Restriction map for pFN21AE6330
 (Linear) (Six-base) MAPSORT of: KIEE6330.seq  Check: 4075  from: 1  to: 1170



KIEE6330.orf

 With 182 enzymes: SgfI * 

                        March 16, 2015 11:29  ..

AccI GT'mk_AC

Cuts at:      0     302    1170 
   Size:        302     868
 
Acc65I G'GTAC_C

Cuts at:      0     766    1170 
   Size:        766     404
 
AgeI A'CCGG_T

Cuts at:      0     113    1170 
   Size:        113    1057
 
AlwNI CAG_nnn'CTG

Cuts at:      0     733    1023    1170 
   Size:        733     290     147

  Fragments arranged by size: 

                 733     290     147
 
ApaI G_GGCC'C

Cuts at:      0      20      87    1059    1170 
   Size:         20      67     972     111

  Fragments arranged by size: 

                 972     111      67      20
 
AvaI C'yCGr_G

Cuts at:      0      12    1170 
   Size:         12    1158
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     758     791    1170 
   Size:        758      33     379

  Fragments arranged by size: 

                 758     379      33
 
BanI G'GyrC_C

Cuts at:      0     201     766    1137    1170 
   Size:        201     565     371      33

  Fragments arranged by size: 

                 565     371     201      33
 
BanII G_rGCy'C

Cuts at:      0      20      87     901     954    1059    1170 
   Size:         20      67     814      53     105     111

  Fragments arranged by size: 

                 814     111     105      67      53      20
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     173     638    1170 
   Size:        173     465     532

  Fragments arranged by size: 

                 532     465     173
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     303    1170 
   Size:        303     867
 
Bme1580I G_kGCm'C

Cuts at:      0      20      66      87     206     491     694    1059    1170 
   Size:         20      46      21     119     285     203     365     111

  Fragments arranged by size: 

                 365     285     203     119     111      46      21      20
            
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     790     998    1170 
   Size:        790     208     172

  Fragments arranged by size: 

                 790     208     172
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     375    1170 
   Size:        375     795
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     331     358     440     463     677     755     773 
   Size:        308      23      27      82      23     214      78      18

Cuts at:    773     853     996    1170 
   Size:         80     143     174

  Fragments arranged by size: 

                 308     214     174     143      82      80      78      27
                  23      23      18
 
Bpu10I CC'TnA_GC

Cuts at:      0     514    1170 
   Size:        514     656
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1128    1170 
   Size:       1128      42
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     349    1170 
   Size:        349     821
 
BsaWI w'CCGG_w

Cuts at:      0     113    1170 
   Size:        113    1057
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     125     155     361     391     650     680     728     746 
   Size:        125      30     206      30     259      30      48      18

Cuts at:    746     758     776     895     925     938     968    1170 
   Size:         12      18     119      30      13      30     202

  Fragments arranged by size: 

                 259     206     202     125     119      48      30      30
                  30      30      30      18      18      13      12
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     452     850    1170 
   Size:        452     398     320

  Fragments arranged by size: 

                 452     398     320
 
BseYI C'CCAG_C

Cuts at:      0      86     188     793    1170 
   Size:         86     102     605     377

  Fragments arranged by size: 

                 605     377     102      86
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      82    1170 
   Size:         82    1088
 
BsiHKAI G_wGCw'C

Cuts at:      0     727     901    1170 
   Size:        727     174     269

  Fragments arranged by size: 

                 727     269     174
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     481    1170 
   Size:        481     689
 
Bsp1286I G_dGCh'C

Cuts at:      0      20      66      87     206     491     694     727     901 
   Size:         20      46      21     119     285     203      33     174

Cuts at:    901     954    1059    1170 
   Size:         53     105     111

  Fragments arranged by size: 

                 285     203     174     119     111     105      53      46
                  33      21      20
 
BsrDI GCAATG_nn'

Cuts at:      0     866    1170 
   Size:        866     304
 
BsrFI r'CCGG_y

Cuts at:      0     113    1170 
   Size:        113    1057
 
BssSI C'ACGA_G

Cuts at:      0     947    1170 
   Size:        947     223
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     748    1170 
   Size:        748     422
 
BstEII G'GTnAC_C

Cuts at:      0      24    1170 
   Size:         24    1146
 
BtgI C'CryG_G

Cuts at:      0     193     615     650     966    1170 
   Size:        193     422      35     316     204

  Fragments arranged by size: 

                 422     316     204     193      35
 
BtsI GCAGTG_nn'

Cuts at:      0     692     749    1170 
   Size:        692      57     421

  Fragments arranged by size: 

                 692     421      57
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     538     573    1170 
   Size:        538      35     597

  Fragments arranged by size: 

