(Linear) (Six-base) MAPSORT of: KIBB6928.seq  Check: 5047  from: 1  to: 681



KIBB6928.orf

 With 182 enzymes: SgfI * 

                        April 6, 2009 09:17  ..

AccI GT'mk_AC

Cuts at:      0     214     681 
   Size:        214     467
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     681 
   Size:        670      11
 
ApoI r'AATT_y

Cuts at:      0     199     681 
   Size:        199     482
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     290     681 
   Size:        290     391
 
BglI GCCn_nnn'nGGC

Cuts at:      0     412     681 
   Size:        412     269
 
BglII A'GATC_T

Cuts at:      0     518     681 
   Size:        518     163
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     625     681 
   Size:        625      56
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     125     681 
   Size:        125     556
 
BsaAI yAC'GTr

Cuts at:      0     391     681 
   Size:        391     290
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     171     457     681 
   Size:        171     286     224

  Fragments arranged by size: 

                 286     224     171
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      65     602     681 
   Size:         65     537      79

  Fragments arranged by size: 

                 537      79      65
 
BstEII G'GTnAC_C

Cuts at:      0     120     681 
   Size:        120     561
 
BstYI r'GATC_y

Cuts at:      0     518     681 
   Size:        518     163
 
BstZ17I GTA'TAC

Cuts at:      0     215     681 
   Size:        215     466
 
BtgI C'CryG_G

Cuts at:      0      19     219     681 
   Size:         19     200     462

  Fragments arranged by size: 

                 462     200      19
 
BtsI GCAGTG_nn'

Cuts at:      0     600     681 
   Size:        600      81
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     148     681 
   Size:        148     533
 
EaeI y'GGCC_r

Cuts at:      0     222     681 
   Size:        222     459
 
EarI CTCTTCn'nnn_

Cuts at:      0     560     681 
   Size:        560     121
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     670     681 
   Size:        670      11
 
EcoO109I rG'GnC_Cy

Cuts at:      0      29     681 
   Size:         29     652
 
EcoRI G'AATT_C

Cuts at:      0     199     681 
   Size:        199     482
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     553     585     681 
   Size:        553      32      96

  Fragments arranged by size: 

                 553      96      32
 
MspA1I CmG'CkG

Cuts at:      0     254     681 
   Size:        254     427
 
NcoI C'CATG_G

Cuts at:      0      19     219     681 
   Size:         19     200     462

  Fragments arranged by size: 

                 462     200      19
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      75     681 
   Size:         75     606
 
PfoI T'CCnGG_A

Cuts at:      0     352     681 
   Size:        352     329
 
PpuMI rG'GwC_Cy

Cuts at:      0      29     681 
   Size:         29     652
 
PsiI TTA'TAA

Cuts at:      0     590     681 
   Size:        590      91
 
PstI C_TGCA'G

Cuts at:      0     554     681 
   Size:        554     127
 
PvuII CAG'CTG

Cuts at:      0     254     681 
   Size:        254     427
 
SanDI GG'GwC_CC

Cuts at:      0      29     681 
   Size:         29     652
 
SfcI C'TryA_G

Cuts at:      0     431     550     681 
   Size:        431     119     131

  Fragments arranged by size: 

                 431     131     119
 
SmlI C'TyrA_G

Cuts at:      0     640     681 
   Size:        640      41
 
StyI C'CwwG_G

Cuts at:      0      19     219     239     681 
   Size:         19     200      20     442

  Fragments arranged by size: 

                 442     200      20      19
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     250     282     681 
   Size:        250      32     399

  Fragments arranged by size: 

                 399     250      32
 
XbaI T'CTAG_A

Cuts at:      0     462     681 
   Size:        462     219
 

 Enzymes that do cut:

     AccI     AcuI     ApoI     BbsI     BglI    BglII    BpuEI     BsaI
    BsaAI    BseRI    BspMI   BstEII    BstYI  BstZ17I     BtgI     BtsI
     DrdI     EaeI     EarI  Eco57MI EcoO109I    EcoRI    Hin4I   MspA1I
     NcoI    PflMI     PfoI    PpuMI     PsiI     PstI    PvuII    SanDI
     SfcI     SmlI     StyI     TstI     XbaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI    BstXI   Bsu36I    BtgZI     ClaI
    CspCI     DraI   DraIII     EagI     EciI  EcoICRI    EcoNI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PshAI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI  Tth111I     XcmI     XhoI     XmaI     XmnI     ZraI