Restriction map for
pFN21AB6928
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# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BlpI BmyI
BpmI Bpu1102I BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmBI BsmI BsoBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BsrI BssHII
BssSI BstAPI BstBI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRV EcoT22I
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 213 GTATAC GT^[AC][GT]AC
AccII 145 CGCG CG^CG
AcsI 199 GAATTC [GA]^AATT[CT]
AfaI 388 GTAC GT^AC
ApoI 199 GAATTC [GA]^AATT[CT]
AvaII 29 GGTCC G^G[AT]CC
BbsI 283 GAAGAC GAAGAC(2/6)
BfaI 463 CTAG C^TAG
BglI 406 GCCCCGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 518 AGATCT A^GATCT
BpuAI 283 GAAGAC GAAGAC(2/6)
BsaAI 389 TACGTG [CT]AC^GT[GA]
BsaI 131 GAGACC GGTCTC(1/5)
Bst1107I 213 GTATAC GTA^TAC
BstEII 120 GGTGACC G^GT[ACGT]ACC
BstPI 120 GGTGACC G^GT[ACGT]ACC
BstUI 145 CGCG CG^CG
BstYI 518 AGATCT [GA]^GATC[CT]
BstZ17I 213 GTATAC GTA^TAC
Cac8I 294 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 146 GCGC GCG^C
DraII 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 142 GACCGCGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 222 TGGCCG [CT]^GGCC[GA]
EarI 565 GAAGAG CTCTTC(1/4)
EcoO109I 28 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 120 GGTGACC G^GT[ACGT]ACC
EcoRI 199 GAATTC G^AATTC
FauI 101 GCGGG CCCGC(4/6)
HhaI 146 GCGC GCG^C
HinP1I 146 GCGC G^CGC
HphI 120 GGTGA GGTGA(8/7)
Ksp632I 565 GAAGAG CTCTTC(1/4)
MaeI 463 CTAG C^TAG
MaeII 390 ACGT A^CGT
MflI 518 AGATCT [GA]^GATC[CT]
MspA1I 252 CAGCTG C[AC]G^C[GT]G
MvnI 145 CGCG CG^CG
MwoI 406 GCCCCGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 69 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 568 GAGTC GAGTC(4/5)
PpuMI 28 GGGTCCC [GA]G^G[AT]CC[CT]
PstI 550 CTGCAG CTGCA^G
PvuII 252 CAGCTG CAG^CTG
RsaI 388 GTAC GT^AC
SfaNI 113 GATGC GCATC(5/9)
SmlI 640 CTCAAG C^T[CT][GA]AG
TspRI 601 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 69 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 462 TCTAGA T^CTAGA
XhoII 518 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 144 CCGC CCGC(-3/-1)
101 GCGG
AlwI 350 GATCC GGATC(4/5)
13 GGATC
BseRI 180 CTCCTC GAGGAG(10/8)
442 GAGGAG
BsmAI 131 GAGAC GTCTC(1/5)
570 GTCTC
BspMI 56 ACCTGC ACCTGC(4/8)
593 ACCTGC
Cfr13I 29 GGTCC G^G[ACGT]CC
78 GGCCC
DdeI 183 CTCAG C^T[ACGT]AG
325 CTGAG
DsaI 19 CCATGG C^C[GA][CT]GG
219 CCATGG
MaeIII 121 GTGAC ^GT[ACGT]AC
379 GTCAC
NcoI 19 CCATGG C^CATGG
219 CCATGG
Sau96I 29 GGTCC G^G[ACGT]CC
78 GGCCC
SfcI 431 CTGTAG C^T[GA][CT]AG
550 CTGCAG
Tsp45I 121 GTGAC ^GT[GC]AC
379 GTCAC
Tsp509I 200 AATT ^AATT
634 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI BbvI BcnI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MnlI MspI MvaI
NciI NdeII NlaIII NlaIV Sau3AI
ScrFI StyI TaqI TfiI TseI
TthHB8I