    4167 
   Size:         40      60     321      72     455     150     338      11

Cuts at:   4167    4205    4262    4777    4885    5140    5451    5517    5923 
   Size:         38      57     515     108     255     311      66     406

Cuts at:   5923    5973    6239    6353    7095    7180    8251    1391 
   Size:         50     266     114     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     515     455     406     399     384
                 338     321     311     266     255     184     165     150
                 114     108      85      83      72      66      60      58
                  57      50      47      40      38      11       9
 
BspEI T'CCGG_A

Cuts at:   6147    8697    6147 
   Size:       2550    6484
 
BspHI T'CATG_A

Cuts at:   3978    5685    6923    8653    3978 
   Size:       1707    1238    1730    4359

  Fragments arranged by size: 

                4359    1730    1707    1238
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2628    4599    5315    5387    5862    6389     844 
   Size:       1784    1971     716      72     475     527    3489

  Fragments arranged by size: 

                3489    1971    1784     716     527     475      72
 
BsrBI CCG'CTC

Cuts at:   1394    1823    5197    6921    1394 
   Size:        429    3374    1724    3507

  Fragments arranged by size: 

                3507    3374    1724     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    5545    7529    7703      65 
   Size:       1183    4297    1984     174    1396

  Fragments arranged by size: 

                4297    1984    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    2039    3287    4871    6072    6125    7678    7865 
   Size:        110     229    1248    1584    1201      53    1553     187

Cuts at:   7865    1700 
   Size:       2869

  Fragments arranged by size: 

                2869    1584    1553    1248    1201     229     187     110
                  53
 
BsrGI T'GTAC_A

Cuts at:     96    2016    2410    5769    5844      96 
   Size:       1920     394    3359      75    3286

  Fragments arranged by size: 

                3359    3286    1920     394      75
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       9034
 
BssSI C'ACGA_G

Cuts at:   2522    2531    7094    8106    2522 
   Size:          9    4563    1012    3450

  Fragments arranged by size: 

                4563    3450    1012       9
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    2629    2755     794 
   Size:       1835     126    7073

  Fragments arranged by size: 

                7073    1835     126
 
BstBI TT'CG_AA

Cuts at:   7838    7838 
   Size:       9034
 
BstEII G'GTnAC_C

Cuts at:   1184    3127    4867    1184 
   Size:       1943    1740    5351

  Fragments arranged by size: 

                5351    1943    1740
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2044    3124    5540    5761    2044 
   Size:       1080    2416     221    5317

  Fragments arranged by size: 

                5317    2416    1080     221
 
BstYI r'GATC_y

Cuts at:   1972    3033    4389    5049    5418    6364    6731    7116    7133 
   Size:       1061    1356     660     369     946     367     385      17

Cuts at:   7133    8585    8671    8683    9029    1972 
   Size:       1452      86      12     346    1977

  Fragments arranged by size: 

                1977    1452    1356    1061     946     660     385     369
                 367     346      86      17      12
 
BtgI C'CryG_G

Cuts at:    513    1065    2244    2925    3132    3441    3520    3819    3921 
   Size:        552    1179     681     207     309      79     299     102

Cuts at:   3921    3954    4383    4899    4957    5033    6240     513 
   Size:         33     429     516      58      76    1207    3307

  Fragments arranged by size: 

                3307    1207    1179     681     552     516     429     309
                 299     207     102      79      76      58      33
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    3604     487 
   Size:        683      56    2378    5917

  Fragments arranged by size: 

                5917    2378     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2360    3076    3953    4183    5338    5533    6550    7344    7364 
   Size:        716     877     230    1155     195    1017     794      20

Cuts at:   7364    2360 
   Size:       4030

  Fragments arranged by size: 

                4030    1155    1017     877     794     716     230     195
                  20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       9034
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    3808    3843     529 
   Size:         35    3244      35    5720

  Fragments arranged by size: 

                5720    3244      35      35
 
DraI TTT'AAA

Cuts at:   6338    6338 
   Size:       9034
 
DraIII CAC_nnn'GTG

Cuts at:   5077    5133    5551    5077 
   Size:         56     418    8560

  Fragments arranged by size: 

                8560     418      56
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3008    4916    8041     809 
   Size:        507    1692    1908    3125    1802

  Fragments arranged by size: 

                3125    1908    1802    1692     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2100    2247    2697 
   Size:         54    1425      50     275      18     270     147     450

Cuts at:   2697    2727    3099    3216    3285    3378    3517    3709    3957 
   Size:         30     372     117      69      93     139     192     248

Cuts at:   3957    4033    4654    4873    4902    5112    5367    5458    5542 
   Size:         76     621     219      29     210     255      91      84

