(Circular) (Six-base) MAPSORT of: pFN21AE1384.seq Check: 6906 from: 1 to: 6499 pFN21AE1384 6499 bp With 182 enzymes: SgfI * August 16, 2010 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4309 278 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3842 1937 Size: 1905 4594 Acc65I G'GTAC_C Cuts at: 3820 3820 Size: 6499 AclI AA'CG_TT Cuts at: 1563 4626 4999 1563 Size: 3063 373 3063 Fragments arranged by size: 3063 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2146 2563 2645 3050 3629 4562 5946 6214 Size: 514 417 82 405 579 933 1384 268 Cuts at: 6214 1632 Size: 1917 Fragments arranged by size: 1917 1384 933 579 514 417 405 268 82 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6302 AflIII A'CryG_T Cuts at: 1203 2852 5398 6375 1203 Size: 1649 2546 977 1327 Fragments arranged by size: 2546 1649 1327 977 AgeI A'CCGG_T Cuts at: 5330 5330 Size: 6499 AhdI GACnn_n'nnGTC Cuts at: 5228 5228 Size: 6499 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6499 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6499 AlwNI CAG_nnn'CTG Cuts at: 2409 3172 3313 3712 4242 5814 2409 Size: 763 141 399 530 1572 3094 Fragments arranged by size: 3094 1572 763 530 399 141 ApaI G_GGCC'C Cuts at: 2553 2782 3135 2553 Size: 229 353 5917 Fragments arranged by size: 5917 353 229 ApaLI G'TGCA_C Cuts at: 3114 3700 4556 5712 3114 Size: 586 856 1156 3901 Fragments arranged by size: 3901 1156 856 586 ApoI r'AATT_y Cuts at: 1493 3808 4034 4186 6295 1493 Size: 2315 226 152 2109 1697 Fragments arranged by size: 2315 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5053 160 Size: 872 4021 1606 Fragments arranged by size: 4021 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 AvaI C'yCGr_G Cuts at: 1870 1943 2123 2731 3683 3824 1870 Size: 73 180 608 952 141 4545 Fragments arranged by size: 4545 952 608 180 141 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2736 2769 2736 Size: 33 6466 BamHI G'GATC_C Cuts at: 3829 4196 3829 Size: 367 6132 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2704 3748 3820 5275 618 Size: 325 217 627 917 1044 72 1455 1842 Fragments arranged by size: 1842 1455 1044 917 627 325 217 72 BanII G_rGCy'C Cuts at: 1391 2553 2621 2732 2782 3135 3342 3642 3818 Size: 1162 68 111 50 353 207 300 176 Cuts at: 3818 1391 Size: 4072 Fragments arranged by size: 4072 1162 353 300 207 176 111 68 50 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5528 BbvCI CC'TCA_GC Cuts at: 2242 2278 2242 Size: 36 6463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2940 2974 3488 3522 4690 Size: 34 50 34 919 34 514 34 1168 Cuts at: 4690 4724 1903 Size: 34 3678 Fragments arranged by size: 3678 1168 919 514 50 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2024 2730 2737 3470 4391 5601 2024 Size: 706 7 733 921 1210 2922 Fragments arranged by size: 2922 1210 921 733 706 7 BclI T'GATC_A Cuts at: 1548 1548 Size: 6499 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5110 136 Size: 107 122 71 631 605 3438 1525 Fragments arranged by size: 3438 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3774 6494 3774 Size: 2720 3779 BlpI GC'TnA_GC Cuts at: 3288 3916 3288 Size: 628 5871 Bme1580I G_kGCm'C Cuts at: 1790 2553 2782 3118 3135 3433 3704 3753 4560 Size: 763 229 336 17 298 271 49 807 Cuts at: 4560 5716 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 807 763 336 298 271 229 49 17 BmgBI CAC'GTC Cuts at: 1318 3665 1318 Size: 2347 4152 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2354 4230 5188 Size: 416 53 83 399 24 937 1876 958 Cuts at: 5188 442 Size: 1753 Fragments arranged by size: 1876 1753 958 937 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6499 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2365 2547 3266 3341 3356 3758 5159 1239 Size: 1126 182 719 75 15 402 1401 2579 Fragments arranged by