(Linear) (Six-base) MAPSORT of: KIEE1384.seq Check: 1550 from: 1 to: 1794 KIEE1384.orf With 182 enzymes: SgfI * July 15, 2010 18:37 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 558 640 1045 1624 1794 Size: 141 417 82 405 579 170 Fragments arranged by size: 579 417 405 170 141 82 AflIII A'CryG_T Cuts at: 0 847 1794 Size: 847 947 AlwNI CAG_nnn'CTG Cuts at: 0 404 1167 1308 1707 1794 Size: 404 763 141 399 87 Fragments arranged by size: 763 404 399 141 87 ApaI G_GGCC'C Cuts at: 0 548 777 1130 1794 Size: 548 229 353 664 Fragments arranged by size: 664 548 353 229 ApaLI G'TGCA_C Cuts at: 0 1109 1695 1794 Size: 1109 586 99 Fragments arranged by size: 1109 586 99 AvaI C'yCGr_G Cuts at: 0 118 726 1678 1794 Size: 118 608 952 116 Fragments arranged by size: 952 608 118 116 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 731 764 1794 Size: 731 33 1030 Fragments arranged by size: 1030 731 33 BanI G'GyrC_C Cuts at: 0 699 1743 1794 Size: 699 1044 51 Fragments arranged by size: 1044 699 51 BanII G_rGCy'C Cuts at: 0 548 616 727 777 1130 1337 1637 1794 Size: 548 68 111 50 353 207 300 157 Fragments arranged by size: 548 353 300 207 157 111 68 50 BbvCI CC'TCA_GC Cuts at: 0 237 273 1794 Size: 237 36 1521 Fragments arranged by size: 1521 237 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 935 969 1483 1517 1794 Size: 935 34 514 34 277 Fragments arranged by size: 935 514 277 34 34 BciVI GTATCCnnnnn_n' Cuts at: 0 19 725 732 1465 1794 Size: 19 706 7 733 329 Fragments arranged by size: 733 706 329 19 7 BglII A'GATC_T Cuts at: 0 1769 1794 Size: 1769 25 BlpI GC'TnA_GC Cuts at: 0 1283 1794 Size: 1283 511 Bme1580I G_kGCm'C Cuts at: 0 548 777 1113 1130 1428 1699 1748 1794 Size: 548 229 336 17 298 271 49 46 Fragments arranged by size: 548 336 298 271 229 49 46 17 BmgBI CAC'GTC Cuts at: 0 1660 1794 Size: 1660 134 BmrI ACTGGGnnnn_n' Cuts at: 0 349 1794 Size: 349 1445 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 360 542 1261 1336 1351 1753 1794 Size: 360 182 719 75 15 402 41 Fragments arranged by size: 719 402 360 182 75 41 15 Bpu10I CC'TnA_GC Cuts at: 0 237 273 346 1395 1794 Size: 237 36 73 1049 399 Fragments arranged by size: 1049 399 237 73 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 739 1452 1711 1794 Size: 739 713 259 83 Fragments arranged by size: 739 713 259 83 BsaAI yAC'GTr Cuts at: 0 1614 1794 Size: 1614 180 BsaWI w'CCGG_w Cuts at: 0 1231 1794 Size: 1231 563 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1213 1243 1794 Size: 1213 30 551 Fragments arranged by size: 1213 551 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 225 234 237 246 1081 1588 1794 Size: 225 9 3 9 835 507 206 Fragments arranged by size: 835 507 225 206 9 9 3 BseYI C'CCAG_C Cuts at: 0 151 430 446 487 592 598 651 1129 Size: 151 279 16 41 105 6 53 478 Cuts at: 1129 1198 1637 1794 Size: 69 439 157 Fragments arranged by size: 478 439 279 157 151 105 69 53 41 16 6 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 904 1462 1794 Size: 904 558 332 Fragments arranged by size: 904 558 332 BsiEI CG_ry'CG Cuts at: 0 1542 1794 Size: 1542 252 BsiHKAI G_wGCw'C Cuts at: 0 1113 1337 1613 1699 1794 Size: 1113 224 276 86 95 Fragments arranged by size: 1113 276 224 95 86 Bsp1286I G_dGCh'C Cuts at: 0 548 616 727 777 1113 1130 1337 1428 Size: 548 68 111 50 336 17 207 91 Cuts at: 1428 1613 1637 1699 1748 1794 Size: 185 24 62 49 46 Fragments arranged by size: 548 336 207 185 111 91 68 62 50 49 46 24 17 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1232 1794 Size: 1232 562 BsrBI CCG'CTC Cuts at: 0 350 770 1794 Size: 350 420 1024 Fragments arranged by size: 1024 420 350 BsrFI r'CCGG_y Cuts at: 0 586 1040 1293 1412 1541 1794 Size: 586 454 253 119 129 253 Fragments arranged by size: 586 454 253 253 129 119 BsrGI T'GTAC_A Cuts at: 0 1206 1794 Size: 1206 588 BstAPI GCAn_nnn'nTGC Cuts at: 0 404 1485 1551 1794 Size: 404 1081 66 243 Fragments arranged by size: 1081 404 243 66 BstXI CCAn_nnnn'nTGG Cuts at: 0 108 835 1794 Size: 108 727 959 Fragments arranged by size: 959 727 108 BstYI r'GATC_y Cuts at: 0 1268 1769 1794 Size: 1268 501 25 Fragments arranged by size: 1268 501 25 BtgI C'CryG_G Cuts at: 0 101 1428 1561 1794 Size: 101 1327 133 233 Fragments arranged by size: 1327 233 133 101 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1575 1794 Size: 1575 219 BtsI