(Circular) (Six-base) MAPSORT of: pFN21AE6330.seq Check: 8328 from: 1 to: 5875 pFN21AE6330 5875 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3685 278 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2307 3218 1937 Size: 370 911 4594 Fragments arranged by size: 4594 911 370 Acc65I G'GTAC_C Cuts at: 2771 3196 2771 Size: 425 5450 AclI AA'CG_TT Cuts at: 1563 4002 4375 1563 Size: 2439 373 3063 Fragments arranged by size: 3063 2439 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3938 5322 5590 1632 Size: 2306 1384 268 1917 Fragments arranged by size: 2306 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5678 AflIII A'CryG_T Cuts at: 1203 4774 5751 1203 Size: 3571 977 1327 Fragments arranged by size: 3571 1327 977 AgeI A'CCGG_T Cuts at: 2118 4706 2118 Size: 2588 3287 AhdI GACnn_n'nnGTC Cuts at: 4604 4604 Size: 5875 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5875 AlwNI CAG_nnn'CTG Cuts at: 2738 3028 3618 5190 2738 Size: 290 590 1572 3423 Fragments arranged by size: 3423 1572 590 290 ApaI G_GGCC'C Cuts at: 2025 2092 3064 2025 Size: 67 972 4836 Fragments arranged by size: 4836 972 67 ApaLI G'TGCA_C Cuts at: 3932 5088 3932 Size: 1156 4719 ApoI r'AATT_y Cuts at: 1493 3184 3410 3562 5671 1493 Size: 1691 226 152 2109 1697 Fragments arranged by size: 2109 1697 1691 226 152 AseI AT'TA_AT Cuts at: 160 1032 4429 160 Size: 872 3397 1606 Fragments arranged by size: 3397 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 AvaI C'yCGr_G Cuts at: 1870 1943 2017 3200 1870 Size: 73 74 1183 4545 Fragments arranged by size: 4545 1183 74 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2763 2796 2763 Size: 33 5842 BamHI G'GATC_C Cuts at: 3205 3572 3205 Size: 367 5508 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2206 2771 3142 3196 4651 Size: 325 217 627 419 565 371 54 1455 Cuts at: 4651 618 Size: 1842 Fragments arranged by size: 1842 1455 627 565 419 371 325 217 54 BanII G_rGCy'C Cuts at: 1391 2025 2092 2906 2959 3064 3194 1391 Size: 634 67 814 53 105 130 4072 Fragments arranged by size: 4072 814 634 130 105 67 53 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2178 2643 928 Size: 971 279 465 4160 Fragments arranged by size: 4160 971 465 279 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4066 4100 1903 Size: 34 2129 34 3678 Fragments arranged by size: 3678 2129 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2308 3767 4977 2308 Size: 1459 1210 3206 Fragments arranged by size: 3206 1459 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5875 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4486 136 Size: 107 122 71 631 605 2814 1525 Fragments arranged by size: 2814 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5870 5870 Size: 5875 BlpI GC'TnA_GC Cuts at: 3292 3292 Size: 5875 Bme1580I G_kGCm'C Cuts at: 1790 2025 2071 2092 2211 2496 2699 3064 3936 Size: 235 46 21 119 285 203 365 872 Cuts at: 3936 5092 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 872 365 285 235 203 119 46 21 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5875 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2795 3003 3606 Size: 416 53 83 399 24 1378 208 603 Cuts at: 3606 4564 442 Size: 958 1753 Fragments arranged by size: 1753 1378 958 603 416 399 208 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5875 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2380 2380 Size: 5875 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2313 2336 2363 2445 2468 2682 2760 2778 Size: 1074 23 27 82 23 214 78 18 Cuts at: 2778 2858 3001 4535 1239 Size: 80 143 1534 2579 Fragments arranged by size: 2579 1534 1074 214 143 82 80 78 27 23 23 18 Bpu10I CC'TnA_GC Cuts at: 2519 2519 Size: 5875 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3133 4000 4865 5163 3133 Size: 867 865 298 3845 Fragments arranged by size: 3845 867 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2354 4538 882 Size: 617 855 2184 2219 Fragments arranged by size: 2219 2184 855 617 BsaAI yAC'GTr Cuts at: 493 1204 3666 493 Size: 711 2462 2702 Fragments arranged by size: 2702 2462 711 BsaBI GATnn'nnATC Cuts at: 5869 5869 Size: 5875 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3682 4064 4699 275 Size: 53 83 186 936 2149 382 635 1451 Fragments arranged by size: 2149 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2118 4308 4706 4980 5127 5538 2118 Size: 2190 398 274 147 411 2455 Fragments arranged by size: 2455 2190 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2130 2160 2366 2396 2655 Size: 30 165 30 727 30 206 30 259 Cuts at: 2655 2685 2733 2751 2763 2781 2900 2930 2943 Size: 30 48 18 12 18 119 30 13 Cuts at: 2943 2973 1178 Size: 