Cuts at:   5542    5556    5563    6481    7370    8999       8 
   Size:         14       7     918     889    1629      43

  Fragments arranged by size: 

                1629    1425     918     889     621     450     372     275
                 270     255     248     219     210     192     147     139
                 117      93      91      84      76      69      54      50
                  43      30      29      18      14       7
 
EagI C'GGCC_G

Cuts at:   1537    1812    3099    3285    3709    4873    6481    1537 
   Size:        275    1287     186     424    1164    1608    4090

  Fragments arranged by size: 

                4090    1608    1287    1164     424     275     186
 
EarI CTCTTCn'nnn_

Cuts at:   1353    3243    3462    3548    4010    4271    5684    6964    1353 
   Size:       1890     219      86     462     261    1413    1280    3423

  Fragments arranged by size: 

                3423    1890    1413    1280     462     261     219      86
            
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    2022    3872    5431    7621    7995    8141 
   Size:       1064     138     433    1850    1559    2190     374     146

Cuts at:   8141     387 
   Size:       1280

  Fragments arranged by size: 

                2190    1850    1559    1280    1064     433     374     146
                 138
 
EcoICRI GAG'CTC

Cuts at:   4165    4165 
   Size:       9034
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2066    2369    3486    3518    3714    4034    4252 
   Size:        393     434     303    1117      32     196     320     218

Cuts at:   4252    5783    5959    5985    6161    6326    7097    7694    8481 
   Size:       1531     176      26     176     165     771     597     787

Cuts at:   8481    8749    1239 
   Size:        268    1524

  Fragments arranged by size: 

                1531    1524    1117     787     771     597     434     393
                 320     303     268     218     196     176     176     165
                  32      26
 
EcoNI CCTnn'n_nnAGG

Cuts at:   2497    2513    4541    5387    2497 
   Size:         16    2028     846    6144

  Fragments arranged by size: 

                6144    2028     846      16
 
EcoO109I rG'GnC_Cy

Cuts at:   2106    2587    3814    3815    4152    4195    4321    4453    4584 
   Size:        481    1227       1     337      43     126     132     131

Cuts at:   4584    4687    4773    5376    5513    5943    2106 
   Size:        103      86     603     137     430    5197

  Fragments arranged by size: 

                5197    1227     603     481     430     337     137     132
                 131     126     103      86      43       1
 
EcoRI G'AATT_C

Cuts at:   6343    6721    6343 
   Size:        378    8656
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   4634    4634 
   Size:       9034
 
FspI TGC'GCA

Cuts at:   3068    5330    6191    7540    3068 
   Size:       2262     861    1349    4562

  Fragments arranged by size: 

                4562    2262    1349     861
 
HaeII r_GCGC'y

Cuts at:   1678    2449    3936    4055    4565    8181    1678 
   Size:        771    1487     119     510    3616    2531

  Fragments arranged by size: 

                3616    2531    1487     771     510     119
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2702    2734    3347    3379    4244 
   Size:         32     135      32    1130      32     613      32     865

Cuts at:   4244    4276    5308    5330    5340    5362    5403    5435    7675 
   Size:         32    1032      22      10      22      41      32    2240

Cuts at:   7675    7707    7749    7781    1373 
   Size:         32      42      32    2626

  Fragments arranged by size: 

                2626    2240    1130    1032     865     613     135      42
                  41      32      32      32      32      32      32      32
                  32      22      22      10
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2854    4921    4948    6378    6624     669 
   Size:        988     281     916    2067      27    1430     246    3079

  Fragments arranged by size: 

                3079    2067    1430     988     916     281     246      27
            
 
HindIII A'AGCT_T

Cuts at:    748    2293     748 
   Size:       1545    7489
 
HpaI GTT'AAC

Cuts at:   6624    6624 
   Size:       9034
 
KasI G'GCGC_C

Cuts at:   3932    3932 
   Size:       9034
 
KpnI G_GTAC'C

Cuts at:   3148    6359    3148 
   Size:       3211    5823
 
MfeI C'AATT_G

Cuts at:   6633    6633 
   Size:       9034
 
MluI A'CGCG_T

Cuts at:   8910    8910 
   Size:       9034
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    8148    8332    1305 
   Size:       6843     184    2007

  Fragments arranged by size: 

                6843    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2249    2729    3380    4904    5114 
   Size:         54    1425     343     417     480     651    1524     210

Cuts at:   5114    5460    5544      10 
   Size:        346      84    3500

  Fragments arranged by size: 