GCAGTG_nn' Cuts at: 0 140 1133 1426 1794 Size: 140 993 293 368 Fragments arranged by size: 993 368 293 140 EaeI y'GGCC_r Cuts at: 0 110 474 1794 Size: 110 364 1320 Fragments arranged by size: 1320 364 110 EarI CTCTTCn'nnn_ Cuts at: 0 1342 1794 Size: 1342 452 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1276 1336 1794 Size: 1276 60 458 Fragments arranged by size: 1276 458 60 EcoICRI GAG'CTC Cuts at: 0 1335 1794 Size: 1335 459 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 141 360 542 558 640 1045 1261 1336 Size: 141 219 182 16 82 405 216 75 Cuts at: 1336 1351 1624 1753 1794 Size: 15 273 129 41 Fragments arranged by size: 405 273 219 216 182 141 129 82 75 41 16 15 EcoNI CCTnn'n_nnAGG Cuts at: 0 1467 1794 Size: 1467 327 EcoO109I rG'GnC_Cy Cuts at: 0 46 366 545 773 774 1126 1305 1472 Size: 46 320 179 228 1 352 179 167 Cuts at: 1472 1704 1794 Size: 232 90 Fragments arranged by size: 352 320 232 228 179 179 167 90 46 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 885 1404 1794 Size: 885 519 390 Fragments arranged by size: 885 519 390 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 355 387 1213 1245 1794 Size: 355 32 826 32 549 Fragments arranged by size: 826 549 355 32 32 MscI TGG'CCA Cuts at: 0 112 1794 Size: 112 1682 MslI CAynn'nnrTG Cuts at: 0 166 836 1794 Size: 166 670 958 Fragments arranged by size: 958 670 166 MspA1I CmG'CkG Cuts at: 0 133 210 528 651 797 953 1563 1598 Size: 133 77 318 123 146 156 610 35 Cuts at: 1598 1621 1649 1794 Size: 23 28 145 Fragments arranged by size: 610 318 156 146 145 133 123 77 35 28 23 NaeI GCC'GGC Cuts at: 0 1042 1794 Size: 1042 752 NcoI C'CATG_G Cuts at: 0 101 1794 Size: 101 1693 NgoMIV G'CCGG_C Cuts at: 0 1040 1794 Size: 1040 754 NspI r_CATG'y Cuts at: 0 851 1794 Size: 851 943 PasI CC'CwG_GG Cuts at: 0 458 1701 1794 Size: 458 1243 93 Fragments arranged by size: 1243 458 93 PciI A'CATG_T Cuts at: 0 847 1794 Size: 847 947 PflMI CCAn_nnn'nTGG Cuts at: 0 651 1167 1794 Size: 651 516 627 Fragments arranged by size: 651 627 516 PfoI T'CCnGG_A Cuts at: 0 42 1160 1328 1794 Size: 42 1118 168 466 Fragments arranged by size: 1118 466 168 42 PmlI CAC'GTG Cuts at: 0 1614 1794 Size: 1614 180 PpuMI rG'GwC_Cy Cuts at: 0 46 1305 1472 1794 Size: 46 1259 167 322 Fragments arranged by size: 1259 322 167 46 PspOMI G'GGCC_C Cuts at: 0 544 773 1126 1794 Size: 544 229 353 668 Fragments arranged by size: 668 544 353 229 PstI C_TGCA'G Cuts at: 0 1421 1556 1583 1712 1794 Size: 1421 135 27 129 82 Fragments arranged by size: 1421 135 129 82 27 PvuII CAG'CTG Cuts at: 0 210 528 651 1598 1621 1649 1794 Size: 210 318 123 947 23 28 145 Fragments arranged by size: 947 318 210 145 123 28 23 SacI G_AGCT'C Cuts at: 0 1337 1794 Size: 1337 457 SacII CC_GC'GG Cuts at: 0 1564 1794 Size: 1564 230 SanDI GG'GwC_CC Cuts at: 0 46 1794 Size: 46 1748 SapI GCTCTTCn'nnn_ Cuts at: 0 1342 1794 Size: 1342 452 SfcI C'TryA_G Cuts at: 0 1417 1552 1579 1708 1794 Size: 1417 135 27 129 86 Fragments arranged by size: 1417 135 129 86 27 SmlI C'TyrA_G Cuts at: 0 718 1467 1726 1794 Size: 718 749 259 68 Fragments arranged by size: 749 718 259 68 StuI AGG'CCT Cuts at: 0 506 1400 1794 Size: 506 894 394 Fragments arranged by size: 894 506 394 StyI C'CwwG_G Cuts at: 0 101 758 1794 Size: 101 657 1036 Fragments arranged by size: 1036 657 101 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 658 717 1714 1770 1794 Size: 658 59 997 56 24 Fragments arranged by size: 997 658 59 56 24 TatI w'GTAC_w Cuts at: 0 899 1206 1794 Size: 899 307 588 Fragments arranged by size: 899 588 307 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 19 105 600 648 1794 Size: 19 86 495 48 1146 Fragments arranged by size: 1146 495 86 48 19 XmnI GAAnn'nnTTC Cuts at: 0 625 1794 Size: 625 1169 Enzymes that do cut: AcuI AflIII AlwNI ApaI ApaLI AvaI BaeI BanI BanII BbvCI BcgI BciVI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BstAPI BstXI BstYI BtgI BtgZI BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MscI MslI MspA1I NaeI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SfcI SmlI StuI StyI TaqII TatI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI AloI ApoI AscI AseI AsiSI AvrII BamHI BbeI BbsI BclI BfrBI BglI BmtI BplI BsaI BsaBI BsaHI BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EcoRI EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI ZraI