30 4080 Fragments arranged by size: 4080 727 259 206 165 119 48 30 30 30 30 30 30 30 18 18 13 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2457 2855 1186 Size: 1271 398 4206 Fragments arranged by size: 4206 1271 398 BseYI C'CCAG_C Cuts at: 2091 2193 2798 5078 5574 2091 Size: 102 605 2280 496 2392 Fragments arranged by size: 2392 2280 605 496 102 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2087 1163 Size: 573 351 4951 Fragments arranged by size: 4951 573 351 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3325 4086 5114 1540 Size: 275 186 1324 761 1028 2301 Fragments arranged by size: 2301 1324 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2732 2906 3936 4021 5092 2732 Size: 174 1030 85 1071 3515 Fragments arranged by size: 3515 1071 1030 174 85 BsmI GAATG_Cn' Cuts at: 3386 3479 3386 Size: 93 5782 BsmBI CGTCTCn'nnnn_ Cuts at: 2486 2486 Size: 5875 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2071 2092 2211 2496 2699 2732 Size: 399 235 46 21 119 285 203 33 Cuts at: 2732 2906 2959 3064 3194 3936 4021 5092 1391 Size: 174 53 105 130 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 285 235 203 174 130 119 105 85 53 46 33 21 BspEI T'CCGG_A Cuts at: 5538 5538 Size: 5875 BspHI T'CATG_A Cuts at: 3764 5494 3764 Size: 1730 4145 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3230 844 Size: 2386 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3762 1394 Size: 429 1939 3507 Fragments arranged by size: 3507 1939 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2871 4370 4544 65 Size: 1183 1623 1499 174 1396 Fragments arranged by size: 1623 1499 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2118 4519 4706 1700 Size: 110 308 2401 187 2869 Fragments arranged by size: 2869 2401 308 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5875 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5875 BssSI C'ACGA_G Cuts at: 2952 3935 4947 2952 Size: 983 1012 3880 Fragments arranged by size: 3880 1012 983 BstAPI GCAn_nnn'nTGC Cuts at: 794 2753 794 Size: 1959 3916 BstBI TT'CG_AA Cuts at: 4679 4679 Size: 5875 BstEII G'GTnAC_C Cuts at: 1184 2029 1184 Size: 845 5030 BstYI r'GATC_y Cuts at: 1972 3205 3572 3957 3974 5426 5512 5524 5870 Size: 1233 367 385 17 1452 86 12 346 Cuts at: 5870 1972 Size: 1977 Fragments arranged by size: 1977 1452 1233 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2198 2620 2655 2971 513 Size: 552 1133 422 35 316 3417 Fragments arranged by size: 3417 1133 552 422 316 35 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5136 Fragments arranged by size: 5136 683 56 BtsI GCAGTG_nn' Cuts at: 2697 2754 3391 4185 4205 2697 Size: 57 637 794 20 4367 Fragments arranged by size: 4367 794 637 57 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5875 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2543 2578 529 Size: 35 1979 35 3826 Fragments arranged by size: 3826 1979 35 35 DraI TTT'AAA Cuts at: 3179 3179 Size: 5875 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4882 809 Size: 507 3566 1802 Fragments arranged by size: 3566 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2963 3322 4211 Size: 54 1425 50 275 18 1133 359 889 Cuts at: 4211 5840 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1133 889 359 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3322 1537 Size: 275 1510 4090 Fragments arranged by size: 4090 1510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3805 1353 Size: 2452 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4462 4836 4982 387 Size: 1064 138 2873 374 146 1280 Fragments arranged by size: 2873 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2904 2904 Size: 5875 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2313 2336 2363 2445 2468 2682 2760 Size: 393 681 23 27 82 23 214 78 Cuts at: 2760 2778 2858 3001 3938 4535 5322 5590 1239 Size: 18 80 143 937 597 787 268 1524 Fragments arranged by size: 1524 937 787 681 597 393 268 214 143 82 80 78 27 23 23 18 EcoO109I rG'GnC_Cy Cuts at: 2021 2022 2735 2833 2928 3025 3060 2021 Size: 1 713 98 95 97 35 4836 Fragments arranged by size: 4836 713 98 97 95 35 1 EcoRI G'AATT_C Cuts at: 3184 3562 3184 Size: 378 5497 EcoRV GAT'ATC Cuts at: 2857 2857 Size: 5875 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2168 2168 Size: 5875 FspI TGC'GCA Cuts at: 4381 4381 Size: 5875 HaeII r_GCGC'y Cuts at: 1678 5022 1678 Size: 3344 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2655 2687 2900 2932 4516 Size: 32 135 32 1083 32 213 32 1584 Cuts at: 4516 4548 4590 4622 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1584 1083 213 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3219 