                3500    1524    1425     651     480     417     346     343
                 210      84      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2243    2579    3122    3920    5432    5538    5916 
   Size:        648    1077     336     543     798    1512     106     378

Cuts at:   5916    6992    7351    7510     518 
   Size:       1076     359     159    2042

  Fragments arranged by size: 

                2042    1512    1077    1076     798     648     543     378
                 359     336     159     106
 
MspA1I CmG'CkG

Cuts at:   1606    2260    3299    3376    3907    4069    4119    4450    4959 
   Size:        654    1039      77     531     162      50     331     509

Cuts at:   4959    5056    5253    5803    6450    7127    8275    8520    1606 
   Size:         97     197     550     647     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148    1039     677     654     647     550     531
                 509     331     245     197     162      97      77      50
            
 
NaeI GCC'GGC

Cuts at:   3289    6127    3289 
   Size:       2838    6196
 
NarI GG'CG_CC

Cuts at:   3933    3933 
   Size:       9034
 
NcoI C'CATG_G

Cuts at:    513    1065    2244    3132    4383    4899    5033    6240     513 
   Size:        552    1179     888    1251     516     134    1207    3307

  Fragments arranged by size: 

                3307    1251    1207    1179     888     552     516     134
            
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       9034
 
NgoMIV G'CCGG_C

Cuts at:   3287    6125    3287 
   Size:       2838    6196
 
NheI G'CTAG_C

Cuts at:   1052    6256    1052 
   Size:       5204    3830
 
NotI GC'GGCC_GC

Cuts at:   3099    6481    3099 
   Size:       3382    5652
 
NspI r_CATG'y

Cuts at:   1132    1229    3067    3610    4126    4604    5303    5851    5887 
   Size:         97    1838     543     516     478     699     548      36

Cuts at:   5887    6392    7937    1132 
   Size:        505    1545    2229

  Fragments arranged by size: 

                2229    1838    1545     699     548     543     516     505
                 478      97      36
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       9034
 
PciI A'CATG_T

Cuts at:   3606    5847    7933    3606 
   Size:       2241    2086    4707

  Fragments arranged by size: 

                4707    2241    2086
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    2809    4048    5077    1108 
   Size:       1701    1239    1029    5065

  Fragments arranged by size: 

                5065    1701    1239    1029
 
PfoI T'CCnGG_A

Cuts at:   6291    6291 
   Size:       9034
 
PmeI GTTT'AAAC

Cuts at:   6338    6338 
   Size:       9034
 
PmlI CAC'GTG

Cuts at:   2715    5089    5406    5914    5926    6012    6160    2715 
   Size:       2374     317     508      12      86     148    5589

  Fragments arranged by size: 

                5589    2374     508     317     148      86      12
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   7103    7135    8616    8648    7103 
   Size:         32    1481      32    7489

  Fragments arranged by size: 

                7489    1481      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   2106    4321    4453    4687    5376    5943    2106 
   Size:       2215     132     234     689     567    5197

  Fragments arranged by size: 

                5197    2215     689     567     234     132
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       9034
 
PsiI TTA'TAA

Cuts at:   6604    6604 
   Size:       9034
 
PspOMI G'GGCC_C

Cuts at:   2586    3814    4152    4773    5513    2586 
   Size:       1228     338     621     740    6107

  Fragments arranged by size: 

                6107    1228     740     621     338
 
PspXI vC'TCGA_Gb

Cuts at:   2201    2201 
   Size:       9034
 
PstI C_TGCA'G

Cuts at:    830    2625    3207    3552    3765    5289    6093    6386     830 
   Size:       1795     582     345     213    1524     804     293    3478

  Fragments arranged by size: 

                3478    1795    1524     804     582     345     293     213
            
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       9034
 
PvuII CAG'CTG

Cuts at:   2260    3299    3376    3907    5253    5803    2260 
   Size:       1039      77     531    1346     550    5491

  Fragments arranged by size: 

                5491    1346    1039     550     531      77
 
RsrII CG'GwC_CG

Cuts at:   6313    6313 
   Size:       9034
 
SacI G_AGCT'C

Cuts at:   4167    4167 
   Size:       9034
 
SacII CC_GC'GG

Cuts at:   4960    4960 
   Size:       9034
 
SalI G'TCGA_C

Cuts at:   1936    4919    6376    1936 
   Size:       2983    1457    4594

  Fragments arranged by size: 

                4594    2983    1457
 
SanDI GG'GwC_CC

Cuts at:   4321    4321 
   Size:       9034
 
SapI GCTCTTCn'nnn_

Cuts at:   3243    3548    5684    3243 
   Size:        305    2136    6593

  Fragments arranged by size: 

