################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccIII AclI AcsI AcyI AflII AflIII AhdI Alw44I AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrGI BssHII Bst1107I BstBI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI Ksp632I KspI MamI MfeI MflI MluI MluNI MroI MscI MslI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I XbaI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 766 GGTACC G^GTACC AccI 301 GTCTAC GT^[AC][GT]AC AgeI 113 ACCGGT A^CCGGT Asp718I 766 GGTACC G^GTACC AvaI 12 CCCGGG C^[CT]CG[GA]G BciVI 292 GTATCC GTATCC(6/5) BfaI 410 CTAG C^TAG BsaI 343 GGTCTC GGTCTC(1/5) BsaWI 113 ACCGGT [AT]^CCGG[AT] BsgI 97 CTGCAC GTGCAG(16/14) BsmBI 475 CGTCTC CGTCTC(1/5) BsoBI 12 CCCGGG C^[CT]CG[GA]G BsrDI 867 CATTGC GCAATG(2/0) BsrFI 113 ACCGGT [GA]^CCGG[CT] BssSI 947 CACGAG CACGAG(-5/-1) BstAPI 742 GCAGTGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 24 GGTGACC G^GT[ACGT]ACC BstPI 24 GGTGACC G^GT[ACGT]ACC CfoI 70 GCGC GCG^C Cfr10I 113 ACCGGT [GA]^CCGG[CT] EaeI 958 CGGCCA [CT]^GGCC[GA] EcoO65I 24 GGTGACC G^GT[ACGT]ACC EcoRV 850 GATATC GAT^ATC HgaI 711 GACGC GACGC(5/10) HhaI 70 GCGC GCG^C HinP1I 70 GCGC G^CGC KpnI 766 GGTACC GGTAC^C MaeI 410 CTAG C^TAG MaeII 506 ACGT A^CGT MseI 625 TTAA T^TAA PflMI 961 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 113 ACCGGT A^CCGGT PpuMI 922 GGGACCC [GA]G^G[AT]CC[CT] PstI 740 CTGCAG CTGCA^G SacI 897 GAGCTC GAGCT^C SmaI 12 CCCGGG CCC^GGG SmlI 1107 CTTGAG C^T[CT][GA]AG StuI 979 AGGCCT AGG^CCT Tru9I 625 TTAA T^TAA Van91I 961 CCACCCCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1146 CCAGAGTCAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 12 CCCGGG C^CCGGG XmaI 12 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AccII 69 CGCG CG^CG 80 CGCG AlwI 243 GGATC GGATC(4/5) 561 GGATC AlwNI 728 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1018 CAGGCCCTG AspHI 723 GAGCAC G[AT]GC[AT]^C 897 GAGCTC BbsI 180 GTCTTC GAAGAC(2/6) 645 GTCTTC BpuAI 180 GTCTTC GAAGAC(2/6) 645 GTCTTC BseRI 461 CTCCTC GAGGAG(10/8) 835 GAGGAG BsiHKAI 723 GAGCAC G[AT]GC[AT]^C 897 GAGCTC BsmFI 922 GGGAC GGGAC(10/14) 327 GTCCC BstUI 69 CGCG CG^CG 80 CGCG DpnII 244 GATC ^GATC 562 GATC MboI 244 GATC ^GATC 562 GATC MvnI 69 CGCG CG^CG 80 CGCG NdeII 244 GATC ^GATC 562 GATC PvuII 88 CAGCTG CAG^CTG 372 CAGCTG Sau3AI 244 GATC ^GATC 562 GATC SfaNI 862 GATGC GCATC(5/9) 422 GCATC SfcI 369 CTACAG C^T[GA][CT]AG 740 CTGCAG ######################################### # 3 OR MORE CUT ENZYMES ( 56 enzymes) # ######################################### AciI AfaI AluI ApaI AvaII BanI BanII BbvI BcnI BmyI BpmI BsaJI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DraII DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboII MnlI MspA1I MspI MvaI MwoI NciI NcoI NlaIII NlaIV PleI RsaI Sau96I ScrFI StyI TseI Tsp45I TspRI