################################## # NO CUT ENZYMES ( 170 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AlwNI AocI Aor51HI ApaI ApoI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I CelII ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MseI MslI MunI NarI NciI NcoI NdeI NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacII SalI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AfaI 171 GTAC GT^AC Alw44I 91 GTGCAC G^TGCAC AlwI 186 GATCC GGATC(4/5) ApaLI 91 GTGCAC G^TGCAC AscI 139 GGCGCGCC GG^CGCGCC BalI 257 TGGCCA TGG^CCA BanII 277 GAGCTC G[GA]GC[CT]^C BbsI 225 GAAGAC GAAGAC(2/6) BfaI 59 CTAG C^TAG BpuAI 225 GAAGAC GAAGAC(2/6) BsaI 67 GGTCTC GGTCTC(1/5) BsaJI 80 CCGTGG C^C[ACGT][ACGT]GG BseRI 274 GAGGAG GAGGAG(10/8) BsmAI 68 GTCTC GTCTC(1/5) BsrBI 124 GAGCGG CCGCTC(-3/-3) BssHII 140 GCGCGC G^CGCGC BssKI 189 CCTGG ^CC[ACGT]GG BstAPI 93 GCACCGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 189 CCTGG CC^[AT]GG BstYI 185 AGATCC [GA]^GATC[CT] DpnII 186 GATC ^GATC DsaI 80 CCGTGG C^C[GA][CT]GG EaeI 257 TGGCCA [CT]^GGCC[GA] EarI 54 CTCTTC CTCTTC(1/4) EcoRII 189 CCTGG ^CC[AT]GG HgaI 243 GCGTC GACGC(5/10) HinfI 48 GACTC G^A[ACGT]TC HphI 302 TCACC GGTGA(8/7) Ksp632I 54 CTCTTC CTCTTC(1/4) MaeI 59 CTAG C^TAG MaeII 9 ACGT A^CGT MaeIII 301 GTCAC ^GT[ACGT]AC MboI 186 GATC ^GATC MflI 185 AGATCC [GA]^GATC[CT] MluNI 257 TGGCCA TGG^CCA MscI 257 TGGCCA TGG^CCA MspA1I 158 CAGCGG C[AC]G^C[GT]G MvaI 189 CCTGG CC^[AT]GG NaeI 136 GCCGGC GCC^GGC NdeII 186 GATC ^GATC NgoMIV 136 GCCGGC G^CCGGC NlaIV 133 GGGGCC GG[ACGT]^[ACGT]CC PleI 48 GACTC GAGTC(4/5) RsaI 171 GTAC GT^AC SacI 277 GAGCTC GAGCT^C SapI 53 GCTCTTC GCTCTTC(1/4) Sau3AI 186 GATC ^GATC ScrFI 189 CCTGG CC^[ACGT]GG SfaNI 233 GCATC GCATC(5/9) SgrAI 143 CGCCGGTG C[GA]^CCGG[CT]G Tsp45I 301 GTCAC ^GT[GC]AC XhoII 185 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AluI 278 AGCT AG^CT 287 AGCT AspHI 91 GTGCAC G[AT]GC[AT]^C 277 GAGCTC BbvI 157 GCAGC GCAGC(8/12) 288 GCTGC BmyI 91 GTGCAC G[AGT]GC[ACT]^C 277 GAGCTC BpmI 70 CTCCAG CTGGAG(16/14) 166 CTGGAG BsiHKAI 91 GTGCAC G[AT]GC[AT]^C 277 GAGCTC Bsp1286I 91 GTGCAC G[AGT]GC[ACT]^C 277 GAGCTC BstF5I 237 CATCC GGATG(2/0) 261 CATCC DdeI 38 CTAAG C^T[ACGT]AG 362 CTGAG FokI 237 CATCC GGATG(9/13) 261 CATCC MboII 225 GAAGA GAAGA(8/7) 55 TCTTC MnlI 23 GAGG CCTC(7/6) 274 GAGG TseI 157 GCAGC G^C[AT]GC 288 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 23 enzymes) # ######################################### AccII AciI BsiYI BslI BsrFI BsrI BstUI Cac8I CfoI Cfr10I Cfr13I FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MspI MvnI MwoI Sau96I