                6593    2136     305
 
SbfI CC_TGCA'GG

Cuts at:   6386    6386 
   Size:       9034
 
ScaI AGT'ACT

Cuts at:   7282    8994    7282 
   Size:       1712    7322
 
SexAI A'CCwGG_T

Cuts at:   2091    5547    2091 
   Size:       3456    5578
 
SfcI C'TryA_G

Cuts at:    826    1045    2621    3203    3548    3761    5285    6089    6382 
   Size:        219    1576     582     345     213    1524     804     293

Cuts at:   6382    7517    8198    8389     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1576    1524    1471    1135     804     681     582     345
                 293     219     213     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:   4179    4179 
   Size:       9034
 
SfoI GGC'GCC

Cuts at:   3934    3934 
   Size:       9034
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       9034
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       9034
 
SmaI CCC'GGG

Cuts at:   1872    2334    3142    4778    6361    1872 
   Size:        462     808    1636    1583    4545

  Fragments arranged by size: 

                4545    1636    1583     808     462
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2201    3020    3185    7138    8039    8301 
   Size:        197     926     258     819     165    3953     901     262

Cuts at:   8301     820 
   Size:       1553

  Fragments arranged by size: 

                3953    1553     926     901     819     262     258     197
                 165
 
SnaBI TAC'GTA

Cuts at:    493    6825     493 
   Size:       6332    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       9034
 
SphI G_CATG'C

Cuts at:   1132    3067    5887    6392    1132 
   Size:       1935    2820     505    3774

  Fragments arranged by size: 

                3774    2820    1935     505
 
SspI AAT'ATT

Cuts at:      5      52    6958       5 
   Size:         47    6906    2081

  Fragments arranged by size: 

                6906    2081      47
 
StuI AGG'CCT

Cuts at:   1517    4631    5222    1517 
   Size:       3114     591    5329

  Fragments arranged by size: 

                5329    3114     591
 
StyI C'CwwG_G

Cuts at:    513    1065    2244    3132    3654    4083    4383    4899    5033 
   Size:        552    1179     888     522     429     300     516     134

Cuts at:   5033    5865    6240    6473     513 
   Size:        832     375     233    3074

  Fragments arranged by size: 

                3074    1179     888     832     552     522     516     429
                 375     300     233     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2354    2525    2816    2960    4136    5906    5974 
   Size:       1255     774     171     291     144    1176    1770      68

Cuts at:   5974    7061    7078    7231    7416    7861     325 
   Size:       1087      17     153     185     445    1498

  Fragments arranged by size: 

                1770    1498    1255    1176    1087     774     445     291
                 185     171     153     144      68      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    2016    2058    2410 
   Size:        274      80      33      51    1443      39      42     352

Cuts at:   2410    3189    4968    5769    5844    7280    8992      96 
   Size:        779    1779     801      75    1436    1712     138

  Fragments arranged by size: 

                1779    1712    1443    1436     801     779     352     274
                 138      80      75      51      42      39      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3864    3896    4064    4096    5390    5422    8994    9026    3864 
   Size:         32     168      32    1294      32    3572      32    3872

  Fragments arranged by size: 

                3872    3572    1294     168      32      32      32      32
            
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2356    5320    5344    6317    1316 
   Size:       1040    2964      24     973    4033

  Fragments arranged by size: 

                4033    2964    1040     973      24
 
XbaI T'CTAG_A

Cuts at:   6370    6370 
   Size:       9034
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2257    2314    2684    2833    3439    2257 
   Size:         57     370     149     606    7852

  Fragments arranged by size: 

                7852     606     370     149      57
 
XhoI C'TCGA_G

Cuts at:   1943    2201    1943 
   Size:        258    8776
 
XmaI C'CCGG_G

Cuts at:   1870    2332    3140    4776    6359    1870 
   Size:        462     808    1636    1583    4545

  Fragments arranged by size: 

                4545    1636    1583     808     462
 
XmnI GAAnn'nnTTC

Cuts at:   3471    4433    5480    5573    5933    7163    3471 
   Size:        962    1047      93     360    1230    5342

  Fragments arranged by size: 

                5342    1230    1047     962     360      93
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    5683    6842     276 
   Size:         53      83     186     936    4149    1159    2468

  Fragments arranged by size: 

                4149    2468    1159     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI     ApaI    ApaLI     ApoI     AseI
    AsiSI     AvaI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NspI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PstI     PvuI
    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     AscI    AvrII     BaeI    BfrBI    BsiWI
  BstZ17I   Bsu36I    EcoRV     FseI    FspAI     NruI     NsiI     PacI
     PsrI     SrfI     SwaI
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