3465 669 Size: 988 281 1281 246 3079 Fragments arranged by size: 3079 1281 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5875 HpaI GTT'AAC Cuts at: 3465 3465 Size: 5875 KpnI G_GTAC'C Cuts at: 2775 3200 2775 Size: 425 5450 MfeI C'AATT_G Cuts at: 3474 3474 Size: 5875 MluI A'CGCG_T Cuts at: 5751 5751 Size: 5875 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2761 4989 5173 1305 Size: 1456 2228 184 2007 Fragments arranged by size: 2228 2007 1456 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4053 Fragments arranged by size: 4053 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3833 4192 4351 518 Size: 648 2667 359 159 2042 Fragments arranged by size: 2667 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2095 2379 2549 3291 3968 5116 5361 1606 Size: 489 284 170 742 677 1148 245 2120 Fragments arranged by size: 2120 1148 742 677 489 284 245 170 NcoI C'CATG_G Cuts at: 513 1065 2198 2655 2971 513 Size: 552 1133 457 316 3417 Fragments arranged by size: 3417 1133 552 457 316 NdeI CA'TA_TG Cuts at: 387 387 Size: 5875 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5875 NotI GC'GGCC_GC Cuts at: 3322 3322 Size: 5875 NspI r_CATG'y Cuts at: 1132 1229 3233 4778 1132 Size: 97 2004 1545 2229 Fragments arranged by size: 2229 2004 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5875 PciI A'CATG_T Cuts at: 4774 4774 Size: 5875 PflMI CCAn_nnn'nTGG Cuts at: 1108 2972 1108 Size: 1864 4011 PfoI T'CCnGG_A Cuts at: 2321 2352 2321 Size: 31 5844 PmeI GTTT'AAAC Cuts at: 3179 3179 Size: 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3944 3976 5457 5489 3944 Size: 32 1481 32 4330 Fragments arranged by size: 4330 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2928 2928 Size: 5875 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5875 PsiI TTA'TAA Cuts at: 3445 3445 Size: 5875 PspOMI G'GGCC_C Cuts at: 2021 2088 3060 2021 Size: 67 972 4836 Fragments arranged by size: 4836 972 67 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2433 2465 2433 Size: 32 5843 PstI C_TGCA'G Cuts at: 830 2749 3227 830 Size: 1919 478 3478 Fragments arranged by size: 3478 1919 478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5875 PvuII CAG'CTG Cuts at: 2095 2379 2095 Size: 284 5591 SacI G_AGCT'C Cuts at: 2906 2906 Size: 5875 SalI G'TCGA_C Cuts at: 1936 3217 1936 Size: 1281 4594 SanDI GG'GwC_CC Cuts at: 2928 2928 Size: 5875 SbfI CC_TGCA'GG Cuts at: 3227 3227 Size: 5875 ScaI AGT'ACT Cuts at: 4123 5835 4123 Size: 1712 4163 SfcI C'TryA_G Cuts at: 826 1045 2374 2745 3223 4358 5039 5230 826 Size: 219 1329 371 478 1135 681 191 1471 Fragments arranged by size: 1471 1329 1135 681 478 371 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5875 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5875 SmaI CCC'GGG Cuts at: 1872 2019 3202 1872 Size: 147 1183 4545 Fragments arranged by size: 4545 1183 147 SmlI C'TyrA_G Cuts at: 820 1017 1943 3112 3979 4880 5142 820 Size: 197 926 1169 867 901 262 1553 Fragments arranged by size: 1553 1169 926 901 867 262 197 SnaBI TAC'GTA Cuts at: 493 3666 493 Size: 3173 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5875 SphI G_CATG'C Cuts at: 1132 3233 1132 Size: 2101 3774 SspI AAT'ATT Cuts at: 5 52 3799 5 Size: 47 3747 2081 Fragments arranged by size: 3747 2081 47 StuI AGG'CCT Cuts at: 1517 2986 1517 Size: 1469 4406 StyI C'CwwG_G Cuts at: 513 1065 2198 2301 2534 2655 2971 3314 513 Size: 552 1133 103 233 121 316 343 3074 Fragments arranged by size: 3074 1133 552 343 316 233 121 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3113 3902 3919 4072 4257 4702 325 Size: 1255 1533 789 17 153 185 445 1498 Fragments arranged by size: 1533 1498 1255 789 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2449 4121 5833 Size: 274 80 33 51 1443 472 1672 1712 Cuts at: 5833 96 Size: 138 Fragments arranged by size: 1712 1672 1443 472 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2130 2162 2733 2751 2765 2783 2943 2975 5835 Size: 32 571 18 14 18 160 32 2860 Cuts at: 5835 5867 2130 Size: 32 2138 Fragments arranged by size: 2860 2138 571 160 32 32 32 18 18 14 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5875 XbaI T'CTAG_A Cuts at: 3211 3211 Size: 5875 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3158 3158 Size: 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5875 XmaI C'CCGG_G Cuts at: 1870 2017 3200 1870 Size: 147 1183 4545 Fragments arranged by size: 4545 1183 147 XmnI GAAnn'nnTTC Cuts at: 4004 4004 Size: 5875 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3683 276 Size: 53 83 186 936 2149 2468 Fragments arranged by size: 2468 2149 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI