(Linear) (Six-base) MAPSORT of: KIEE6330.seq Check: 4075 from: 1 to: 1170 KIEE6330.orf With 182 enzymes: SgfI * March 16, 2015 11:29 .. AccI GT'mk_AC Cuts at: 0 302 1170 Size: 302 868 Acc65I G'GTAC_C Cuts at: 0 766 1170 Size: 766 404 AgeI A'CCGG_T Cuts at: 0 113 1170 Size: 113 1057 AlwNI CAG_nnn'CTG Cuts at: 0 733 1023 1170 Size: 733 290 147 Fragments arranged by size: 733 290 147 ApaI G_GGCC'C Cuts at: 0 20 87 1059 1170 Size: 20 67 972 111 Fragments arranged by size: 972 111 67 20 AvaI C'yCGr_G Cuts at: 0 12 1170 Size: 12 1158 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 758 791 1170 Size: 758 33 379 Fragments arranged by size: 758 379 33 BanI G'GyrC_C Cuts at: 0 201 766 1137 1170 Size: 201 565 371 33 Fragments arranged by size: 565 371 201 33 BanII G_rGCy'C Cuts at: 0 20 87 901 954 1059 1170 Size: 20 67 814 53 105 111 Fragments arranged by size: 814 111 105 67 53 20 BbsI GAAGACnn'nnnn_ Cuts at: 0 173 638 1170 Size: 173 465 532 Fragments arranged by size: 532 465 173 BciVI GTATCCnnnnn_n' Cuts at: 0 303 1170 Size: 303 867 Bme1580I G_kGCm'C Cuts at: 0 20 66 87 206 491 694 1059 1170 Size: 20 46 21 119 285 203 365 111 Fragments arranged by size: 365 285 203 119 111 46 21 20 BmrI ACTGGGnnnn_n' Cuts at: 0 790 998 1170 Size: 790 208 172 Fragments arranged by size: 790 208 172 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 375 1170 Size: 375 795 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 331 358 440 463 677 755 773 Size: 308 23 27 82 23 214 78 18 Cuts at: 773 853 996 1170 Size: 80 143 174 Fragments arranged by size: 308 214 174 143 82 80 78 27 23 23 18 Bpu10I CC'TnA_GC Cuts at: 0 514 1170 Size: 514 656 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1128 1170 Size: 1128 42 BsaI GGTCTCn'nnnn_ Cuts at: 0 349 1170 Size: 349 821 BsaWI w'CCGG_w Cuts at: 0 113 1170 Size: 113 1057 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 125 155 361 391 650 680 728 746 Size: 125 30 206 30 259 30 48 18 Cuts at: 746 758 776 895 925 938 968 1170 Size: 12 18 119 30 13 30 202 Fragments arranged by size: 259 206 202 125 119 48 30 30 30 30 30 18 18 13 12 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 452 850 1170 Size: 452 398 320 Fragments arranged by size: 452 398 320 BseYI C'CCAG_C Cuts at: 0 86 188 793 1170 Size: 86 102 605 377 Fragments arranged by size: 605 377 102 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 82 1170 Size: 82 1088 BsiHKAI G_wGCw'C Cuts at: 0 727 901 1170 Size: 727 174 269 Fragments arranged by size: 727 269 174 BsmBI CGTCTCn'nnnn_ Cuts at: 0 481 1170 Size: 481 689 Bsp1286I G_dGCh'C Cuts at: 0 20 66 87 206 491 694 727 901 Size: 20 46 21 119 285 203 33 174 Cuts at: 901 954 1059 1170 Size: 53 105 111 Fragments arranged by size: 285 203 174 119 111 105 53 46 33 21 20 BsrDI GCAATG_nn' Cuts at: 0 866 1170 Size: 866 304 BsrFI r'CCGG_y Cuts at: 0 113 1170 Size: 113 1057 BssSI C'ACGA_G Cuts at: 0 947 1170 Size: 947 223 BstAPI GCAn_nnn'nTGC Cuts at: 0 748 1170 Size: 748 422 BstEII G'GTnAC_C Cuts at: 0 24 1170 Size: 24 1146 BtgI C'CryG_G Cuts at: 0 193 615 650 966 1170 Size: 193 422 35 316 204 Fragments arranged by size: 422 316 204 193 35 BtsI GCAGTG_nn' Cuts at: 0 692 749 1170 Size: 692 57 421 Fragments arranged by size: 692 421 57 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 538 573 1170 Size: 538 35 597 Fragments arranged by size: 597 538 35 EaeI y'GGCC_r Cuts at: 