size: 2579 1401 1126 719 402 182 75 15 Bpu10I CC'TnA_GC Cuts at: 2242 2278 2351 3400 2242 Size: 36 73 1049 5341 Fragments arranged by size: 5341 1049 73 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2744 3457 3716 4624 5489 5787 2744 Size: 713 259 908 865 298 3456 Fragments arranged by size: 3456 908 865 713 298 259 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5162 882 Size: 617 3663 2219 Fragments arranged by size: 3663 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3619 4290 493 Size: 711 2415 671 2702 Fragments arranged by size: 2702 2415 711 671 BsaBI GATnn'nnATC Cuts at: 6493 6493 Size: 6499 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4306 4688 5323 275 Size: 53 83 186 936 2773 382 635 1451 Fragments arranged by size: 2773 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3236 4932 5330 5604 5751 6162 3236 Size: 1696 398 274 147 411 3573 Fragments arranged by size: 3573 1696 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3218 3248 1178 Size: 30 165 30 1815 30 4429 Fragments arranged by size: 4429 1815 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2230 2239 2242 2251 3086 3593 1186 Size: 1044 9 3 9 835 507 4092 Fragments arranged by size: 4092 1044 835 507 9 9 3 BseYI C'CCAG_C Cuts at: 2156 2435 2451 2492 2597 2603 2656 3134 3203 Size: 279 16 41 105 6 53 478 69 Cuts at: 3203 3642 5702 6198 2156 Size: 439 2060 496 2457 Fragments arranged by size: 2457 2060 496 478 439 279 105 69 53 41 16 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 3467 1163 Size: 573 1173 558 4195 Fragments arranged by size: 4195 1173 573 558 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 3949 4710 5738 1540 Size: 275 186 1546 402 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 402 275 186 BsiHKAI G_wGCw'C Cuts at: 3118 3342 3618 3704 4560 4645 5716 3118 Size: 224 276 86 856 85 1071 3901 Fragments arranged by size: 3901 1071 856 276 224 86 85 BsmI GAATG_Cn' Cuts at: 4010 4103 4010 Size: 93 6406 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 2621 2732 2782 3118 3135 3342 Size: 399 763 68 111 50 336 17 207 Cuts at: 3342 3433 3618 3642 3704 3753 3818 4560 4645 Size: 91 185 24 62 49 65 742 85 Cuts at: 4645 5716 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 763 742 399 336 207 185 111 91 85 68 65 62 50 49 24 17 BspEI T'CCGG_A Cuts at: 6162 6162 Size: 6499 BspHI T'CATG_A Cuts at: 4388 6118 4388 Size: 1730 4769 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3237 3854 844 Size: 2393 617 3489 Fragments arranged by size: 3489 2393 617 BsrBI CCG'CTC Cuts at: 1394 1823 2355 2775 4386 1394 Size: 429 532 420 1611 3507 Fragments arranged by size: 3507 1611 532 429 420 BsrDI GCAATG_nn' Cuts at: 65 1248 4994 5168 65 Size: 1183 3746 174 1396 Fragments arranged by size: 3746 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2591 3045 3298 3417 3546 5143 5330 Size: 110 781 454 253 119 129 1597 187 Cuts at: 5330 1700 Size: 2869 Fragments arranged by size: 2869 1597 781 454 253 187 129 119 110 BsrGI T'GTAC_A Cuts at: 96 3211 96 Size: 3115 3384 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6499 BssSI C'ACGA_G Cuts at: 4559 5571 4559 Size: 1012 5487 BstAPI GCAn_nnn'nTGC Cuts at: 794 2409 3490 3556 794 Size: 1615 1081 66 3737 Fragments arranged by size: 3737 1615 1081 66 BstBI TT'CG_AA Cuts at: 5303 5303 Size: 6499 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6499 BstXI CCAn_nnnn'nTGG Cuts at: 2113 2840 2113 Size: 727 5772 BstYI r'GATC_y Cuts at: 1972 3273 3774 3829 4196 4581 4598 6050 6136 Size: 1301 501 55 367 385 17 1452 86 Cuts at: 6136 6148 6494 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1301 501 385 367 346 86 55 17 12 BtgI C'CryG_G Cuts at: 513 1065 2106 3433 3566 513 Size: 552 1041 1327 133 3446 Fragments arranged by size: 3446 1327 1041 552 