                 597     538      35
 
EaeI y'GGCC_r

Cuts at:      0     958    1170 
   Size:        958     212
 
EcoICRI GAG'CTC

Cuts at:      0     899    1170 
   Size:        899     271
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     331     358     440     463     677     755     773 
   Size:        308      23      27      82      23     214      78      18

Cuts at:    773     853     996    1170 
   Size:         80     143     174

  Fragments arranged by size: 

                 308     214     174     143      82      80      78      27
                  23      23      18
 
EcoO109I rG'GnC_Cy

Cuts at:      0      16      17     730     828     923    1020    1055    1170 
   Size:         16       1     713      98      95      97      35     115

  Fragments arranged by size: 

                 713     115      98      97      95      35      16       1
            
 
EcoRV GAT'ATC

Cuts at:      0     852    1170 
   Size:        852     318
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     163    1170 
   Size:        163    1007
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     650     682     895     927    1170 
   Size:        650      32     213      32     243

  Fragments arranged by size: 

                 650     243     213      32      32
 
KpnI G_GTAC'C

Cuts at:      0     770    1170 
   Size:        770     400
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     756    1170 
   Size:        756     414
 
MspA1I CmG'CkG

Cuts at:      0      90     374     544    1170 
   Size:         90     284     170     626

  Fragments arranged by size: 

                 626     284     170      90
 
NcoI C'CATG_G

Cuts at:      0     193     650     966    1170 
   Size:        193     457     316     204

  Fragments arranged by size: 

                 457     316     204     193
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     967    1170 
   Size:        967     203
 
PfoI T'CCnGG_A

Cuts at:      0     316     347    1170 
   Size:        316      31     823

  Fragments arranged by size: 

                 823     316      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     923    1170 
   Size:        923     247
 
PspOMI G'GGCC_C

Cuts at:      0      16      83    1055    1170 
   Size:         16      67     972     115

  Fragments arranged by size: 

                 972     115      67      16
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     428     460    1170 
   Size:        428      32     710

  Fragments arranged by size: 

                 710     428      32
 
PstI C_TGCA'G

Cuts at:      0     744    1170 
   Size:        744     426
 
PvuII CAG'CTG

Cuts at:      0      90     374    1170 
   Size:         90     284     796

  Fragments arranged by size: 

                 796     284      90
 
SacI G_AGCT'C

Cuts at:      0     901    1170 
   Size:        901     269
 
SanDI GG'GwC_CC

Cuts at:      0     923    1170 
   Size:        923     247
 
SfcI C'TryA_G

Cuts at:      0     369     740    1170 
   Size:        369     371     430

  Fragments arranged by size: 

                 430     371     369
 
SmaI CCC'GGG

Cuts at:      0      14    1170 
   Size:         14    1156
 
SmlI C'TyrA_G

Cuts at:      0    1107    1170 
   Size:       1107      63
 
StuI AGG'CCT

Cuts at:      0     981    1170 
   Size:        981     189
 
StyI C'CwwG_G

Cuts at:      0     193     296     529     650     966    1170 
   Size:        193     103     233     121     316     204

  Fragments arranged by size: 

                 316     233     204     193     121     103
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1108    1170 
   Size:       1108      62
 
TatI w'GTAC_w

Cuts at:      0     444    1170 
   Size:        444     726
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     125     157     728     746     760     778     938     970 
   Size:        125      32     571      18      14      18     160      32

Cuts at:    970    1170 
   Size:        200

  Fragments arranged by size: 

                 571     200     160     125      32      32      18      18
                  14
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1153    1170 
   Size:       1153      17
 
XmaI C'CCGG_G

Cuts at:      0      12    1170 
   Size:         12    1158
 

 Enzymes that do cut:

     AccI   Acc65I     AgeI    AlwNI     ApaI     AvaI     BaeI     BanI
    BanII     BbsI    BciVI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaWI    BsaXI    BseRI    BseYI     BsgI  BsiHKAI
    BsmBI Bsp1286I    BsrDI    BsrFI    BssSI   BstAPI   BstEII     BtgI
     BtsI    CspCI     EaeI  EcoICRI  Eco57MI EcoO109I    EcoRV     FalI
    Hin4I     KpnI     MmeI   MspA1I     NcoI    PflMI     PfoI    PpuMI
   PspOMI     PsrI     PstI    PvuII     SacI    SanDI     SfcI     SmaI
     SmlI     StuI     StyI    TaqII     TatI     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AcuI     AfeI    AflII   AflIII     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII    BamHI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI    BsaAI    BsaBI    BsaHI    BsiEI
    BsiWI     BsmI    BspEI    BspHI    BspMI    BsrBI    BsrGI   BssHII
    BstBI    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI     DraI
   DraIII     DrdI     EagI     EarI     EciI    EcoNI    EcoRI     FseI
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     MfeI
     MluI     MscI     MslI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PshAI     PsiI    PspXI     PvuI    RsrII    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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