0 958 1170 Size: 958 212 EcoICRI GAG'CTC Cuts at: 0 899 1170 Size: 899 271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 308 331 358 440 463 677 755 773 Size: 308 23 27 82 23 214 78 18 Cuts at: 773 853 996 1170 Size: 80 143 174 Fragments arranged by size: 308 214 174 143 82 80 78 27 23 23 18 EcoO109I rG'GnC_Cy Cuts at: 0 16 17 730 828 923 1020 1055 1170 Size: 16 1 713 98 95 97 35 115 Fragments arranged by size: 713 115 98 97 95 35 16 1 EcoRV GAT'ATC Cuts at: 0 852 1170 Size: 852 318 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 163 1170 Size: 163 1007 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 650 682 895 927 1170 Size: 650 32 213 32 243 Fragments arranged by size: 650 243 213 32 32 KpnI G_GTAC'C Cuts at: 0 770 1170 Size: 770 400 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 756 1170 Size: 756 414 MspA1I CmG'CkG Cuts at: 0 90 374 544 1170 Size: 90 284 170 626 Fragments arranged by size: 626 284 170 90 NcoI C'CATG_G Cuts at: 0 193 650 966 1170 Size: 193 457 316 204 Fragments arranged by size: 457 316 204 193 PflMI CCAn_nnn'nTGG Cuts at: 0 967 1170 Size: 967 203 PfoI T'CCnGG_A Cuts at: 0 316 347 1170 Size: 316 31 823 Fragments arranged by size: 823 316 31 PpuMI rG'GwC_Cy Cuts at: 0 923 1170 Size: 923 247 PspOMI G'GGCC_C Cuts at: 0 16 83 1055 1170 Size: 16 67 972 115 Fragments arranged by size: 972 115 67 16 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 428 460 1170 Size: 428 32 710 Fragments arranged by size: 710 428 32 PstI C_TGCA'G Cuts at: 0 744 1170 Size: 744 426 PvuII CAG'CTG Cuts at: 0 90 374 1170 Size: 90 284 796 Fragments arranged by size: 796 284 90 SacI G_AGCT'C Cuts at: 0 901 1170 Size: 901 269 SanDI GG'GwC_CC Cuts at: 0 923 1170 Size: 923 247 SfcI C'TryA_G Cuts at: 0 369 740 1170 Size: 369 371 430 Fragments arranged by size: 430 371 369 SmaI CCC'GGG Cuts at: 0 14 1170 Size: 14 1156 SmlI C'TyrA_G Cuts at: 0 1107 1170 Size: 1107 63 StuI AGG'CCT Cuts at: 0 981 1170 Size: 981 189 StyI C'CwwG_G Cuts at: 0 193 296 529 650 966 1170 Size: 193 103 233 121 316 204 Fragments arranged by size: 316 233 204 193 121 103 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1108 1170 Size: 1108 62 TatI w'GTAC_w Cuts at: 0 444 1170 Size: 444 726 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 125 157 728 746 760 778 938 970 Size: 125 32 571 18 14 18 160 32 Cuts at: 970 1170 Size: 200 Fragments arranged by size: 571 200 160 125 32 32 18 18 14 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1153 1170 Size: 1153 17 XmaI C'CCGG_G Cuts at: 0 12 1170 Size: 12 1158 Enzymes that do cut: AccI Acc65I AgeI AlwNI ApaI AvaI BaeI BanI BanII BbsI BciVI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BsrDI BsrFI BssSI BstAPI BstEII BtgI BtsI CspCI EaeI EcoICRI Eco57MI EcoO109I EcoRV FalI Hin4I KpnI MmeI MspA1I NcoI PflMI PfoI PpuMI PspOMI PsrI PstI PvuII SacI SanDI SfcI SmaI SmlI StuI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII AclI AcuI AfeI AflII AflIII AhdI AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI BclI BfrBI BglI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaHI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI BsrGI BssHII BstBI BstXI BstYI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI FseI FspI FspAI HaeII HincII HindIII HpaI KasI MfeI MluI MscI MslI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI Tth111I XbaI XhoI XmnI ZraI