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3580 487 Size: 683 56 2354 3406 Fragments arranged by size: 3406 2354 683 56 BtsI GCAGTG_nn' Cuts at: 2145 3138 3431 4015 4809 4829 2145 Size: 993 293 584 794 20 3815 Fragments arranged by size: 3815 993 794 584 293 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6499 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6464 DraI TTT'AAA Cuts at: 3803 3803 Size: 6499 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5506 809 Size: 507 4190 1802 Fragments arranged by size: 4190 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2479 3946 Size: 54 1425 50 275 18 285 364 1467 Cuts at: 3946 4835 6464 8 Size: 889 1629 43 Fragments arranged by size: 1629 1467 1425 889 364 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3946 1537 Size: 275 2134 4090 Fragments arranged by size: 4090 2134 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3347 4429 1353 Size: 1994 1082 3423 Fragments arranged by size: 3423 1994 1082 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3281 3341 5086 5460 5606 387 Size: 1064 138 1692 60 1745 374 146 1280 Fragments arranged by size: 1745 1692 1280 1064 374 146 138 60 EcoICRI GAG'CTC Cuts at: 3340 3340 Size: 6499 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2146 2365 2547 2563 2645 3050 3266 Size: 393 514 219 182 16 82 405 216 Cuts at: 3266 3341 3356 3629 3758 4562 5159 5946 6214 Size: 75 15 273 129 804 597 787 268 Cuts at: 6214 1239 Size: 1524 Fragments arranged by size: 1524 804 787 597 514 405 393 273 268 219 216 182 129 82 75 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 3472 3472 Size: 6499 EcoO109I rG'GnC_Cy Cuts at: 2051 2371 2550 2778 2779 3131 3310 3477 3709 Size: 320 179 228 1 352 179 167 232 Cuts at: 3709 2051 Size: 4841 Fragments arranged by size: 4841 352 320 232 228 179 179 167 1 EcoRI G'AATT_C Cuts at: 3808 4186 3808 Size: 378 6121 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 3409 2890 Size: 519 5980 FspI TGC'GCA Cuts at: 5005 5005 Size: 6499 HaeII r_GCGC'y Cuts at: 1678 5646 1678 Size: 3968 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2360 2392 3218 3250 5140 Size: 32 135 32 788 32 826 32 1890 Cuts at: 5140 5172 5214 5246 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1890 826 788 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3843 4089 669 Size: 988 281 1905 246 3079 Fragments arranged by size: 3079 1905 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6499 HpaI GTT'AAC Cuts at: 4089 4089 Size: 6499 KpnI G_GTAC'C Cuts at: 3824 3824 Size: 6499 MfeI C'AATT_G Cuts at: 4098 4098 Size: 6499 MluI A'CGCG_T Cuts at: 6375 6375 Size: 6499 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5613 5797 1305 Size: 4308 184 2007 Fragments arranged by size: 4308 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 4392 Fragments arranged by size: 4392 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2841 4457 4816 4975 518 Size: 648 1005 670 1616 359 159 2042 Fragments arranged by size: 2042 1616 1005 670 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2138 2215 2533 2656 2802 2958 3568 3603 Size: 532 77 318 123 146 156 610 35 Cuts at: 3603 3626 3654 3915 4592 5740 5985 1606 Size: 23 28 261 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 610 532 318 261 245 156 146 123 77 35 28 23 NaeI GCC'GGC Cuts at: 3047 3047 Size: 6499 NcoI C'CATG_G Cuts at: 513 1065 2106 513 Size: 552 1041 4906 Fragments arranged by size: 4906 1041 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6499 NgoMIV G'CCGG_C Cuts at: 3045 3045 Size: 6499 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6499 NotI GC'GGCC_GC Cuts at: 3946 3946 Size: 6499 NspI r_CATG'y Cuts at: 1132 1229 2856 3857 5402 1132 Size: 97 1627 1001 1545 2229 Fragments arranged by size: 2229 1627 1545 1001 97 PasI CC'CwG_GG Cuts at: 1348 2463 3706 1348 Size: 1115 1243 4141 Fragments arranged by size: 4141 1243 1115 PciI A'CATG_T Cuts at: 2852 5398 2852 Size: 2546 3953 PflMI CCAn_nnn'nTGG Cuts at: 1108 2656 3172 1108 Size: 1548 516 4435 Fragments arranged by size: 4435 1548 516 PfoI T'CCnGG_A Cuts at: 2047 3165 3333 2047 Size: 1118 168 5213 Fragments arranged by size: 5213 1118 168 PmeI GTTT'AAAC Cuts at: 3803 3803 Size: 6499 PmlI CAC'GTG Cuts at: 3619 3619 Size: 6499 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4568 4600 6081 6113 4568 Size: 32 1481 32 4954 Fragments arranged by size: 4954 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2051 3310 3477 2051 Size: 1259 167 5073 Fragments arranged by size: 5073 1259 167 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6499 PsiI TTA'TAA Cuts at: 4069 4069 Size: 6499 PspOMI G'GGCC_C Cuts at: 2549 2778 3131 2549 Size: 229 353 5917 Fragments arranged by size: 5917 353 229 PstI C_TGCA'G Cuts at: 830 3426 3561 3588 3717 3851 830 Size: 2596 135 27 129 134 3478 Fragments arranged by size: 3478 2596 135 134 129 27 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6499 PvuII CAG'CTG Cuts at: 2215 2533 2656 3603 3626 3654 2215 Size: 318 123 947 23 28 5060 Fragments arranged by size: 5060 947 318 123 28 23 SacI G_AGCT'C Cuts at: 3342 3342 Size: 6499 SacII CC_GC'GG Cuts at: 3569 3569 Size: 6499 SalI G'TCGA_C Cuts at: 1936 3841 1936 Size: 1905 4594 SanDI GG'GwC_CC Cuts at: 2051 2051 Size: 6499 SapI GCTCTTCn'nnn_ Cuts at: 3347 3347 Size: 6499 SbfI CC_TGCA'GG Cuts at: 3851 3851 Size: 6499 ScaI AGT'ACT Cuts at: 4747 6459 4747 Size: 1712 4787 SfcI C'TryA_G Cuts at: 826 1045 3422 3557 3584 3713 3847 4982 5663 Size: 219 2377 135 27 129 134 1135 681 Cuts at: 5663 5854 826 Size: 191 1471 Fragments arranged by size: 2377 1471 1135 681 219 191 135 134 129 27 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6499 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6499 SmaI CCC'GGG Cuts at: 1872 3826 1872 Size: 1954 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2723 3472 3731 4603 5504 5766 Size: 197 926 780 749 259 872 901 262 Cuts at: 5766 820 Size: 1553 Fragments arranged by size: 1553 926 901 872 780 749 262 259 197 SnaBI TAC'GTA Cuts at: 493 4290 493 Size: 3797 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6499 SphI G_CATG'C Cuts at: 1132 3857 1132 Size: 2725 3774 SspI AAT'ATT Cuts at: 5 52 4423 5 Size: 47 4371 2081 Fragments arranged by size: 4371 2081 47 StuI AGG'CCT Cuts at: 1517 2511 3405 1517 Size: 994 894 4611 Fragments arranged by size: 4611 994 894 StyI C'CwwG_G Cuts at: 513 1065 2106 2763 3938 513 Size: 552 1041 657 1175 3074 Fragments arranged by size: 3074 1175 1041 657 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2663 2722 3719 3775 4526 4543 4696 Size: 1255 1083 59 997 56 751 17 153 Cuts at: 4696 4881 5326 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 1083 997 751 445 185 153 59 56 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2904 3211 4745 Size: 274 80 33 51 1443 927 307 1534 Cuts at: 4745 6457 96 Size: 1712 138 Fragments arranged by size: 1712 1534 1443 927 307 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6459 6491 6459 Size: 32 6467 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6499 XbaI T'CTAG_A Cuts at: 3835 3835 Size: 6499 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2024 2110 2605 2653 2024 Size: 86 495 48 5870 Fragments arranged by size: 5870 495 86 48 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6499 XmaI C'CCGG_G Cuts at: 1870 3824 1870 Size: 1954 4545 XmnI GAAnn'nnTTC Cuts at: 2630 4628 2630 Size: 1998 4501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4307 276 Size: 53 83 186 936 2773 2468 Fragments arranged by size: 2773 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SexAI SfiI SfoI SrfI SwaI